Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,685,527 A→G 100% G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5270AG100.0% 258.1 / NA 69G354G (GGT→GGCfumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/29);  total (40/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAC  >  NC_000913/1685381‑1685669
                                                                                                                                                  |                                                                                                                                              
ttGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGcc                                                                                                                                              >  1:24579/1‑149 (MQ=255)
    gTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGaa                                                                                                                                          >  1:487581/1‑149 (MQ=255)
       attgattgATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgcg                                                                                                                                       >  4:625151/1‑149 (MQ=255)
       attgattgATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgcg                                                                                                                                       >  1:354079/1‑149 (MQ=255)
       attgattgATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATCGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgcg                                                                                                                                       >  4:21648/1‑149 (MQ=255)
         tgattgatTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcc                                                                                                                                     <  2:254118/149‑1 (MQ=255)
           attgattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccc                                                                                                                                   >  2:377375/1‑149 (MQ=255)
               attCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCAt                                                                                                                               >  4:244939/1‑149 (MQ=255)
               attCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCAt                                                                                                                               >  1:159931/1‑149 (MQ=255)
                ttCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATg                                                                                                                              >  2:5231/1‑149 (MQ=255)
                        cGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGc                                                                                                                      <  2:487581/149‑1 (MQ=255)
                               gTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcg                                                                                                               >  4:291086/1‑149 (MQ=255)
                                ttCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcgt                                                                                                              >  4:28461/1‑149 (MQ=255)
                                 tCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGtt                                                                                                             >  1:620678/1‑149 (MQ=255)
                                  cAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTc                                                                                                            <  4:110858/149‑1 (MQ=255)
                                     tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTcccc                                                                                                         >  2:438008/1‑149 (MQ=255)
                                      aCCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCa                                                                                                        >  3:11734/1‑149 (MQ=255)
                                       cccACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                       >  1:636082/1‑149 (MQ=255)
                                        ccACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATc                                                                                                                        <  2:577099/131‑1 (MQ=255)
                                        ccACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATc                                                                                                                        >  1:577099/1‑131 (MQ=255)
                                        ccACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATc                                                                                                      >  1:677402/1‑149 (MQ=255)
                                         cACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCAt                                                                                                                               >  4:559656/1‑123 (MQ=255)
                                         cACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCAt                                                                                                                               <  3:559656/123‑1 (MQ=255)
                                                 aGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                             >  3:486635/1‑149 (MQ=255)
                                                 aGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                             >  2:509450/1‑149 (MQ=255)
                                                     tttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCaga                                                                                         <  2:159931/149‑1 (MQ=255)
                                                     tttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCaga                                                                                         >  3:289625/1‑149 (MQ=255)
                                                        gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                      <  1:377797/149‑1 (MQ=255)
                                                         ttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCAt                                                                                     >  1:660014/1‑149 (MQ=255)
                                                         ttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCAt                                                                                     >  2:628301/1‑149 (MQ=255)
                                                             aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGtt                                                                                 <  3:581035/149‑1 (MQ=255)
                                                                tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATCGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAt                                                                              <  3:21648/149‑1 (MQ=255)
                                                                 ttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATg                                                                             <  1:5231/149‑1 (MQ=255)
                                                                          aTCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACt                                                                    >  3:254011/1‑149 (MQ=255)
                                                                          aTCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACt                                                                    >  2:550753/1‑149 (MQ=255)
                                                                               ccAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGtt                                                               <  3:291086/149‑1 (MQ=255)
                                                                                cAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTg                                                              <  2:677402/149‑1 (MQ=255)
                                                                                 aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgccgccatc                                                                                                          >  1:38251/1‑99 (MQ=255)
                                                                                 aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                                                             <  1:550753/149‑1 (MQ=255)
                                                                                  gCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                            <  2:24579/149‑1 (MQ=255)
                                                                                  gCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                            >  3:594884/1‑149 (MQ=255)
                                                                                  gCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                            >  2:83403/1‑149 (MQ=255)
                                                                                    aaGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                                                             >  4:342557/1‑146 (MQ=255)
                                                                                    aaGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                                                             <  3:342557/146‑1 (MQ=255)
                                                                                    aaGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGtt                                                          <  1:514798/149‑1 (MQ=255)
                                                                                    aaGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGtt                                                          >  4:132479/1‑149 (MQ=255)
                                                                                       cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCAc                                                       <  1:438008/149‑1 (MQ=255)
                                                                                          aCCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACttt                                                    >  3:680390/1‑149 (MQ=255)
                                                                                            cGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTcc                                                  >  2:504640/1‑149 (MQ=255)
                                                                                             gATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATc                                                                                                                        >  1:312109/1‑78 (MQ=255)
                                                                                             gATTGCAGGAAATTGTGGATCACCAGTGGACGGAAGACGTTCAGTGCAAAGTTGCCGGAAGCGCCCCCCATGTTGCTc                                                                                                                        <  2:312109/78‑1 (MQ=255)
                                                                                                     gAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTg                                                              >  3:238462/1‑128 (MQ=255)
                                                                                                     gAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTg                                                              <  4:238462/128‑1 (MQ=255)
                                                                                                     gAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGa                                         >  1:488673/1‑149 (MQ=255)
                                                                                                     gAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGa                                         >  3:616707/1‑149 (MQ=255)
                                                                                                         ttGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGa                                     <  1:509450/149‑1 (MQ=255)
                                                                                                                  aCCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCgg                            >  1:296333/1‑149 (MQ=255)
                                                                                                                      ttGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCa                        <  1:628301/149‑1 (MQ=255)
                                                                                                                        ggACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCAtt                      <  2:488673/149‑1 (MQ=255)
                                                                                                                             gAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCg                 <  3:244939/149‑1 (MQ=255)
                                                                                                                              aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCgg                <  1:377375/149‑1 (MQ=255)
                                                                                                                              aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCgg                <  4:289625/149‑1 (MQ=255)
                                                                                                                                    ttcagttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAg                                    >  4:592713/1‑123 (MQ=255)
                                                                                                                                    ttcagttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAg                                    <  3:592713/123‑1 (MQ=255)
                                                                                                                                     tcagttcaAAGTTGCCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTgaga        <  4:18178/149‑1 (MQ=255)
                                                                                                                                         ttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTga          >  2:202805/1‑144 (MQ=255)
                                                                                                                                         ttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTAGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTga          <  1:202805/144‑1 (MQ=255)
                                                                                                                                           caAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCa   >  1:628643/1‑149 (MQ=255)
                                                                                                                                            aaaGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAc  <  2:296333/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
TTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAC  >  NC_000913/1685381‑1685669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: