Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,747,029 G→C 14.8% intergenic (+24/‑102) ydhR → / → ydhS putative monooxygenase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,747,0290GC14.8% 160.8 / 20.6 54intergenic (+24/‑102)ydhR/ydhSputative monooxygenase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (25/21);  new base C (4/4);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

GCGCCCTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAT  >  NC_000913/1746881‑1747171
                                                                                                                                                    |                                                                                                                                              
gcgcCCTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAg                                                                                                                                                <  1:678599/149‑1 (MQ=255)
    ccTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCAGTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGcc                                                                                                                                            >  4:475380/1‑149 (MQ=255)
         ccTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCtttt                                                                                                                                       <  3:517694/149‑1 (MQ=255)
            aTCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCAt                                                                                                                                    <  1:603709/149‑1 (MQ=255)
            aTCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttga                                                                                                                                       <  1:256415/146‑3 (MQ=255)
            aTCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttga                                                                                                                                       >  2:256415/1‑144 (MQ=255)
             tCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATc                                                                                                                                   <  3:211258/149‑1 (MQ=255)
                 gAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTg                                                                                                                               >  2:286671/1‑149 (MQ=255)
                    aaaCATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCt                                                                                                                            <  1:286671/149‑1 (MQ=255)
                    aaaCATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                        <  3:511854/137‑2 (MQ=255)
                    aaaCATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                        >  4:511854/1‑136 (MQ=255)
                        aTACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGaaa                                                                                                                        <  3:475380/149‑1 (MQ=255)
                                        aaaTCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTaaa                                                                                                        >  4:22764/1‑149 (MQ=255)
                                         aaTCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTaaaa                                                                                                       <  3:686928/149‑1 (MQ=255)
                                             tcGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAAtct                                                                                                   <  2:390232/149‑1 (MQ=255)
                                                      aGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATcc                                                                                                                                  >  1:40530/1‑109 (MQ=255)
                                                      aGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATcc                                                                                                                                  <  2:40530/109‑1 (MQ=255)
                                                        gAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACtt                                                                                        >  3:86819/1‑149 (MQ=255)
                                                        gAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                        >  4:303758/1‑100 (MQ=255)
                                                        gAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                        >  4:531836/1‑100 (MQ=255)
                                                        gAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                        <  3:531836/101‑2 (MQ=255)
                                                        gAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                        <  3:303758/101‑2 (MQ=255)
                                                                 gCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATaa                                                                               >  1:640945/1‑149 (MQ=255)
                                                                 gCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATaa                                                                               <  3:22764/149‑1 (MQ=255)
                                                                 gCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATaa                                                                               >  3:223919/1‑149 (MQ=255)
                                                                   cAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAaca                                                                             >  1:275757/1‑149 (MQ=255)
                                                                              aTGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGaa                                                                  >  4:275750/1‑149 (MQ=255)
                                                                              aTGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGaa                                                                  <  4:86819/149‑1 (MQ=255)
                                                                               tGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGaaa                                                                 >  4:107623/1‑149 (MQ=255)
                                                                                   aaTGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTtc                                                                                                                    <  2:176020/94‑1 (MQ=255)
                                                                                   aaTGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTtc                                                                                                                    >  1:176020/1‑94 (MQ=255)
                                                                                             ttAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgt                                                   >  2:610295/1‑149 (MQ=255)
                                                                                             ttAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgt                                                   >  2:588164/1‑149 (MQ=255)
                                                                                               aGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaa                                                 <  2:275757/149‑1 (MQ=255)
                                                                                                 tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAAtt                                                                      >  1:186342/1‑126 (MQ=255)
                                                                                                 tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAAtt                                                                      <  2:186342/126‑1 (MQ=255)
                                                                                                 tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaagt                                               >  1:254966/1‑149 (MQ=255)
                                                                                                 tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaagt                                               >  1:647907/1‑149 (MQ=255)
                                                                                                   aaaTCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaagtaa                                             >  2:674352/1‑149 (MQ=255)
                                                                                                     atcaatcaGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaa                                                 <  3:269387/143‑1 (MQ=255)
                                                                                                     atcaatcaGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaa                                                 >  4:269387/1‑143 (MQ=255)
                                                                                                      tcaatcaGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAATACTAAATTCGAAAGAATGCGTACAGGTAAGTAACaa                                          >  4:467833/1‑149 (MQ=255)
                                                                                                      tcaatcaGGCAAAACTCGCATGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCTCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACaa                                          <  2:496700/149‑1 (MQ=255)
                                                                                                          tcaGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgt                                                   >  2:673698/1‑136 (MQ=255)
                                                                                                          tcaGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgt                                                   <  1:673698/136‑1 (MQ=255)
                                                                                                                 aaaCTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAGAAtt                               <  1:588164/149‑1 (MQ=255)
                                                                                                                 aaaCTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAAtt                               <  1:610295/149‑1 (MQ=255)
                                                                                                                  gtttCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTg                              >  2:457719/4‑149 (MQ=255)
                                                                                                                     tCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTa                           >  1:576897/1‑149 (MQ=255)
                                                                                                                             cAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGt                   <  4:223919/149‑1 (MQ=255)
                                                                                                                              aGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTg                  >  2:87676/1‑149 (MQ=255)
                                                                                                                                  tttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCgg              <  1:457719/149‑1 (MQ=255)
                                                                                                                                         aaGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACg       >  3:253567/1‑149 (MQ=255)
                                                                                                                                          aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg      >  3:563901/1‑149 (MQ=255)
                                                                                                                                              cGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAt  <  3:107623/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GCGCCCTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAT  >  NC_000913/1746881‑1747171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑