Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,764,694 G→C 11.4% intergenic (‑308/+19) sufA ← / ← rydB Fe‑S cluster assembly protein/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,764,6940GC11.4% 136.8 / 10.2 44intergenic (‑308/+19)sufA/rydBFe‑S cluster assembly protein/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base G (25/14);  new base C (3/2);  total (28/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

ATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAA  >  NC_000913/1764554‑1764835
                                                                                                                                             |                                                                                                                                             
aTCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGc                                                                                                                                       >  3:261469/1‑149 (MQ=255)
    cATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTaa                                                                                                                                   <  2:390746/149‑1 (MQ=255)
    cATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCG‑CACAGACTTaa                                                                                                                                   <  1:173422/149‑1 (MQ=255)
        cttAGATAATAACCATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGt                                                                                                                               >  1:689588/2‑149 (MQ=255)
             aTAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGt                                                                                                                               >  4:521317/1‑144 (MQ=255)
             aTAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGGTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGt                                                                                                                               <  3:521317/144‑1 (MQ=255)
              tAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTAc                                                                                                                         <  4:261469/149‑1 (MQ=255)
                    ttattaTCTAAGGAGCATTAAGGCTGTTATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAGATAAGATAACCCTTAAGTCTGTG‑CGACACAGGCTTaa                                                                                                                                   <  1:23571/133‑1 (MQ=255)
                    ttattaTCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTaa                                                                                                                                   >  2:23571/1‑133 (MQ=255)
                          tCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGgcgc                                                                                                             >  4:682990/1‑149 (MQ=255)
                          tCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGgcgc                                                                                                             >  4:514799/1‑149 (MQ=255)
                          tCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTG‑TCGCACAGACTTaa                                                                                                                                   >  3:461700/1‑127 (MQ=39)
                          tCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTG‑TCGCACAGACTTaa                                                                                                                                   <  4:461700/127‑1 (MQ=39)
                               ggAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAtc                                                                                                        >  3:225025/1‑149 (MQ=255)
                                    aTTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTATGGGTTTCTACCCCATCCGGCGCTTATCTCCgg                                                                                                   >  4:564692/1‑149 (MQ=255)
                                     ttAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGc                                                                                                  >  4:603593/1‑149 (MQ=255)
                                                   aaaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCtt                                                                                    >  4:205931/1‑149 (MQ=255)
                                                               ttGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAgg                                                                        >  3:185785/1‑149 (MQ=255)
                                                                     tGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGGCTTTG‑CGACACAGGCTTAAGGGTTTCTACCCCCACCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGAt                                                                  <  3:682990/149‑1 (MQ=255)
                                                                       aaaaTGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATaa                                                                <  2:689588/149‑1 (MQ=255)
                                                                              cTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAACAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGGTCTTGAAGGGGCGATAAGaataat                                                         <  3:651224/149‑1 (MQ=255)
                                                                               tATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtc                                                        <  3:414966/149‑1 (MQ=255)
                                                                                 tttAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtctc                                                      <  3:514799/149‑1 (MQ=255)
                                                                                      gagaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATaaa                                                 >  3:154278/1‑149 (MQ=255)
                                                                                              tAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAccc                                         >  1:306549/1‑149 (MQ=255)
                                                                                                aaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgc                                       >  1:287381/1‑149 (MQ=255)
                                                                                                 aaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgcc                                      >  4:149974/1‑149 (MQ=255)
                                                                                                        cAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTT‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTaa                               <  3:564692/149‑1 (MQ=255)
                                                                                                             ttCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACaa                          >  3:610047/1‑149 (MQ=255)
                                                                                                             ttCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACaa                          >  2:367534/1‑149 (MQ=255)
                                                                                                                aGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACt                       >  3:172841/1‑149 (MQ=255)
                                                                                                                 gAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTg                      >  2:25766/1‑149 (MQ=255)
                                                                                                                 gAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTCTAACACAAACTg                      >  3:427084/1‑149 (MQ=255)
                                                                                                                  aaaTAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGc                     <  4:154278/149‑1 (MQ=255)
                                                                                                                        gAAAACCCTTAAGTCTGTG‑TGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAg               >  2:446908/1‑149 (MQ=255)
                                                                                                                        gAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGGTTAg               >  1:669261/1‑149 (MQ=255)
                                                                                                                        gAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAg               >  2:27676/1‑149 (MQ=255)
                                                                                                                           aaCCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtat            >  4:12117/1‑149 (MQ=255)
                                                                                                                              ccTTAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtattat         >  1:358279/1‑149 (MQ=255)
                                                                                                                               cTTAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTa                >  3:305076/1‑141 (MQ=255)
                                                                                                                               cTTAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTa                <  4:305076/141‑1 (MQ=255)
                                                                                                                                ttAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTAttt       >  3:101795/1‑149 (MQ=255)
                                                                                                                                  aaGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTAttttt     <  4:610047/149‑1 (MQ=255)
                                                                                                                                     tCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGGTTAGTATTATTTTTGaa  <  2:669261/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                             
ATCTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAA  >  NC_000913/1764554‑1764835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑