Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,981,814 T→A 5.6% T192S (ACG→TCG)  otsB ← trehalose‑6‑phosphate phosphatase, biosynthetic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,981,8140TA 5.6% 248.1 / 5.0 72T192S (ACG→TCG) otsBtrehalose‑6‑phosphate phosphatase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base T (27/41);  new base A (2/2);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

CCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAAT  >  NC_000913/1981671‑1981962
                                                                                                                                               |                                                                                                                                                    
ccAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGcc                                                                                                                                                 <  4:632765/149‑1 (MQ=255)
    cTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGAt                                                                                                                                             >  3:377740/1‑149 (MQ=255)
    cTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGAt                                                                                                                                             >  3:428383/1‑149 (MQ=255)
     tCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATa                                                                                                                                            >  1:58851/1‑149 (MQ=255)
     tCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATa                                                                                                                                            >  4:36955/1‑149 (MQ=255)
     tCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgtat                                                                                                                                            <  1:138053/149‑4 (MQ=255)
        aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg                                                                                                                                         <  2:232895/149‑1 (MQ=255)
        aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg                                                                                                                                         <  1:269995/149‑1 (MQ=255)
        aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg                                                                                                                                         <  3:460837/149‑1 (MQ=255)
        aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg                                                                                                                                         >  3:517002/1‑149 (MQ=255)
          aCATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAggg                                                                                                                                       >  2:301211/1‑149 (MQ=255)
          aCATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAggg                                                                                                                                       >  3:584490/1‑149 (MQ=255)
            aTCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAg                                                                                                                                     >  4:546142/1‑149 (MQ=255)
            aTCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAg                                                                                                                                     <  3:300053/149‑1 (MQ=255)
                  cacaCCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCt                                                                                                                               <  1:273885/149‑1 (MQ=255)
                  cacaCCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCt                                                                                                                               <  4:428383/149‑1 (MQ=255)
                   acacCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTg                                                                                                                              >  1:508773/1‑149 (MQ=255)
                      cccGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCAt                                                                                                                           <  3:523975/149‑1 (MQ=255)
                              tCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTg                                                                                                                   <  3:620598/149‑1 (MQ=255)
                              tCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTg                                                                                                                   >  2:613146/1‑149 (MQ=255)
                                 ccATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgta                                                                                                                                             <  4:172374/120‑3 (MQ=255)
                                 ccATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgta                                                                                                                                             >  3:172374/1‑118 (MQ=255)
                                        gacTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTc                                                                                                         >  2:306222/3‑149 (MQ=255)
                                            gAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCt                                                                                                     <  1:436993/149‑1 (MQ=255)
                                               ttGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa                                                                                                  <  1:214100/149‑1 (MQ=255)
                                               ttGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa                                                                                                  <  2:414733/149‑1 (MQ=255)
                                                tGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTAc                                                                                                 <  1:367350/149‑1 (MQ=255)
                                                tGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTAc                                                                                                 <  3:180060/149‑1 (MQ=255)
                                                 gCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACt                                                                                                <  3:97687/149‑1 (MQ=255)
                                                  cACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTg                                                                                               <  4:547092/149‑1 (MQ=255)
                                                    ccTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGa                                                                                                                                      <  4:457757/108‑1 (MQ=255)
                                                    ccTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGa                                                                                                                                      >  3:457757/1‑108 (MQ=255)
                                                    ccTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGt                                                                                             >  1:659544/1‑149 (MQ=255)
                                                     cTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTa                                                                                            <  1:306222/149‑1 (MQ=255)
                                                      tgtgCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTAc                                                                                           <  4:517002/149‑1 (MQ=255)
                                                         gCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATaaa                                                                                                                                          >  3:516724/1‑99 (MQ=255)
                                                         gCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATaaa                                                                                                                                          <  4:516724/99‑1 (MQ=255)
                                                           cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGa                                                                                                                                      <  2:516290/101‑1 (MQ=255)
                                                           cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGa                                                                                                                                      >  1:516290/1‑101 (MQ=255)
                                                           cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCtctc                                                                                      >  4:249476/1‑149 (MQ=255)
                                                           cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCtctc                                                                                      <  3:249476/149‑1 (MQ=255)
                                                              tttttACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGt                                                                                   <  2:189995/149‑1 (MQ=255)
                                                                  tACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAAtt                                                                                                              <  1:98895/118‑1 (MQ=255)
                                                                  tACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAAtt                                                                                                              >  2:98895/1‑118 (MQ=255)
                                                                   aCTGACATTCCGCACCGTCGGTTAACGACTGCGTAGCCAGATTCATCGGTTAAATCATAGCCCAGAAATACGGGCGTTCTCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCCGTACTTGCACCTCTCGGtttctt                                                                              <  4:197571/149‑4 (MQ=255)
                                                                    cTGACATTCCGCCCAGGCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtc                                                                             >  3:503164/1‑149 (MQ=255)
                                                                     tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGttt                                                                                 <  1:460554/144‑1 (MQ=255)
                                                                     tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGttt                                                                                 >  2:460554/1‑144 (MQ=255)
                                                                       aCATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCg                                                                          >  2:342595/1‑149 (MQ=255)
                                                                       aCATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCg                                                                          <  1:301211/149‑1 (MQ=255)
                                                                        cATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGa                                                                         <  2:58851/149‑1 (MQ=255)
                                                                         aTTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGac                                                                        <  2:626020/149‑1 (MQ=255)
                                                                         aTTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGac                                                                        >  3:92907/1‑149 (MQ=255)
                                                                          ttCCGCCCAGTCGGTTAACGACTGCGATGCCAGATTCATCGCTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGaca                                                                       >  1:489372/1‑149 (MQ=255)
                                                                          ttCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGa                                                                         <  4:579344/147‑1 (MQ=255)
                                                                          ttCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGa                                                                         >  3:579344/1‑147 (MQ=255)
                                                                           tCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGacaa                                                                      <  3:36955/149‑1 (MQ=255)
                                                                               cccAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAacaca                                                                  <  4:584490/149‑1 (MQ=255)
                                                                                     cGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTcc                                                            <  4:40998/149‑1 (MQ=255)
                                                                                      ggTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTccc                                                           >  3:103195/1‑149 (MQ=255)
                                                                                       gTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCct                                                          >  2:139295/1‑149 (MQ=255)
                                                                                                         aGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTtgtg                                        <  4:457883/149‑1 (MQ=255)
                                                                                                                tCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGAt                                 <  4:377740/149‑1 (MQ=255)
                                                                                                                  ggTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCt                               <  1:613146/149‑1 (MQ=255)
                                                                                                                    ttAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGa                             <  4:92907/149‑1 (MQ=255)
                                                                                                                    ttAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGa                             >  1:674573/1‑149 (MQ=255)
                                                                                                                     tAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAg                            <  2:611882/149‑1 (MQ=255)
                                                                                                                        atcatcGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGttt                                                                                 >  2:34883/1‑93 (MQ=255)
                                                                                                                        atcatcGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGttt                                                                                 <  1:34883/93‑1 (MQ=255)
                                                                                                                         tcatcGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAAt                        <  2:659544/149‑1 (MQ=255)
                                                                                                                                   ctttatcGGGCGAACGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCt              >  2:126604/7‑149 (MQ=255)
                                                                                                                                         cGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTc        <  4:503164/149‑1 (MQ=255)
                                                                                                                                               ttCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAAt  <  4:161065/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAAT  >  NC_000913/1981671‑1981962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑