Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,232,804 A→T 8.2% intergenic (+76/‑74) cdd → / → sanA cytidine/deoxycytidine deaminase/DUF218 superfamily vancomycin high temperature exclusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,232,8040AT 8.2% 243.6 / 11.2 73intergenic (+76/‑74)cdd/sanAcytidine/deoxycytidine deaminase/DUF218 superfamily vancomycin high temperature exclusion protein
Reads supporting (aligned to +/- strand):  ref base A (34/33);  new base T (3/3);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

GGATGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGAT  >  NC_000913/2232662‑2232949
                                                                                                                                              |                                                                                                                                                 
ggATGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGc                                                                                                                                             >  2:5920/1‑149 (MQ=255)
  aTGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACTGTTTGCCg                                                                                                                                           <  3:426997/149‑1 (MQ=255)
    gCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGtt                                                                                                                                         <  1:536399/149‑1 (MQ=255)
     ccACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGttt                                                                                                                                        <  4:628698/149‑1 (MQ=255)
     ccACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGttt                                                                                                                                        <  3:543593/149‑1 (MQ=255)
      cACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACTGTTTGCCgggaa                                                                                                                                       <  3:408592/149‑5 (MQ=255)
      cACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTc                                                                                                                                       <  4:167170/149‑1 (MQ=255)
       aCCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCt                                                                                                                                      >  1:257725/1‑149 (MQ=255)
        ccTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCtt                                                                                                                                     <  4:129047/149‑1 (MQ=255)
          tCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTgc                                                                                                                                   <  2:242341/149‑1 (MQ=255)
          tCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTgc                                                                                                                                   <  1:262122/149‑1 (MQ=255)
          tCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTgc                                                                                                                                   <  1:5920/149‑1 (MQ=255)
             gCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCa                                                                                                                                >  4:269812/1‑149 (MQ=255)
             gCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCa                                                                                                                                >  4:60509/1‑149 (MQ=255)
               aaCGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCaaa                                                                                                                              >  3:172539/1‑149 (MQ=255)
                  gCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAAcc                                                                                                                           >  4:318874/1‑149 (MQ=255)
                   cTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCa                                                                                                                          >  3:28161/1‑149 (MQ=255)
                    tGAAAGCTCTCGGCTGTCACAGTATCGACCGCGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTACGGGTTGCCAGTCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAg                                                                                                                         <  2:287250/149‑1 (MQ=255)
                    tGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAg                                                                                                                         >  1:94200/1‑149 (MQ=255)
                     gAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGc                                                                                                                        <  4:314286/149‑1 (MQ=255)
                        aGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCggg                                                                                                                     <  1:42263/149‑1 (MQ=255)
                        aGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCggg                                                                                                                     >  1:318523/1‑149 (MQ=255)
                             tcGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACa                                                                                                                                                   <  2:309612/114‑1 (MQ=255)
                             tcGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACa                                                                                                                                                   >  1:309612/1‑114 (MQ=255)
                               ggCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTa                                                                                                              <  3:617589/149‑1 (MQ=255)
                               ggCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTa                                                                                                              <  4:637690/149‑1 (MQ=255)
                                  tGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGcc                                                                                                           >  2:679973/1‑149 (MQ=255)
                                   gTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCt                                                                                                          <  3:236940/149‑1 (MQ=255)
                                    tCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACTGTTTGCCgg                                                                                                                                          <  4:178054/116‑2 (MQ=255)
                                    tCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACTGTTTGCCgg                                                                                                                                          >  3:178054/1‑115 (MQ=255)
                                      acaGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGAt                                                                                                       <  1:413681/149‑1 (MQ=255)
                                              gACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAAttt                                                                                               >  1:86097/1‑149 (MQ=255)
                                                ccGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTc                                                                                             <  3:318874/149‑1 (MQ=255)
                                                 cGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCaaaa                                                                                                                             >  1:594500/1‑116 (MQ=255)
                                                 cGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCaaaa                                                                                                                             <  2:594500/116‑1 (MQ=255)
                                                   aGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCtt                                                                                          <  2:541066/149‑1 (MQ=255)
                                                        ttCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCgg                                                                                                                      <  1:151597/116‑1 (MQ=255)
                                                        ttCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCgg                                                                                                                      >  2:151597/1‑116 (MQ=255)
                                                        ttCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATc                                                                                     >  3:21328/1‑149 (MQ=255)
                                                          ctcGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGa                                                                                   >  2:508280/1‑149 (MQ=255)
                                                          ctcGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGa                                                                                   >  3:646561/1‑149 (MQ=255)
                                                               ttAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGtctc                                                                              >  1:26629/1‑149 (MQ=255)
                                                                   gCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGc                                                                          >  2:690224/1‑149 (MQ=255)
                                                                   gCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGc                                                                          >  2:460172/1‑149 (MQ=255)
                                                                   gCCAGGTGCCGGATGCGGCGTGAACGCCTTGTGCGGCTTGCCAGCCCTCTCCTGGGGTCGAAATTCCCCGCAAACAGTTTGCCGATTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGc                                                                          >  2:566048/1‑149 (MQ=255)
                                                                     cTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCAt                                                                        <  3:125083/149‑1 (MQ=255)
                                                                           ccGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTaaa                                                                  <  4:172539/149‑1 (MQ=255)
                                                                                 gcggcgTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCgt                                                            <  2:318523/149‑1 (MQ=255)
                                                                                        gAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTc                                                     >  2:227746/1‑149 (MQ=255)
                                                                                         aaCGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCa                                                    >  2:642243/1‑149 (MQ=255)
                                                                                         aaCGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCa                                                    >  1:459860/1‑149 (MQ=255)
                                                                                           cGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGc                                                  <  4:46875/149‑1 (MQ=255)
                                                                                              cTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTg                                               >  1:568947/1‑149 (MQ=255)
                                                                                                  tCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAGAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCCGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAg                                           >  4:121160/1‑149 (MQ=255)
                                                                                                     ggCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTcc                                        >  1:56743/1‑149 (MQ=255)
                                                                                                     ggCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTcc                                        <  4:28161/149‑1 (MQ=255)
                                                                                                      gCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCt                                       >  4:596225/1‑149 (MQ=255)
                                                                                                            cAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCgg                                 <  2:257725/149‑1 (MQ=255)
                                                                                                             aGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGc                                <  1:17433/149‑1 (MQ=255)
                                                                                                              gCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCt                               >  1:343211/1‑149 (MQ=255)
                                                                                                                 ctctCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTtgc                            <  4:21328/149‑1 (MQ=255)
                                                                                                                   ctcCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTtgctg                          <  4:646561/149‑1 (MQ=255)
                                                                                                                        ggTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAATACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGtt                     <  3:60509/149‑1 (MQ=255)
                                                                                                                        ggTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGt                                          >  2:574440/1‑128 (MQ=255)
                                                                                                                        ggTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGt                                          <  1:574440/128‑1 (MQ=255)
                                                                                                                            tCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACt                 >  4:44124/1‑149 (MQ=255)
                                                                                                                              gAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACtgt               >  4:530552/1‑149 (MQ=255)
                                                                                                                                aaTTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGc             >  3:196651/1‑149 (MQ=255)
                                                                                                                                   aaaaggCAAACTGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTATTGACTGTGCTCg          >  4:588424/5‑149 (MQ=255)
                                                                                                                                    aacGGCAAACTGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCgg         >  2:281107/3‑149 (MQ=255)
                                                                                                                                      cGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGc                                                  >  2:161352/1‑106 (MQ=255)
                                                                                                                                      cGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGc                                                  <  1:161352/106‑1 (MQ=255)
                                                                                                                                        gCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTc     >  4:322965/1‑149 (MQ=255)
                                                                                                                                           aaCAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGAt  <  2:568947/149‑1 (MQ=255)
                                                                                                                                           aaCAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGAt  <  1:508280/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GGATGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCGTGAACGCCTTGTCCGGCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGTAGCCTGATGGAAATTTTCCTTAGATCGAGTCTCCTGCATGTTAAAGCGCGTGTTCCTCAGCCTGTTAGTCCTGATCGGCTTGCTGCTGTTGACTGTGCTCGGCCTCGAT  >  NC_000913/2232662‑2232949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: