Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,270,605 | G→A | 9.7% | intergenic (+60/‑121) | mepS → / → rtn | murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,270,605 | 0 | G | A | 9.7% | 185.3 / 9.3 | 62 | intergenic (+60/‑121) | mepS/rtn | murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
Reads supporting (aligned to +/- strand): ref base G (27/29); new base A (2/4); total (29/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 |
GTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTT > NC_000913/2270463‑2270749 | gtggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTg > 3:54736/1‑149 (MQ=255) ggtgTTTTTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCt > 2:338996/1‑149 (MQ=255) ggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCt < 1:10127/149‑1 (MQ=255) ggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCt < 2:349887/149‑1 (MQ=255) ggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCt < 4:68697/149‑1 (MQ=255) gtgtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtt > 2:566488/1‑149 (MQ=255) gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtttt < 1:210932/149‑1 (MQ=255) ttattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGt > 4:607946/1‑149 (MQ=255) tattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGtt < 1:338996/149‑1 (MQ=255) tttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCt > 3:669810/1‑139 (MQ=255) tttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGATAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCt < 4:669810/139‑1 (MQ=255) gCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttcattca > 3:662707/1‑149 (MQ=255) atgaatgaACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc > 2:519742/1‑149 (MQ=255) tgaatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatca < 1:418346/149‑1 (MQ=255) tgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAgaga > 4:184439/1‑149 (MQ=255) aCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGaaaa < 3:402110/149‑1 (MQ=255) ccGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAat < 1:481035/149‑1 (MQ=255) tACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAatgat < 4:54736/149‑1 (MQ=255) tACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAatgat < 1:397143/149‑1 (MQ=255) tGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtt > 4:141867/1‑117 (MQ=255) tGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtt < 3:141867/117‑1 (MQ=255) gaagaaGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGtttt > 4:21298/1‑124 (MQ=255) gaagaaGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGtttt < 3:21298/124‑1 (MQ=255) gaagaaGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatca > 4:271677/1‑133 (MQ=255) gaagaaGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatca < 3:271677/133‑1 (MQ=255) aagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcgcg > 4:77871/1‑149 (MQ=255) ttACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTg > 3:382081/1‑149 (MQ=255) gCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtttttt > 1:666313/1‑100 (MQ=255) gCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtttttt < 2:666313/100‑1 (MQ=255) gCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCta > 3:185809/1‑149 (MQ=255) cGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtttttt < 1:373751/97‑1 (MQ=255) cGCCGGGGTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtttttt > 2:373751/1‑97 (MQ=255) ccGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTc > 4:422577/1‑149 (MQ=255) tcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCAtt < 3:607946/149‑1 (MQ=255) gCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGtt < 4:662707/149‑1 (MQ=255) aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgt > 3:402247/1‑149 (MQ=255) aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgt > 2:443523/1‑149 (MQ=255) aCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAat < 3:359361/92‑1 (MQ=255) aCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAat > 4:359361/1‑92 (MQ=255) aCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgtt < 3:363590/149‑1 (MQ=255) aCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgtt < 1:519742/149‑1 (MQ=255) gTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTc < 1:566488/149‑1 (MQ=255) gTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTc < 4:442657/149‑1 (MQ=255) ggATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTaa < 3:184439/149‑1 (MQ=255) cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCa < 3:422577/149‑1 (MQ=255) aaTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTc < 1:52925/139‑1 (MQ=255) aaTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTc > 2:52925/1‑139 (MQ=255) aaTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg > 4:666091/1‑149 (MQ=255) aTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAgg > 2:269961/1‑149 (MQ=255) ccTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGaaa < 4:406753/149‑1 (MQ=255) gCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAAc < 4:382081/149‑1 (MQ=255) aTCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGa < 3:80936/149‑1 (MQ=255) cTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATc < 4:402247/149‑1 (MQ=255) gACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCAt > 3:151675/1‑149 (MQ=255) cTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgc > 4:81319/1‑149 (MQ=255) aGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTcc > 2:18501/1‑149 (MQ=255) cTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg < 2:188454/123‑1 (MQ=255) cTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg > 1:188454/1‑123 (MQ=255) tGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt < 4:185809/149‑1 (MQ=255) cTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt < 3:81319/149‑1 (MQ=255) cTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt > 2:248827/1‑149 (MQ=255) cTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt > 1:272914/1‑149 (MQ=255) | GTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTT > NC_000913/2270463‑2270749 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |