Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,290,450 C→T 9.2% intergenic (‑270/‑50) yejO ← / → narP pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,290,4500CT 9.2% 164.7 / 5.8 54intergenic (‑270/‑50)yejO/narPpseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ
Reads supporting (aligned to +/- strand):  ref base C (23/26);  new base T (3/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

TTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAA  >  NC_000913/2290305‑2290598
                                                                                                                                                 |                                                                                                                                                    
tttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAac                                                                                                                                                   <  2:641321/149‑1 (MQ=255)
       ttgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctacca                                                                                                                                            >  4:99710/1‑147 (MQ=255)
        tgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctaccag                                                                                                                                           <  3:99710/149‑4 (MQ=255)
         gttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCaa                                                                                                                                                    <  2:188346/139‑1 (MQ=255)
         gttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCaa                                                                                                                                                    >  1:188346/1‑139 (MQ=255)
         gttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTc                                                                                                                                          <  3:338413/149‑1 (MQ=255)
               cTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACAt                                                                                                                                    >  1:266402/1‑149 (MQ=255)
                 tgatgaGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATaa                                                                                                                                  <  3:118120/149‑1 (MQ=255)
                 tgatgaGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATaa                                                                                                                                  <  1:35332/149‑1 (MQ=255)
                          gCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATTTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCtaa                                                                                                                         >  1:182652/1‑149 (MQ=255)
                          gCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCtaa                                                                                                                         <  1:319398/149‑1 (MQ=255)
                           cTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCtaat                                                                                                                        <  1:591642/149‑1 (MQ=255)
                                    aaTTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCa                                                                                                               <  1:322999/149‑1 (MQ=255)
                                          gTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGac                                                                                                         >  3:624049/1‑149 (MQ=255)
                                              ttATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctacc                                                                                                                                             >  2:352129/1‑108 (MQ=255)
                                              ttATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctacc                                                                                                                                             <  1:352129/109‑2 (MQ=255)
                                                    tGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCt                                                                                               >  1:109132/1‑149 (MQ=255)
                                                     gAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTg                                                                                              >  4:329253/1‑149 (MQ=255)
                                                       aaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGaa                                                                                            <  4:624049/149‑1 (MQ=255)
                                                       aaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGaa                                                                                            <  2:266402/149‑1 (MQ=255)
                                                                      gAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAg                                                                             <  4:171437/149‑1 (MQ=255)
                                                                       aaCGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAgg                                                                            >  4:24404/1‑149 (MQ=255)
                                                                          gACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtga                                                                         >  1:177436/1‑149 (MQ=255)
                                                                                 ttGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATtgt                                                                  <  3:24404/149‑1 (MQ=255)
                                                                                       ttttCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatga                                                            <  1:377710/149‑1 (MQ=255)
                                                                                           cACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGatcat                                                        >  1:146216/1‑149 (MQ=255)
                                                                                            aCTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGatcatc                                                       >  3:313192/1‑149 (MQ=255)
                                                                                               aCATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCAc                                                    <  2:146216/149‑1 (MQ=255)
                                                                                                 aTGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCAcaa                                                  <  3:607083/149‑3 (MQ=255)
                                                                                                 aTGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACtt                                                  >  2:671869/1‑149 (MQ=255)
                                                                                                    tCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAgg                                                                            <  2:535681/120‑1 (MQ=255)
                                                                                                    tCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAgg                                                                            >  1:535681/1‑120 (MQ=255)
                                                                                                      catacataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCg                                             >  3:64194/1‑149 (MQ=255)
                                                                                                         acataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCGCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGAcg                                          >  4:239285/1‑149 (MQ=255)
                                                                                                          cataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGCTTCTAATAAAACCTCAGGAGTCTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGAcgc                                         >  1:615386/1‑149 (MQ=255)
                                                                                                           ataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGAcgcg                                        >  1:544574/1‑149 (MQ=255)
                                                                                                               aaTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGgtgt                                    >  2:490498/1‑149 (MQ=255)
                                                                                                                aTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGtt                                   <  2:177436/149‑1 (MQ=255)
                                                                                                                 tGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTc                                  <  4:430412/149‑1 (MQ=255)
                                                                                                                 tGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTc                                  <  3:49968/149‑1 (MQ=255)
                                                                                                                 tGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAACGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTc                                  <  1:490498/149‑1 (MQ=255)
                                                                                                                    ggTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTc                               <  2:544574/149‑1 (MQ=255)
                                                                                                                     gTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTc                               >  1:458925/1‑148 (MQ=255)
                                                                                                                     gTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTc                               <  2:458925/148‑1 (MQ=255)
                                                                                                                        aCATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGtt                           <  1:671869/149‑1 (MQ=255)
                                                                                                                          aTTCACGCGCCTGGTAGCGTTACCAACTCTACGCTCAAACATAATGCTTCTAATAAAACCTCAGTAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatga                                                            >  1:517415/1‑114 (MQ=255)
                                                                                                                          aTTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatga                                                            <  2:517415/114‑1 (MQ=255)
                                                                                                                                gcgcCTGGTAGGGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGATGCGGTGTTCGTCAGTTACTGGAGc                   >  3:456040/1‑149 (MQ=255)
                                                                                                                                    cTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGa               >  1:626062/1‑149 (MQ=255)
                                                                                                                                      ggTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATc             >  4:622092/1‑149 (MQ=255)
                                                                                                                                      cgTAGCGTTGGTAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATc             >  2:96810/2‑149 (MQ=255)
                                                                                                                                        tAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCt           <  4:191600/149‑1 (MQ=255)
                                                                                                                                         aGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTg          <  2:109132/149‑1 (MQ=255)
                                                                                                                                            gTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGct       >  4:201423/1‑149 (MQ=255)
                                                                                                                                                ccAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGGCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGa   >  1:445429/1‑149 (MQ=255)
                                                                                                                                                 cAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGaa  <  4:64194/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAA  >  NC_000913/2290305‑2290598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: