Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,391,417 C→G 7.1% W30S (TGG→TCG)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,391,4170CG 7.1% 347.8 / 15.2 99W30S (TGG→TCG) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (43/49);  new base G (5/2);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01

Only 100 of 102 total aligned reads displayed.

CAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGCCAA  >  NC_000913/2391269‑2391564
                                                                                                                                                    |                                                                                                                                                   
cAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAATCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGcc                                                                                                                                                     >  4:605452/1‑149 (MQ=255)
cAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGcc                                                                                                                                                     >  2:484560/1‑149 (MQ=255)
    ccGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGc                                                                                                                                                 >  1:211587/1‑149 (MQ=255)
        tgtAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtc                                                                                                                                             <  1:464646/149‑2 (MQ=255)
          tAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGga                                                                                                                                           <  3:634479/149‑1 (MQ=255)
          tAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGga                                                                                                                                           >  2:522877/1‑149 (MQ=255)
           aaaGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAGCCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgag                                                                                                                                          >  3:469553/1‑149 (MQ=255)
           aaaGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgag                                                                                                                                          >  3:519729/1‑149 (MQ=255)
            aaGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgaga                                                                                                                                         <  4:660103/149‑1 (MQ=255)
            aaGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgaga                                                                                                                                         <  4:336730/149‑1 (MQ=255)
              gCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGc                                                                                                                                       >  1:16240/1‑149 (MQ=255)
                  ggCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATca                                                                                                                                   <  4:238670/149‑1 (MQ=255)
                    cGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATcaca                                                                                                                                 >  4:530456/1‑149 (MQ=255)
                          cATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccacc                                                                                                                           >  4:353208/1‑149 (MQ=255)
                           aTCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccacca                                                                                                                          >  1:140829/1‑149 (MQ=255)
                            tCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccaccac                                                                                                                         >  2:276035/1‑149 (MQ=255)
                               aCGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGt                                                                                                                      <  3:542059/149‑1 (MQ=255)
                                cgcgCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTc                                                                                                                     <  1:33254/149‑1 (MQ=255)
                                 gcgcATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGACCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCa                                                                                                                    >  1:199546/1‑149 (MQ=255)
                                  cgcATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCaa                                                                                                                   <  1:298653/149‑1 (MQ=255)
                                        aGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgac                                                                                                             >  3:73593/1‑149 (MQ=255)
                                         gcggcgTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgacg                                                                                                            >  4:332974/1‑149 (MQ=255)
                                           ggcgTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGAt                                                                                                          <  3:304739/149‑1 (MQ=255)
                                            gcgTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATc                                                                                                         <  3:133975/149‑1 (MQ=255)
                                                  cGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAAc                                                                                                   <  2:208951/149‑1 (MQ=255)
                                                   gTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACg                                                                                                  >  1:540752/1‑149 (MQ=255)
                                                      cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTa                                                                                               >  2:434619/1‑149 (MQ=255)
                                                      cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTa                                                                                               <  3:652259/149‑1 (MQ=255)
                                                      cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTa                                                                                               >  3:381754/1‑149 (MQ=255)
                                                        tAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGc                                                                                             >  4:260306/1‑149 (MQ=255)
                                                            gcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTg                                                                                         <  1:434619/149‑1 (MQ=255)
                                                             cccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGc                                                                                        <  4:381754/149‑1 (MQ=255)
                                                                gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGCAGCAGTGCGAt                                                                                     <  3:605452/149‑1 (MQ=255)
                                                                 ccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTGGCAGTGCGATc                                                                                    <  3:404116/149‑1 (MQ=255)
                                                                      ccAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtt                                                                               <  4:350048/149‑1 (MQ=255)
                                                                      ccAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtt                                                                               <  4:211480/149‑1 (MQ=255)
                                                                        aacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtttt                                                                             >  1:657958/1‑149 (MQ=255)
                                                                        aacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtttt                                                                             >  2:34395/1‑149 (MQ=255)
                                                                         acaaACCAGAGGGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtg                                                                            >  3:392318/1‑149 (MQ=255)
                                                                         acaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtg                                                                            <  3:530456/149‑1 (MQ=255)
                                                                          caaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtgt                                                                           <  1:614839/149‑1 (MQ=255)
                                                                           aaaCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtgtg                                                                          >  2:262203/1‑149 (MQ=255)
                                                                            aaCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTgg                                                                         >  4:454005/1‑149 (MQ=255)
                                                                             aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGa                                                                        <  2:482190/149‑1 (MQ=255)
                                                                             aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGa                                                                        <  1:654141/149‑1 (MQ=255)
                                                                             aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGa                                                                        <  1:340525/149‑1 (MQ=255)
                                                                             aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTAGa                                                                        <  2:657958/149‑1 (MQ=255)
                                                                              ccAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAg                                                                       <  4:158759/149‑1 (MQ=255)
                                                                              ccAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAg                                                                       <  3:260306/149‑1 (MQ=255)
                                                                                agagCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGtt                                                                     >  2:274004/1‑149 (MQ=255)
                                                                                   gCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAtt                                                                  >  1:16107/1‑149 (MQ=255)
                                                                                   gCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTCAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAtt                                                                  <  1:202992/149‑1 (MQ=255)
                                                                                         ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATg                                                            <  2:145023/149‑1 (MQ=255)
                                                                                         ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATg                                                            <  3:353208/149‑1 (MQ=255)
                                                                                            gcgcCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCg                                                         <  4:519729/149‑1 (MQ=255)
                                                                                             cgccgcGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGa                                                        <  2:140829/149‑1 (MQ=255)
                                                                                                   gTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACg                                                  <  2:16107/149‑1 (MQ=255)
                                                                                                     tAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGc                                                <  2:16240/149‑1 (MQ=255)
                                                                                                       aGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCt                                              >  1:674406/1‑149 (MQ=255)
                                                                                                        gCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCtt                                             >  4:368049/1‑149 (MQ=255)
                                                                                                        gCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCtt                                             <  4:392318/149‑1 (MQ=255)
                                                                                                        gCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCATCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAt                                                                   <  1:655054/127‑1 (MQ=255)
                                                                                                        gCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCATCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAt                                                                   >  2:655054/1‑127 (MQ=255)
                                                                                                           cAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGc                                                                                                      >  2:453323/1‑89 (MQ=255)
                                                                                                           cAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGc                                                                                                      <  1:453323/89‑1 (MQ=255)
                                                                                                            aaTAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgtag                                         >  3:504765/1‑149 (MQ=255)
                                                                                                            aaTAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTATGGAGTTATTGTCATGGCGATTTACGGCCTTgtag                                         >  3:139198/1‑149 (MQ=255)
                                                                                                              tAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgtagta                                       <  3:405698/149‑1 (MQ=255)
                                                                                                              tAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgtagta                                       <  1:262203/149‑1 (MQ=255)
                                                                                                                 ccGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATc                                                                                                         <  2:66144/80‑1 (MQ=255)
                                                                                                                 ccGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATc                                                                                                         >  1:66144/1‑80 (MQ=255)
                                                                                                                  cGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTaa                                   >  1:271731/1‑149 (MQ=255)
                                                                                                                   gagaGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAAc                                  >  3:251624/1‑149 (MQ=255)
                                                                                                                          tagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTa                           >  2:513427/1‑149 (MQ=255)
                                                                                                                             cgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACa                        >  2:383387/1‑149 (MQ=255)
                                                                                                                              gtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACaa                       >  1:116238/1‑149 (MQ=255)
                                                                                                                               ttGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACg                                                  <  3:676877/121‑1 (MQ=255)
                                                                                                                               ttGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACg                                                  <  3:48261/121‑1 (MQ=255)
                                                                                                                               ttGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACg                                                  >  4:676877/1‑121 (MQ=255)
                                                                                                                               ttGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACg                                                  >  4:48261/1‑121 (MQ=255)
                                                                                                                               ttGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACaaa                      <  3:99786/149‑1 (MQ=255)
                                                                                                                                tGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAAc                     <  2:674406/149‑1 (MQ=255)
                                                                                                                                   ggAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACaaa                      >  3:274876/1‑145 (MQ=255)
                                                                                                                                   ggAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACaaa                      <  4:274876/145‑1 (MQ=255)
                                                                                                                                    gAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAAt                             <  1:678620/137‑1 (MQ=255)
                                                                                                                                     tctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCActg                >  1:269530/6‑149 (MQ=255)
                                                                                                                                     aaaTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAtt                                                                  >  4:6922/1‑99 (MQ=255)
                                                                                                                                     aaaTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTAtt                                                                  <  3:6922/99‑1 (MQ=255)
                                                                                                                                       tccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgta                                          <  3:137299/118‑1 (MQ=255)
                                                                                                                                       tccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTgta                                          >  4:137299/4‑121 (MQ=255)
                                                                                                                                       tccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCActgct              >  1:590745/4‑149 (MQ=255)
                                                                                                                                       tccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCActgct              >  1:108979/4‑149 (MQ=255)
                                                                                                                                         gATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTgg            >  2:138631/1‑149 (MQ=255)
                                                                                                                                         caTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTgg            >  3:16657/2‑149 (MQ=255)
                                                                                                                                           ttGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGat          >  1:389666/1‑149 (MQ=255)
                                                                                                                                            tGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGata         <  1:383387/149‑1 (MQ=255)
                                                                                                                                                tCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGc     <  1:484560/149‑1 (MQ=255)
                                                                                                                                                tCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGc     <  3:153441/149‑1 (MQ=255)
                                                                                                                                                tCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGc     <  4:617184/149‑1 (MQ=255)
                                                                                                                                                   ccACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGCCaa  <  3:368049/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACGGCCTTGTAGTAGTAACGGAATTAACAAACCACTGCTGGATATTGCCAA  >  NC_000913/2391269‑2391564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: