Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,405,941 C→G 6.6% G234A (GGC→GCC)  lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,405,9410CG 6.6% 374.9 / 12.6 106G234A (GGC→GCC) lrhAtranscriptional repressor of flagellar, motility and chemotaxis genes
Reads supporting (aligned to +/- strand):  ref base C (56/42);  new base G (5/2);  total (62/44)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

Only 100 of 112 total aligned reads displayed.

CGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTC  >  NC_000913/2405793‑2406089
                                                                                                                                                    |                                                                                                                                                    
cGCCTGATAGATCACCTGGGCCAGCTCAGTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGc                                                                                                                                                      <  4:443372/149‑1 (MQ=255)
 gCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGc                                                                                                                                                      <  4:294382/148‑1 (MQ=255)
 gCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGc                                                                                                                                                      >  3:294382/1‑148 (MQ=255)
   cTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCaa                                                                                                                                                   <  2:620073/149‑1 (MQ=255)
      aTAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGAc                                                                                                                                                <  1:513567/149‑1 (MQ=255)
      aTAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGAc                                                                                                                                                >  4:409830/1‑149 (MQ=255)
       tAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGAcc                                                                                                                                               <  3:381928/149‑1 (MQ=255)
        aGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCg                                                                                                                                              >  2:661429/1‑149 (MQ=255)
          aTCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGc                                                                                                                                            >  3:689432/1‑149 (MQ=255)
           tCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCt                                                                                                                                           >  4:659830/1‑149 (MQ=255)
           tCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCt                                                                                                                                           >  1:298886/1‑149 (MQ=255)
             aCCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCttt                                                                                                                                         >  1:16983/1‑149 (MQ=255)
                         tCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACg                                                                                                                             >  3:416852/1‑149 (MQ=255)
                         tCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACg                                                                                                                             >  3:502283/1‑149 (MQ=255)
                               ttaCTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGc                                                                                                                       >  4:600798/1‑149 (MQ=255)
                                   tGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCttt                                                                                                                                         >  2:130050/1‑127 (MQ=255)
                                   tGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCttt                                                                                                                                         <  1:130050/127‑1 (MQ=255)
                                   tGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGa                                                                                                                   >  3:442715/1‑149 (MQ=255)
                                   tGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGa                                                                                                                   <  2:43368/149‑1 (MQ=255)
                                     gACGGATCATAGCACAGCAAATATTAGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAg                                                                                                                 >  2:540866/1‑149 (MQ=255)
                                         gATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTc                                                                                                             <  3:164510/149‑1 (MQ=255)
                                          aTCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCg                                                                                                            >  3:173441/1‑149 (MQ=255)
                                          aTCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCg                                                                                                            >  2:40071/1‑149 (MQ=255)
                                           tCATAGCACAGCAAATACTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGa                                                                                                           <  4:103412/149‑1 (MQ=255)
                                              tAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGc                                                                                                        <  1:381932/149‑1 (MQ=255)
                                                     gCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATaa                                                                                                 >  4:181830/1‑149 (MQ=255)
                                                      cAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAg                                                                                                >  2:52219/1‑149 (MQ=255)
                                                        aaTATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGcc                                                                                              >  3:172468/1‑149 (MQ=255)
                                                            ttCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTc                                                                                          <  4:689432/149‑1 (MQ=255)
                                                            ttCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTc                                                                                          <  1:661429/149‑1 (MQ=255)
                                                            ttCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTc                                                                                          >  3:138561/1‑149 (MQ=255)
                                                                     aGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGaa                                                                                 >  4:25607/1‑149 (MQ=255)
                                                                         aGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATc                                                                             <  3:78608/149‑1 (MQ=255)
                                                                          gcggcgGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCt                                                                            <  1:540866/149‑1 (MQ=255)
                                                                          gcggcgGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCt                                                                            >  2:477871/1‑149 (MQ=255)
                                                                            ggcggTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGc                                                                          <  2:298886/149‑1 (MQ=255)
                                                                            ggcggTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGc                                                                          <  4:502283/149‑1 (MQ=255)
                                                                                 tAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttat                                                                     >  1:109052/1‑149 (MQ=255)
                                                                                    gCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTaa                                                                  <  1:477871/149‑1 (MQ=255)
                                                                                     ccATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAg                                                                 >  2:163261/1‑149 (MQ=255)
                                                                                     ccATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAg                                                                 >  1:560925/1‑149 (MQ=255)
                                                                                      cATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGc                                                                >  2:286442/1‑149 (MQ=255)
                                                                                      cATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGc                                                                <  1:163261/149‑1 (MQ=255)
                                                                                       aTCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCg                                                               >  3:151501/1‑149 (MQ=255)
                                                                                        tCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGt                                                              >  4:214116/1‑149 (MQ=255)
                                                                                          tACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCg                                                            >  4:41145/1‑149 (MQ=255)
                                                                                            cTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGt                                                                                                              <  4:569682/97‑1 (MQ=255)
                                                                                            cTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGt                                                                                                              >  3:569682/1‑97 (MQ=255)
                                                                                            cTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGcc                                                          >  2:629954/1‑149 (MQ=255)
                                                                                            cTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGcc                                                          <  4:617183/149‑1 (MQ=255)
                                                                                             tCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCa                                                         >  4:689098/1‑149 (MQ=255)
                                                                                             tCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTACTTTATTAAGCGTCGCCa                                                         <  3:232097/149‑1 (MQ=255)
                                                                                                aCTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTtta                                                                      <  4:476138/133‑1 (MQ=255)
                                                                                                aCTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTtta                                                                      >  3:476138/1‑133 (MQ=255)
                                                                                                aCTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCa                                                      >  2:258880/1‑149 (MQ=255)
                                                                                                aCTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCa                                                      <  4:213821/149‑1 (MQ=255)
                                                                                                    aGCACACGCAGGTCCGGCCTCATCATCTCAGCCGGTCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGCACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCAGCTTTATTAAGCGTCGCCAGCACCat                                                  >  4:578766/1‑149 (MQ=255)
                                                                                                     gCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCata                                                 >  2:572190/1‑149 (MQ=255)
                                                                                                       ccacGCAGGTCCGGGCTCATCTTCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATc                                               <  4:650706/148‑1 (MQ=255)
                                                                                                       acacGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATc                                               >  1:654575/1‑149 (MQ=255)
                                                                                                         acGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATcgc                                             <  4:442715/149‑1 (MQ=255)
                                                                                                              ggTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAAc                                        >  1:211417/1‑149 (MQ=255)
                                                                                                                tCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACgg                                      <  3:659830/149‑1 (MQ=255)
                                                                                                                  cGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGc                                    <  4:138561/149‑1 (MQ=255)
                                                                                                                    ggCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTa                                  <  3:76650/149‑1 (MQ=255)
                                                                                                                      cTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAgg                                >  2:283983/1‑149 (MQ=255)
                                                                                                                         atcatcTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGatc                             >  2:420100/1‑149 (MQ=255)
                                                                                                                           catcTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGatcat                           <  1:436724/149‑1 (MQ=255)
                                                                                                                             tgaaagccGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATcc                         >  2:317849/7‑149 (MQ=255)
                                                                                                                             tctcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGa                                                                                                      >  3:20515/1‑72 (MQ=255)
                                                                                                                             tctcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGa                                                                                                      <  4:20515/72‑1 (MQ=255)
                                                                                                                             tctcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATcc                         >  4:393227/1‑149 (MQ=255)
                                                                                                                              ctcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCa                        <  3:41145/149‑1 (MQ=255)
                                                                                                                               tcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAg                       <  3:654475/149‑1 (MQ=255)
                                                                                                                               tcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAg                       >  3:49906/1‑149 (MQ=255)
                                                                                                                               tcAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGACGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAg                       >  4:502369/1‑149 (MQ=255)
                                                                                                                                    cGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATa                  >  3:154202/1‑149 (MQ=255)
                                                                                                                                    cGGTCTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATa                  >  1:269068/1‑149 (MQ=255)
                                                                                                                                    cGGTCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCAc                                                     >  1:33016/1‑114 (MQ=255)
                                                                                                                                    cGGTCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCAc                                                     <  2:33016/114‑1 (MQ=255)
                                                                                                                                       tcTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGc                                                                          <  4:275716/89‑1 (MQ=255)
                                                                                                                                       tcTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGc                                                                          >  3:275716/2‑90 (MQ=255)
                                                                                                                                       tcTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTa                                  >  4:106401/2‑130 (MQ=255)
                                                                                                                                       tcTTGGCGTGACGGCAAGGCCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTa                                  <  3:106401/129‑1 (MQ=255)
                                                                                                                                       tcTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACaa               >  2:110939/2‑149 (MQ=255)
                                                                                                                                        cTTGCCGTGACGACAAGACCGGCTTTCACTGCCGCACGAACGGCCGAAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACagg              >  2:551766/1‑147 (MQ=255)
                                                                                                                                         ttGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAaga             <  2:221871/149‑1 (MQ=255)
                                                                                                                                         ttGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAaga             >  1:24954/1‑149 (MQ=255)
                                                                                                                                         ttGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAaga             <  1:605020/149‑1 (MQ=255)
                                                                                                                                         ttGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAaga             <  1:72122/149‑1 (MQ=255)
                                                                                                                                          tGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAagag            <  3:25607/149‑1 (MQ=255)
                                                                                                                                            ccGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAggg          >  1:329547/1‑149 (MQ=255)
                                                                                                                                              gTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGAt        <  2:308178/149‑1 (MQ=255)
                                                                                                                                               tCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATc       >  4:223227/1‑149 (MQ=255)
                                                                                                                                                 aCGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCgg     <  3:181830/149‑1 (MQ=255)
                                                                                                                                                   gCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGTAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGatcatc                          >  2:590301/1‑126 (MQ=255)
                                                                                                                                                   gCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGTAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGatcatc                          <  1:590301/126‑1 (MQ=255)
                                                                                                                                                   gCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCt   >  3:438379/1‑149 (MQ=255)
                                                                                                                                                    ccAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTc  <  1:258880/149‑1 (MQ=255)
                                                                                                                                                    ccAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGACGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTc  <  4:173441/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTC  >  NC_000913/2405793‑2406089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: