Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,566,496 A→G 8.0% I130T (ATC→ACC)  eutA ← reactivating factor for ethanolamine ammonia lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,566,4960AG 8.0% 192.4 / 6.1 62I130T (ATC→ACC) eutAreactivating factor for ethanolamine ammonia lyase
Reads supporting (aligned to +/- strand):  ref base A (30/27);  new base G (3/2);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

GACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTCCGGCTCAA  >  NC_000913/2566350‑2566643
                                                                                                                                                  |                                                                                                                                                   
gACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATc                                                                                                                                                   <  4:254735/149‑1 (MQ=255)
gACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATc                                                                                                                                                   <  3:351674/149‑1 (MQ=255)
 accaccGACGTTGAGGCAGGCCGTACCATTGCTTTTTCCGTCAGCGAACACTGCGTAGTTCGCGCTGCCACCGCCGATGTCGGTTTTCAGTACCAAACAACGCCGATGTGCATTTCGGGTTTGTGTTATGGCTCCGTGACCTCCGATCa                                                                                                                                                  >  4:366624/1‑149 (MQ=255)
     ccGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGa                                                                                                                                              >  1:111338/1‑149 (MQ=255)
     ccGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGa                                                                                                                                              <  4:645061/149‑1 (MQ=255)
      cGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGAt                                                                                                                                             >  3:168999/1‑149 (MQ=255)
          gTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGa                                                                                                                                         >  2:370351/1‑149 (MQ=255)
                gcaggcagTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCg                                                                                                                                   >  1:474898/1‑149 (MQ=255)
                    gcagTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGccc                                                                                                                               <  2:65184/149‑1 (MQ=255)
                       gTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGgc                                                                                                                            <  2:276688/149‑1 (MQ=255)
                              tGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCa                                                                                                                     >  4:361561/1‑149 (MQ=255)
                                aTTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAAc                                                                                                                   <  3:276297/149‑1 (MQ=255)
                                           aTCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGTGCTGGCAACGACAAAATCGc                                                                                                        >  1:371414/1‑149 (MQ=255)
                                              gAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGATCGCCGGTCACGGa                                                                                                                                              >  2:518821/1‑108 (MQ=255)
                                              gAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGATCGCCGGTCACGGa                                                                                                                                              <  1:518821/108‑1 (MQ=255)
                                              gAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCa                                                                                                     <  2:111338/149‑1 (MQ=255)
                                                aCAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGc                                                                                                   <  4:21222/149‑1 (MQ=255)
                                                aCAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGc                                                                                                   >  1:634538/1‑149 (MQ=255)
                                                  aGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGa                                                                                                 <  2:256983/149‑1 (MQ=255)
                                                  aGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGa                                                                                                 >  1:467414/1‑149 (MQ=255)
                                                   gggCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGAc                                                                                                >  2:166941/1‑149 (MQ=255)
                                                      cGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTga                                                                                             <  1:462913/149‑1 (MQ=255)
                                                      cGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTga                                                                                             <  4:168999/149‑1 (MQ=255)
                                                       gTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTgag                                                                                            <  2:634538/149‑1 (MQ=255)
                                                          gTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTgagaga                                                                                         >  3:660288/1‑149 (MQ=255)
                                                          gTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTgagaga                                                                                         <  3:384939/149‑1 (MQ=255)
                                                          gTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGCTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTgagaga                                                                                         <  4:575144/149‑1 (MQ=255)
                                                                 gTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGc                                                                                                                                    <  4:306908/99‑1 (MQ=255)
                                                                 gTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGc                                                                                                                                    >  3:306908/1‑99 (MQ=255)
                                                                     cACCGCCGATGTCGATATTCAGTACCCGACACAGCCCTTGTTCAGAAAGGGTTTGTGCGCCGGCTCCGTGACCGGCGATCACGGATTGGAGGTGCGGCCCGGCGCTGGCAACGACATAATCGCCCAGCGACTGAGAGAGCGCCATCAcc                                                                              >  3:581985/1‑149 (MQ=255)
                                                                        cgccgATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCAccgcc                                                                           <  2:419008/149‑1 (MQ=255)
                                                                            gATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGc                                                                       <  1:370351/149‑1 (MQ=255)
                                                                               gTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAg                                                                    >  3:178459/1‑149 (MQ=255)
                                                                                tCGATATTCAGTACCCGACACAGCCGTTGTTCTGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGc                                                                   >  1:329495/1‑149 (MQ=255)
                                                                                 caatatTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCa                                                                  >  3:441418/3‑149 (MQ=255)
                                                                                  gATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACt                                                                                               >  2:430978/1‑119 (MQ=255)
                                                                                  gATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACt                                                                                               <  1:430978/119‑1 (MQ=255)
                                                                                  gATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCAt                                                                 >  1:184214/1‑149 (MQ=255)
                                                                                     atTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTTGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGc                                                              >  3:235393/1‑149 (MQ=255)
                                                                                      ttCAGTACCCCACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGCGAGAGCGCCATCACCGCCGGGCGAGCATTGCg                                                             <  4:441418/149‑1 (MQ=255)
                                                                                       tCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGATCGCCGGTCACGGa                                                                                                                                              >  1:501932/1‑67 (MQ=38)
                                                                                       tCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGATCGCCGGTCACGGa                                                                                                                                              <  2:501932/67‑1 (MQ=38)
                                                                                            aCCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGtttt                                                       >  4:166627/1‑149 (MQ=255)
                                                                                                 acacAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCgcgc                                                  <  2:189696/149‑1 (MQ=255)
                                                                                                  cacaGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCt                                                 <  2:329495/149‑1 (MQ=255)
                                                                                                  cacaGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCACTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCt                                                 <  2:474898/149‑1 (MQ=255)
                                                                                                                 gAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtgatgat                                  >  2:32218/1‑149 (MQ=255)
                                                                                                                  aaaGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtgatgatg                                 >  1:368475/1‑149 (MQ=255)
                                                                                                                   aaGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtgatgatga                                >  4:278047/1‑149 (MQ=255)
                                                                                                                      ggTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGATAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATgg                             <  4:658214/149‑1 (MQ=255)
                                                                                                                       gTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtgatgat                                  >  1:34197/1‑143 (MQ=255)
                                                                                                                       gTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtgatgat                                  <  2:34197/143‑1 (MQ=255)
                                                                                                                             gCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGa                      >  1:492425/1‑149 (MQ=255)
                                                                                                                                ccGGCTGCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATc                   >  2:491096/1‑149 (MQ=255)
                                                                                                                                  ggCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCaa                 <  2:467414/149‑1 (MQ=255)
                                                                                                                                   gCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAAc                <  1:166941/149‑1 (MQ=255)
                                                                                                                                     tCCGTGATCGCCGGTCACGGATTCGAGGTGCGGCCCGGCGCTGGCAAGGACAAAATCGCCCAGCGACGGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGc              >  4:668736/1‑149 (MQ=255)
                                                                                                                                     tCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGc              >  1:524748/1‑149 (MQ=255)
                                                                                                                                         tGACCGGCGATCACGGATTCGAGGTGCGGCCCGGTGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTc          <  2:371414/149‑1 (MQ=255)
                                                                                                                                         tGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTc          >  2:515083/1‑149 (MQ=255)
                                                                                                                                           aCCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTCCg        >  4:334932/1‑149 (MQ=255)
                                                                                                                                                 gATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTCCGGCTCaa  >  2:28483/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTCCGGCTCAA  >  NC_000913/2566350‑2566643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑