Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,675,531 C→T 5.5% V77V (GTG→GTA)  yphC ← putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,675,5310CT 5.5% 240.6 / 2.7 72V77V (GTG→GTA) yphCputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (35/33);  new base T (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGATCAGTACCTGG  >  NC_000913/2675392‑2675678
                                                                                                                                           |                                                                                                                                                   
aGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGc                                                                                                                                            >  3:93409/1‑149 (MQ=255)
 gCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGcc                                                                                                                                           >  3:411455/1‑149 (MQ=255)
    gTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGc                                                                                                                                        <  3:191081/149‑1 (MQ=255)
        gCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACgg                                                                                                                                    >  4:189833/1‑149 (MQ=255)
         ccGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGt                                                                                                                                   <  4:93409/149‑1 (MQ=255)
            cTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATTGCCACAATCTGCCCGCACGGTTCa                                                                                                                                >  4:662080/1‑149 (MQ=255)
            cTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCa                                                                                                                                >  1:521261/1‑149 (MQ=255)
                  cTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCTACAATCTGCCCGCACGGTTCATGACCg                                                                                                                          >  3:475907/1‑149 (MQ=255)
                  cTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCTACAATCTGCCCGCACGGTTCATGACCg                                                                                                                          >  1:640798/1‑149 (MQ=255)
                   ttCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGt                                                                                                                         >  1:200912/1‑149 (MQ=255)
                   ttCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGt                                                                                                                         <  3:189833/149‑1 (MQ=255)
                     cGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTg                                                                                                                       <  3:47218/149‑1 (MQ=255)
                          gTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATaaa                                                                                                                  <  4:180154/149‑1 (MQ=255)
                          gTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATaaa                                                                                                                  <  1:503497/149‑1 (MQ=255)
                          gTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATaaa                                                                                                                  <  3:681265/149‑1 (MQ=255)
                             cAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGtt                                                                                                                        <  4:109820/140‑1 (MQ=255)
                             cAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGtt                                                                                                                        <  2:254898/140‑1 (MQ=255)
                             cAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGtt                                                                                                                        >  3:109820/1‑140 (MQ=255)
                             cAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGtt                                                                                                                        >  1:254898/1‑140 (MQ=255)
                               aGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAACCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCt                                                                                                             <  3:626719/149‑1 (MQ=255)
                                  aaTAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTc                                                                                                                                 >  1:302450/1‑126 (MQ=255)
                                  aaTAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTc                                                                                                                                 <  2:302450/126‑1 (MQ=255)
                                       gAAAACCGCGACGGCAGTTCGTGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAAc                                                                                                     >  2:356450/1‑149 (MQ=255)
                                        aaaaCCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACg                                                                                                    >  1:626580/1‑149 (MQ=255)
                                          aaCCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGt                                                                                                  <  2:261768/149‑1 (MQ=255)
                                                 aCGGCAGTTCGTGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCgg                                                                                           <  1:356450/149‑1 (MQ=255)
                                                      aGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTgcc                                                                                      >  3:256927/1‑149 (MQ=255)
                                                        ttCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTgccgc                                                                                    >  3:3428/1‑149 (MQ=255)
                                                        ttCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTgccgc                                                                                    >  1:40929/1‑149 (MQ=255)
                                                               aaaaaCCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCtgtg                                                                             >  2:36360/1‑149 (MQ=255)
                                                               aaaaaCCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCtgtg                                                                             <  3:256440/149‑1 (MQ=255)
                                                                      acaGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGgt                                                                      <  1:59128/149‑1 (MQ=255)
                                                                      acaGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGgt                                                                      >  2:59128/1‑149 (MQ=255)
                                                                        aGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGgtgt                                                                    >  2:409281/1‑149 (MQ=255)
                                                                         gCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCGCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGtt                                                                   <  2:400666/149‑1 (MQ=255)
                                                                           cAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTtga                                                                 <  2:378275/149‑1 (MQ=255)
                                                                           cAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTtga                                                                 <  3:527454/149‑1 (MQ=255)
                                                                           cAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTtga                                                                 <  2:40929/149‑1 (MQ=255)
                                                                            aGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTtgat                                                                <  4:411455/149‑1 (MQ=255)
                                                                             gAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTtgatg                                                               >  2:350426/1‑149 (MQ=255)
                                                                               aaTGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATTGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTgatgat                                                             <  3:662080/149‑1 (MQ=255)
                                                                                aTGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTCATAAAGCCCTGGTATAACGGGTTATCGGGTGCCGCCGCTGTTGCACGGTGTTGATgata                                                            >  1:188593/1‑149 (MQ=255)
                                                                                 tgtgATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATgatag                                                           >  2:660445/1‑149 (MQ=255)
                                                                                   tgATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATgatagat                                                         >  3:402749/1‑149 (MQ=255)
                                                                                       acacCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTtga                                                                 >  1:144146/1‑137 (MQ=255)
                                                                                       acacCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTtga                                                                 <  2:144146/137‑1 (MQ=255)
                                                                                            aGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTAt                                                                                              >  3:401124/1‑103 (MQ=255)
                                                                                            aGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCAAGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTAt                                                                                              <  4:401124/103‑1 (MQ=255)
                                                                                              cACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACAt                                              >  1:163813/1‑149 (MQ=255)
                                                                                                   ggTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGTCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCtt                                         <  1:290469/149‑1 (MQ=255)
                                                                                                          ctctTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCaaa                                  >  4:276208/1‑149 (MQ=255)
                                                                                                              ttAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTtgatga                                                              >  1:417710/1‑117 (MQ=255)
                                                                                                              ttAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTtgatga                                                              <  2:417710/117‑1 (MQ=255)
                                                                                                                 aaaTGGCGGCAGCCTTGCCCCATCGCTACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCgga                           <  4:475907/149‑1 (MQ=255)
                                                                                                                 aaaTGGCGGCAGCCTTGCCCCATCGCTACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCgga                           <  2:640798/149‑1 (MQ=255)
                                                                                                                  aaTGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCggag                          >  3:13149/1‑149 (MQ=255)
                                                                                                                        ggcAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTc                    <  1:36360/149‑1 (MQ=255)
                                                                                                                             ccTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCAtttt               >  1:199408/1‑149 (MQ=255)
                                                                                                                               ttGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGTTATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGa             >  1:474693/1‑149 (MQ=255)
                                                                                                                                   cccATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCggagga                        <  3:165421/134‑1 (MQ=255)
                                                                                                                                   cccATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCggagga                        >  4:165421/1‑134 (MQ=255)
                                                                                                                                   cccATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGAtt                      >  1:455192/1‑136 (MQ=255)
                                                                                                                                   cccATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGAtt                      <  2:455192/136‑1 (MQ=255)
                                                                                                                                   cccATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGAt            >  3:59899/1‑146 (MQ=255)
                                                                                                                                   cccATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGAt            <  4:59899/146‑1 (MQ=255)
                                                                                                                                   cccATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGATCAg         >  4:177090/1‑149 (MQ=255)
                                                                                                                                    ccATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTGCCGCAAATCCCGGAGGATTTCATTTTGATCAGt        >  1:290988/1‑149 (MQ=255)
                                                                                                                                     cATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGATCACTa       <  2:474693/149‑1 (MQ=255)
                                                                                                                                        cGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGATCAGTACCt    <  3:648956/149‑1 (MQ=255)
                                                                                                                                        cGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGATCAGTACCt    <  4:3428/149‑1 (MQ=255)
                                                                                                                                        cGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGATCAGTACCt    <  3:81553/149‑1 (MQ=255)
                                                                                                                                         gCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGATCAGTACCTg   >  1:228794/1‑149 (MQ=255)
                                                                                                                                          ccACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTg                                                   >  2:500802/1‑100 (MQ=255)
                                                                                                                                          ccACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTg                                                   <  1:500802/100‑1 (MQ=255)
                                                                                                                                          ccACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGATCAGTACCTgg  >  4:141879/1‑149 (MQ=255)
                                                                                                                                          ccACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGATCAGTACCTgg  >  3:428489/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
AGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGCAAATCCCGGAGGATTTCATTTTGATCAGTACCTGG  >  NC_000913/2675392‑2675678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑