Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,691,169 A→T 10.9% noncoding (172/184 nt) glmY ← sRNA activator of glmS mRNA, glmZ processing antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,691,1690AT10.9% 193.1 / 15.0 64noncoding (172/184 nt)glmYsRNA activator of glmS mRNA, glmZ processing antagonist
Reads supporting (aligned to +/- strand):  ref base A (30/27);  new base T (4/3);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

AGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGG  >  NC_000913/2691022‑2691312
                                                                                                                                                    |                                                                                                                                               
aGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑aa                                                                                                                                                <  1:267337/149‑1 (MQ=255)
      agcagAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTAccc                                                                                                                                          >  4:625029/1‑149 (MQ=255)
       gcagAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCg                                                                                                                                         >  2:686062/1‑149 (MQ=255)
          gAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCt                                                                                                                                      <  1:239181/149‑1 (MQ=255)
           aaTCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑TAATTCCCGGCtt                                                                                                                                     <  4:252702/149‑1 (MQ=255)
           aaTCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCtt                                                                                                                                     <  4:186326/149‑1 (MQ=255)
            aTCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATT‑CCCGGCttt                                                                                                                                    <  2:163717/149‑1 (MQ=255)
             tCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGAGAAAAACAGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCGTGATGCTAACCGAGGGGAAGTTCAGGTACAACAAAGCCGGG‑AATCACCCGGCTTTg                                                                                                                                   >  4:427379/1‑149 (MQ=255)
                  aaaaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATg                                                                                                                              >  4:531887/1‑149 (MQ=255)
                      aTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAAt                                                                                                                          >  4:347619/1‑149 (MQ=255)
                            gCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCg                                                                                                                    >  4:406422/1‑149 (MQ=255)
                               ttACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑TAATTCCCGGCTTTGtt                                                                                                                                 >  1:229952/1‑133 (MQ=255)
                               ttACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑TAATTCCCGGCTTTGtt                                                                                                                                 <  2:229952/133‑1 (MQ=255)
                               ttACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTg                                                                                                                 >  3:620736/1‑149 (MQ=255)
                                tACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGc                                                                                                                <  3:190599/149‑1 (MQ=255)
                                 aCCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTAt                                                                                                                               >  3:355868/1‑133 (MQ=255)
                                 aCCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTAt                                                                                                                               <  4:355868/133‑1 (MQ=255)
                                     gTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGc                                                                                                                >  4:641510/1‑144 (MQ=255)
                                     gTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGc                                                                                                                <  3:641510/144‑1 (MQ=255)
                                         tCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGa                                                                                                       >  2:300713/1‑149 (MQ=255)
                                          cGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGAc                                                                                                      <  3:531887/149‑1 (MQ=255)
                                           gTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACg                                                                                                     >  4:660842/1‑149 (MQ=255)
                                            tAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑TAATTCCCGGCtt                                                                                                                                     >  4:561592/1‑116 (MQ=255)
                                            tAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑TAATTCCCGGCtt                                                                                                                                     <  3:561592/116‑1 (MQ=255)
                                            tAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTAt                                                                                                                               >  3:324126/1‑122 (MQ=255)
                                            tAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTAt                                                                                                                               <  4:324126/122‑1 (MQ=255)
                                             aaTGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGtt                                                                                                   >  3:340360/1‑149 (MQ=255)
                                                gAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCg                                                                                                >  3:358888/1‑149 (MQ=255)
                                                          aaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGtt                                                                                      <  3:625029/149‑1 (MQ=255)
                                                            aCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGa                                                                                    <  1:300713/149‑1 (MQ=255)
                                                                   aGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCt                                                                             >  1:596445/1‑149 (MQ=255)
                                                                     cGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTAc                                                                           >  3:495571/1‑149 (MQ=255)
                                                                           aaGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGat                                                                     <  3:646803/149‑1 (MQ=255)
                                                                           aaGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGat                                                                     <  4:340360/149‑1 (MQ=255)
                                                                           aaGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGat                                                                     >  1:252859/1‑149 (MQ=255)
                                                                                  tACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGaa                                                              <  2:596445/149‑1 (MQ=255)
                                                                                  tACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGaa                                                              <  3:498916/149‑1 (MQ=255)
                                                                                     tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                           >  1:185867/1‑149 (MQ=255)
                                                                                     tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                           >  1:570693/1‑149 (MQ=255)
                                                                                     tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                           >  4:243206/1‑149 (MQ=255)
                                                                                     tctcGTCAAACGCGCATAGCCTGATGCTAACTGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCCGTTCTGTTATGGAATTAGGCGCTGCCTAACTCGAAGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                           <  3:427379/149‑1 (MQ=255)
                                                                                         gTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATAGAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGAc                                                       >  3:395589/1‑149 (MQ=255)
                                                                                            aGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggc                                                    <  2:630622/149‑1 (MQ=255)
                                                                                             gACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggca                                                   >  3:98973/1‑149 (MQ=255)
                                                                                                gcgAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTa                                                                            <  3:238508/121‑1 (MQ=255)
                                                                                                gcgAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTa                                                                            >  4:238508/1‑121 (MQ=255)
                                                                                                        ccTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                        <  3:347619/149‑1 (MQ=255)
                                                                                                        ccTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                        <  3:243206/149‑1 (MQ=255)
                                                                                                        ccTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                        <  1:686062/149‑1 (MQ=255)
                                                                                                        ccTGATGATAACCGAGGGGAAGTTCAGATACAACAAAGCCAGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                        <  3:73779/149‑1 (MQ=255)
                                                                                                          tGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTtgttgt                                      <  4:620736/149‑1 (MQ=255)
                                                                                                          tGATGCTAACCGAGGGGAAGTTCAGAGACAACAAAGCCAGG‑AATTACCCGGCTTTGTTATGGAATACGGCGGTGCCTAACTCGACGTTTCGCCCGATGCTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTtgttgt                                      >  3:429676/1‑149 (MQ=255)
                                                                                                            aTGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGc                                    <  1:515527/149‑1 (MQ=255)
                                                                                                               cTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTc                                 >  3:209008/1‑149 (MQ=255)
                                                                                                                 aaCCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCAt                               <  3:225901/149‑1 (MQ=255)
                                                                                                                    cGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCg                            <  2:570693/149‑1 (MQ=255)
                                                                                                                                        acaaAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTccc        >  3:575175/1‑149 (MQ=255)
                                                                                                                                        acaaAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTccc        >  1:162503/1‑149 (MQ=255)
                                                                                                                                         caaAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCg       >  3:681906/1‑149 (MQ=255)
                                                                                                                                          aaaGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGa      >  4:156995/1‑149 (MQ=255)
                                                                                                                                           aaGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGaa     >  4:330306/1‑149 (MQ=255)
                                                                                                                                           aaGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGaa     <  4:358888/149‑1 (MQ=255)
                                                                                                                                             gCCGGG‑TAATTCCCGGCTTTGTTGTGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAgg   >  3:1082/1‑149 (MQ=255)
                                                                                                                                             gCCGGG‑TAATTCCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAgg   >  1:615355/1‑149 (MQ=255)
                                                                                                                                              ccGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAggg  <  4:681906/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
AGGGGCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGG  >  NC_000913/2691022‑2691312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑