| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,822,684 | A→G | 8.5% | intergenic (‑45/+24) | recX ← / ← recA | regulatory protein for RecA/DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,822,684 | 0 | A | G | 8.5% | 178.4 / 7.1 | 59 | intergenic (‑45/+24) | recX/recA | regulatory protein for RecA/DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor |
| Reads supporting (aligned to +/- strand): ref base A (23/31); new base G (3/2); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-02 | |||||||||||
GTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGT > NC_000913/2822540‑2822832 | gTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCt > 4:626741/1‑149 (MQ=255) gCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGACGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtgt < 2:11846/149‑1 (MQ=255) tCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATc > 2:282347/1‑149 (MQ=255) tGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCaaa < 4:250389/149‑1 (MQ=255) tGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCaaa < 2:555167/149‑1 (MQ=255) tCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACaa > 3:246840/1‑149 (MQ=255) tCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACaa > 2:229213/1‑149 (MQ=255) cTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGAc < 1:680558/149‑1 (MQ=255) gtgATCGCGCACCGCCAGAATGCGTACTGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGa < 3:475737/149‑1 (MQ=255) gtgATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGa < 1:173951/149‑1 (MQ=255) cgcgCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTaaa < 1:177952/149‑1 (MQ=255) aCCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCt > 1:24951/1‑149 (MQ=255) cGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTc < 1:536575/149‑1 (MQ=255) cGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTc < 2:108076/149‑1 (MQ=255) cGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTc < 4:246840/149‑1 (MQ=255) gCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCttttgctt > 2:166812/1‑118 (MQ=255) gCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCTtttgctt < 1:166812/124‑4 (MQ=255) gCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCg > 2:2120/1‑149 (MQ=255) ccAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAGAAATCTTCGt < 2:497670/149‑1 (MQ=255) ccAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGt < 3:51502/149‑1 (MQ=255) gAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAg > 4:281010/1‑149 (MQ=255) tGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATTTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGttt < 2:514436/149‑1 (MQ=255) gCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTc > 2:357453/1‑149 (MQ=255) gCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTc < 4:197745/149‑1 (MQ=255) tACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGc > 4:204205/1‑149 (MQ=255) cACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGc > 4:168426/1‑149 (MQ=255) cGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCt > 3:422840/1‑149 (MQ=255) atatGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACaga < 4:104943/149‑1 (MQ=255) tatGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTACGCTATCATCTACagag > 4:135727/1‑149 (MQ=255) cGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTa < 4:385317/82‑1 (MQ=255) cGGGCGACGGGATGTTGATCCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTa > 3:385317/1‑82 (MQ=255) cGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGc < 1:357453/149‑1 (MQ=255) cGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGc < 4:422840/149‑1 (MQ=255) ttaTTCTGTCATGTCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCgg < 2:24951/147‑1 (MQ=255) tGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCgg > 1:276047/1‑149 (MQ=255) tGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCgg < 2:327748/149‑1 (MQ=255) ttCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGtt > 4:30701/1‑149 (MQ=255) ttCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGtt > 4:452440/1‑149 (MQ=255) ttCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGtt < 1:229213/149‑1 (MQ=255) tGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagca > 3:388489/1‑149 (MQ=255) cATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAgcagca > 1:90383/1‑149 (MQ=255) atatCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCagcagcga < 2:276047/149‑3 (MQ=255) atatCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTACGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCaa < 3:135727/149‑1 (MQ=255) tatCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAAc < 1:424982/149‑1 (MQ=255) cTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGGTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGCGTTCGGGTTGCTCAGCAGCAACTCAc > 4:20276/1‑149 (MQ=255) ttACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACg > 1:555399/1‑149 (MQ=255) cAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTAc < 2:90383/149‑1 (MQ=255) cTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACttt < 3:452440/149‑1 (MQ=255) ttAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttc > 3:23444/1‑149 (MQ=255) aaaaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttcttc < 3:20276/149‑1 (MQ=255) aaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTtctc < 4:23444/149‑1 (MQ=255) aaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTtctc < 1:282347/149‑1 (MQ=255) aGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGa < 3:281010/149‑1 (MQ=255) acaaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtct > 1:544708/3‑149 (MQ=255) aaaaGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtct > 4:404241/1‑149 (MQ=255) tctGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagca < 3:111316/108‑1 (MQ=255) tctGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagca > 4:111316/3‑110 (MQ=255) cGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCgg < 3:626741/149‑1 (MQ=255) gACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCggg > 4:372131/1‑149 (MQ=255) aCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGt > 3:441197/1‑149 (MQ=255) | GTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGT > NC_000913/2822540‑2822832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |