| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,258,251 | T→A | 8.5% | intergenic (‑241/+34) | yhaO ← / ← tdcG | putative transporter/L‑serine dehydratase 3, anaerobic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,258,251 | 0 | T | A | 8.5% | 201.6 / 8.5 | 59 | intergenic (‑241/+34) | yhaO/tdcG | putative transporter/L‑serine dehydratase 3, anaerobic |
| Reads supporting (aligned to +/- strand): ref base T (30/24); new base A (3/2); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01 | |||||||||||
ATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAG > NC_000913/3258105‑3258389 | aTAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACAttt < 4:660656/149‑1 (MQ=255)aTAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACAttt < 1:572533/149‑1 (MQ=255) aaGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTtgtg > 1:581197/1‑149 (MQ=255) gAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCa > 4:547154/1‑149 (MQ=255) gAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCa > 4:268530/1‑149 (MQ=255) aTACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAg > 1:603871/1‑149 (MQ=255) aCGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGa < 4:18880/149‑1 (MQ=255) aCGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGa < 2:390347/149‑1 (MQ=255) cgcgcgAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGAt < 3:268530/149‑1 (MQ=255) agagAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACa < 2:603871/149‑1 (MQ=255) ttCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAg > 2:266572/1‑149 (MQ=255) acaATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCg > 2:498972/1‑149 (MQ=255) aaTCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCa > 2:575373/1‑149 (MQ=255) tCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGa > 4:151393/1‑149 (MQ=255) aaGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGAcc < 2:581197/149‑1 (MQ=255) aGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCa > 2:44820/1‑149 (MQ=255) aaaTAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATg < 3:151393/149‑1 (MQ=255) agaTGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGtcct > 2:681718/1‑149 (MQ=255) aTGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGtcctcc > 3:287638/1‑149 (MQ=255) aTAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGt < 2:624101/149‑1 (MQ=255) gAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATg > 2:301876/1‑149 (MQ=255) gAATTTTCTACGAAATTAATCACAGGAGAATATTCATTCTCTATTTGCTGCAGATCACAAAAAAAAGGTGCACATTTGTTCTCCCAAAGATGAACGCTGACAACAATGTCAGCAGAAAACCACTTTAATGGACAGTCCTCCGCGTGagg > 2:340986/1‑147 (MQ=255) actaAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGt > 4:551634/1‑149 (MQ=255) ctaAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGtcctc > 1:632150/1‑130 (MQ=255) ctaAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGtcctc < 2:632150/130‑1 (MQ=255) ctaAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTa > 3:567642/1‑149 (MQ=255) taAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGc < 1:159568/121‑1 (MQ=255) taAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGc > 2:159568/1‑121 (MQ=255) taAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTAt < 1:498972/149‑1 (MQ=255) taAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTAt > 2:218014/1‑149 (MQ=255) aaTTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTa < 3:243765/146‑1 (MQ=255) aaTTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTa > 4:243765/1‑146 (MQ=255) tCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGtt < 4:567642/149‑1 (MQ=255) cGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCATCCGCGTGATGTTTCGCGGTATTTATCGTTc > 1:197580/1‑149 (MQ=255) gaATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCtt < 1:44820/149‑1 (MQ=255) tatTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGc > 1:428088/1‑149 (MQ=255) ttcattcTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGa < 4:483540/58‑1 (MQ=255) ttcattcTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGa > 3:483540/1‑58 (MQ=255) tctATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCAt < 3:6038/149‑1 (MQ=255) tATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATAc > 3:356893/1‑149 (MQ=255) tttGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACAt > 1:42247/1‑149 (MQ=255) ttGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCAGTATTTATCGTTCATATCTTTGCCGGTTTCATACATc < 3:551634/149‑1 (MQ=255) gCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGtctc < 1:575373/149‑1 (MQ=255) tCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTc > 2:354693/1‑115 (MQ=255) tCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTc < 1:354693/115‑1 (MQ=255) acaAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACt < 1:266572/149‑1 (MQ=255) acaAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACt > 4:276926/1‑149 (MQ=255) aaaaaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGtctc > 3:254665/1‑140 (MQ=255) aaaaaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGtctc < 4:254665/140‑1 (MQ=255) aaaaaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACttt > 1:240548/1‑149 (MQ=255) aaaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTAt < 1:681718/149‑1 (MQ=255) ccttgggTGCACAAATGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACttt < 3:154083/141‑1 (MQ=255) ccttgggTGCACAAATGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACttt > 4:154083/6‑146 (MQ=255) ccttgggTGCACAAATGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATc > 1:466116/6‑149 (MQ=255) aaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATc < 1:301876/149‑1 (MQ=255) aaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCTGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCg > 1:512235/1‑149 (MQ=255) ttgggTGCACAAATGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCa < 2:116914/68‑1 (MQ=38) ttgggTGCACAAATGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCa > 1:116914/4‑71 (MQ=38) aaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAg < 3:276926/149‑1 (MQ=255) gTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAg < 2:66112/146‑1 (MQ=255) gTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAg > 1:66112/1‑146 (MQ=255) | ATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAG > NC_000913/3258105‑3258389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |