Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,303,611 T→A 9.2% I55N (ATT→AAT)  yhbW → putative luciferase‑like monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,303,6110TA 9.2% 326.3 / 18.1 98I55N (ATT→AAT) yhbWputative luciferase‑like monooxygenase
Reads supporting (aligned to +/- strand):  ref base T (47/42);  new base A (4/5);  total (51/47)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 101 total aligned reads displayed.

CGTTTTCGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGG  >  NC_000913/3303467‑3303759
                                                                                                                                                |                                                                                                                                                    
cGTTTTCGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGcc                                                                                                                                                  <  4:141208/149‑1 (MQ=255)
 gTTTTCGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCAtt                                                                                                                                                     <  1:520153/145‑1 (MQ=255)
 gTTTTCGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCAtt                                                                                                                                                     >  2:520153/1‑145 (MQ=255)
 gTTTTCGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCa                                                                                                                                                 <  4:133393/149‑1 (MQ=255)
  ttctCGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAg                                                                                                                                                >  1:343047/4‑149 (MQ=255)
    ttCGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCATTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCAATGCCAGtc                                                                                                                                              <  4:622361/149‑2 (MQ=255)
    ttCGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCAATGCCAGtc                                                                                                                                              <  2:499878/149‑2 (MQ=255)
     tCGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGGAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCAATGCCAGtca                                                                                                                                             <  4:154588/149‑3 (MQ=255)
      cGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGtgct                                                                                                                                            <  3:74340/149‑1 (MQ=255)
       gCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGtgctg                                                                                                                                           <  4:604771/149‑1 (MQ=255)
       gCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGtgctg                                                                                                                                           >  4:493994/1‑149 (MQ=255)
         tACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGcc                                                                                                                                         >  3:538287/1‑149 (MQ=255)
           cTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCAc                                                                                                                                       >  4:486585/1‑149 (MQ=255)
               aTCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCg                                                                                                                                   >  2:377884/1‑149 (MQ=255)
                tCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCgg                                                                                                                                  <  3:377229/149‑1 (MQ=255)
                  tGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACGCCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTa                                                                                                                                <  4:400258/149‑1 (MQ=255)
                   ggCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTAt                                                                                                                               <  2:90187/149‑1 (MQ=255)
                        ccATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATc                                                                                                                          >  2:443869/1‑149 (MQ=255)
                        ccATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATc                                                                                                                          <  4:275200/149‑1 (MQ=255)
                         cATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCg                                                                                                                         >  2:156354/1‑149 (MQ=255)
                          aTTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCgg                                                                                                                        >  4:150427/1‑149 (MQ=255)
                          aTTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCgg                                                                                                                        <  1:113817/149‑1 (MQ=255)
                          aTTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCgg                                                                                                                        >  4:567290/1‑149 (MQ=255)
                              ccGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTAt                                                                                                                    >  3:536044/1‑149 (MQ=255)
                               cGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGTTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATc                                                                                                                   >  1:618317/1‑149 (MQ=255)
                                gAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCt                                                                                                                  >  2:288685/1‑149 (MQ=255)
                                  aGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGAGCTCCCCCGTCTGGCTGAAAAGCGCGGCGATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTgg                                                                                                                >  2:304675/1‑149 (MQ=255)
                                   ggTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTggc                                                                                                               <  1:672492/149‑1 (MQ=255)
                                        ttcAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTa                                                                                                                                >  2:162022/1‑127 (MQ=255)
                                        ttcAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTa                                                                                                                                <  1:162022/127‑1 (MQ=255)
                                        ttcAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGa                                                                                                          >  1:390662/1‑149 (MQ=255)
                                        ttcAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGa                                                                                                          >  2:566178/1‑149 (MQ=255)
                                         tcAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGaa                                                                                                         <  2:288591/149‑1 (MQ=255)
                                           aGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATa                                                                                                       <  1:443869/149‑1 (MQ=255)
                                            gcgcGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATac                                                                                                      >  2:660514/1‑149 (MQ=255)
                                              gcgAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATacca                                                                                                    >  4:144723/1‑149 (MQ=255)
                                              gcgAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATacca                                                                                                    <  2:390662/149‑1 (MQ=255)
                                                gagaAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTa                                                                                                                                >  4:165713/1‑119 (MQ=255)
                                                gagaAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTa                                                                                                                                <  3:165713/119‑1 (MQ=255)
                                                     gCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTACCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCt                                                                                             >  2:561544/1‑149 (MQ=255)
                                                       aTTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGc                                                                                           >  3:102323/1‑149 (MQ=255)
                                                           tcCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCt                                                                                       >  1:408604/1‑149 (MQ=255)
                                                              cACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTggg                                                                                    <  1:283031/149‑1 (MQ=255)
                                                               aCTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTgggg                                                                                   <  2:178105/149‑1 (MQ=255)
                                                                 tctctcGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTc                                                                                 <  3:567290/149‑1 (MQ=255)
                                                                  ctctcGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTACCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCt                                                                                <  1:561544/149‑1 (MQ=255)
                                                                   tctcGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTg                                                                               >  4:300255/1‑149 (MQ=255)
                                                                    ctcGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTgg                                                                              >  2:204254/1‑149 (MQ=255)
                                                                    ctcGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCAATGCCAGtcata                                                                                                                                           >  4:662226/1‑84 (MQ=255)
                                                                    ctcGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCAATGCCAGtcata                                                                                                                                           <  3:662226/88‑5 (MQ=255)
                                                                       gATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTggcgg                                                                           >  4:671904/1‑149 (MQ=255)
                                                                        aTCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTggcggc                                                                          <  2:343047/149‑1 (MQ=255)
                                                                         tctcGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGaa                                                                                                         >  4:1063/1‑117 (MQ=255)
                                                                         tctcGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGaa                                                                                                         <  3:1063/117‑1 (MQ=255)
                                                                         tctcGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGgcggcg                                                                         <  3:671904/149‑1 (MQ=255)
                                                                          ctcGCCCGTCTGTCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGt                                                                        <  4:170091/149‑1 (MQ=255)
                                                                            cGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGa                                                                      <  3:493994/149‑1 (MQ=255)
                                                                            cGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGa                                                                      <  1:156354/149‑1 (MQ=255)
                                                                             gCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGAt                                                                     <  4:135017/149‑1 (MQ=255)
                                                                                 gTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTg                                                                 <  3:150427/149‑1 (MQ=255)
                                                                                  tCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGc                                                                <  3:3415/149‑1 (MQ=255)
                                                                                  tCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGc                                                                <  1:288685/149‑1 (MQ=255)
                                                                                    tGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCt                                                              <  3:206742/149‑1 (MQ=255)
                                                                                      gCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTaa                                                            >  1:550117/1‑149 (MQ=255)
                                                                                       cTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAAc                                                           <  4:129048/149‑1 (MQ=255)
                                                                                           aaaGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACt                                                       >  4:201483/1‑149 (MQ=255)
                                                                                            aaGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTc                                                      >  1:207887/1‑149 (MQ=255)
                                                                                              gcgcGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCAc                                                    >  1:76645/1‑149 (MQ=255)
                                                                                                   gCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTg                                               <  3:300255/149‑1 (MQ=255)
                                                                                                   gCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTg                                               <  3:342256/149‑1 (MQ=255)
                                                                                                         atcGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCAtt                                         >  3:133463/1‑149 (MQ=255)
                                                                                                         atcGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCAtt                                         >  2:514305/1‑149 (MQ=255)
                                                                                                         atcGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCAtt                                         >  1:585703/1‑149 (MQ=255)
                                                                                                         atcGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCAtt                                         >  4:255107/1‑149 (MQ=255)
                                                                                                         atcGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCAtt                                         >  3:385398/1‑149 (MQ=255)
                                                                                                          tcGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTg                                        >  3:439308/1‑149 (MQ=255)
                                                                                                            gCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCa                                      >  4:485673/1‑149 (MQ=255)
                                                                                                               aCTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGaa                                   <  3:255107/149‑1 (MQ=255)
                                                                                                                         aGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCCAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCa                         <  1:566178/149‑1 (MQ=255)
                                                                                                                         aGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCAACACGCTGCATCTGGGGTCTGGAGGCGTGATGTTGCCTAATCACTCTCCGTTGGTCATTGCAGAACAGTTCGGCa                         <  1:304675/149‑1 (MQ=255)
                                                                                                                          gAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCAc                        >  1:638327/1‑149 (MQ=255)
                                                                                                                             caccacAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCt                     >  2:109448/1‑149 (MQ=255)
                                                                                                                              accacAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                    <  3:46341/149‑1 (MQ=255)
                                                                                                                                cacaATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTaa                  <  3:107792/149‑1 (MQ=255)
                                                                                                                                 acaATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACAACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAAt                 <  4:555310/149‑1 (MQ=255)
                                                                                                                                   aaTATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATac               >  3:263969/1‑149 (MQ=255)
                                                                                                                                    atatGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATaca              <  3:144723/149‑1 (MQ=255)
                                                                                                                                      agcaCTGGCAATGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACa                                 >  3:245753/4‑128 (MQ=255)
                                                                                                                                      agcaCTGGCAATGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACa                                 <  4:245753/125‑1 (MQ=255)
                                                                                                                                       gcaCTGGCAATGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACActc           >  3:557232/3‑149 (MQ=255)
                                                                                                                                       gcaCTGGCAATGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACActc           >  3:670678/3‑149 (MQ=255)
                                                                                                                                        gACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTggg                                                                                    >  2:464864/1‑75 (MQ=255)
                                                                                                                                        gACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTggg                                                                                    <  1:464864/75‑1 (MQ=255)
                                                                                                                                        gACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACActct          >  2:136483/1‑149 (MQ=255)
                                                                                                                                         aCTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTa         <  1:216937/149‑1 (MQ=255)
                                                                                                                                           tGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATc       >  2:459562/1‑149 (MQ=255)
                                                                                                                                           tGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATc       >  3:579045/1‑149 (MQ=255)
                                                                                                                                             gCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCg     >  1:498069/1‑149 (MQ=255)
                                                                                                                                               aTTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCggg   >  1:304635/1‑149 (MQ=255)
                                                                                                                                                ttGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCgggg  >  1:599512/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CGTTTTCGCTACTCGATCTGGCCCCCATTCCCGAAGGTTCTTCAGCGCGAGAAGCATTCTCCCACTCTCTCGATCTCGCCCGTCTGGCTGAAAAGCGCGGCTATCATCGCTACTGGCTGGCAGAACACCACAATATGACTGGCATTGCCAGTGCTGCCACGTCGGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGG  >  NC_000913/3303467‑3303759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: