Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 263,358 C→T 5.0% L104L (TTG→TTA ykfI ← CP4‑6 prophage; toxin of the YkfI‑YafW toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913263,3580CT 5.0% 336.4 / 4.5 99L104L (TTG→TTAykfICP4‑6 prophage; toxin of the YkfI‑YafW toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (50/44);  new base T (3/2);  total (53/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 104 total aligned reads displayed.

CATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAGGGTGATACCGG  >  NC_000913/263210‑263506
                                                                                                                                                    |                                                                                                                                                    
cATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGc                                                                                                                                                      >  4:417538/1‑149 (MQ=255)
 aTGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGca                                                                                                                                                     >  1:54229/1‑149 (MQ=255)
 aTGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGca                                                                                                                                                     >  3:324267/1‑149 (MQ=255)
  tGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGcaa                                                                                                                                                    >  3:426073/1‑149 (MQ=255)
         ttgtCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCa                                                                                                                                             <  3:514245/149‑1 (MQ=255)
            tCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGtt                                                                                                                                          >  4:139213/1‑149 (MQ=255)
            tCCGTAAAAAGATAACGCGACTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGtt                                                                                                                                          <  1:196408/149‑1 (MQ=255)
                    aaGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGtt                                                                                                                                          <  1:241545/141‑1 (MQ=255)
                    aaGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGtt                                                                                                                                          >  2:241545/1‑141 (MQ=255)
                           cgcgCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGc                                                                                                                           <  1:217414/149‑1 (MQ=255)
                            gcgcCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCa                                                                                                                          >  1:322171/1‑149 (MQ=255)
                              gcCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGa                                                                                                                        <  4:173302/149‑1 (MQ=255)
                               ccTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGaa                                                                                                                       >  4:662574/1‑149 (MQ=255)
                                cTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAAt                                                                                                                      >  1:328094/1‑149 (MQ=255)
                                cTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAAt                                                                                                                      >  4:675916/1‑149 (MQ=255)
                                cTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAAt                                                                                                                      <  2:322171/149‑1 (MQ=255)
                                cTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAAt                                                                                                                      >  4:516885/1‑149 (MQ=255)
                                    cGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGGAGTTCAGAAATGTAAATCGGGAAGGTTGTACGAAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCt                                                                                                                  >  2:204080/1‑149 (MQ=255)
                                    cGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCt                                                                                                                  <  2:481696/149‑1 (MQ=255)
                                    cGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCt                                                                                                                  <  2:353292/149‑1 (MQ=255)
                                           tagCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGccc                                                                                                           >  1:659198/1‑149 (MQ=255)
                                           tagCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGccc                                                                                                           >  3:667060/1‑149 (MQ=255)
                                                ggCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCACAGAATGTCTGCAGCCCGgaga                                                                                                      <  1:421299/149‑1 (MQ=255)
                                                   gcATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATaa                                                                                                   >  1:50825/1‑149 (MQ=255)
                                                     aTTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGccc                                                                                                           <  2:464952/139‑1 (MQ=255)
                                                     aTTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGccc                                                                                                           >  1:464952/1‑139 (MQ=255)
                                                      ttACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCt                                                                                                                  <  1:57066/131‑1 (MQ=255)
                                                      ttACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCt                                                                                                                  >  2:57066/1‑131 (MQ=255)
                                                          gCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGAtt                                                                                            >  1:662573/1‑149 (MQ=255)
                                                          gCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGAt                                                                                             <  4:215720/148‑1 (MQ=255)
                                                          gCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGAt                                                                                             >  3:215720/1‑148 (MQ=255)
                                                            aaTAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGt                                                                                          >  4:9138/1‑149 (MQ=255)
                                                            aaTAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGt                                                                                          >  1:168092/1‑149 (MQ=255)
                                                               aGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGga                                                                                                >  3:335556/1‑140 (MQ=255)
                                                               aGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGga                                                                                                <  4:335556/140‑1 (MQ=255)
                                                               aGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTcc                                                                                       >  1:455047/1‑149 (MQ=255)
                                                                ggTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCt                                                                                      <  3:516885/149‑1 (MQ=255)
                                                                  tAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCt                                                                                      <  4:215345/147‑1 (MQ=255)
                                                                  tAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCt                                                                                      >  3:215345/1‑147 (MQ=255)
                                                                  tAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGc                                                                                    >  3:285255/1‑149 (MQ=255)
                                                                  tAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGc                                                                                    <  2:455047/149‑1 (MQ=255)
                                                                   aaaCAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGgaga                                                                                              <  1:487153/138‑1 (MQ=255)
                                                                   aaaCAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGgaga                                                                                              >  2:487153/1‑138 (MQ=255)
                                                                    aaCAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCt                                                                                                                  <  1:138520/117‑1 (MQ=255)
                                                                    aaCAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCa                                                                                  >  3:119687/1‑149 (MQ=255)
                                                                     aCAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATAGTTCCTGCCAg                                                                                 <  4:63236/149‑1 (MQ=255)
                                                                       aaGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCt                                                                               <  2:54229/149‑1 (MQ=255)
                                                                       aaGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCt                                                                               >  3:544623/1‑149 (MQ=255)
                                                                           gaggaAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAAt                                                                           >  3:290031/1‑149 (MQ=255)
                                                                            aggaAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATc                                                                          <  2:504781/149‑1 (MQ=255)
                                                                                aGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCtt                                                                      >  2:560467/1‑149 (MQ=255)
                                                                                 gTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTc                                                                     <  1:818/149‑1 (MQ=255)
                                                                                 gTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTc                                                                     <  2:573555/149‑1 (MQ=255)
                                                                                  ttCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGAt                                                                                                     <  4:553491/116‑1 (MQ=255)
                                                                                  ttCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGAt                                                                                                     >  3:553491/1‑116 (MQ=255)
                                                                                   tCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCt                                                                   <  4:667060/149‑1 (MQ=255)
                                                                                   tCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCt                                                                   <  4:544623/149‑1 (MQ=255)
                                                                                         aTGTAAATGGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCt                                                                                      >  3:636597/1‑124 (MQ=255)
                                                                                         aTGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCt                                                                                      <  4:636597/124‑1 (MQ=255)
                                                                                                 cGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCa                                                     <  4:324267/149‑1 (MQ=255)
                                                                                                 cGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCa                                                     <  1:576285/149‑1 (MQ=255)
                                                                                                  gggAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAg                                                    >  1:53447/1‑149 (MQ=255)
                                                                                                   ggAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGc                                                   >  4:548252/1‑149 (MQ=255)
                                                                                                   ggAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTATTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGc                                                   >  1:630513/1‑149 (MQ=255)
                                                                                                    gAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCt                                                  >  3:76810/1‑149 (MQ=255)
                                                                                                     aaGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTc                                                 <  3:662574/149‑1 (MQ=255)
                                                                                                           gTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTAttt                                           >  4:267532/1‑149 (MQ=255)
                                                                                                             aCGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTAttttt                                         <  3:548252/149‑1 (MQ=255)
                                                                                                             aCGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTAttttt                                         <  2:168092/149‑1 (MQ=255)
                                                                                                              cGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTc                                        <  3:139213/149‑1 (MQ=255)
                                                                                                               gCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCt                                       >  4:515173/1‑149 (MQ=255)
                                                                                                               gCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCt                                       >  4:188481/1‑149 (MQ=255)
                                                                                                                    gTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAg                                  >  1:157567/1‑149 (MQ=255)
                                                                                                                     ttCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGa                                 <  4:426073/149‑1 (MQ=255)
                                                                                                                       cATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGaaa                               >  4:47992/1‑149 (MQ=255)
                                                                                                                        aTCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGTGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGaaaa                              >  2:539148/1‑149 (MQ=255)
                                                                                                                        aTCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTcc                                                                                       <  1:583845/92‑1 (MQ=255)
                                                                                                                        aTCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTcc                                                                                       >  2:583845/1‑92 (MQ=255)
                                                                                                                        aTCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGaaaa                              >  4:678274/1‑149 (MQ=255)
                                                                                                                        aTCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGaaaa                              >  2:141864/1‑149 (MQ=255)
                                                                                                                             actacGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCAc                         <  2:328094/149‑1 (MQ=255)
                                                                                                                               tacGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGt                                                                 >  2:75763/1‑107 (MQ=255)
                                                                                                                               tacGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGt                                                                 <  1:75763/107‑1 (MQ=255)
                                                                                                                               tacGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACgg                       <  3:417538/149‑1 (MQ=255)
                                                                                                                               tacGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACgg                       >  2:333630/1‑149 (MQ=255)
                                                                                                                                 gcttgttgCGGCAAAGCCGTAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCa                     >  4:492259/3‑149 (MQ=255)
                                                                                                                                   ttgttgCGGCAAAGCCGTAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCg                                                               >  4:451492/1‑105 (MQ=255)
                                                                                                                                   ttgttgCGGCAAAGCCGTAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCg                                                               <  3:451492/105‑1 (MQ=255)
                                                                                                                                   ttgttgCGGCAAAGCCGTAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGCACCAGCTCGTAttttt                                         >  4:52353/1‑127 (MQ=255)
                                                                                                                                   ttgttgCGGCAAAGCCGTAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTAttttt                                         <  3:52353/127‑1 (MQ=255)
                                                                                                                                     gttACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCt                                                                       >  4:402057/1‑95 (MQ=255)
                                                                                                                                     gttACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCt                                                                       <  3:402057/95‑1 (MQ=255)
                                                                                                                                      ttACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGc                >  3:396448/1‑149 (MQ=255)
                                                                                                                                         cGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAg             <  3:47992/149‑1 (MQ=255)
                                                                                                                                         cGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAg             <  1:333630/149‑1 (MQ=255)
                                                                                                                                          ggCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAgg            <  1:387997/149‑1 (MQ=255)
                                                                                                                                           gCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCCGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCATCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAggg           >  4:475847/1‑149 (MQ=255)
                                                                                                                                                  cGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAGGGTGATAcc    >  4:412439/1‑149 (MQ=255)
                                                                                                                                                   gCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAGGGTGATACCg   <  2:53447/149‑1 (MQ=255)
                                                                                                                                                    caacaaGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAGGGGGATACCgg  >  4:321469/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGTACGCAATGTTCATCGTACTACGTTGTTACGGCTTTGCCGCAACAAGCCAGTTGCCTGCCGCGCTCGCAGAATGTCTGCAGCCCGGAGATAAGGAGATTGTTCCTGCCAGCTAAATCCCTTCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAGGGTGATACCGG  >  NC_000913/263210‑263506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: