Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,492,430	C→T	7.9%	intergenic (‑133/‑138)	ppiA ← / → tsgA	peptidyl‑prolyl cis‑trans isomerase A (rotamase A)/putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,492,430	0	C	T	7.9%	190.0 / 4.4	61	intergenic (‑133/‑138)	ppiA/tsgA	peptidyl‑prolyl cis‑trans isomerase A (rotamase A)/putative transporter
Reads supporting (aligned to +/- strand):  ref base C (29/27);  new base T (3/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GGGTCGATTTGAACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCATTTTATTTTTGAACGCAGGCCAATCATGACTAACA  >  NC_000913/3492282‑3492577                                                                                                                                                     |                                                                                                                                                    gggTCGATTTGAACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACTTATCTGAAATATATGTAACCTAACGATCACTTTTTATTcc                                                                                                                                                     <  1:200470/149‑1 (MQ=255)      gATTTCCACAGGAGATTTACTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGAAATGCAGTTACTTAATTTTACATATCTGAAATATATGTAACCTGACGATCACTTTTTTTTCTTTGAt                                                                                                                                                >  4:560732/1‑149 (MQ=255)       aTTTGAACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATc                                                                                                                                               >  2:633044/1‑149 (MQ=255)           gAACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCaaaa                                                                                                                                           <  1:350951/149‑1 (MQ=255)                tGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCAcc                                                                                                                                      <  1:633044/149‑1 (MQ=255)                         cTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCTGTGATCAAAATCACCTCTTAAAAt                                                                                                                             >  3:145270/1‑149 (MQ=255)                         cTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCTGTGATCAAAATCACCTCTTAAAAt                                                                                                                             >  1:679371/1‑149 (MQ=255)                          ttAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATg                                                                                                                            >  4:217110/1‑149 (MQ=255)                               aaacaGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAAtt                                                                                                                       >  4:644220/1‑149 (MQ=255)                                aacaGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAAttt                                                                                                                      >  3:561809/1‑149 (MQ=255)                                 acaGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTa                                                                                                                     >  2:91633/1‑149 (MQ=255)                                  caGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAg                                                                                                                    <  3:35977/149‑1 (MQ=255)                                      gCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAAc                                                                                                                <  4:561809/149‑1 (MQ=255)                                         gAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCTGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGa                                                                                                             <  4:145270/149‑1 (MQ=255)                                         gAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCTGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGa                                                                                                             <  2:679371/149‑1 (MQ=255)                                            aaaaaGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAGAATGCaa                                                                                                                         >  1:593597/1‑134 (MQ=255)                                            aaaaaGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCaa                                                                                                                         <  2:593597/134‑1 (MQ=255)                                               aaGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCaa                                                                                                       <  3:154705/149‑1 (MQ=255)                                               aaGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCaa                                                                                                       <  1:164042/149‑1 (MQ=255)                                                   aaGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATaaa                                                                                                   >  4:149356/1‑149 (MQ=255)                                                       gAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACAt                                                                                               >  3:122941/1‑149 (MQ=255)                                                              ttCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACa                                                                                        >  4:387055/1‑149 (MQ=255)                                                                   aaTAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATca                                                                                   <  3:387055/149‑1 (MQ=255)                                                                     tAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATcaca                                                                                 >  3:79694/1‑149 (MQ=255)                                                                          ttCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGAtt                                                                                                           <  4:5074/118‑1 (MQ=255)                                                                          ttCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGAtt                                                                                                           >  3:5074/1‑118 (MQ=255)                                                                          ttCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACaaaa                                                                              >  1:23616/1‑147 (MQ=255)                                                                          ttCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACaaaa                                                                              <  2:23616/147‑1 (MQ=255)                                                                          ttCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATc                                                                            >  4:36226/1‑149 (MQ=255)                                                                             aaGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCAcc                                                                         <  1:109931/149‑1 (MQ=255)                                                                                  cAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTaaaa                                                                    <  1:27695/149‑1 (MQ=255)                                                                                  cAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTaaaa                                                                    <  3:36226/149‑1 (MQ=255)                                                                                  cAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTaaaa                                                                    <  4:79694/149‑1 (MQ=255)                                                                                    aGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATc                                                                  <  1:91633/149‑1 (MQ=255)                                                                                      gCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGc                                                                <  3:149356/149‑1 (MQ=255)                                                                                        cATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGccc                                                              <  2:287567/149‑1 (MQ=255)                                                                                            ccTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGGTTGCAATAAAACa                                                                                                >  3:291897/1‑111 (MQ=255)                                                                                            ccTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGGTTGCAATAAAACa                                                                                                <  4:291897/111‑1 (MQ=255)                                                                                             cTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTcg                                                         >  4:637028/1‑149 (MQ=255)                                                                                                                tCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGcc                                      >  3:37618/1‑149 (MQ=255)                                                                                                                tCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGcc                                      >  4:575078/1‑149 (MQ=255)                                                                                                                tCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGcc                                      >  2:277265/1‑149 (MQ=255)                                                                                                                  tGAAATATATGTAACCTGACTATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCGATGCCat                                    >  4:626430/1‑149 (MQ=255)                                                                                                                  tGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCat                                    >  4:639779/1‑149 (MQ=255)                                                                                                                  tGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCat                                    >  1:50617/1‑149 (MQ=255)                                                                                                                    aaaTATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCattt                                  >  4:365412/1‑149 (MQ=255)                                                                                                                       tatatGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCattttat                               <  3:560732/149‑1 (MQ=255)                                                                                                                        atatGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCattttatt                              <  3:217110/149‑1 (MQ=255)                                                                                                                         tatGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCattttattt                             <  3:644220/149‑1 (MQ=255)                                                                                                                           tGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCATTTTAttttt                           <  3:575078/149‑1 (MQ=255)                                                                                                                              aaCCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCAc                                                                          <  1:621998/99‑1 (MQ=255)                                                                                                                              aaCCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCAc                                                                          >  2:621998/1‑99 (MQ=255)                                                                                                                               aCCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTAAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCATTTTATTTTTGAAc                       >  3:315871/1‑149 (MQ=255)                                                                                                                                 cTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCattttattt                             >  3:668799/1‑141 (MQ=255)                                                                                                                                 cTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCattttattt                             <  4:668799/141‑1 (MQ=255)                                                                                                                                   gACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCATTTTATTTTGGAACGCAg                   <  3:637028/149‑1 (MQ=255)                                                                                                                                       aTCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAAttt                                                                                                                      <  2:519736/46‑1 (MQ=255)                                                                                                                                       aTCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAAttt                                                                                                                      >  1:519736/1‑46 (MQ=255)                                                                                                                                        tCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGAt                                                                                     >  1:373820/1‑78 (MQ=255)                                                                                                                                        tCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGAt                                                                                     <  2:373820/78‑1 (MQ=255)                                                                                                                                           cTTTTTATTCCTTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCATTTTATTTTTGAACGCAGGCCAATCa           >  1:513184/1‑149 (MQ=255)                                                                                                                                             ttttATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCATTTTATTTTTGAACGCAGGCCAATCATg         >  4:366816/1‑149 (MQ=255)                                                                                                                                                    ccGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCATTTTATTTTTGAACGCAGGCCAATCATGACTAACa  >  4:345136/1‑149 (MQ=255)                                                                                                                                                    ccGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCATTTTATTTTTGAACGCAGGCCAATCATGACTAACa  <  3:365412/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGGTCGATTTGAACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCGCCCGCTCGCGGCATATCCCGCTATGCCATTTTATTTTTGAACGCAGGCCAATCATGACTAACA  >  NC_000913/3492282‑3492577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑