Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,656,788 C→G 5.1% intergenic (‑48/‑207) hdeA ← / → hdeD stress response protein acid‑resistance protein/acid‑resistance membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,656,7880CG 5.1% 279.9 / 5.1 78intergenic (‑48/‑207)hdeA/hdeDstress response protein acid‑resistance protein/acid‑resistance membrane protein
Reads supporting (aligned to +/- strand):  ref base C (35/39);  new base G (2/2);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

ACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTAAATCGACTGCATTC  >  NC_000913/3656640‑3656929
                                                                                                                                                    |                                                                                                                                             
aCCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTc                                                                                                                                               >  3:160958/1‑149 (MQ=255)
  cGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGa                                                                                                                                             <  1:497044/149‑1 (MQ=255)
         ttGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGTAGAAGAAGCAGACCACCAAGAATAACGTCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAg                                                                                                                                      <  3:584923/149‑1 (MQ=255)
         ttGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAg                                                                                                                                      <  1:196468/149‑1 (MQ=255)
         ttGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAg                                                                                                                                      <  4:648628/149‑1 (MQ=255)
         ttGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAg                                                                                                                                      >  3:309711/1‑149 (MQ=255)
            ttATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGtt                                                                                                                                   >  4:95783/1‑149 (MQ=255)
               tCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTtata                                                                                                                                >  1:645348/1‑149 (MQ=255)
                cAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATaa                                                                                                                               <  3:529592/149‑1 (MQ=255)
                    tgctTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTGGACACTGAGGTTATAACCTg                                                                                                                           >  1:306528/1‑149 (MQ=255)
                    tgctTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTg                                                                                                                           >  2:332987/1‑149 (MQ=255)
                    tgctTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTg                                                                                                                           <  2:415081/149‑1 (MQ=255)
                       tttttGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGtt                                                                                                                        <  2:358304/149‑1 (MQ=255)
                           tGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTGGACACTGAGGTTATAACCTGGTTTTCt                                                                                                                    <  2:306528/149‑1 (MQ=255)
                           tGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCt                                                                                                                    <  4:549129/149‑1 (MQ=255)
                           tGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCt                                                                                                                    <  2:645348/149‑1 (MQ=255)
                             cgcATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGt                                                                                                                  <  1:169185/149‑1 (MQ=255)
                              gcATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGta                                                                                                                 >  2:446901/1‑149 (MQ=255)
                                aTCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGtata                                                                                                               >  1:660033/1‑149 (MQ=255)
                                 tCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTatat                                                                                                              >  1:687201/1‑149 (MQ=255)
                                    gCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTc                                                                                                           >  1:426776/1‑149 (MQ=255)
                                                  aaCTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGaaaa                                                                                             >  1:567269/1‑149 (MQ=255)
                                                  aaCTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGaaaa                                                                                             <  2:689924/149‑1 (MQ=255)
                                                         agaagaAGCAGTCCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCaaa                                                                                      >  3:393161/1‑149 (MQ=255)
                                                           aagaagCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTg                                                                                                                                        <  2:97544/97‑1 (MQ=255)
                                                           aagaagCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTg                                                                                                                                        >  1:97544/1‑97 (MQ=255)
                                                            agaagCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATc                                                                                   <  4:309711/149‑1 (MQ=255)
                                                             gaagCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCa                                                                                  >  4:239725/1‑149 (MQ=255)
                                                             gaagCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCa                                                                                  <  3:95783/149‑1 (MQ=255)
                                                             gaagCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCa                                                                                  <  2:687201/149‑1 (MQ=255)
                                                              aagCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGtt                                                                                                                                   >  4:502434/1‑99 (MQ=255)
                                                              aagCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGtt                                                                                                                                   <  3:502434/99‑1 (MQ=255)
                                                              aagCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAg                                                                                 <  2:408664/149‑1 (MQ=255)
                                                               agCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGa                                                                                <  4:170130/149‑1 (MQ=255)
                                                                gCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGat                                                                               >  1:646483/1‑149 (MQ=255)
                                                                  aGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGatat                                                                             <  3:239725/149‑1 (MQ=255)
                                                                   gACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCACCTCTAACGTGAAGGCGCCGTCACGAATCAATTTCGACCCTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATAtt                                                                            >  1:19792/1‑149 (MQ=255)
                                                                        ccaAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATACAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTCATGGAAAATATCAAAATCAGATATTTTTAt                                                                       >  1:448283/1‑149 (MQ=255)
                                                                        ccaAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTAt                                                                       <  1:332987/149‑1 (MQ=255)
                                                                                  cGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACaa                                                             <  2:660033/149‑1 (MQ=255)
                                                                                   gCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAAt                                                            <  2:567269/149‑1 (MQ=255)
                                                                                      tAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAg                                                         <  1:446901/149‑1 (MQ=255)
                                                                                         tACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATc                                                                                   <  2:438088/120‑1 (MQ=255)
                                                                                         tACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATc                                                                                   >  1:438088/1‑120 (MQ=255)
                                                                                          aCTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGa                                                                                                    <  3:239157/102‑1 (MQ=255)
                                                                                          aCTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGa                                                                                                    >  4:239157/1‑102 (MQ=255)
                                                                                          aCTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTAc                                                     >  3:224453/1‑149 (MQ=255)
                                                                                            ttttttCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGaaaa                                                                                             >  4:406851/1‑107 (MQ=255)
                                                                                            ttttttCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGaaaa                                                                                             <  3:406851/107‑1 (MQ=255)
                                                                                            ttttttCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCaa                                                                                       <  1:665510/113‑1 (MQ=255)
                                                                                            ttttttCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCaa                                                                                       >  2:665510/1‑113 (MQ=255)
                                                                                            ttttttCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAg                                                   >  4:591563/1‑149 (MQ=255)
                                                                                                    tCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCa                                           >  3:186377/1‑149 (MQ=255)
                                                                                                      gTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGa                                         <  2:646483/149‑1 (MQ=255)
                                                                                                          tatCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTa                                                                        <  1:508932/114‑1 (MQ=255)
                                                                                                          tatCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTa                                                                        >  2:508932/1‑114 (MQ=255)
                                                                                                          tatCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACt                                     <  2:426776/149‑1 (MQ=255)
                                                                                                             ccTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCa                                  >  1:5441/1‑149 (MQ=255)
                                                                                                               tCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTGGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGAt                                                                                                   >  3:242313/1‑82 (MQ=255)
                                                                                                               tCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTGGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGAt                                                                                                   <  4:242313/82‑1 (MQ=255)
                                                                                                               tCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAAt                                >  4:630644/1‑149 (MQ=255)
                                                                                                               tCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAAt                                >  1:204439/1‑149 (MQ=255)
                                                                                                                 aaCTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTa                              >  2:2081/1‑149 (MQ=255)
                                                                                                                   cTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAgg                            >  3:99062/1‑149 (MQ=255)
                                                                                                                         aGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAAttt                      <  2:5441/149‑1 (MQ=255)
                                                                                                                           tGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAAttttt                    <  3:591563/149‑1 (MQ=255)
                                                                                                                           tGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAAttttt                    <  2:448283/149‑1 (MQ=255)
                                                                                                                            gAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTtta                   <  2:19792/149‑1 (MQ=255)
                                                                                                                            gAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTtta                   <  3:572190/149‑1 (MQ=255)
                                                                                                                              aaGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTttatt                 >  4:397473/1‑149 (MQ=255)
                                                                                                                                 agCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTaaa              <  1:2081/149‑1 (MQ=255)
                                                                                                                                  gCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTAAAt             <  4:160958/149‑1 (MQ=255)
                                                                                                                                     gTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTAAATCGa          <  4:224453/149‑1 (MQ=255)
                                                                                                                                     gTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTAAATCGa          >  3:6386/1‑149 (MQ=255)
                                                                                                                                      tCACGAATCAATTTCGTAACTGAGGTTATAAACTGGTTTTCTGTATATGTCATGTTGATGCAAACTGTCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTAAATCGAc         <  4:196711/149‑1 (MQ=255)
                                                                                                                                      tCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTAAATCGAc         >  1:394538/1‑149 (MQ=255)
                                                                                                                                        aCGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATAAGTCATGTTGATGGAAAATATCAAAATCAAATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTAAATCGACta       >  3:196711/1‑148 (MQ=255)
                                                                                                                                             tCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTAAATCGACTGCATTc  >  4:582458/1‑149 (MQ=255)
                                                                                                                                             tCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTAAATCGACTGCATTc  >  1:313030/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
ACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAACGCCTAATACTTTTTTCATCGTAATATCCTCAACTATAAAGTGAAAGAGCCGTCACGAATCAATTTCGACACTGAGGTTATAACCTGGTTTTCTGTATATGTCATGTTGATGGAAAATATCAAAATCAGATATTTTTATTTCAATACAATGAGTTACAGATGCATCAGATACTGCAATTAGGAAATTTTTATTAAATCGACTGCATTC  >  NC_000913/3656640‑3656929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: