Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,702,835 G→A 5.1% L12L (CTG→TTG)  dppF ← dipeptide/heme ABC transporter ATPas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,702,8350GA 5.1% 325.1 / 4.6 97L12L (CTG→TTG) dppFdipeptide/heme ABC transporter ATPas
Reads supporting (aligned to +/- strand):  ref base G (40/52);  new base A (3/2);  total (43/54)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGG  >  NC_000913/3702689‑3702979
                                                                                                                                                  |                                                                                                                                                
aCCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCg                                                                                                                                                >  1:588392/1‑149 (MQ=255)
  cGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGgt                                                                                                                                              <  3:56675/149‑1 (MQ=255)
     aGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGACTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGgttgt                                                                                                                                           <  4:10305/149‑1 (MQ=255)
      gCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGgttgtt                                                                                                                                          <  4:498985/149‑1 (MQ=255)
       ccAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGttgttg                                                                                                                                         <  3:398688/149‑1 (MQ=255)
        cAGATTCGCCCACTACTGTCAGCGTTTTGCCACGTTCAAGTTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGc                                                                                                                                        >  1:200356/1‑149 (MQ=255)
         aGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCa                                                                                                                                       <  4:567755/149‑1 (MQ=255)
          gATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAg                                                                                                                                      <  2:165145/149‑1 (MQ=255)
          gATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAACCGCTGTTGCAg                                                                                                                                      <  3:55722/149‑1 (MQ=255)
           aTTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAACCGCTGTTGCAgg                                                                                                                                     <  4:533259/149‑1 (MQ=255)
             tCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGt                                                                                                                                   <  1:501980/149‑1 (MQ=255)
              cGCCCACTTCTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTg                                                                                                                                  <  2:563662/149‑1 (MQ=255)
                  cACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTCGTTGCAGGGTGGCct                                                                                                                              <  2:588392/149‑1 (MQ=255)
                   actactGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCctc                                                                                                                             <  4:495544/149‑1 (MQ=255)
                   actactGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCctc                                                                                                                             <  2:297442/149‑1 (MQ=255)
                     tactGCCAGCGTTTTTCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATACTGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCtt                                                                                                                           <  2:200356/149‑1 (MQ=255)
                     tactGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCtt                                                                                                                           <  3:635987/149‑1 (MQ=255)
                     tactGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCtt                                                                                                                           <  2:154683/149‑1 (MQ=255)
                       ctGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGc                                                                                                                         <  4:256750/149‑1 (MQ=255)
                          ccAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTa                                                                                                                      <  3:686000/149‑1 (MQ=255)
                          ccAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTa                                                                                                                      >  1:667224/1‑149 (MQ=255)
                           cAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGt                                                                                                                       <  3:321790/147‑1 (MQ=255)
                           cAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGt                                                                                                                       >  4:321790/1‑147 (MQ=255)
                           cAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTAc                                                                                                                     <  1:522610/149‑1 (MQ=255)
                             gCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTc                                                                                                                   <  1:574758/149‑1 (MQ=255)
                                ttttGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATa                                                                                                                <  2:684516/149‑1 (MQ=255)
                                      cACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCg                                                                                                          >  3:85632/1‑149 (MQ=255)
                                          ttCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCtt                                                                                                                           <  1:70962/128‑1 (MQ=255)
                                          ttCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCtt                                                                                                                           >  2:70962/1‑128 (MQ=255)
                                          ttCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCt                                                                                                      >  4:251451/1‑149 (MQ=255)
                                                gTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGgtt                                                                                                                                             >  3:104390/1‑104 (MQ=255)
                                                gTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGgtt                                                                                                                                             <  4:104390/104‑1 (MQ=255)
                                                     acgaaacgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATa                                                                                                                <  1:355500/128‑1 (MQ=255)
                                                     acgaaacgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATa                                                                                                                >  2:355500/1‑128 (MQ=255)
                                                       gaaacgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCAtcatca                                                                                         >  4:101723/1‑149 (MQ=255)
                                                          acgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAg                                                                                                               >  1:454788/1‑124 (MQ=255)
                                                          acgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAg                                                                                                               <  2:454788/124‑1 (MQ=255)
                                                          acgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGt                                                                                      <  4:85632/149‑1 (MQ=255)
                                                          acgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGt                                                                                      <  1:483672/149‑1 (MQ=255)
                                                          acgCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGt                                                                                      >  1:161224/1‑149 (MQ=255)
                                                            gCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTgg                                                                                    <  2:161224/149‑1 (MQ=255)
                                                               aTCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTa                                                                                 >  4:676089/1‑149 (MQ=255)
                                                                 ccAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAAt                                                                               <  4:459809/149‑1 (MQ=255)
                                                                 ccAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAAt                                                                               <  2:667224/149‑1 (MQ=255)
                                                                  cAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATg                                                                              <  3:259016/149‑1 (MQ=255)
                                                                   aGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATgg                                                                             >  2:612258/1‑149 (MQ=255)
                                                                        tttAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGt                                                                                      >  2:250315/1‑135 (MQ=255)
                                                                        tttAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGt                                                                                      <  1:250315/135‑1 (MQ=255)
                                                                         ttAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACGGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTg                                                                       <  4:297425/149‑1 (MQ=255)
                                                                         ttAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTg                                                                       <  1:616804/149‑1 (MQ=255)
                                                                         ttAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTg                                                                       <  3:101723/149‑1 (MQ=255)
                                                                           aaCCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCtt                                                                                                                           <  2:344998/95‑1 (MQ=255)
                                                                           aaCCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCtt                                                                                                                           >  1:344998/1‑95 (MQ=255)
                                                                             ccAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTaa                                                                                <  4:339737/136‑1 (MQ=255)
                                                                             ccAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTaa                                                                                >  3:339737/1‑136 (MQ=255)
                                                                             ccAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATg                                                                              <  3:621879/138‑1 (MQ=255)
                                                                             ccAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATg                                                                              >  4:621879/1‑138 (MQ=255)
                                                                              cAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTg                                                                  >  2:577571/1‑149 (MQ=255)
                                                                              cAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTg                                                                  <  4:249120/149‑1 (MQ=255)
                                                                                gACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGAc                                                                >  1:652885/1‑149 (MQ=255)
                                                                                 aCGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTACAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATAATCAAGTGGGTAATGGCATTTGGACTGACg                                                               >  1:449554/1‑149 (MQ=255)
                                                                                           gcgAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCg                                                     >  3:392643/1‑149 (MQ=255)
                                                                                             gAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTACAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAg                                                   <  2:449554/149‑1 (MQ=255)
                                                                                                 aTGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCata                                               >  2:574164/1‑149 (MQ=255)
                                                                                                 aTGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCata                                               >  3:483605/1‑149 (MQ=255)
                                                                                                 aTGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCata                                               <  3:119042/149‑1 (MQ=255)
                                                                                                  tGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCatat                                              >  2:101683/1‑149 (MQ=255)
                                                                                                  tGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCatat                                              >  2:252411/1‑149 (MQ=255)
                                                                                                   gCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTgg                                                                                    >  1:413033/1‑110 (MQ=255)
                                                                                                   gCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCAACATCAAGTgg                                                                                    <  2:413033/110‑1 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCa                                           >  4:406463/1‑149 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCa                                           >  2:407233/1‑149 (MQ=255)
                                                                                                     cTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCa                                           >  2:640401/1‑149 (MQ=255)
                                                                                                      tttCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATGGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAg                                          <  4:494696/149‑1 (MQ=255)
                                                                                                      tttCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCatat                                              >  1:139745/1‑145 (MQ=255)
                                                                                                      tttCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCatat                                              <  2:139745/145‑1 (MQ=255)
                                                                                                      tttCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCac                                          <  4:595778/149‑2 (MQ=255)
                                                                                                             aCCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGt                                   >  1:53112/1‑149 (MQ=255)
                                                                                                                 gATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGttctt                               >  2:104592/1‑149 (MQ=255)
                                                                                                                   tAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTc                                                                                                           >  1:507002/1‑71 (MQ=255)
                                                                                                                   tAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTc                                                                                                           <  2:507002/71‑1 (MQ=255)
                                                                                                                      tGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAgcg                          >  3:318971/1‑149 (MQ=255)
                                                                                                                       gTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAgcgc                         <  4:617894/149‑1 (MQ=255)
                                                                                                                        ttttttCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGcgcg                        >  1:514780/1‑149 (MQ=255)
                                                                                                                              cAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCt                  <  3:487124/149‑1 (MQ=255)
                                                                                                                               aGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTg                 >  4:243314/1‑149 (MQ=255)
                                                                                                                                ggTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGt                <  2:260043/149‑1 (MQ=255)
                                                                                                                                  tCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCa              <  1:577571/149‑1 (MQ=255)
                                                                                                                                     aTAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTg           <  4:392643/149‑1 (MQ=255)
                                                                                                                                         ccTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCg                                     <  3:544962/119‑1 (MQ=255)
                                                                                                                                         ccTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCg                                     >  4:544962/1‑119 (MQ=255)
                                                                                                                                         ccTGCAACAACCGCTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGCCGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCAt       >  1:126865/1‑149 (MQ=255)
                                                                                                                                         ccTGCAACAACCGCTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCAt       >  3:190917/1‑149 (MQ=255)
                                                                                                                                          cTGCAACAACCGCTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATa      >  1:411946/1‑149 (MQ=255)
                                                                                                                                              aaCAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAgggg  >  1:256682/1‑149 (MQ=255)
                                                                                                                                                 aGCGTTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATAtt                                             <  2:419632/103‑1 (MQ=255)
                                                                                                                                                 aGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATAtt                                             >  1:419632/1‑103 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAACATGCCTTTCTTCACCGGATAATGTTTTTTCAGGTCGATAGCCTGCAACAGCGGTTGTTGCAGGGTGGCCTCTTGCGTACTCATAGTGTCGGCCTCCCGGCATCATCAAGTGGGTAATGGCATTTGGACTGACGCCCGTCAGCGAGCATATTCAGCGCCGGTTCTTCAGCGCGACATCTGTCAGTGGCATAGGGG  >  NC_000913/3702689‑3702979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: