Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,890,952 G→T 7.5% V239V (GTG→GTT)  tnaB → tryptophan transporter of low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,890,9520GT 7.5% 188.8 / 5.4 57V239V (GTG→GTT) tnaBtryptophan transporter of low affinity
Reads supporting (aligned to +/- strand):  ref base G (21/31);  new base T (2/2);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

TTCCCGTATATCTTTATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTG  >  NC_000913/3890809‑3891099
                                                                                                                                               |                                                                                                                                                   
ttCCCGTATATCTTTATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTa                                                                                                                                                >  1:406403/1‑149 (MQ=255)
  cccGTATATCTTTATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTAtc                                                                                                                                              <  2:385818/149‑1 (MQ=255)
     gTATATCTTTATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTTATTTATctct                                                                                                                                           >  1:291261/1‑149 (MQ=255)
      tataTCTTTATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTCATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCtctt                                                                                                                                          <  2:620285/149‑1 (MQ=255)
       atatCTTTATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTTATTTATCtcttc                                                                                                                                         <  2:291261/149‑1 (MQ=255)
            tttATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGct                                                                                                                                    >  2:173727/1‑149 (MQ=255)
               aTGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGA‑TATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTAt                                                                                                                                <  4:19794/149‑1 (MQ=255)
                tGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTAt                                                                                                                                <  1:375219/149‑1 (MQ=255)
                 ggCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCtctt                                                                                                                                          <  1:295315/138‑1 (MQ=255)
                 ggCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCtctt                                                                                                                                          >  2:295315/1‑138 (MQ=255)
                     ttGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCAc                                                                                                                           <  3:611814/149‑1 (MQ=255)
                     ttGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCAc                                                                                                                           >  3:304975/1‑149 (MQ=255)
                       gCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCCCTATTGCACCa                                                                                                                         <  3:598103/149‑1 (MQ=255)
                              tgtCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGaa                                                                                                                  <  1:647359/149‑1 (MQ=255)
                                 cTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAatat                                                                                                               <  2:31937/149‑1 (MQ=255)
                                        cATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCga                                                                                                        <  1:50884/149‑1 (MQ=255)
                                            tGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGaaa                                                                                                    <  3:643552/149‑1 (MQ=255)
                                                   cACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTaa                                                                                             <  2:406403/149‑1 (MQ=255)
                                                   cACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTaa                                                                                             <  3:620545/149‑1 (MQ=255)
                                                    aCGGCAATTTTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAg                                                                                            <  1:118686/149‑1 (MQ=255)
                                                    aCGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAg                                                                                            <  1:341195/149‑1 (MQ=255)
                                                        cAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCttt                                                                                               >  1:487694/1‑142 (MQ=255)
                                                        cAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCttt                                                                                               <  2:487694/142‑1 (MQ=255)
                                                         aaTATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGAt                                                                                       >  1:242966/1‑149 (MQ=255)
                                                            atTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCtt                                                                                                <  3:374953/137‑1 (MQ=255)
                                                            atTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCtt                                                                                                >  4:374953/1‑137 (MQ=255)
                                                                ccAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAatat                                                                                                               >  3:80988/1‑118 (MQ=255)
                                                                ccAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAatat                                                                                                               <  4:80988/118‑1 (MQ=255)
                                                                 cAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATctcct                                                                               <  4:422036/149‑1 (MQ=255)
                                                                      tGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGaaa                                                                                                    >  1:129095/1‑123 (MQ=255)
                                                                      tGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGaaa                                                                                                    <  2:129095/123‑1 (MQ=255)
                                                                      tGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAggc                                                                          <  1:193128/149‑1 (MQ=255)
                                                                       gATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTAGTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAggcg                                                                         >  3:663394/1‑149 (MQ=255)
                                                                            tttGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAAc                                                                    >  2:46654/1‑149 (MQ=255)
                                                                                cTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTg                                                                >  1:401114/1‑149 (MQ=255)
                                                                                 tATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGaa                                                                                                                  <  4:313931/98‑1 (MQ=255)
                                                                                 tATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGaa                                                                                                                  >  3:313931/1‑98 (MQ=255)
                                                                                     gAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCg                                                           >  3:348679/1‑149 (MQ=255)
                                                                                                  tAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTTATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGt                                              >  3:350362/1‑149 (MQ=255)
                                                                                                    aGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTc                                            <  2:242966/149‑1 (MQ=255)
                                                                                                       tAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGCCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAGTCGTTCCTc                                         <  2:142471/149‑1 (MQ=255)
                                                                                                        aaTCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTg                                                        >  3:638255/1‑133 (MQ=255)
                                                                                                        aaTCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTg                                                        <  4:638255/133‑1 (MQ=255)
                                                                                                         aTCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCgg                                       <  4:304975/149‑1 (MQ=255)
                                                                                                         aTCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCgg                                       >  3:296581/1‑149 (MQ=255)
                                                                                                          tCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGc                                      >  2:540331/1‑149 (MQ=255)
                                                                                                             aaaGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTTATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCAcc                                   <  4:350362/149‑1 (MQ=255)
                                                                                                                   tGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAg                             >  4:682309/1‑149 (MQ=255)
                                                                                                                       aTTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACg                         >  2:167562/1‑149 (MQ=255)
                                                                                                                        tttGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACgg                        >  3:480645/1‑149 (MQ=255)
                                                                                                                        tttGGTTCGCTGCTGGCGCGGGTAAGTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACgg                        >  2:675018/1‑149 (MQ=255)
                                                                                                                          tGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCa                      <  4:348679/149‑1 (MQ=255)
                                                                                                                           ggTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCAt                     <  2:229530/149‑1 (MQ=255)
                                                                                                                                  tgctgGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAg              <  4:480645/149‑1 (MQ=255)
                                                                                                                                        cgcTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGc        <  1:46654/149‑1 (MQ=255)
                                                                                                                                           tGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCctg     >  1:143012/1‑149 (MQ=255)
                                                                                                                                              tGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCctgctg  <  2:143012/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TTCCCGTATATCTTTATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCAGCCTGATTATTTGCTATGGAAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATTTATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATCTCCTCAGGCGGCAACGTTGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTG  >  NC_000913/3890809‑3891099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑