Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,920,796 T→C 6.4% A31A (GCA→GCG atpF ← F0 sector of membrane‑bound ATP synthase, subunit b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,920,7960TC 6.4% 251.7 / 3.2 76A31A (GCA→GCGatpFF0 sector of membrane‑bound ATP synthase, subunit b
Reads supporting (aligned to +/- strand):  ref base T (38/33);  new base C (2/3);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAAC  >  NC_000913/3920648‑3920944
                                                                                                                                                    |                                                                                                                                                    
tGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCt                                                                                                                                                      <  3:475583/149‑1 (MQ=255)
tGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCt                                                                                                                                                      <  4:296932/149‑1 (MQ=255)
  ttCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGc                                                                                                                                                    <  3:291515/149‑1 (MQ=255)
      ccTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCAtt                                                                                                                                                >  2:147432/1‑149 (MQ=255)
           tCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATgg                                                                                                                                           >  3:11/1‑149 (MQ=255)
           tCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCCGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATgg                                                                                                                                           <  1:563868/149‑1 (MQ=255)
            cGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggc                                                                                                                                          <  2:686711/149‑1 (MQ=255)
            cGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggc                                                                                                                                          <  4:489184/149‑1 (MQ=255)
             gatgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcg                                                                                                                                         >  1:542580/1‑149 (MQ=255)
              atgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcgg                                                                                                                                        >  2:52481/1‑149 (MQ=255)
               tgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                                                                       <  1:624295/149‑1 (MQ=255)
               tgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATggcagc                                                                                                                                       <  1:299099/149‑1 (MQ=255)
                        gggCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTAc                                                                                                                              <  1:379596/149‑1 (MQ=255)
                            ttCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCa                                                                                                                          >  1:231713/1‑149 (MQ=255)
                            ttCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCa                                                                                                                          >  4:505712/1‑149 (MQ=255)
                            ttCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCa                                                                                                                          >  3:496266/1‑149 (MQ=255)
                              ccGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATg                                                                                                                        <  4:496266/149‑1 (MQ=255)
                                gctttcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGca                                                                                                                      >  4:282239/1‑149 (MQ=255)
                                  tttcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCAt                                                                                                                         <  4:255735/144‑1 (MQ=255)
                                  tttcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCAt                                                                                                                         >  3:255735/1‑144 (MQ=255)
                                      gctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacag                                                                                                                >  2:206132/1‑149 (MQ=255)
                                      gctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacag                                                                                                                >  2:602516/1‑149 (MQ=255)
                                        ttttttCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacagaa                                                                                                              >  4:318583/1‑149 (MQ=255)
                                              cAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACa                                                                                                        <  1:52481/149‑1 (MQ=255)
                                                gCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAgg                                                                                                      <  1:147432/149‑1 (MQ=255)
                                                   ggtcggtcGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt                                                                                                                            >  3:191017/1‑124 (MQ=255)
                                                   ggtcggtcGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt                                                                                                                            <  4:191017/124‑1 (MQ=255)
                                                    gtcggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAg                                                                                                       >  1:61522/1‑144 (MQ=255)
                                                    gtcggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAg                                                                                                       <  2:61522/144‑1 (MQ=255)
                                                     tcggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACaaa                                                                                                 >  3:88053/1‑149 (MQ=255)
                                                           gcgcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAt                                                                                           <  1:206132/149‑1 (MQ=255)
                                                             gcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtgg                                                                                         >  1:294704/1‑149 (MQ=255)
                                                               tGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGa                                                                                                     >  4:564523/1‑135 (MQ=255)
                                                               tGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGa                                                                                                     <  3:564523/135‑1 (MQ=255)
                                                                ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGGCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcct                                                                                      <  3:577581/149‑1 (MQ=255)
                                                                 gCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcctg                                                                                     <  4:11/149‑1 (MQ=255)
                                                                    tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGTCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcctggcc                                                                                  >  1:400112/1‑149 (MQ=255)
                                                                       gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAg                                                                               <  3:514920/149‑1 (MQ=255)
                                                                         aaggtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGa                                                                             >  3:548454/1‑149 (MQ=255)
                                                                            gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttg                                                                          >  1:477289/1‑149 (MQ=255)
                                                                            gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttg                                                                          >  2:347776/1‑149 (MQ=255)
                                                                             tcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgt                                                                         >  2:35077/1‑149 (MQ=255)
                                                                               aaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttg                                                                       >  2:629187/1‑149 (MQ=255)
                                                                               aaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAttgttg                                                                       >  3:84340/1‑149 (MQ=255)
                                                                                 ggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCg                                                                     <  3:282239/149‑1 (MQ=255)
                                                                                  gtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGt                                                                    >  2:433170/1‑149 (MQ=255)
                                                                                   tcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGtt                                                                   >  2:488616/1‑149 (MQ=255)
                                                                                   tcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGtt                                                                   >  4:615980/1‑149 (MQ=255)
                                                                                      ttATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcc                                                                                       >  3:628856/1‑126 (MQ=255)
                                                                                      ttATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcc                                                                                       <  4:628856/126‑1 (MQ=255)
                                                                                      ttATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAg                                                                >  1:249863/1‑149 (MQ=255)
                                                                                      ttATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAg                                                                <  3:505712/149‑1 (MQ=255)
                                                                                           tgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGc                                                                      >  1:637176/1‑138 (MQ=255)
                                                                                           tgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGc                                                                      <  2:637176/138‑1 (MQ=255)
                                                                                           tgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTca                                                           >  3:266858/1‑149 (MQ=255)
                                                                                              tCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAg                                                        >  1:515723/1‑149 (MQ=255)
                                                                                              tCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAg                                                        >  3:60627/1‑149 (MQ=255)
                                                                                                   cTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACaa                                                   <  1:433170/149‑1 (MQ=255)
                                                                                                       ggAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGcc                                               >  2:138224/1‑149 (MQ=255)
                                                                                                        gAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCct                                              <  2:294704/149‑1 (MQ=255)
                                                                                                            cAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAATGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAt                                          >  3:445127/1‑149 (MQ=255)
                                                                                                             aaGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAtt                                         <  4:88053/149‑1 (MQ=255)
                                                                                                               ggCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCTCAATGCCTCTATTTa                                       <  4:445127/149‑1 (MQ=255)
                                                                                                               ggCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTa                                       <  3:615980/149‑1 (MQ=255)
                                                                                                                gacGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACATCACAATGCCTCTATTTAg                                      <  2:249863/147‑1 (MQ=255)
                                                                                                                 ccGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGt                                     <  4:548454/149‑1 (MQ=255)
                                                                                                                      aGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACg                                >  4:321581/1‑149 (MQ=255)
                                                                                                                      aGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACg                                >  2:296797/1‑149 (MQ=255)
                                                                                                                         aaTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTc                             <  4:266858/149‑1 (MQ=255)
                                                                                                                          aTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCt                            <  1:35077/149‑1 (MQ=255)
                                                                                                                              cTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGata                        <  2:542580/149‑1 (MQ=255)
                                                                                                                               tttttGACGTTTTTCGATGGCTGCTATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTATCGTTCTGatat                       <  2:496008/149‑1 (MQ=255)
                                                                                                                               tttttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGatat                       >  4:453156/1‑149 (MQ=255)
                                                                                                                                   tGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGct                   <  2:515723/149‑1 (MQ=255)
                                                                                                                                           ttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTtaaat           <  3:453156/149‑1 (MQ=255)
                                                                                                                                                 ggCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGatat                       <  3:357581/131‑1 (MQ=255)
                                                                                                                                                 ggCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGatat                       >  4:357581/1‑131 (MQ=255)
                                                                                                                                                 ggCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGc     >  2:517710/1‑149 (MQ=255)
                                                                                                                                                    tGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAAc  <  1:488616/149‑1 (MQ=255)
                                                                                                                                                    tGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAAc  <  4:84340/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAAC  >  NC_000913/3920648‑3920944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: