Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,093,463	A→G	6.2%	S270S (AGT→AGC)	rhaD ←	rhamnulose‑1‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,093,463	0	A	G	6.2%	206.6 / 3.7	64	S270S (AGT→AGC)	rhaD	rhamnulose‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (28/32);  new base G (2/2);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

GTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCATCCA  >  NC_000913/4093316‑4093611                                                                                                                                                    |                                                                                                                                                     gTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCAc                                                                                                                                                     >  3:134660/1‑149 (MQ=255) gTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCAc                                                                                                                                                     >  3:270496/1‑149 (MQ=255)       aTGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGa                                                                                                                                               <  1:352018/149‑1 (MQ=255)       aTGCGCACCGTGAAATTTCAGCACTGCTTCCAGTTCTGGCCATATCGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGa                                                                                                                                               <  4:110852/149‑1 (MQ=255)        tGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGTGCGCTGGCgct                                                                                                                                              <  2:456816/149‑3 (MQ=255)          cgcACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTg                                                                                                                                            <  4:103863/149‑1 (MQ=255)           gcACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTgg                                                                                                                                           <  2:394898/149‑1 (MQ=255)            cACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGc                                                                                                                                          <  4:308005/149‑1 (MQ=255)                  gagaTTTCAGCACTGCTTCGAGTTCTGGCCAGCTGGGACTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACg                                                                                                                                    >  2:645388/1‑149 (MQ=255)                         cAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAAc                                                                                                                                     <  2:308185/141‑1 (MQ=255)                         cAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAAc                                                                                                                                     >  1:308185/1‑141 (MQ=255)                          aGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACg                                                                                                                            >  3:310075/1‑149 (MQ=255)                          aGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACg                                                                                                                            >  1:273282/1‑149 (MQ=255)                             aCTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCtt                                                                                                                         <  4:134660/149‑1 (MQ=255)                                  ttCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGa                                                                                                                    >  2:167546/1‑149 (MQ=255)                                    ccAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAgc                                                                                                                  <  2:605950/149‑1 (MQ=255)                                        ttCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGc                                                                                                                                   >  3:322214/1‑128 (MQ=255)                                        ttCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGc                                                                                                                                   <  4:322214/128‑1 (MQ=255)                                            ggCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCaa                                                                                                          <  1:645388/149‑1 (MQ=255)                                                   tGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATc                                                                                                            >  1:37126/1‑140 (MQ=255)                                                   tGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATc                                                                                                            <  2:37126/140‑1 (MQ=255)                                                   tGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCa                                                                                                   <  2:146401/149‑1 (MQ=255)                                                      gATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACgg                                                                                                >  3:51507/1‑149 (MQ=255)                                                        ttATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCt                                                                                              >  4:226056/1‑149 (MQ=255)                                                           tGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCt                                                                                                               <  3:271524/129‑1 (MQ=255)                                                           tGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCt                                                                                                               >  4:271524/1‑129 (MQ=255)                                                            gCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATg                                                                                          <  1:167546/149‑1 (MQ=255)                                                                 cGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTg                                                                                     <  1:276864/149‑1 (MQ=255)                                                                      aTACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCa                                                                                <  1:294445/149‑1 (MQ=255)                                                                          tctTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATgcc                                                                            >  1:173952/1‑149 (MQ=255)                                                                          tctTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATgcc                                                                            >  1:431394/1‑149 (MQ=255)                                                                                 gggCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCa                                                                                                   >  3:308107/1‑119 (MQ=255)                                                                                 gggCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCa                                                                                                   <  4:308107/119‑1 (MQ=255)                                                                                  ggCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCg                                                                    <  4:51507/149‑1 (MQ=255)                                                                                  ggCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCg                                                                    >  3:581811/1‑149 (MQ=255)                                                                                    cGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGaa                                                                  <  4:310075/149‑1 (MQ=255)                                                                                    cGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGaa                                                                  <  1:630396/149‑1 (MQ=255)                                                                                        ggggTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATaaa                                                              <  1:173003/149‑1 (MQ=255)                                                                                              tACCTGCATGACAAAGGCTTTGCGGATCATGTTCGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCa                                                        <  4:455976/149‑1 (MQ=255)                                                                                                ccTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACt                                                      >  1:22327/1‑149 (MQ=255)                                                                                                 cTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTa                                                     >  4:54150/1‑149 (MQ=255)                                                                                                  tGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTaa                                                    >  1:373197/1‑149 (MQ=255)                                                                                                   gCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAAt                                                   >  2:670753/1‑149 (MQ=255)                                                                                                          aaaGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTtgtg                                            >  4:167221/1‑149 (MQ=255)                                                                                                          aaaGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTtgtg                                            >  2:313598/1‑149 (MQ=255)                                                                                                            aGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCt                                          <  1:250582/149‑1 (MQ=255)                                                                                                                   gCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGAttttt                                   <  2:173952/149‑1 (MQ=255)                                                                                                                          aTGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGgt                            >  1:190409/1‑149 (MQ=255)                                                                                                                           tGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGATGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGgtg                           >  3:502573/1‑149 (MQ=255)                                                                                                                            gTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGgtgt                          <  3:54150/149‑1 (MQ=255)                                                                                                                             tttGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTc                         <  2:431394/149‑1 (MQ=255)                                                                                                                                gCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGAt                      <  4:581811/149‑1 (MQ=255)                                                                                                                                    cTTACAGCGCCAGCGCACTGGCGCGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCt                                 <  4:667705/134‑1 (MQ=255)                                                                                                                                    cTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCt                                 >  3:667705/1‑134 (MQ=255)                                                                                                                                      tACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAAcc                >  1:168316/1‑149 (MQ=255)                                                                                                                                       cactcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTc                                  <  4:190966/126‑1 (MQ=255)                                                                                                                                       cactcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTc                                  >  3:190966/5‑130 (MQ=255)                                                                                                                                       aCAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCg               >  2:466542/1‑149 (MQ=255)                                                                                                                                        actcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGa              >  3:262482/4‑149 (MQ=255)                                                                                                                                         agcgccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGaa             <  2:373197/149‑1 (MQ=255)                                                                                                                                         agcgccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGaa             <  2:168316/149‑1 (MQ=255)                                                                                                                                          gcgccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAg            <  2:22327/149‑1 (MQ=255)                                                                                                                                           cgccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACATGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAgg           >  4:514857/1‑149 (MQ=255)                                                                                                                                             ccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGtt         <  3:167221/149‑1 (MQ=255)                                                                                                                                                    aCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCATCCa  <  1:670753/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCATCCA  >  NC_000913/4093316‑4093611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑