Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,094,696 A→C 7.7% intergenic (‑424/+27) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,094,6960AC 7.7% 167.5 / 4.3 50intergenic (‑424/+27)rhaD/rhaArhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base A (27/19);  new base C (2/2);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAA  >  NC_000913/4094549‑4094839
                                                                                                                                                   |                                                                                                                                               
cGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAg                                                                                                                                                <  1:166691/149‑1 (MQ=34)
 gACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAgg                                                                                                                                               >  2:467193/1‑149 (MQ=35)
             tCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATGCGGCGCGAGcgcctt                                                                                                                                   >  4:543644/1‑147 (MQ=255)
                  tCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCg                                                                                                                              <  3:543644/149‑1 (MQ=11)
                         tACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATTCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAgtgt                                                                                                                       >  3:416112/1‑149 (MQ=255)
                                    ccGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCgcggc                                                                                                            >  1:558143/1‑149 (MQ=255)
                                     cGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCgcggcg                                                                                                           >  1:71973/1‑149 (MQ=255)
                                         cTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTc                                                                                                       >  3:294369/1‑149 (MQ=255)
                                                 cGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttc                                                                                               <  4:294369/149‑1 (MQ=255)
                                                 cGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttc                                                                                               >  4:642424/1‑149 (MQ=255)
                                                   gATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttctt                                                                                             >  1:56517/1‑149 (MQ=255)
                                                        ggCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTCCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCa                                                                                        >  3:84102/1‑149 (MQ=255)
                                                        ggCACTTGTGCCGCATCCGGCAATCAATGCCTGATGAGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCa                                                                                        >  4:653222/1‑149 (MQ=255)
                                                           aCTTGTGCTGCATCTGGCAATAAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCTGTCCCGTAGGCCGGACAAGGCACTCGCGCCGCATGCGGGAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATaa                                                                                     <  3:427296/149‑1 (MQ=18)
                                                           aCTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATaa                                                                                     <  2:71973/149‑1 (MQ=255)
                                                               gtgCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGccc                                                                                 >  3:551576/1‑149 (MQ=255)
                                                                  ccGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCa                                                                              >  2:248114/1‑149 (MQ=255)
                                                                        ccGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGctct                                                                        >  1:262382/1‑149 (MQ=255)
                                                                         cGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCtctc                                                                       <  4:551576/149‑1 (MQ=255)
                                                                              atcaatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGcc                                                                  >  4:38023/1‑149 (MQ=255)
                                                                                  atGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTc                                                              <  1:248114/149‑1 (MQ=255)
                                                                                    gCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGc                                                            <  1:652798/149‑1 (MQ=255)
                                                                                       tGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTAc                                                         <  3:642424/149‑1 (MQ=255)
                                                                                        gATGCGACGCTGTCGCGCCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGCCTCAAAATTTCTTTCTCAGAAGCCCGTACGCACTCCAGCCATTCGCTAcc                                                        <  4:416112/149‑1 (MQ=25)
                                                                                             gACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACctgctg                                                   >  3:125500/1‑149 (MQ=255)
                                                                                             gACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACctgctg                                                   >  3:323617/1‑149 (MQ=255)
                                                                                               cGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGc                                                 <  2:56517/149‑1 (MQ=255)
                                                                                                    tCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATc                                            <  2:262382/149‑1 (MQ=255)
                                                                                                    tCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATc                                            <  4:323617/149‑1 (MQ=255)
                                                                                                      gcgTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCg                                                                                                                              <  3:347787/65‑1 (MQ=14)
                                                                                                      gcgTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCg                                                                                                                              >  4:347787/1‑65 (MQ=14)
                                                                                                       cgTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTg                                         >  4:391454/1‑149 (MQ=255)
                                                                                                        gTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCa                                                                              >  3:14640/1‑111 (MQ=255)
                                                                                                        gTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCa                                                                              <  4:14640/111‑1 (MQ=255)
                                                                                                          cTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACg                                      >  2:408733/1‑149 (MQ=255)
                                                                                                            tATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGtt                                    <  4:125500/149‑1 (MQ=255)
                                                                                                             aTCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGc                                                                           <  3:416249/109‑1 (MQ=255)
                                                                                                             aTCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGc                                                                           >  4:416249/1‑109 (MQ=255)
                                                                                                             aTCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGa                                        <  2:542468/144‑1 (MQ=255)
                                                                                                             aTCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGa                                        >  1:542468/1‑144 (MQ=255)
                                                                                                              tCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTgg                                  <  3:653222/149‑1 (MQ=255)
                                                                                                              tCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTgg                                  <  1:467193/149‑1 (MQ=255)
                                                                                                                    cTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATAc                            >  2:648335/1‑149 (MQ=255)
                                                                                                                     tACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACa                           >  1:8294/1‑149 (MQ=255)
                                                                                                                        aaCTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACAttt                        >  3:102806/1‑149 (MQ=255)
                                                                                                                                  gACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGc              >  4:563937/1‑149 (MQ=255)
                                                                                                                                    cccGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCACACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCt            <  2:558143/149‑1 (MQ=255)
                                                                                                                                     ccGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTg           >  2:639590/1‑149 (MQ=255)
                                                                                                                                             cGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCa   >  4:620612/1‑149 (MQ=255)
                                                                                                                                              ggATAAGCCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGCCaa  >  4:284961/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAA  >  NC_000913/4094549‑4094839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑