Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,223,854 T→C 5.8% R9R (CGT→CGC)  metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,8540TC 5.8% 229.4 / 3.3 69R9R (CGT→CGC) metHhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand):  ref base T (34/31);  new base C (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCA  >  NC_000913/4223706‑4224002
                                                                                                                                                    |                                                                                                                                                    
cGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTATATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGGGCGGCATAGTGGATCAACtacgt                                                                                                                                                      <  2:435385/149‑1 (MQ=255)
 gATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgtg                                                                                                                                                     >  1:462019/1‑149 (MQ=255)
      gTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAg                                                                                                                                                >  3:14178/1‑149 (MQ=255)
       ttAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGt                                                                                                                                               >  2:120409/1‑149 (MQ=255)
         aaTGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTa                                                                                                                                             >  4:103006/1‑149 (MQ=255)
           tGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaaa                                                                                                                                           <  4:564757/149‑1 (MQ=255)
           tGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgtt                                                                                                                                           <  1:120409/149‑4 (MQ=255)
            gTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAAt                                                                                                                                          <  4:407760/149‑1 (MQ=255)
             ttGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGAAGAAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATg                                                                                                                                         >  1:525246/1‑149 (MQ=255)
               gAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGaa                                                                                                                                       <  2:235382/149‑1 (MQ=255)
                      tctcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTc                                                                                                                                >  3:675397/1‑149 (MQ=255)
                       ctcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCt                                                                                                                               >  2:509364/1‑149 (MQ=255)
                        tcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg                                                                                                                              >  3:237109/1‑149 (MQ=255)
                        tcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg                                                                                                                              <  3:160025/149‑1 (MQ=255)
                        tcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg                                                                                                                              <  2:186691/149‑1 (MQ=255)
                           tGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTg                                                                                                                           <  4:675397/149‑1 (MQ=255)
                                cGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGa                                                                                                                      <  3:103006/149‑1 (MQ=255)
                                       gtCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGt                                                                                                               >  3:255790/1‑149 (MQ=255)
                                         cGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTAt                                                                                                             <  4:237109/149‑1 (MQ=255)
                                          gCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATg                                                                                                            >  3:561380/1‑149 (MQ=255)
                                            ttttACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATggg                                                                                                          >  4:321624/1‑149 (MQ=255)
                                                  cacaGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCAt                                                                                                    >  4:127061/1‑149 (MQ=255)
                                                  cacaGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCAt                                                                                                    >  4:88506/1‑149 (MQ=255)
                                                  cacaGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCAt                                                                                                    >  2:649760/1‑149 (MQ=255)
                                                     aGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATAGGCACCATGAt                                                                                                 <  4:14178/149‑1 (MQ=255)
                                                           gTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCagag                                                                                           >  2:151361/1‑149 (MQ=255)
                                                             ttATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGtt                                                                                         <  3:492396/149‑1 (MQ=255)
                                                                 gCCAGTATGGTTGGTTGAATTTTTATTAAATCTTGGGTGAGCGTGGCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAAAGTATTCTGGTGCTGGACGGCGGTATGGGCACAATGATCCAGAGTTATCg                                                                                     >  4:583896/1‑149 (MQ=255)
                                                                  ccAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGa                                                                                    <  1:367488/149‑1 (MQ=255)
                                                                        tGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAAc                                                                              <  1:434472/149‑1 (MQ=255)
                                                                          gTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGa                                                                            >  1:685861/1‑149 (MQ=255)
                                                                          gTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGa                                                                            <  3:127061/149‑1 (MQ=255)
                                                                               ttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGa                                                                                                  <  1:191308/122‑1 (MQ=255)
                                                                               ttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGa                                                                                                  >  2:191308/1‑122 (MQ=255)
                                                                               ttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCg                                                                       >  4:437642/1‑149 (MQ=255)
                                                                                tgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGa                                                                      <  2:636262/149‑1 (MQ=255)
                                                                                     ttttATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTc                                                                 <  2:462019/149‑1 (MQ=255)
                                                                                       ttattaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgt                                                               <  4:255790/149‑1 (MQ=255)
                                                                                          ttaAATCTGGGTTGAGCGTGTCGGGATCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTAt                                                                                       <  1:490619/122‑1 (MQ=255)
                                                                                          ttaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTAt                                                                                       >  2:490619/1‑122 (MQ=255)
                                                                                          ttaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtggt                                                            >  2:327908/1‑149 (MQ=255)
                                                                                             aaTCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTgcgc                                                                                                                                                  <  3:448582/60‑1 (MQ=255)
                                                                                             aaTCTGGGTTAAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTgcgc                                                                                                                                                  >  4:448582/1‑60 (MQ=255)
                                                                                              aTCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAtt                                                                    <  1:590917/137‑1 (MQ=255)
                                                                                              aTCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAtt                                                                    >  2:590917/1‑137 (MQ=255)
                                                                                              aTCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAAc                                                        <  3:126145/149‑1 (MQ=255)
                                                                                              aTCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAAc                                                        >  3:52131/1‑149 (MQ=255)
                                                                                                        aGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTc                                                                 <  3:356097/130‑1 (MQ=255)
                                                                                                        aGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTc                                                                 >  4:356097/1‑130 (MQ=255)
                                                                                                           gtgtCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTg                                           <  1:151361/149‑1 (MQ=255)
                                                                                                           gtgtCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTg                                           <  1:649760/149‑1 (MQ=255)
                                                                                                                 ggAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATg                                     >  3:600230/1‑149 (MQ=255)
                                                                                                                    gCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGa                                  >  1:641569/1‑149 (MQ=255)
                                                                                                                     cAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGAc                                 >  4:218005/1‑149 (MQ=255)
                                                                                                                       aGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCt                               <  2:641569/149‑1 (MQ=255)
                                                                                                                        gtgtGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTc                              >  3:676173/1‑149 (MQ=255)
                                                                                                                           tgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCaaa                           <  4:561380/149‑1 (MQ=255)
                                                                                                                            gAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAg                          <  3:437642/149‑1 (MQ=255)
                                                                                                                            gAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAg                          <  4:600230/149‑1 (MQ=255)
                                                                                                                            gAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAg                          >  1:430740/1‑149 (MQ=255)
                                                                                                                             agcagcAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAgg                         >  4:371430/1‑149 (MQ=255)
                                                                                                                              gcagcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGc                        <  1:509364/149‑1 (MQ=255)
                                                                                                                                       tGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCaga                                                                                            <  3:150509/72‑1 (MQ=255)
                                                                                                                                       tGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCaga                                                                                            >  4:150509/1‑72 (MQ=255)
                                                                                                                                         gAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACct             >  4:345630/1‑149 (MQ=255)
                                                                                                                                         gAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACct             <  3:321624/149‑1 (MQ=255)
                                                                                                                                         atttaacTGCGCACGCAGTTAAATGAACGTATTCTGGAGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAAGGAAGCCGATTTTCGTGGTGAACGCTTTGCGGACTGGCCATGCGACGTCAAAGGCAACAACAACct             >  2:320691/5‑149 (MQ=255)
                                                                                                                                          aacaacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctg            >  2:648556/1‑149 (MQ=255)
                                                                                                                                          aacaacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctg            >  1:285004/1‑149 (MQ=255)
                                                                                                                                           acaacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctgc           >  4:274745/1‑149 (MQ=255)
                                                                                                                                             aacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCGGGACGGCGGTTTGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctgctg         <  1:320691/149‑1 (MQ=255)
                                                                                                                                                tgcgtgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTa      >  4:615585/1‑149 (MQ=255)
                                                                                                                                                    tgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCa  <  2:285004/149‑1 (MQ=255)
                                                                                                                                                    tgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCa  >  4:66973/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCA  >  NC_000913/4223706‑4224002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑