Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,289,094 T→A 5.4% I444N (ATT→AAT)  nrfA → nitrite reductase, formate‑dependent, cytochrome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,289,0940TA 5.4% 332.1 / 7.3 93I444N (ATT→AAT) nrfAnitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base T (39/49);  new base A (2/3);  total (41/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01

GGAAGAAGGTTTACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGA  >  NC_000913/4288948‑4289242
                                                                                                                                                  |                                                                                                                                                    
ggAAGAAGGTTTACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTg                                                                                                                                                    <  3:330777/149‑1 (MQ=255)
ggAAGAAGGTTTACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTg                                                                                                                                                    <  4:314217/149‑1 (MQ=255)
 gaagaaGGTTTACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTgg                                                                                                                                                   >  3:589864/1‑149 (MQ=255)
  aagaagGTTTACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGc                                                                                                                                                  <  1:605515/149‑1 (MQ=255)
   agaagGTTTACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGcc                                                                                                                                                 >  3:384029/1‑149 (MQ=255)
   agaagGTTTACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGcc                                                                                                                                                 <  2:628712/149‑1 (MQ=255)
      agGTTTACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGa                                                                                                                                              <  4:375725/149‑1 (MQ=255)
         tttACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCAATGGCCTgctgg                                                                                                                                           <  4:239772/149‑5 (MQ=255)
          ttACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACAt                                                                                                                                          <  1:293333/149‑1 (MQ=255)
           tACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATg                                                                                                                                         <  3:83866/149‑1 (MQ=255)
              ggATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGaa                                                                                                                                      <  3:55408/149‑1 (MQ=255)
                  gCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACaaa                                                                                                                                  <  1:119333/149‑1 (MQ=255)
                  gCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACaaa                                                                                                                                  >  3:251736/1‑149 (MQ=255)
                  gCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACaaa                                                                                                                                  <  3:108028/149‑1 (MQ=255)
                       gTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAg                                                                                                                             <  3:250622/149‑1 (MQ=255)
                            gCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCga                                                                                                                        <  3:103535/149‑1 (MQ=255)
                             cGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCgag                                                                                                                       <  4:285859/149‑1 (MQ=255)
                              gATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCgaga                                                                                                                      >  2:165415/1‑149 (MQ=255)
                                  gATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCa                                                                                                                  <  2:31586/149‑1 (MQ=255)
                                   aTAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCCAAGGCATCACCCAGGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAATCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAg                                                                                                                 <  4:463822/149‑1 (MQ=255)
                                   aTAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAg                                                                                                                 <  2:490223/149‑1 (MQ=255)
                                   aTAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAg                                                                                                                 <  2:476589/149‑1 (MQ=255)
                                   aTAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAg                                                                                                                 <  1:615291/149‑1 (MQ=255)
                                     aaaGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGa                                                                                                               <  4:325659/149‑1 (MQ=255)
                                      aaGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCAGGCGACCTAAGGCATCACCCAAGAAAGCCAGATCCCGGATATCTCAACCACAGAGAAAGCCCAGCAGGCCATTGGCCTGCACATGGAACAAATCAAGGCCGAGCAGCAGGAc                                                                                                              >  3:431066/1‑149 (MQ=255)
                                            cggATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTtcatc                                                                                                        <  2:431616/149‑1 (MQ=255)
                                            cggATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTtcatc                                                                                                        <  4:221847/149‑1 (MQ=255)
                                                  cacgcacCAAACTGGCGCGCCTGGTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACg                                                                                                  <  4:480462/149‑1 (MQ=255)
                                                   acgcacCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACgg                                                                                                 <  4:384029/149‑1 (MQ=255)
                                                       acCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGAt                                                                                             >  1:160456/1‑149 (MQ=255)
                                                        ccAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATc                                                                                            <  4:251736/149‑1 (MQ=255)
                                                          aaaCTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATccc                                                                                          >  1:65194/1‑149 (MQ=255)
                                                           aaCTGGCGCGCCTGCTGGCGACCAAAGGCATTACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATg                                                                                                                                         >  1:453528/1‑101 (MQ=255)
                                                           aaCTGGCGCGCCTGCTGGCGACCAAAGGCATTACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATg                                                                                                                                         <  2:453528/101‑1 (MQ=255)
                                                              tGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAg                                                                                      >  2:591016/1‑149 (MQ=255)
                                                                  gcgcCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTggg                                                                                  <  3:541730/149‑1 (MQ=255)
                                                                      ctgctgGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGgaaga                                                                              >  2:443974/1‑149 (MQ=255)
                                                                       tgctgGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGgaagaa                                                                             >  4:2949/1‑149 (MQ=255)
                                                                       tgctgGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGgaagaa                                                                             <  1:443974/149‑1 (MQ=255)
                                                                            gCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGc                                                                        >  3:486725/1‑149 (MQ=255)
                                                                                 cAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTa                                                                   >  2:167227/1‑149 (MQ=255)
                                                                                 cAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTa                                                                   >  2:133103/1‑149 (MQ=255)
                                                                                   aaGGCATCACGCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGCCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTAAACAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTaaa                                                                 >  4:448920/1‑149 (MQ=255)
                                                                                     ggCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTaaaaa                                                               <  1:165415/149‑1 (MQ=255)
                                                                                      gCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAg                                                                          <  3:423489/137‑1 (MQ=255)
                                                                                      gCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAg                                                                          >  4:423489/1‑137 (MQ=255)
                                                                                      gCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAg                                                                          >  4:316433/1‑137 (MQ=255)
                                                                                      gCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAg                                                                          <  3:316433/137‑1 (MQ=255)
                                                                                         tCACGCATGAAATCCAGATCCCGGATATCTCAACCAAAGTGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGCAGCAGGAGTTCATCAAAACGGTGATCCCGCAGTGGGTAGAACAGGCACGTAAAAACGGt                                                           <  4:431066/149‑1 (MQ=255)
                                                                                         tCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGt                                                           <  2:577034/149‑1 (MQ=255)
                                                                                           aCCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCt                                                         <  1:591016/149‑1 (MQ=255)
                                                                                             ccATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGt                                                                                                >  2:668109/1‑108 (MQ=255)
                                                                                             ccATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGt                                                                                                <  1:668109/108‑1 (MQ=255)
                                                                                             ccATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGt                                                       >  3:431646/1‑149 (MQ=255)
                                                                                                tGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGaa                                                                                                                     >  3:381988/1‑84 (MQ=255)
                                                                                                tGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGaa                                                                                                                     <  4:381988/84‑1 (MQ=255)
                                                                                                 gAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAg                                                   <  3:316196/149‑1 (MQ=255)
                                                                                                    aTCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCa                                                >  3:4552/1‑149 (MQ=255)
                                                                                                     tCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGTTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCaa                                               >  1:408622/1‑149 (MQ=255)
                                                                                                     tCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCaa                                               >  2:510028/1‑149 (MQ=255)
                                                                                                     tCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCaa                                               <  1:261436/149‑1 (MQ=255)
                                                                                                      ccAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAAt                                              >  4:643721/1‑149 (MQ=255)
                                                                                                        aGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCTAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGc                                                  <  2:494018/143‑1 (MQ=255)
                                                                                                        aGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGc                                                  >  1:494018/1‑143 (MQ=255)
                                                                                                        aGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATaa                                            <  4:589864/149‑1 (MQ=255)
                                                                                                         gATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAAc                                           >  2:174532/1‑149 (MQ=255)
                                                                                                           tCCCGGATATCTCAACCAAAGAGAAATCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCTAAACGGGGATCCCAGAGTAGGAAGAACAGACACGTAAAAACGGACTGTTAAGCCAATAAccc                                         >  2:178159/1‑149 (MQ=255)
                                                                                                                gATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACa                                                                           >  3:295035/1‑110 (MQ=255)
                                                                                                                gATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACa                                                                           <  4:295035/110‑1 (MQ=255)
                                                                                                                     ctcAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGccc                               >  2:17718/1‑148 (MQ=255)
                                                                                                                      tcAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAgg                                                                         <  4:482576/106‑1 (MQ=255)
                                                                                                                      tcAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAgg                                                                         >  3:482576/1‑106 (MQ=255)
                                                                                                                      tcAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTc                              >  2:156113/1‑149 (MQ=255)
                                                                                                                          ccAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCaa                          <  1:133103/149‑1 (MQ=255)
                                                                                                                           cAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGtt                                                      <  1:279129/120‑1 (MQ=255)
                                                                                                                           cAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGtt                                                      >  2:279129/1‑120 (MQ=255)
                                                                                                                           cAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAg                         >  2:416628/1‑149 (MQ=255)
                                                                                                                            aaaGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAgg                        >  1:128724/1‑149 (MQ=255)
                                                                                                                             aaGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAggg                       >  3:232130/1‑149 (MQ=255)
                                                                                                                               gagaAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGc                     >  4:254287/1‑149 (MQ=255)
                                                                                                                                    aGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGaaa                <  2:160456/149‑1 (MQ=255)
                                                                                                                                       ccAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAAcccc                                        >  1:289173/1‑122 (MQ=255)
                                                                                                                                       ccAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAAcccc                                        <  2:289173/122‑1 (MQ=255)
                                                                                                                                       ccAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAaca             <  1:174532/149‑1 (MQ=255)
                                                                                                                                       ccAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAaca             <  1:156113/149‑1 (MQ=255)
                                                                                                                                        gttcagGCCAATGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTaaaa                                                                >  2:253178/4‑97 (MQ=255)
                                                                                                                                        gttcagGCCAATGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTaaaa                                                                <  1:253178/94‑1 (MQ=255)
                                                                                                                                         ttcagGCCAATGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTc                              <  1:632063/128‑1 (MQ=255)
                                                                                                                                         ttcagGCCAATGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTc                              >  2:632063/3‑130 (MQ=255)
                                                                                                                                         agcagGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAAcaca           >  2:420822/1‑149 (MQ=255)
                                                                                                                                          gcagGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACaa          <  2:65194/149‑1 (MQ=255)
                                                                                                                                            agGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATg        <  2:408622/149‑1 (MQ=255)
                                                                                                                                                 aTTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTg   >  1:361207/1‑149 (MQ=255)
                                                                                                                                                  ttGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGa  >  3:357599/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGAAGAAGGTTTACGGATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTGGCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCCCAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAAACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCGGAAAACACAATGGAGTGA  >  NC_000913/4288948‑4289242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑