Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,337,523 A→G 7.9% V136A (GTT→GCT)  adiY ← adi system transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,337,5230AG 7.9% 239.0 / 7.2 75V136A (GTT→GCT) adiYadi system transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (38/31);  new base G (3/3);  total (41/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTTTCAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTA  >  NC_000913/4337375‑4337670
                                                                                                                                                    |                                                                                                                                                   
gCTTTTCAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCGGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGaaa                                                                                                                                                     >  4:434474/1‑149 (MQ=255)
  ttttCAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACg                                                                                                                                                   >  1:124780/1‑149 (MQ=255)
                tttAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTCACATATACATCATTAATGAAACGAATTTTTTACTGTc                                                                                                                                     >  3:482274/1‑149 (MQ=255)
                tttAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTc                                                                                                                                     >  2:191178/1‑149 (MQ=255)
                  tAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCga                                                                                                                                   <  4:208519/149‑1 (MQ=255)
                   aaCAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTc                                                                                                                                     >  4:315919/1‑146 (MQ=255)
                   aaCAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTc                                                                                                                                     <  3:315919/146‑1 (MQ=255)
                    aCAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAAttttt                                                                                                                                            >  2:675151/1‑138 (MQ=255)
                    aCAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAAttttt                                                                                                                                            <  1:675151/138‑1 (MQ=255)
                    aCAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCgaga                                                                                                                                 >  2:514245/1‑149 (MQ=255)
                          ttGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACgg                                                                                                                           <  1:191178/149‑1 (MQ=255)
                          ttGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACgg                                                                                                                           >  4:314956/1‑149 (MQ=255)
                          ttGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACgg                                                                                                                           >  2:584531/1‑149 (MQ=255)
                            gggCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGa                                                                                                                         <  1:536923/149‑1 (MQ=255)
                             ggCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGAt                                                                                                                        >  3:15220/1‑149 (MQ=255)
                                tAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGc                                                                                                                     >  1:20013/1‑149 (MQ=255)
                                tAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGc                                                                                                                     >  3:461221/1‑149 (MQ=255)
                                       tAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGa                                                                                                              >  2:569015/1‑149 (MQ=255)
                                           caacTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGaataa                                                                                                          <  4:15220/149‑1 (MQ=255)
                                            aacTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTTACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGaataat                                                                                                         <  4:523376/149‑1 (MQ=255)
                                                 gCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATgc                                                                                                    >  4:127835/1‑149 (MQ=255)
                                                           tAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTgttcgt                                                                                          >  3:17611/1‑149 (MQ=255)
                                                           tAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTgttcgt                                                                                          >  1:601124/1‑149 (MQ=255)
                                                           tAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTgttcgt                                                                                          >  1:472832/1‑149 (MQ=255)
                                                             aGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTgttcgttc                                                                                        <  3:626375/149‑1 (MQ=255)
                                                             aGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTgttcgttc                                                                                        <  4:383600/149‑1 (MQ=255)
                                                               aTTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCCTGTTCGTTCCt                                                                                      <  1:514245/149‑1 (MQ=255)
                                                                ttCCAGTCTTTGTGAATATTGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCtt                                                                                     <  2:124780/149‑1 (MQ=255)
                                                                ttCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCtt                                                                                     >  3:495594/1‑149 (MQ=255)
                                                                  ccAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCtttt                                                                                   <  4:17611/149‑1 (MQ=255)
                                                                   cAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTc                                                                                  <  1:584531/149‑1 (MQ=255)
                                                                      tCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGa                                                                               <  2:472832/149‑1 (MQ=255)
                                                                      tCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGa                                                                               <  3:314956/149‑1 (MQ=255)
                                                                        tttGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGaa                                                                                                             <  4:48649/117‑1 (MQ=255)
                                                                        tttGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGaa                                                                                                             >  3:48649/1‑117 (MQ=255)
                                                                              aaTATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGttt                                                                       >  3:357308/1‑149 (MQ=255)
                                                                                tatCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCt                                                                     <  3:434474/149‑1 (MQ=255)
                                                                                  tCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGc                                                                   >  3:268243/1‑149 (MQ=255)
                                                                                          aataatTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGcatca                                                           >  1:253400/1‑149 (MQ=255)
                                                                                          aataatTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGcat                                                             <  1:601995/147‑1 (MQ=255)
                                                                                          aataatTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGcat                                                             >  2:601995/1‑147 (MQ=255)
                                                                                            taatTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATg                                                         >  3:373211/1‑149 (MQ=255)
                                                                                               tttGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCtg                                                      >  1:116424/1‑149 (MQ=255)
                                                                                                   aTAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGtt                                                  >  1:632981/1‑149 (MQ=255)
                                                                                                   aTAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGtt                                                  >  2:69242/1‑149 (MQ=255)
                                                                                                    tAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAGCGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCtgt                                                     <  4:681492/145‑1 (MQ=255)
                                                                                                    tAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAGCGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCtgt                                                     >  3:681492/1‑145 (MQ=255)
                                                                                                    tAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAGCGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCtgt                                                     >  1:183559/1‑145 (MQ=255)
                                                                                                    tAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAGCGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCtgt                                                     <  2:183559/145‑1 (MQ=255)
                                                                                                    tAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAGCGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCtgt                                                     <  4:569063/145‑1 (MQ=255)
                                                                                                    tAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAGCGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCAGCGGGCATCATGCtgt                                                     >  3:569063/1‑145 (MQ=255)
                                                                                                    tAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTg                                                 >  2:105997/1‑149 (MQ=255)
                                                                                                       aCGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCtgt                                                     >  1:376757/1‑142 (MQ=255)
                                                                                                       aCGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCtgt                                                     <  2:376757/142‑1 (MQ=255)
                                                                                                         gCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGaataa                                                                                                          >  3:508538/1‑87 (MQ=255)
                                                                                                         gCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGaataa                                                                                                          <  4:508538/87‑1 (MQ=255)
                                                                                                           tGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCa                                          >  4:681457/1‑149 (MQ=255)
                                                                                                               aCTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAAcgc                                      <  2:614728/149‑1 (MQ=255)
                                                                                                                   acacAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGGATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGaaa                                  <  4:274443/149‑1 (MQ=255)
                                                                                                                   acacAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGaaa                                  <  3:127835/149‑1 (MQ=255)
                                                                                                                   acacAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGaaa                                  >  2:65473/1‑149 (MQ=255)
                                                                                                                     acaATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGa                                >  4:132771/1‑149 (MQ=255)
                                                                                                                       aaTTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACg                              >  3:12962/1‑149 (MQ=255)
                                                                                                                       aaTTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACg                              >  2:415704/1‑149 (MQ=255)
                                                                                                                            cGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCCTCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTgg                         <  1:415704/149‑1 (MQ=255)
                                                                                                                               aaCATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGt                      <  2:20013/149‑1 (MQ=255)
                                                                                                                                aCATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTc                     >  1:175336/1‑149 (MQ=255)
                                                                                                                                  atatACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAg                   <  2:175336/149‑1 (MQ=255)
                                                                                                                                       catcatTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGCGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATg              >  3:87229/1‑149 (MQ=255)
                                                                                                                                        atcatTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATgg             <  2:481516/149‑1 (MQ=255)
                                                                                                                                         tcatTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGc            >  4:505669/1‑149 (MQ=255)
                                                                                                                                           atTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTc                                                                                  >  4:231616/1‑77 (MQ=255)
                                                                                                                                           atTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTc                                                                                  <  3:231616/77‑1 (MQ=255)
                                                                                                                                              aaTGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGt       <  4:268243/149‑1 (MQ=255)
                                                                                                                                              aaTGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGt       <  3:132771/149‑1 (MQ=255)
                                                                                                                                              aaTGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGt       <  4:357308/149‑1 (MQ=255)
                                                                                                                                                   aaCGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTa  >  4:73628/1‑149 (MQ=255)
                                                                                                                                                   aaCGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTa  >  3:388871/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCTTTTCAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTA  >  NC_000913/4337375‑4337670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: