Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,514,336 A→T 6.3% intergenic (‑28/+17) fecB ← / ← fecA ferric citrate ABC transporter periplasmic binding protein/TonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,514,3360AT 6.3% 218.9 / 5.1 63intergenic (‑28/+17)fecB/fecAferric citrate ABC transporter periplasmic binding protein/TonB‑dependent outer membrane ferric citrate transporter and signal transducer; ferric citrate extracelluar receptor; FecR‑interacting protein
Reads supporting (aligned to +/- strand):  ref base A (28/31);  new base T (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

TCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCA  >  NC_000913/4514191‑4514477
                                                                                                                                                 |                                                                                                                                             
tCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGcc                                                                                                                                            >  1:631027/1‑149 (MQ=255)
   gTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCggg                                                                                                                                         <  4:584132/149‑1 (MQ=255)
         gCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTaa                                                                                                                                   <  2:327787/149‑1 (MQ=255)
         gCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTaa                                                                                                                                   <  3:601707/149‑1 (MQ=255)
         gCGTTTTTTCGAGTGCAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGACAAATGCCAACATAATCACATTCCAGCTGAAAGCCCGGCAAGCCGGGCGTTaa                                                                                                                                   <  2:200409/149‑1 (MQ=255)
           gTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAaca                                                                                                                                 <  3:667102/149‑1 (MQ=255)
                ttCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCa                                                                                                                            <  1:353087/149‑1 (MQ=255)
                ttCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCa                                                                                                                            <  3:511163/149‑1 (MQ=255)
                ttCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCa                                                                                                                            <  4:362485/149‑1 (MQ=255)
                    aGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGcc                                                                                                                                            >  1:54815/1‑129 (MQ=255)
                    aGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGcc                                                                                                                                            <  2:54815/129‑1 (MQ=255)
                    aGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAAc                                                                                                                        >  4:296608/1‑149 (MQ=255)
                        tAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCa                                                                                                                    >  4:510042/1‑149 (MQ=255)
                             gTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGAc                                                                                                               <  3:296608/149‑1 (MQ=255)
                                         tCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGAccc                                                                                                             <  1:143966/139‑1 (MQ=255)
                                         tCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGAccc                                                                                                             >  2:143966/1‑139 (MQ=255)
                                          ccTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTg                                                                                                          >  3:153274/1‑141 (MQ=255)
                                          ccTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTg                                                                                                          <  4:153274/141‑1 (MQ=255)
                                           cTGAACCGTGGCGGCACAGGCGTGGCTGATCACCAGCCGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAccg                                                                                                 >  2:551074/1‑147 (MQ=255)
                                            tGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGc                                                                                                >  4:242135/1‑149 (MQ=255)
                                             gAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCg                                                                                               >  3:506902/1‑149 (MQ=255)
                                             gAACCGTGGCGGCAAAGGCGTGGCTGATCACAAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCg                                                                                               >  3:566819/1‑149 (MQ=255)
                                              aaCCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGt                                                                                              <  3:242135/149‑1 (MQ=255)
                                                  gTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTgcgc                                                                                          <  4:506902/149‑1 (MQ=255)
                                                  gTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTgcgc                                                                                          >  4:10298/1‑149 (MQ=255)
                                                  gTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTgcgc                                                                                          <  1:433797/149‑1 (MQ=255)
                                                          aaaGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGt                                                                                              <  1:136097/137‑1 (MQ=255)
                                                          aaaGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGt                                                                                              >  2:136097/1‑137 (MQ=255)
                                                          aaaGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGcc                                                                                  >  3:169563/1‑149 (MQ=255)
                                                                gTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCTTGCCGGGCtttt                                                                                                                                    >  4:238481/1‑92 (MQ=255)
                                                                gTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCTTGCCGGGCtttt                                                                                                                                    <  3:238481/93‑2 (MQ=255)
                                                                  ggCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGa                                                                          <  4:269460/149‑1 (MQ=255)
                                                                     tGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGc                                                                       >  2:139929/1‑149 (MQ=255)
                                                                       aTCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCt                                                                     >  3:164046/1‑149 (MQ=255)
                                                                       aTCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCt                                                                     <  3:510042/149‑1 (MQ=255)
                                                                          aCCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTttg                                                                  >  3:661808/1‑149 (MQ=255)
                                                                           ccAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTttgt                                                                 <  2:631027/149‑1 (MQ=255)
                                                                                  cagGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTgtc                                                          <  1:621834/149‑1 (MQ=255)
                                                                                      ccTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCa                                                      <  1:551074/149‑1 (MQ=255)
                                                                                       cTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCAt                                                     >  3:300048/1‑149 (MQ=255)
                                                                                             aaaGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGc                                               >  2:399055/1‑149 (MQ=255)
                                                                                              aaGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCg                                              <  1:139929/149‑1 (MQ=255)
                                                                                                gAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGa                                            <  4:169563/149‑1 (MQ=255)
                                                                                                   aaCGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGa                                         >  1:105215/1‑149 (MQ=255)
                                                                                                      ggATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAagt                                      >  3:66148/1‑149 (MQ=255)
                                                                                                      ggATAAATGCCAACATAATCACATTCCACCTAAAAGCCCGGCAAGCCGGGCGTTACCACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGCCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAagt                                      >  4:482714/1‑149 (MQ=255)
                                                                                                       gATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTttgtt                                                                >  1:478297/1‑122 (MQ=255)
                                                                                                       gATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTttgtt                                                                <  2:478297/122‑1 (MQ=255)
                                                                                                       gATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAagta                                     <  4:566819/149‑1 (MQ=255)
                                                                                                          aaaTGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTc                                  >  3:587589/1‑149 (MQ=255)
                                                                                                                 aaCATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAagag                                                >  4:653130/1‑128 (MQ=255)
                                                                                                                 aaCATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAagag                                                <  3:653130/128‑1 (MQ=255)
                                                                                                                      ggtCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGAt                      <  4:275125/147‑1 (MQ=255)
                                                                                                                        tCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGt                    >  4:488213/1‑149 (MQ=255)
                                                                                                                        tCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGt                    >  3:343203/1‑149 (MQ=255)
                                                                                                                            aTTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGttttt                <  3:488213/149‑1 (MQ=255)
                                                                                                                             ttCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGaa                                        <  4:211049/124‑1 (MQ=255)
                                                                                                                             ttCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGaa                                        >  3:211049/1‑124 (MQ=255)
                                                                                                                              tCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATa                                                                            <  3:57236/87‑1 (MQ=255)
                                                                                                                              tCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATa                                                                            >  4:57236/1‑87 (MQ=255)
                                                                                                                                    ttaacgCCCGGCTTGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGa        >  4:594339/6‑149 (MQ=255)
                                                                                                                                         gCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGcc   <  1:362595/149‑1 (MQ=255)
                                                                                                                                          cccGGCTTGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCa  <  3:594339/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCA  >  NC_000913/4514191‑4514477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑