Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 440,663 T→G 5.3% S147R (AGC→CGC) ‡ ispA ← geranyltranstransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913440,6630TG 5.3% 298.7 / 5.9 84S147R (AGC→CGC) ‡ispAgeranyltranstransferase
Reads supporting (aligned to +/- strand):  ref base T (35/44);  new base G (3/2);  total (38/46)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAGGTTGGC  >  NC_000913/440521‑440808
                                                                                                                                              |                                                                                                                                                 
gCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAg                                                                                                                                             <  3:231454/149‑1 (MQ=255)
  aCCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGtt                                                                                                                                           >  1:297625/1‑149 (MQ=255)
  aCCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGAGCGCCAGtg                                                                                                                                           <  3:659892/149‑2 (MQ=255)
  aCCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGAGCGCCAGtg                                                                                                                                           >  4:659892/1‑148 (MQ=255)
   ccAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGAGCGCCAGtgg                                                                                                                                          <  1:664398/149‑3 (MQ=255)
    cAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCa                                                                                                                                         >  3:585939/1‑149 (MQ=255)
    cAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCa                                                                                                                                         <  4:23034/149‑1 (MQ=255)
    cAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCa                                                                                                                                         >  2:558914/1‑149 (MQ=255)
    cAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCa                                                                                                                                         >  1:455689/1‑149 (MQ=255)
    cAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCa                                                                                                                                         <  2:610462/149‑1 (MQ=255)
      aGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGa                                                                                                                                       >  2:463375/1‑149 (MQ=255)
      aGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGa                                                                                                                                       <  3:345556/149‑1 (MQ=255)
           aaCGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAatca                                                                                                                                  <  2:223891/149‑1 (MQ=255)
            aCGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAatcat                                                                                                                                 <  1:12590/149‑1 (MQ=255)
            aCGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAatcat                                                                                                                                 <  3:515550/149‑1 (MQ=255)
             cggcggCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAatcatc                                                                                                                                <  2:297625/149‑1 (MQ=255)
               gcggcgCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGa                                                                                                                              <  1:559299/149‑1 (MQ=255)
               gcggcgCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGa                                                                                                                              >  2:213989/1‑149 (MQ=255)
                 ggcgCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAatcatc                                                                                                                                <  1:181220/145‑1 (MQ=255)
                 ggcgCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAatcatc                                                                                                                                >  2:181220/1‑145 (MQ=255)
                 ggcgCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGaaa                                                                                                                            <  1:617073/149‑1 (MQ=255)
                 ggcgCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGaaa                                                                                                                            <  4:131295/149‑1 (MQ=255)
                 ggcgCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGaaa                                                                                                                            <  2:549113/149‑1 (MQ=255)
                       aaTCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGt                                                                                                                      >  1:514453/1‑149 (MQ=255)
                       aaTCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGt                                                                                                                      >  3:42969/1‑149 (MQ=255)
                                 ccGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGa                                                                                                            <  2:167964/149‑1 (MQ=255)
                                  cGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGac                                                                                                           >  3:682438/1‑149 (MQ=255)
                                  cGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGac                                                                                                           <  2:232/149‑1 (MQ=255)
                                    gTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTc                                                                                                                     >  2:310648/1‑137 (MQ=255)
                                    gTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTc                                                                                                                     <  1:310648/137‑1 (MQ=255)
                                    gTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGacac                                                                                                         <  2:685917/149‑1 (MQ=255)
                                    gTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGacac                                                                                                         >  4:402158/1‑149 (MQ=255)
                                        tATGACGATGAATACGCTCAATCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTATTGTCTGACCACCGCACATTTCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTACGACACTTcc                                                                                                     <  3:425960/149‑1 (MQ=255)
                                         aTGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAGATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCg                                                                                                    <  4:318064/149‑1 (MQ=255)
                                          tGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTc                                                                                                                                          >  4:304774/1‑110 (MQ=255)
                                          tGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTc                                                                                                                                          <  3:304774/110‑1 (MQ=255)
                                          tGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCgg                                                                                                   >  3:651851/1‑149 (MQ=255)
                                                tGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATc                                                                                             <  3:298849/149‑1 (MQ=255)
                                                 gAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCg                                                                                            <  2:57603/149‑1 (MQ=255)
                                                     aCGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCAt                                                                                        <  2:588985/149‑1 (MQ=255)
                                                         tCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCt                                                                                    >  3:8955/1‑149 (MQ=255)
                                                             gcgcGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTaaa                                                                                >  4:263938/1‑149 (MQ=255)
                                                                 gTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCg                                                                            <  3:2404/149‑1 (MQ=255)
                                                                 gTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCg                                                                            >  4:287156/1‑149 (MQ=255)
                                                                  tCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCga                                                                           <  2:410478/149‑1 (MQ=255)
                                                                  tCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCga                                                                           <  1:463375/149‑1 (MQ=255)
                                                                  tCCAGAGGTACATGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCCGCATCGCTTAAAATCga                                                                           <  4:578424/149‑1 (MQ=255)
                                                                             gtgtTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCg                                                                >  3:353662/1‑149 (MQ=255)
                                                                                tttGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGttt                                                             <  3:439405/149‑1 (MQ=255)
                                                                                tttGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGttt                                                             <  4:353662/149‑1 (MQ=255)
                                                                                 ttGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAAAACGCTAGCGTTTg                                                            >  3:578495/1‑149 (MQ=255)
                                                                                     cTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTaaa                                                        >  4:582845/1‑149 (MQ=255)
                                                                                     cTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTaaa                                                        >  4:210821/1‑149 (MQ=255)
                                                                                      ttCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAg                                                       >  1:558783/1‑149 (MQ=255)
                                                                                                   tCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAatcatc                                                                                                                                >  2:162493/1‑63 (MQ=255)
                                                                                                   tCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAatcatc                                                                                                                                <  1:162493/63‑1 (MQ=255)
                                                                                                     tAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGaa                                        >  4:265477/1‑149 (MQ=255)
                                                                                                            tGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGtt                                 <  2:455689/149‑1 (MQ=255)
                                                                                                              accaccGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTg                               >  4:216968/1‑149 (MQ=255)
                                                                                                                caccGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCt                             >  4:19474/1‑149 (MQ=255)
                                                                                                                 accGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCtt                            <  1:213989/149‑1 (MQ=255)
                                                                                                                    gCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGt                                                                                                                      <  1:312645/56‑1 (MQ=255)
                                                                                                                    gCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGt                                                                                                                      >  2:312645/1‑56 (MQ=255)
                                                                                                                        aTTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCaaa                     >  1:125942/1‑149 (MQ=255)
                                                                                                                        aTTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCaaa                     >  1:293856/1‑149 (MQ=255)
                                                                                                                           ccGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACtt                  >  2:71682/1‑149 (MQ=255)
                                                                                                                           ccGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACtt                  >  2:440528/1‑149 (MQ=255)
                                                                                                                             ggCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTca                >  1:516664/1‑149 (MQ=255)
                                                                                                                              gCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTcac               <  4:682438/149‑1 (MQ=255)
                                                                                                                              gCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTcac               <  2:558783/149‑1 (MQ=255)
                                                                                                                              gCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTcac               >  2:541425/1‑149 (MQ=255)
                                                                                                                              gCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTcac               <  3:582845/149‑1 (MQ=255)
                                                                                                                                aaTACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACAt             <  4:8955/149‑1 (MQ=255)
                                                                                                                                 tctgaaCTGGCGAGCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGCGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATg            >  2:241128/6‑149 (MQ=255)
                                                                                                                                 aTACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATg            <  2:125942/149‑1 (MQ=255)
                                                                                                                                 aTACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATg            <  2:293856/149‑1 (MQ=255)
                                                                                                                                   tgaaCTGGCGAGCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGc          >  4:636995/4‑149 (MQ=255)
                                                                                                                                    ccACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATc                                      <  1:675038/120‑1 (MQ=255)
                                                                                                                                    ccACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATc                                      >  2:675038/1‑120 (MQ=255)
                                                                                                                                      aCTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAgg       <  3:402158/149‑1 (MQ=255)
                                                                                                                                       cTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAGGt      >  3:100029/1‑149 (MQ=255)
                                                                                                                                          gcgcTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAGGTTgg   <  1:71682/149‑1 (MQ=255)
                                                                                                                                          gcgcTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAGGTTgg   <  1:440528/149‑1 (MQ=255)
                                                                                                                                           cgcTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAGGTTGGc  <  4:585939/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCTGACCACCGCACATTCCGGCAATACCACTGGCGCTCGCCAGTTCAGAAATCATCGAAATTCTGTCGCGGTCCGACACTTCCGGCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATCGCGTTTGCTTCGCCAAACTTCACATGGCAGGTTGGC  >  NC_000913/440521‑440808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: