Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,608,607 C→G 7.9% I141M (ATC→ATG)  rimI → ribosomal‑protein‑S18‑alanine N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,608,6070CG 7.9% 253.8 / 10.2 75I141M (ATC→ATG) rimIribosomal‑protein‑S18‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (25/43);  new base G (3/3);  total (28/47)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

GGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCT  >  NC_000913/4608460‑4608753
                                                                                                                                                   |                                                                                                                                                  
ggAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatca                                                                                                                                                   <  2:329292/149‑1 (MQ=255)
 gAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCcatcatcat                                                                                                                                                  >  1:355606/1‑149 (MQ=255)
  aaaaaCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATg                                                                                                                                                 <  4:27502/149‑1 (MQ=255)
   aaaaCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATgg                                                                                                                                                <  2:59102/149‑1 (MQ=255)
    aaaCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGc                                                                                                                                               <  4:148900/149‑1 (MQ=255)
      aCGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATGATGATGGCGt                                                                                                                                             <  1:337390/149‑1 (MQ=255)
       cgcgGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGtt                                                                                                                                            <  1:158260/149‑1 (MQ=255)
         cggcgTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGc                                                                                                                                          <  1:280610/149‑1 (MQ=255)
           gcgTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATGATGATGGCGtcttcg                                                                                                                                        <  3:473603/149‑6 (MQ=255)
            cgTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCaa                                                                                                                                       <  2:512758/149‑1 (MQ=255)
            cgTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCaa                                                                                                                                       <  4:611680/149‑1 (MQ=255)
            cgTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCaa                                                                                                                                       <  1:362487/149‑1 (MQ=255)
                 cGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGt                                                                                                                                  <  4:599073/149‑1 (MQ=255)
                  gACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTa                                                                                                                                 <  4:145249/149‑1 (MQ=255)
                  gACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTa                                                                                                                                 >  1:276461/1‑149 (MQ=255)
                         tGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATa                                                                                                                          <  2:513226/149‑1 (MQ=255)
                             tGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAg                                                                                                                      >  3:204871/1‑149 (MQ=255)
                             tGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAg                                                                                                                      <  3:3453/149‑1 (MQ=255)
                                aaGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATTATGGCGTTGCCAATCAGTATGTAATACAAGGTg                                                                                                                   <  4:568181/149‑1 (MQ=255)
                                   tCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaa                                                                                                                >  3:219083/1‑149 (MQ=255)
                                   tCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaa                                                                                                                >  1:237523/1‑149 (MQ=255)
                                     cGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGaata                                                                                                              <  2:378737/149‑1 (MQ=255)
                                      gTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATGATGATGGCGtc                                                                                                                                            >  1:594845/1‑117 (MQ=255)
                                      gTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATGATGATGGCGtc                                                                                                                                            <  2:594845/118‑2 (MQ=255)
                                      gTGCTTCAAACGCTGCCGCCATTGCCCTGTACCAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACACGGTGGaataa                                                                                                             >  4:440618/1‑149 (MQ=255)
                                          ttCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGaa                                                                                                         <  2:355606/149‑1 (MQ=255)
                                             aaaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTg                                                                                                      <  2:276461/149‑1 (MQ=255)
                                              aaCGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTgg                                                                                                     <  3:345599/149‑1 (MQ=255)
                                                 gCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGAc                                                                                                  <  3:236040/149‑1 (MQ=255)
                                                 gCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGAc                                                                                                  >  2:74754/1‑149 (MQ=255)
                                                     ccgccATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGa                                                                                              <  4:219083/149‑1 (MQ=255)
                                                     ccgccATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGa                                                                                              <  4:331247/149‑1 (MQ=255)
                                                         cATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGAtttt                                                                                          <  3:399012/149‑1 (MQ=255)
                                                            tGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCtt                                                                                       <  3:440548/149‑1 (MQ=255)
                                                               ccTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGa                                                                                    <  3:386478/149‑1 (MQ=255)
                                                                cTGTACGAAAGTTTAGGCTTTATCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGAt                                                                                   >  1:171391/1‑149 (MQ=255)
                                                                     cGAAAGTTTAGGTTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                      <  3:419278/141‑1 (MQ=255)
                                                                     cGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCttt                                                                                      >  4:419278/1‑141 (MQ=255)
                                                                      gAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATa                                                                                                                          <  2:41445/104‑1 (MQ=255)
                                                                      gAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATa                                                                                                                          >  1:41445/1‑104 (MQ=255)
                                                                                  tttAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGttt                                                                 >  2:26184/1‑149 (MQ=255)
                                                                                   ttAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGt                                                                   >  4:344536/1‑146 (MQ=255)
                                                                                   ttAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGt                                                                   <  3:344536/146‑1 (MQ=255)
                                                                                     aaCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTAcc                                                              <  4:204871/149‑1 (MQ=255)
                                                                                      aCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGAt                                                                                   >  3:480591/1‑127 (MQ=255)
                                                                                      aCGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGAt                                                                                   <  4:480591/127‑1 (MQ=255)
                                                                                       cGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCtt                                                            <  1:209775/149‑1 (MQ=255)
                                                                                        gAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCttt                                                           <  4:63014/149‑1 (MQ=255)
                                                                                        gAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCttt                                                           <  2:171391/149‑1 (MQ=255)
                                                                                          ggCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGa                                                         <  1:74754/149‑1 (MQ=255)
                                                                                           gCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTACGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGa                                                                           >  1:674394/1‑130 (MQ=255)
                                                                                           gCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTACGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGa                                                                           <  2:674394/130‑1 (MQ=255)
                                                                                             gacgaTTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTc                                                      >  3:447208/1‑149 (MQ=255)
                                                                                              acgaTTCGCCACAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTgg                                                                                               <  1:457532/107‑1 (MQ=255)
                                                                                              acgaTTCGCCACAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTgg                                                                                               >  2:457532/1‑107 (MQ=255)
                                                                                                  ttCGCCGCAATTACTACCCCAACACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCa                                                 >  2:624334/1‑149 (MQ=255)
                                                                                                         cAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCt                                          <  1:395850/149‑1 (MQ=255)
                                                                                                            ttACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGc                                                                      <  1:221627/118‑1 (MQ=255)
                                                                                                            ttACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGc                                                                      >  2:221627/1‑118 (MQ=255)
                                                                                                                 acCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAAGCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGa                                  <  4:211503/149‑1 (MQ=255)
                                                                                                                  ccccACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGAt                                 >  3:412209/1‑149 (MQ=255)
                                                                                                                   cccACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCtt                                                                                       <  3:132254/94‑1 (MQ=255)
                                                                                                                   cccACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCtt                                                                                       >  4:132254/1‑94 (MQ=255)
                                                                                                                     caccacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGtt                              >  3:186281/1‑149 (MQ=255)
                                                                                                                      accacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCttt                                                           <  4:21021/119‑1 (MQ=255)
                                                                                                                      accacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCttt                                                           >  3:21021/1‑119 (MQ=255)
                                                                                                                      accacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCttt                                                           <  2:661843/119‑1 (MQ=255)
                                                                                                                      accacGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCttt                                                           >  1:661843/1‑119 (MQ=255)
                                                                                                                           ggacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGa                        <  1:624334/149‑1 (MQ=255)
                                                                                                                            gacggTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATCTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGAt                       <  3:440618/149‑1 (MQ=255)
                                                                                                                                      aaGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCa             <  1:26184/149‑1 (MQ=255)
                                                                                                                                        aaCGCCATGATGATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGCCTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAcc           >  3:461754/2‑149 (MQ=255)
                                                                                                                                        aaCGCCATGATGATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCAcc           >  1:3753/2‑149 (MQ=255)
                                                                                                                                           gCCATCATCATGGCGTTGCCAATCAGTATGTAATACGAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCttt        >  4:653194/1‑149 (MQ=255)
                                                                                                                                           gCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGa                        >  2:419961/1‑133 (MQ=255)
                                                                                                                                           gCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGa                        <  1:419961/133‑1 (MQ=255)
                                                                                                                                           gCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCttt        >  4:324439/1‑149 (MQ=255)
                                                                                                                                             catcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTAc      >  2:2217/1‑149 (MQ=255)
                                                                                                                                              atcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCt                           <  4:636467/127‑1 (MQ=255)
                                                                                                                                              atcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCt                           >  3:636467/1‑127 (MQ=255)
                                                                                                                                               tcatcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCg    <  1:266005/149‑1 (MQ=255)
                                                                                                                                                 atcatGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCt  <  3:324439/149‑1 (MQ=255)
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GGAAAAACGCGGCGTGGCGACACTATGGCTGGAAGTCCGTGCTTCAAACGCTGCCGCCATTGCCCTGTACGAAAGTTTAGGCTTTAACGAGGCGACGATTCGCCGCAATTACTACCCCACCACGGACGGTCGCGAAGACGCCATCATCATGGCGTTGCCAATCAGTATGTAATACAAGGTGGAATAATGAAGTGGGACTGGATTTTCTTTGATGCCGATGAAACGCTGTTTACCTTTGACTCATTCACCGGCCTGCAGCGGATGTTTCTTGATTACAGCGTCACCTTTACCGCT  >  NC_000913/4608460‑4608753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑