Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,875,5210TC20.6% 129.3 / 13.2 69A28A (GCT→GCCyidXputative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (26/28);  new base C (14/0);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 2.01e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGTTTGCCGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTA  >  NC_000913/3875251‑3875796
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                    
gcgTTTGCAGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAAAACACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAAGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGAGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGtt                                                                                                                                                                                                                                                                                <  2:382912/277‑1 (MQ=255)
      gCCGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTACGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATa                                                                                                                                                                                                                                                                         >  2:125270/1‑278 (MQ=255)
         gtgcgtgTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCAt                                                                                                                                                                                                                                                                          <  3:198796/274‑1 (MQ=255)
                   tttagtaCAAACTTATATTGTAGTACTAAAATTTAGAACACAAAAAGAACAATGCATAAAAAAATGACATGCGTCAGGTAGAAATCTGAAAAGGGATATCAGTCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAATGATTAATCAGGAAGTTGCATTTTAAGGGTTTTTTTAAGGCAGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCGCGTATGCTCTGGTTTCCCATACCGCAAAggg                                                                                                                                                                                                                                                               <  3:273779/273‑1 (MQ=255)
                        taaaaagtaatatTGTTTGACTACAATTTAGATCACAAAAAGAACAAAGCAAAAAAACATAACTTGGGTAGGACATAAATCTGAAAAGGGATATCAGGCGCTAAACCAGGTTGAATGAAGAGTATCCTTTCAACGGTTTAACTACGCGGTTAAAGGATTCAACATTAAGTTGAAATTTAAGAAATTTGTTAAGGCCGCCGGTTAAGTCATACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAATGGTAGtt                                                                                                                                                                                                                                                          <  3:589522/266‑1 (MQ=255)
                        taCAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGTTTTCCTATACCGCAAAGGGTAGtt                                                                                                                                                                                                                                                          >  1:410817/1‑275 (MQ=255)
                        taCAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGAGTTTTTAAGGCTGTCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCATGTTTCCCATACCGCAAAGGGTAGtt                                                                                                                                                                                                                                                          >  1:255971/1‑275 (MQ=255)
                             aCTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTCGTCTGCTTGAAAGAAAAGGTTAGTACAGGAAAg                                                                                                                                                                                                                                                  >  2:266810/1‑278 (MQ=255)
                             aCTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCCCGTATGCTCTGGTTTCCCATACCGCAAAGGGAAGTTCAGGAAAg                                                                                                                                                                                                                                                  >  2:99431/1‑278 (MQ=255)
                                  tatTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTa                                                                                                                                                                                                                                                >  1:458142/1‑275 (MQ=255)
                                  tatTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTAAGGAACGTAtaa                                                                                                                                                                                                                                             >  4:427446/1‑276 (MQ=255)
                                       ttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGGTTTCCCATACCGCAAAGGGTAGTTCCGGAAAGAATCAAt                                                                                                                                                                                                                                           >  1:519669/1‑275 (MQ=255)
                                       ttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCCTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGTAAAGGGTAGTTCAGGAAAGTATCAAt                                                                                                                                                                                                                                           >  1:53697/1‑275 (MQ=255)
                                       ttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACGGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGCAAGTATCAATCGc                                                                                                                                                                                                                                        >  2:132622/1‑278 (MQ=255)
                                       ttgtACTAAAATTTAGATAACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGATAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAt                                                                                                                                                                                                                                           <  3:244229/275‑1 (MQ=255)
                                         gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTTTCTCGTAGGCTCTGGTTTCCCCTACAGACAAGGCTAGTTAAGGAAATTATCAATCg                                                                                                                                                                                                                                         >  3:59617/1‑275 (MQ=255)
                                         gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGGTTTTCCTGACAAAAAAGGGTAGTTAAGGAAATGAACAATCg                                                                                                                                                                                                                                         >  1:320996/1‑275 (MQ=255)
                                         gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATc                                                                                                                                                                                                                                          >  1:203792/1‑274 (MQ=255)
                                                         cacaAAAAGAAAAAAGCATAAAAAAATAAGAGGCGACGGGCAGAAAACTGAAAAAGGATATCAAGAGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGAGTAATAATGAAGTTGAATTTGAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCAc                                                                                                                                                                                                                        <  4:314827/276‑1 (MQ=255)
                                                                      aTGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAACAAAAAAGGTAGTGCAGGAAAGTATAAATAGTAGTCAGACACAATCCATGGGCTATCGAAgta                                                                                                                                                                                                        >  2:541648/1‑276 (MQ=255)
                                                                            aaaaaaaTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCTTGTTTTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACCCCATCACTGGGCTATCGCAGGCAAAAGa                                                                                                                                                                                                  >  2:10345/1‑278 (MQ=255)
                                                                            aaaaaaaTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTTACAAAAAAGAGTAGTTCAGGAAAGTATCAATCGCAATCAGAAACCATCACTGTGCTATCGCAGGCaaa                                                                                                                                                                                                     >  1:405138/1‑275 (MQ=255)
                                                                                   tGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAgg                                                                                                                                                                                                                                                                >  2:561809/1‑210 (MQ=255)
                                                                                   tGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGTTCTGCTGTACAGCAAAAGGTAGTTCAGGAAAGTATCAATCGCAGCAAGACACCATCACTGGGCTATCGCAGGCAAAAGATAg                                                                                                                                                                                               >  1:419264/1‑274 (MQ=255)
                                                                                   tGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGTTCTCCCATTCCGCAAAGGGTAGTTCAGGAAAGTATCAATCCCAGTCAGAACCCATGAATGGGCTATCCCAGGCAAAagagag                                                                                                                                                                                               >  1:500657/1‑274 (MQ=255)
                                                                                    gACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAggg                                                                                                                                                                                                                                                               <  4:561809/210‑1 (MQ=255)
                                                                                     aCATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGACTATCGCAGGCTAAAGATAGTaa                                                                                                                                                                                            >  1:401310/1‑275 (MQ=255)
                                                                                               ggCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTCGTTTGCTGTACCGAAAAGAGCATTCCAGGAAAGTATCAATCGCAGTTCGACACCATCACTGGTCCACCGCAGCCCATAGATTGTCAGTGAACgaaa                                                                                                                                                                                  >  1:33630/1‑275 (MQ=255)
                                                                                                 cAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGTAGAATATGCATTAAACGGTTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGAGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCt                                                                                                                                                                              <  2:427446/277‑1 (MQ=255)
                                                                                                    aaatacgAAAAGGGATAGGACTCGCGAAACAGACATGAAAGAATATTATGCTTTATACGGCTAATCTACTCGTCTAAAGGATTAATCATGAAGTTGAATTTTAAGGTATTTTTTAAGGCTGCCTGTTTATTCCCACTGGCGCGGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAACGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAATGCTATGt                                                                                                                                                                          <  4:125270/272‑1 (MQ=255)
                                                                                                              aGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCGTTTCCCATACCGCAAAGGGAAGTTCAGGAAAGTCTCAATCGCAGTCAGACACCATCACTGGGCTATCGCATGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAggg                                                                                                                                                                >  2:508899/1‑278 (MQ=255)
                                                                                                                   tatCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGTTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACAACATTAATGGGCTATCGCAGGCAAAACACAGTCATGGAACAAAAGTCACTGTTTTTGAAgggtca                                                                                                                                                             >  2:337242/1‑273 (MQ=255)
                                                                                                                          cgcTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAAGGGAACAAAAGGCTATGTGTTTGTAGGGGAATCGCTgggt                                                                                                                                                    >  2:169938/1‑276 (MQ=255)
                                                                                                                           gcTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCACAGGGTAGTTCAGGACATTATAAATCTCAGTCAGACACCACACAGTTGCCACGGCAGGCATAATATAGTAATGGAACAAAAGGCTAGGTTGTTGTAGGGGACTGGGTGGAtg                                                                                                                                                   >  4:481207/1‑277 (MQ=255)
                                                                                                                            atAAACAGGAGGGAAATAGTAGTTTGTGTTGAAAGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAAGTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTTGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGAAACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAt                                                                                                                                                    <  4:132622/275‑1 (MQ=255)
                                                                                                                               aaCAGGGGGGAAAGAAGAGTATGCTTTCAACGGCTTTGCTATTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAt                                                                                                                                                    <  3:8171/273‑1 (MQ=255)
                                                                                                                               aaCAGGAGGGAAAGCATAGTTTGCTTTCCACGGCTTAGCTAATCGTTTAAAGGATTAATCGTGAAGTTGAATTTTAAGTGATTTTTTAAGTCTTCCTGTTTATTCCCACTGGCGCTGATGCTTTCAGGATGTATCTCGTATGCTATGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAt                                                                                                                                                    <  3:116120/273‑1 (MQ=255)
                                                                                                                                    gaggGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCNGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGATACCGCAGGCAAAAGATAGCAATGGAACACAAGGCTATGTTTTTGTAGTGGAATCGCTGTATCACCATATc                                                                                                                                           >  4:352531/1‑277 (MQ=255)
                                                                                                                                             aatgttatgtattCAACGGATTAGCTACTCGTTTAAAGGATTAATCATTAAGTTGAATTTTAAGGGATTTTTTAAGCCTGCCAGTTTATTCCCACTGGCGCTAATGCCTTCAAGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCGAAGGGTAGTTCAGGAAAAGATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATg                                                                                                                                    <  3:53697/264‑1 (MQ=255)
                                                                                                                                                    ttcTTTCAACTGCTGATGTACATGTTTAAAGGATTAGTCATGAAGGTTATATTATAGGGATTTTGGAAGGCTTCCTGTGTATTCCCACTGGCGCTGATGCTTTCAGGCTTTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACAACATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGa                                                                                                                          <  4:204567/276‑1 (MQ=255)
                                                                                                                                                     cctgtCAACGGCTTAGCTACTCGTGTAAAGGATTAACCATGATGTTTAATTTTATGGGATTGTTTAAGGCGGCCGTGTGACTCCCACTTGCTCTAATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGa                                                                                                                          <  4:169938/273‑1 (MQ=255)
                                                                                                                                                       tttCAACGTCTTAGCTACCGGTTTAAAGGAGTAAACTTGAAGTTGAATATTAAGTGATCTTTTACGCTGGCCGGTTTATATCCACTGGCTCTGATGCTTGCAGGCTGTATCTTGTATGCTCTGGTTGCCCATACCGCAAAGAGTAGTTCAGGAAAGTATCAAGCGCAGTCAGACACCATCACTGGGCTTTCGCAGGCAAAAGATAGTAATGGAACAAAAGGTTATGTTTTTGTAGGGGAATCGCTTGATTACCTTATCACTGATGGTGCCGATGa                                                                                                                          <  3:401310/275‑1 (MQ=255)
                                                                                                                                                        ttCAACTGTTTATGTAGTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAATGGATTTTTTAAGGCTTCAGGTATATTCCCACTGACGCTGATGCTGTCAGGCTGTATCGCGTATGCTCTGGTTTCCCATACCGGAAAGGGTAGTTAAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAATATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGa                                                                                                                          <  3:197259/274‑1 (MQ=255)
                                                                                                                                                            aCGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAACAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGACTCGCTGCATTACCTTCTTACTGTTGTTTCCGTAGACCTCGttt                                                                                                                  >  2:313753/1‑277 (MQ=255)
                                                                                                                                                              ggagtAGCTACTCGTTTAAAGGATTAAGAATGAAGTTGAATTTTAAGGGTTTTTTTAAGGCTGCCGGTTTAGTCCCACTGGCGCTGATGCTTACAGGATGTATCTCGTATGATCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACAATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAg                                                                                                                <  2:449485/274‑1 (MQ=255)
                                                                                                                                                              gcatgagtgatgcGTTTAAAGAATTATTCATGTAGATGAATTTTTAGGGATTTTTTGAAGCAGCCGGTTTAATCCCACTGGCGCTGATGATTTTAGGCTGTATCTCGAATGCTATGGTTTCCCAGACAACAAAGGGTAGTTCAGGAAAGTATCAATCGTAGTCAGACACAATAACTGGTCTATCGCAGGCAAAAGAAAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTAACTTATCACTGATGGTGCCGATGACATCGTTa                                                                                                                  <  4:372292/264‑1 (MQ=255)
                                                                                                                                                                         tCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCaa                                                                                                        <  3:458142/275‑1 (MQ=255)
                                                                                                                                                                                     ttAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGAACt                                                                                            >  1:78333/1‑275 (MQ=255)
                                                                                                                                                                                     ttAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGAGTGTGCCGTTGACATCGTTAAGATGCTCAATGTAACAGCACTTaa                                                                                         >  2:33265/1‑278 (MQ=255)
                                                                                                                                                                                      tAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTGCTTTAAAAAAAAGAGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAACAGATAGTAATGGAACAAAAGTCTATGTTTTTGTAGTGGCATCGCTGGATTACCTTATCACTGATGTTGCCGTTGACATCGTTAAGATGCTCAATTATCCAGCACTTAAc                                                                                        >  2:395781/1‑278 (MQ=255)
                                                                                                                                                                                         tCATGAAAGTGAATTTTAAGGTGTGTTTTAAGGCTGCCGGTTTATTCCAACTGGCGCGGATTCTTTCAGGCTGAATCTCTTATGATCTGGTTGCCGATACCGCAAAGGGTAGTTCAGGAAAGGATCAAACGCAGTCAGACACCATCACTGGGCTATCGAAGGCAAAAGATATTAATGGAACAAAAGGCTATGTTTTTGTAGGAGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAAc                                                                                        <  3:203792/275‑1 (MQ=255)
                                                                                                                                                                                              aaGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtttt                                                                                                                                                                       >  3:122024/1‑191 (MQ=255)
                                                                                                                                                                                                  tGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTa                                                                                                                                                                   <  1:122024/191‑1 (MQ=255)
                                                                                                                                                                                                         tAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCAAAATATTCAGGTTg                                                                     >  2:484416/1‑278 (MQ=255)
                                                                                                                                                                                                         tAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTGCCATAACGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCAAGCACTTAACCGGCACAATATTCAGGTTg                                                                     >  2:335102/1‑278 (MQ=255)
                                                                                                                                                                                                               aTTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATTCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAt                                                                                                                                                    <  2:397594/193‑1 (MQ=255)
                                                                                                                                                                                                                 tttttAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATg                                                                                                                           >  4:57844/1‑216 (MQ=255)
                                                                                                                                                                                                                  ttttAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGa                                                                                                                          <  2:57844/216‑1 (MQ=255)
                                                                                                                                                                                                                         gatcacttgtgtttCCCACTGGCGCTTAGGCTTTCAGTCTTTATCTCCTATGCTATTGTTGCCCCGACCGCACATGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGTGCGATCGCAGGCAAAAGATAGTAATGTAACCAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGAtt                                                     <  4:484416/266‑1 (MQ=255)
                                                                                                                                                                                                                          cTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTCGTTTCCTATACCACAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATCTTCAGGTTGCCGCTGACTCAATAttt                                                    >  2:458951/1‑278 (MQ=255)
                                                                                                                                                                                                                            gCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAg                                                                                                                                                                                                                                  >  4:169163/1‑103 (MQ=255)
                                                                                                                                                                                                                             ccGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTCTTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCTCTGGATTACCTTATCACTGATGGTGCCGATGACATc                                                                                                                      >  2:607298/1‑209 (MQ=255)
                                                                                                                                                                                                                             ccGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGa                                                                                                                                                                                                                                 <  2:169163/103‑1 (MQ=255)
                                                                                                                                                                                                                                     ctCCCACGCGCGCTGGTACTTTCTGTCTATGTCTCAGATTCACATGTGTCACATACCTTAAAGAGTAATTCATGAAAGTAACAATCGCAGTCAGACAACAGAACTGGGCCATCACAGGCAGAAGATAGTAATGGAAAAAAAGGATATGTTTTTGTAGGGGAATCACTGGATTACCTTATCACTGATGGTTCCGATGACATCGATAAAATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTaa                                             <  4:204122/273‑1 (MQ=255)
                                                                                                                                                                                                                                              gcgcTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCCATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTCTCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGACCCAGCACTTAACCGGCACAATATTCAGGTTCCCGCTGCAGCAAGCTTTGTTTTAAATGCGGGGaaaaa                                >  4:69762/1‑278 (MQ=255)
                                                                                                                                                                                                                                                           cAGGATGTATCTCGTATGCTATGGTTTCCCATACCGCAAAGGGTAGTTCATGAAAGTATCAATCGCAGTCAGACAACATCAATGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCgg                    <  2:352531/277‑1 (MQ=255)
                                                                                                                                                                                                                                                                    tctcGTATGCTATGGTTTCCCAGGCCGCGAAGGGTAGTTGAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAata             <  1:59617/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTGTTTGTAGGGGAATCGCTGGATTAACTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGCTGCCGATGACGCAAGATTTGTTATAAATTCGGGGGAAAAGAAATTAACCGGCaaaaacacggttt      >  4:102678/1‑269 (MQ=255)
                                                                                                                                                                                                                                                                            gCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTAAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACAGGCACAATATCGCtttccgt  >  4:105527/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                    
GCGTTTGCCGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTA  >  NC_000913/3875251‑3875796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: