Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,145,313 | C→A | S683Y (TCC→TAC) | yegE → | putative diguanylate cyclase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,145,313 | 0 | C | A | 98.4% | 451.7 / NA | 124 | S683Y (TCC→TAC) | yegE | putative diguanylate cyclase |
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (65/57); total (66/58) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 124 total aligned reads displayed.
GAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGACTC‑GGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAT > NC_000913/2145045‑2145584 | gaagCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATGGGTTCGGTTCTGGTGATTCAGGACGGTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatg > 1:639884/1‑274 (MQ=255) tCCTGACGGTGTTGCATATTACTTTTTGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAAc < 2:574773/278‑1 (MQ=255) cTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCaa > 1:601949/1‑275 (MQ=255) tGACGGGGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAAc < 1:89190/275‑1 (MQ=255) actgtgtTGCATATTACTTGTGACGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCgcgc < 2:468537/275‑1 (MQ=255) ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATTATGCCATTACGCATCCCGCCAACCGCCCCAGTTTTGaaaac > 3:670806/1‑271 (MQ=255) ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGATGAGCTACACCGCCTACCATGATCCAATGACCCATCCCGACAACCGCGCCAGTTTTGAGCaacaacgt > 4:625666/1‑276 (MQ=255) ggCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCCGTAAGATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTCCAGCCCCTAACCTGATGCACTGACGCATCTCGCCAAACGCGCCATGTTTGACAaaaaac > 3:607408/1‑274 (MQ=255) ggCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCACACCGCGCCAGTTTTGGGaaaacact > 3:693610/1‑270 (MQ=255) cGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATcc > 3:381707/1‑275 (MQ=255) attacggagaaaaaTTTACAGTGCCGACACCGCACGTTCCGCGTTCGAGCAAGATTTGGTGTTGCACGGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATAGTGATTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCtgctgc < 1:568578/262‑1 (MQ=255) aTTAATGGAGAACATTTACAGTGCCGAAACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCtgctgc < 1:588588/275‑1 (MQ=255) tGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg < 3:576604/275‑1 (MQ=255) agcaaaTTTACAGTGCCGAAAACGCACGTCTGGCGATCGAGCAAGATGTTGTGTTGCCCTGTCGGAGCGGCGGCTGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa < 2:602729/272‑1 (MQ=255) gctattACAGTGCCGACGCCACCCGTTCCGCGATCGAGCAAGATGTGGTGTTGCACGGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa < 3:107824/270‑1 (MQ=255) cATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGCATCCTGCTGCAAACGGTAAACa > 1:107824/1‑275 (MQ=255) cATTGACAGTGCCGTCACCCCCCGTTCCGCTATCGAGCAAGTTGGTGTGTTGCACTGTCGGAGCGGCGTCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTGAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa < 4:260016/278‑1 (MQ=255) gCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAAGCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAACCAACTGCGACGCCTGATGCAAACGGCACACAGTACACATAAg > 2:277431/1‑276 (MQ=255) ccGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGCGCTACAGCGCCTACCATGATGCACTGACGCCTCCCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGAAAACGGTAAACATTACACAAAAGCGa > 4:585224/1‑278 (MQ=255) ccGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgag > 2:267824/1‑230 (MQ=255) cGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc < 2:585224/278‑1 (MQ=255) cacCTCCCGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc < 3:442320/275‑1 (MQ=255) cacCTCACGTTCCGCGATCGAGCAAGATCTGGTGTTGCACTGTCGGAGCGCCGGCAGTTACGACGTGCATTAAAGTATTCCACCGTTAAGTACTCTGGACGGCCGCACTACTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCGGCGCTACAGCGCCTACCTTGATGCACTGCCGCACCTCGCCAACCGCGCACGCTTTGAGCATCCATACCGTACCCTGCTGTCAATGGTCAATAGCCCACTCCAGCGAc > 3:176501/1‑275 (MQ=255) acCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACa < 1:607816/275‑1 (MQ=255) tCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCt > 2:491428/1‑278 (MQ=255) cACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc < 1:381707/275‑1 (MQ=255) cACGTTCCGCAATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTGTTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAACTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc < 1:407348/275‑1 (MQ=255) tCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGTACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTCCTGCAAACGGTAAACAGTACACATCAGCAGCATGCCCTGGTGtt > 2:439147/1‑278 (MQ=255) ccGCGATCGAGCAAGAGGTGGTGTTGCACGGTCGGGGCGGCGGCAGTTACGACGTGCATTACAGTATCACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGtt < 2:430835/277‑1 (MQ=255) gcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAACCCACTGCGTATCCTGCTTCAAACGGTAAACAGTACACATCAGCAACCTGCCCTGGTGTTTa > 2:595313/1‑277 (MQ=255) gcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGtt > 1:197706/1‑275 (MQ=255) aTGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑At < 1:533240/275‑1 (MQ=255) gtgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAACCAACTGCGTATCCtgctgc < 1:224711/217‑1 (MQ=255) tgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATATTG‑ATAGCttt > 1:550949/1‑275 (MQ=255) ttGCCCGGTCGGGGCGGCGGCAGTTACGAGGTGCATTAAGGTATTCAGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTGCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAg < 4:472356/277‑1 (MQ=255) ttGCACTGGCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAg < 2:291667/277‑1 (MQ=255) tGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTaa > 1:618940/1‑274 (MQ=255) tGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCCACCATGATGCACTGCAGCACCTCGCCAACCGCGCCAGTTTTTAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTATACATCAGCCACATCCCCTGGTTCTTCTCGATCTTG‑ACTGATTTCAAGca > 4:246246/1‑277 (MQ=255) cACTGTCGGGGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAg < 1:346410/274‑1 (MQ=255) gcggcgGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTTTCCTGCTGCAAACGGTAAACAGTACACATCCGCGAACTGCCCTGGTGTTTATCGCTCTTG‑ACTGCTTTATACGGGTGAATGaa > 3:220717/1‑274 (MQ=255) gcggcgGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCGGGTGTTTATCGACCTTT‑ATCCCTTTAAAGCGGGGaaggcc > 3:547777/1‑271 (MQ=255) ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCACCCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAgcgc > 3:504957/1‑275 (MQ=255) ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAACCAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGAGGTGACTGACAgcgc > 1:495194/1‑275 (MQ=255) ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGGTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTCAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑AACGCTTTAATGCGGTGAAGGACAGcggcgg > 2:428446/1‑278 (MQ=255) tGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACa > 1:330759/1‑211 (MQ=255) tcATTACAGTAGTACGACGTTGAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTg < 2:485199/277‑1 (MQ=255) ttACAGTATTACGCCGTTAAGGACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc < 3:330759/211‑1 (MQ=255) cAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTg < 1:659867/272‑1 (MQ=255) cAGTATTACGCCGTTAAGTACCCTGTACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTg < 1:329413/272‑1 (MQ=255) tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATgcgg > 3:121215/1‑263 (MQ=255) ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGTTGCCGCTTtg > 1:157323/1‑275 (MQ=255) ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCAGGG‑CATGCGGCGGGTTACGCGTtg > 3:144656/1‑275 (MQ=255) ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGAGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACTCATCTCGCCAACCGCGCCAGTTTTTAGAAAAAACTGAGTATCATGCTGACACCGGGAAACAGCAAACAGACGACGCATGACCTGGTGTTTATCGATATTG‑CTCGCTTTAAAGCGGTTAATGACAACGCCGGG‑TACGCGGCGGGTCAGGCtcttct > 4:208241/1‑272 (MQ=255) aCGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCggg < 1:121215/263‑1 (MQ=255) aCGCCGTTAAGTACCCTTGCCGACAGCATTATTGTTGCGGTTAGCTCGATTCAGGACTGTACCGAATCACGCAACATGCTGGGCAAGCTGAGCTACAGCGCCTACCATGATGGACTGACGCATCTCGCCAACCGCGACAGTTTTGCGAAAAAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTgcg < 4:263014/278‑1 (MQ=255) cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAAc > 4:330639/1‑172 (MQ=255) cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCt > 1:567749/1‑217 (MQ=255) cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTtgctg > 1:210727/1‑275 (MQ=255) gCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg < 2:330639/172‑1 (MQ=255) ccGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGCGCTACAGCGCCTACCATGATGAACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCACACGGTCAACAGTACACATCAGCGACCTGCACTGGTGTTTATCGATCTTG‑ATCGCTTTCACGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCtttcacgcccga > 2:138531/1‑269 (MQ=255) cGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGCTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGAGACATGCCCTGGTGTTCAGCCCTCTTGCATC‑ATTTAAAGCGGTGAAGTGCAGCGACGGGCCTTGGGGCGGTTG‑CGCCTtgctgctc > 3:603132/1‑273 (MQ=255) gTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑At < 3:567749/217‑1 (MQ=255) gTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGcgcg < 4:393403/275‑1 (MQ=255) gTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGcgcg > 2:393403/1‑275 (MQ=255) ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCt > 1:454615/1‑212 (MQ=255) ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGa > 1:489852/1‑275 (MQ=255) aaGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCCAACGGTAACCAGTACACATCAGCGAAATGCCACGGTGTTTATCGATCTTG‑AACGCTTTAAAGCGGTTAATGCCAGCTCCGGG‑CCGGCGGCAGGTGAGGCTTGGCTgcgccgaatgg > 4:273375/1‑271 (MQ=255) gTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑At < 3:454615/212‑1 (MQ=255) acttgtaaCGGGAGCAGTGTAGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCGGCTGAGCTACGCCGCCTCCCATGATGCACTGACGCATCTAGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTCTCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc < 3:601949/268‑1 (MQ=255) aCTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc < 1:547777/275‑1 (MQ=255) tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACCCATCAGCGACATGCCCTGGTGTTTCTCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGAGCCGGG‑CATGCGGCGGGTGCCGCTGTGCCGCGCGAACTGGcgcctt > 4:247029/1‑274 (MQ=255) tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGcgtcg > 1:538772/1‑275 (MQ=255) tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGGGAATGACAGCGCCGGG‑CCTGCGGCGGGTGCGGCTTTCCTGCGCGAACTGGcgtcg > 1:316616/1‑275 (MQ=255) gACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACCCATCAGCGACATGCCCTGGTGTTTAATGATCTTG‑ATCGCTTTAAAGCGGTGAATGCCAGCGCCGGG‑CCGGGGGCGGGTGAAGCTTTCTTGCGTGAACTGGcggtgg > 3:338047/1‑270 (MQ=255) cagcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGc > 1:151554/1‑275 (MQ=255) atatTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAgc > 1:375373/1‑218 (MQ=255) tGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCg < 3:375373/218‑1 (MQ=255) ggttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTAtgct > 1:439325/1‑275 (MQ=255) gttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTAtgct < 3:538772/274‑1 (MQ=255) gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGATGAATGACAGGGCAGGG‑CACGCGGCGGGTGACGCTTTGCTGCGCGCACTGGCCTCGTGAATGATGCGTACTCTGCGTTc > 3:145483/1‑275 (MQ=255) gttGATTCAGGGCGGTACCGAATCACGCAAAATGCTGCGCCAGCTGAGATACCGCGCCTACCATGAGGCACTGGCGCATCTGGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCTGCGAAATGCCCTGGTGGTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCg < 3:639884/273‑1 (MQ=255) gTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCTGCGTTTATGCTGGGTATGCTGCGTTCCAggc > 3:50997/1‑272 (MQ=255) tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTgg > 2:108989/1‑278 (MQ=255) tACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGGCGTTAATGCTGGGGATGCTGCGGTCCAGCGAC‑GGCGCGGCGCGAc > 3:69881/1‑275 (MQ=255) aCCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTTCGTATGCTGCGTTCCTGCGGC‑GTTCTGGCGCGCCt > 3:644644/1‑275 (MQ=255) gcataaCGGAAAATGCGGCCGCCGCTGGGCTACAGCACGTACGATGATGCACTGACGAATCTCGCCAAACGCTCCAGTTTGTAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGATGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGACTC‑g < 3:273877/269‑1 (MQ=255) aaTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGGGCGAACTGGCGTCGGTAATGCTGAGTATGCTGGGTTCCGGCGGCTGTG‑TGGCGCGAGTC‑GGTggg > 4:537523/1‑278 (MQ=255) cACGCAAAATGCTGCGACAGGTGGGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAAGGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGACTC‑GGTGGGGa < 2:35227/277‑1 (MQ=255) cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAg > 1:100687/1‑123 (MQ=255) aaaTGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc < 3:100687/123‑1 (MQ=255) tgccacAGCTTGGCTACAGCGCCAACCATGATTACCTGACGCATCTCGCCAACCGTGCCAGTTTTGGGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGGGTTTATCGATCTTG‑ATCGCTTTAAGGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGACTC‑GGTGGGGATGAATTTGGt < 3:653460/270‑1 (MQ=255) gcgcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCTGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGGGTATGCGGCTTTCCAGCGAC‑GTGCTGGGCCGACTCGGGTggggggatttatgtgt > 4:13579/1‑264 (MQ=255) gcgcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGGGAC‑GTTCTGGCGCGACTC‑GGTGGGGGTGGATTTgg > 3:328042/1‑273 (MQ=255) gcgcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGTCTC‑GGTGGGGGTGAATTTGGTct > 2:559647/1‑276 (MQ=255) gCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGACTC‑GGTGGGGATGAATTTGGGCTGCTGCTAc > 2:491319/1‑278 (MQ=255) aGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGGCCC‑GGTGGGGAGGGATTTGGTCTTCTGCTACCGGa > 4:635576/1‑278 (MQ=255) gCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGACTC‑GGTGGGGATGAATTTGGTCTGCTGCTACCa < 1:48815/275‑1 (MQ=255) cTACAGCGCCTACCATGATGCACTGACGCGTCTAGCCAACGGGGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCGTTGCTGCGCGAACTGGCGTCGTTAATGCAGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCAACTC‑GGTGGGGATGAATTTGGTCTGCTGCTACCa < 1:176501/274‑1 (MQ=255) cAGCGCCTACCATGATCCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGACTC‑GGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGt < 2:93269/277‑1 (MQ=255) ccTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGACTT‑GGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAt > 1:559019/1‑275 (MQ=255) | GAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTG‑ATCGCTTTAAAGCGGTGAATGACAGCGCCGGG‑CATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGAC‑GTGCTGGCGCGACTC‑GGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAT > NC_000913/2145045‑2145584 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |