Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,164,285:1 +T 6.0% coding (1689/2259 nt) parC ← DNA topoisomerase IV, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,164,2851.T 6.0% 347.6 / 17.2 83coding (1689/2259 nt)parCDNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base . (40/38);  new base T (2/3);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

CCACCAGATCGTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATGGCC  >  NC_000913/3164137‑3164431
                                                                                                                                                     |                                                                                                                                                  
ccaccaGATCGTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCAcgc                                                                                                                                                     >  3:81537/1‑149 (MQ=255)
 caccaGATCGTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCAcgc‑g                                                                                                                                                   >  1:718164/1‑149 (MQ=255)
    caGATCGTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCg                                                                                                                                                >  4:245375/1‑149 (MQ=255)
     aGATCGTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGa                                                                                                                                               >  2:505980/1‑149 (MQ=255)
          gTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCa                                                                                                                                          <  1:516680/149‑1 (MQ=255)
          gTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCa                                                                                                                                          >  4:590204/1‑149 (MQ=255)
          gTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCa                                                                                                                                          <  1:838356/149‑1 (MQ=255)
          gTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCa                                                                                                                                          >  4:119932/1‑149 (MQ=255)
          gTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCa                                                                                                                                          >  1:291743/1‑149 (MQ=255)
           ttAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAg                                                                                                                                         >  2:155868/1‑149 (MQ=255)
            tAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGc                                                                                                                                        <  3:164419/149‑1 (MQ=255)
               aGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCTGCCGTCGGcgcgtg                                                                                                                                      <  4:694235/149‑6 (MQ=255)
                    cAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCgg                                                                                                                                <  1:102959/149‑1 (MQ=255)
                     aGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCggg                                                                                                                               <  1:222986/149‑1 (MQ=255)
                         gAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCaa                                                                                                                           <  2:395184/149‑1 (MQ=255)
                          aaaCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAAt                                                                                                                          <  1:271560/149‑1 (MQ=255)
                          aaaCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCAACGGCAGCGTAATCGGGACAAt                                                                                                                          <  4:587365/149‑1 (MQ=255)
                           aaCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATg                                                                                                                         <  4:854515/149‑1 (MQ=255)
                            aCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCTGCCGTCGGcgcgtgg                                                                                                                                     <  3:106008/137‑7 (MQ=255)
                            aCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCTGCCGTCGGcgcgtgg                                                                                                                                     >  4:106008/1‑131 (MQ=255)
                            aCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATgg                                                                                                                        >  3:847283/1‑149 (MQ=255)
                            aCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATgg                                                                                                                        <  2:388734/149‑1 (MQ=255)
                             ccGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGc                                                                                                                       >  2:224920/1‑149 (MQ=255)
                                  aCCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGc                                                                                                                  <  1:226977/149‑1 (MQ=255)
                                    ccGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCTGCCGTCGGcgcgtg                                                                                                                                      >  3:364931/1‑123 (MQ=255)
                                    ccGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCTGCCGTCGGcgcgtg                                                                                                                                      <  4:364931/128‑6 (MQ=255)
                                     cGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTAc                                                                                                               <  3:317907/149‑1 (MQ=255)
                                     cGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTAc                                                                                                               <  2:875956/149‑1 (MQ=255)
                                           ggAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGCCCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCgg                                                                                                         >  4:109919/1‑149 (MQ=255)
                                                  aTCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATc                                                                                                  <  2:883451/149‑1 (MQ=255)
                                                  aTCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATc                                                                                                  >  4:147425/1‑149 (MQ=255)
                                                   tCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCATCGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATca                                                                                                 >  3:576574/1‑149 (MQ=255)
                                                    cagcagTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATcaa                                                                                                <  2:533984/149‑1 (MQ=255)
                                                    cagcagTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAACcaa                                                                                                <  2:143538/149‑1 (MQ=255)
                                                        agTTTCTGATCGTCGCTTTCCAACAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCaacaaa                                                                                            <  2:789233/149‑1 (MQ=255)
                                                          tttCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCaacaaaca                                                                                          >  1:475944/1‑149 (MQ=255)
                                                           ttCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAacac                                                                                         <  2:718164/149‑1 (MQ=255)
                                                            tCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACt                                                                                        >  1:95319/1‑149 (MQ=255)
                                                              tGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTAc                                                                                      >  3:358010/1‑149 (MQ=255)
                                                                aTCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCg                                                                                    >  4:482097/1‑149 (MQ=255)
                                                                aTCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCg                                                                                    <  3:727715/149‑1 (MQ=255)
                                                                  cgtcgCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCgg                                                                                                                                >  1:881321/1‑103 (MQ=255)
                                                                  cgtcgCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCgg                                                                                                                                <  2:881321/103‑1 (MQ=255)
                                                                    tcgCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCggttg                                                                                >  3:195454/1‑149 (MQ=255)
                                                                     cgCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCggttgg                                                                               <  2:95319/149‑1 (MQ=255)
                                                                     cgCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCggttgg                                                                               <  3:245375/149‑1 (MQ=255)
                                                                        tttCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGgttggttg                                                                            >  4:263850/1‑149 (MQ=255)
                                                                        tttCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGgttggttg                                                                            >  4:371842/1‑149 (MQ=255)
                                                                        tttCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTCAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGgttggttg                                                                            >  4:625523/1‑149 (MQ=255)
                                                                         ttCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGc                                                                           >  3:274738/1‑149 (MQ=255)
                                                                          tCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGct                                                                          >  2:756068/1‑149 (MQ=255)
                                                                           ccATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGctc                                                                         <  3:521724/149‑1 (MQ=255)
                                                                             aTCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGTCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCtt                                                                       >  3:508501/1‑149 (MQ=255)
                                                                             aTCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCtt                                                                       >  1:766949/1‑149 (MQ=255)
                                                                             aTCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCtt                                                                       >  1:67889/1‑149 (MQ=255)
                                                                             aTCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCtt                                                                       >  2:727432/1‑149 (MQ=255)
                                                                               cAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTAc                                                                     >  1:763973/1‑149 (MQ=255)
                                                                               cAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTCAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTAc                                                                     <  3:625523/149‑1 (MQ=255)
                                                                                  cATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATGTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCtt                                                                  <  4:546426/149‑1 (MQ=255)
                                                                                    tatGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTc                                                                >  3:719082/1‑149 (MQ=255)
                                                                                      tGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAc                                                              <  3:371842/149‑1 (MQ=255)
                                                                                       ggTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAcc                                                             >  2:670916/1‑149 (MQ=255)
                                                                                               gTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCttt                                                     <  4:274738/149‑1 (MQ=255)
                                                                                               gTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCttt                                                     <  3:162972/149‑1 (MQ=255)
                                                                                                  gCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGaa                                                  <  3:590204/149‑1 (MQ=255)
                                                                                                  gCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGaa                                                  <  4:261475/149‑1 (MQ=255)
                                                                                                    cccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGc                                                >  2:363200/1‑149 (MQ=255)
                                                                                                     ccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCt                                               >  2:791477/1‑149 (MQ=255)
                                                                                                      cAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTa                                              <  3:665281/149‑1 (MQ=255)
                                                                                                        ggcggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATc                                            >  2:288917/1‑149 (MQ=255)
                                                                                                          cggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCAc                                          >  4:751927/1‑149 (MQ=255)
                                                                                                            gcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCAccc                                        <  1:852328/149‑1 (MQ=255)
                                                                                                              aaCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCAGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGc                                      >  2:732548/1‑149 (MQ=255)
                                                                                                                     aTTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATaa                               <  3:8001/149‑1 (MQ=255)
                                                                                                                      tttGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAt                              >  2:727241/1‑149 (MQ=255)
                                                                                                                       ttGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAtt                             <  1:505980/149‑1 (MQ=255)
                                                                                                                         gCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGTTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCa                           <  4:81537/149‑1 (MQ=255)
                                                                                                                                    gCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGcgcg                >  4:262975/1‑149 (MQ=255)
                                                                                                                                     cTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGt               >  1:375215/1‑149 (MQ=255)
                                                                                                                                          ccTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTAc                                                                                      >  2:179394/1‑73 (MQ=255)
                                                                                                                                          ccTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTAc                                                                                      <  1:179394/73‑1 (MQ=255)
                                                                                                                                          ccTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGata          >  4:346097/1‑149 (MQ=255)
                                                                                                                                              aCCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCAt      >  2:549345/1‑149 (MQ=255)
                                                                                                                                               ccACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATg     <  3:119932/149‑1 (MQ=255)
                                                                                                                                                 aCGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATGGc   <  3:482097/149‑1 (MQ=255)
                                                                                                                                                  cgc‑gCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAACCACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATGGcc  <  3:819016/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCACCAGATCGTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATGGCC  >  NC_000913/3164137‑3164431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑