Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,169,265:1 | +T | 5.0% | intergenic (‑34/+19) | ygiV ← / ← ygiW | transcriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,169,265 | 1 | . | T | 5.0% | 513.7 / 21.6 | 120 | intergenic (‑34/+19) | ygiV/ygiW | transcriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein |
Reads supporting (aligned to +/- strand): ref base . (60/54); new base T (4/2); total (64/56) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.84e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01 |
Only 100 of 124 total aligned reads displayed.
GATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATA > NC_000913/3169118‑3169413 | gATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑t < 4:687799/149‑1 (MQ=255) aaTTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCg > 4:460344/1‑149 (MQ=255) aTTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGTGGCAACATCGCCACAGGTATTGATGTGAAATCGTTAATGTTTACATCCAATGTCAGGTTTGTCATTTTGCCTCCCTGCGCAATGATTCCCGGGAACGCG‑TCCggg < 4:548593/149‑1 (MQ=255) aTTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCgg > 4:807905/1‑149 (MQ=255) tttCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCggg > 2:232083/1‑149 (MQ=255) ttCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGG‑ACGCGTTCCCGGGa < 4:351456/149‑1 (MQ=255) gCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCg > 2:310535/1‑149 (MQ=255) gCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCg > 4:582992/1‑149 (MQ=255) ccACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCgg > 2:152578/1‑149 (MQ=255) cACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGt > 3:285722/1‑149 (MQ=255) cGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTaa < 1:232083/149‑1 (MQ=255) tGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACaa > 1:754288/1‑149 (MQ=255) tAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACGGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAAtt > 3:876898/1‑149 (MQ=255) gTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTAc < 1:314471/149‑1 (MQ=255) gTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTAc < 1:704920/149‑1 (MQ=255) ttGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACg > 2:559235/1‑149 (MQ=255) gAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGa > 1:256786/1‑149 (MQ=255) gAGCAATTCAGGGCGACAGCGGTGCGGCAACATCGCCACAGGTCATGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGTACCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGa < 2:68648/149‑1 (MQ=255) gCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGTAtt < 1:68133/149‑1 (MQ=255) gCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGAtt < 3:460344/149‑1 (MQ=255) gCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGAtt > 4:150890/1‑149 (MQ=255) aaTTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTa > 2:403795/1‑149 (MQ=255) cAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACttt < 1:663825/149‑1 (MQ=255) aGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTa > 3:68834/1‑136 (MQ=255) aggCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAAACAATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGTTTTACttttc > 2:144492/2‑147 (MQ=255) ggCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCg < 4:273501/149‑1 (MQ=255) cGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGtt < 1:242575/149‑1 (MQ=255) ggTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGttt < 3:37410/149‑1 (MQ=255) tGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGa < 1:504837/149‑1 (MQ=255) tGCCGCAACATCGCCACAGGTATTGATAGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGAGAAATTATTCCCGGGAACGCG‑TCGCGGGAGCGGTAGCTATTACGGATTTACTTTGCGGATCAGTTTGa < 4:171958/149‑1 (MQ=255) gCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGAc < 4:130829/149‑1 (MQ=255) aaCATCGCCACAGGTATTGATGGGGAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCaa < 2:583844/149‑1 (MQ=255) aaCATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCaa > 2:262322/1‑149 (MQ=255) aCATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAAt < 2:58432/149‑1 (MQ=255) aTCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAAttt < 3:807905/149‑1 (MQ=255) aTCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAAttt > 3:347755/1‑149 (MQ=255) tCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTc < 2:782915/149‑1 (MQ=255) tCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTc < 2:108499/149‑1 (MQ=255) gCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCaa < 2:645911/149‑1 (MQ=255) gCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCaa < 1:856611/149‑1 (MQ=255) gCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCaa > 3:842057/1‑149 (MQ=255) ccACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATCCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAAc < 3:377338/149‑1 (MQ=255) acaGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAg > 2:438292/1‑149 (MQ=255) acaGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAg > 1:493366/1‑149 (MQ=255) aGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGaa < 2:256786/149‑1 (MQ=255) tATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGaa > 2:696232/1‑146 (MQ=255) ttGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCa < 1:310535/149‑1 (MQ=255) ttGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCa > 1:124455/1‑149 (MQ=255) aTGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGAt < 4:200261/116‑1 (MQ=255) aTGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGAt > 3:200261/1‑116 (MQ=255) tGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTAGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGTATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCt > 4:744664/1‑149 (MQ=255) gggAAATCGATAATGTTTACATCCAGTGTGAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCtt < 4:792688/149‑1 (MQ=255) gggAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACttt < 4:856584/108‑1 (MQ=255) gggAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACttt > 3:856584/1‑108 (MQ=255) ggAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCttt > 3:62619/1‑149 (MQ=255) cGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGAc < 3:114702/149‑1 (MQ=255) gATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACt > 2:563975/1‑149 (MQ=255) gATAATGTTTACATCCAGGGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACt > 4:837698/1‑149 (MQ=255) aTAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGc < 3:678060/101‑1 (MQ=255) aTAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGc > 4:678060/1‑101 (MQ=255) aTAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACtt > 2:720535/1‑149 (MQ=255) tAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTc > 3:633466/1‑149 (MQ=255) aTGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCAc < 4:876898/149‑1 (MQ=255) gTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCa > 4:326310/1‑130 (MQ=255) gTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCa < 3:326310/130‑1 (MQ=255) tCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAAtct > 4:504289/1‑149 (MQ=255) tCCAGTGTCAGGTTTGTCAGTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATATCTACAGATTTTCAGTCTTTATCGACTTCACCCTGAAtct > 2:240557/1‑149 (MQ=255) aGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCGGGGAGCGGTAACAATTACGGATTTACTTTGCGCATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCaa < 4:842057/149‑1 (MQ=255) gtgtCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAAc < 4:347755/149‑1 (MQ=255) tCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTAc > 1:304646/1‑69 (MQ=255) tCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTAc < 2:304646/69‑1 (MQ=255) aGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTAt > 4:160247/1‑149 (MQ=255) gTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCt > 3:259866/1‑149 (MQ=255) gTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCt > 1:875011/1‑149 (MQ=255) tttGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCtt > 3:29493/1‑149 (MQ=255) ttGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCttt > 3:515023/1‑149 (MQ=255) tGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCaa < 4:366114/99‑1 (MQ=255) cATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGc < 1:144492/149‑1 (MQ=255) cATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAACTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGc < 1:659724/149‑1 (MQ=255) cTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGcgtcaccgt > 3:431633/1‑149 (MQ=255) cTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGcgtcaccgt > 2:432437/1‑149 (MQ=255) tCCCGGCAAAGTTATTCCCGGGTACGCG‑TCCAGGGAGCGGTAACAATTACGCATCGGCTTTGTGTGTCTGTTTGAAGCCAATTTCAACAGAATTGCAGGCTTTCTCGACTTCACCCAGGATTTCCACCATATCTTTCGTcgtcaccgtc > 4:264459/1‑149 (MQ=255) ccTGCGAAATTCTTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCtt > 3:499230/1‑100 (MQ=255) ccTGCGAAATTATTCCTGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCtt < 4:499230/100‑1 (MQ=255) aaaTTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTGTCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGtt > 1:632345/1‑149 (MQ=255) attattCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTcc < 4:29493/149‑1 (MQ=255) attCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGc < 2:190995/149‑1 (MQ=255) ctCCCGGG‑ACGCGTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCg > 3:404189/2‑149 (MQ=255) tCCCGGG‑ACGCGTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGt > 4:244789/1‑149 (MQ=255) tCCCGGG‑ACGCGTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGt > 1:482863/1‑149 (MQ=255) tCCCGGG‑ACGCGTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGt > 1:388929/1‑149 (MQ=255) ccGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGttt < 4:724717/149‑1 (MQ=255) ggAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTtgtg > 2:82072/1‑149 (MQ=255) aaCGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGt < 1:720535/149‑1 (MQ=255) aCGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACTTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTc > 2:500862/1‑149 (MQ=255) aCGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGt < 4:567383/107‑1 (MQ=255) aCGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGt > 3:567383/1‑107 (MQ=255) aCGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTc < 1:559235/149‑1 (MQ=255) gcg‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGa > 1:727468/1‑149 (MQ=255) g‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGata > 1:820106/1‑149 (MQ=255) | GATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCG‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATA > NC_000913/3169118‑3169413 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |