Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 710,170 C→T 9.7% intergenic (+54/+30) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913710,1700CT 9.7% 237.9 / 12.3 81intergenic (+54/+30)chiQ/furchitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base T (4/4);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

AGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCACGCTGA  >  NC_000913/710025‑710317
                                                                                                                                                 |                                                                                                                                                   
agTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCa                                                                                                                                                  <  1:392728/149‑1 (MQ=255)
      tGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTgg                                                                                                                                            <  3:158054/149‑1 (MQ=255)
       gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGc                                                                                                                                           <  2:850907/149‑1 (MQ=255)
       gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGc                                                                                                                                           <  4:667268/149‑1 (MQ=255)
        cGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCt                                                                                                                                          <  2:160649/149‑1 (MQ=255)
           gCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtttt                                                                                                                                       <  4:201640/149‑1 (MQ=255)
            cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                                                                      >  1:168887/1‑149 (MQ=255)
            cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                                                                      <  4:640703/149‑1 (MQ=255)
            cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                                                                      <  1:599208/149‑1 (MQ=255)
            cAACGAAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                                                                      <  4:442264/149‑1 (MQ=255)
              aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtctc                                                                                                                                    <  3:436847/149‑1 (MQ=255)
                gCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGt                                                                                                                                  >  3:538617/1‑149 (MQ=255)
                   aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttt                                                                                                                                        <  1:607446/140‑1 (MQ=255)
                   aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttt                                                                                                                                        >  2:607446/1‑140 (MQ=255)
                   aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttt                                                                                                                                       <  4:496683/141‑1 (MQ=255)
                   aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttt                                                                                                                                       >  3:496683/1‑141 (MQ=255)
                    aCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAg                                                                                                                              >  1:404495/1‑149 (MQ=255)
                        gAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctg                                                                                                                          >  1:636200/1‑149 (MQ=255)
                        gAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctg                                                                                                                          <  1:306982/149‑1 (MQ=255)
                                 aGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAt                                                                                                                    >  1:63662/1‑146 (MQ=255)
                                 aGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAt                                                                                                                    <  2:63662/146‑1 (MQ=255)
                                 aGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTg                                                                                                                 >  2:652572/1‑149 (MQ=255)
                                 aGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTg                                                                                                                 >  2:207490/1‑149 (MQ=255)
                                    cGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCt                                                                                                              <  1:865341/149‑1 (MQ=255)
                                        gAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGt                                                                                                          <  4:567647/149‑1 (MQ=255)
                                          aaGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgc                                                                                                        >  3:838611/1‑149 (MQ=255)
                                           aGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGTCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcg                                                                                                       >  4:555154/1‑149 (MQ=255)
                                               gATTGTGATACAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTCGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATg                                                                                                   >  2:87206/1‑149 (MQ=255)
                                               gATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATg                                                                                                   >  1:361101/1‑149 (MQ=255)
                                                aTTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGt                                                                                                  >  1:870802/1‑149 (MQ=255)
                                                        aaaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTc                                                                                          >  4:408231/1‑149 (MQ=255)
                                                            tCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGGGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAACAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCgg                                                                                      >  4:257879/1‑149 (MQ=255)
                                                            tCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCgg                                                                                      >  3:164079/1‑149 (MQ=255)
                                                                 cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATc                                                                                 <  2:780297/149‑1 (MQ=255)
                                                                  aGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCg                                                                                >  3:372546/1‑149 (MQ=255)
                                                                     aaaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCt                                                                             <  2:168887/149‑1 (MQ=255)
                                                                      aaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCtt                                                                            <  4:164079/149‑1 (MQ=255)
                                                                         aCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgc                                                                                                        >  3:631454/1‑118 (MQ=255)
                                                                         aCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgc                                                                                                        <  4:631454/118‑1 (MQ=255)
                                                                           gCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                                                                               <  3:398536/141‑1 (MQ=255)
                                                                           gCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                                                                               >  4:398536/1‑141 (MQ=255)
                                                                           gCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGca                                                                       >  3:810946/1‑149 (MQ=255)
                                                                               taataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCtt                                                                                                             <  2:25923/107‑1 (MQ=255)
                                                                               taataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCtt                                                                                                             >  1:25923/1‑107 (MQ=255)
                                                                                 ataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                                                                 <  2:616659/149‑1 (MQ=255)
                                                                                           aGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATGTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGAt                                                       >  4:607107/1‑149 (MQ=255)
                                                                                             tGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTCCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATag                                                     <  1:253181/149‑1 (MQ=255)
                                                                                              gagaGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATaga                                                    <  3:566110/149‑1 (MQ=255)
                                                                                                gagCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga                                                  <  4:820797/149‑1 (MQ=255)
                                                                                                gagCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga                                                  <  3:408231/149‑1 (MQ=255)
                                                                                                     gTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtgtg                                             <  4:737212/149‑1 (MQ=255)
                                                                                                          tctcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAACGATAGAGACTGTGGTTAg                                        >  4:741923/1‑149 (MQ=255)
                                                                                                          tctcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAg                                        >  1:376742/1‑149 (MQ=255)
                                                                                                          tctcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAg                                        >  3:746144/1‑149 (MQ=255)
                                                                                                           ctcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGt                                       <  3:158727/149‑1 (MQ=255)
                                                                                                              gCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAg                                    <  3:555154/149‑1 (MQ=255)
                                                                                                               cTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAgg                                   <  4:372546/149‑1 (MQ=255)
                                                                                                                ttttCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGc                                  >  3:590495/1‑149 (MQ=255)
                                                                                                                   tCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGACAGAGACTGTGGTTAGTCAGGCGaa                               >  2:584958/1‑149 (MQ=255)
                                                                                                                    cTTATTTCCCTTGCATAAAAAAGCCAGCCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAAt                              <  3:257879/149‑1 (MQ=255)
                                                                                                                            ccTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGtt                      >  4:834465/1‑149 (MQ=255)
                                                                                                                            ccTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGtt                      >  4:817994/1‑149 (MQ=255)
                                                                                                                            ccTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGtt                      >  1:486805/1‑149 (MQ=255)
                                                                                                                             cTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGttt                     <  3:817994/149‑1 (MQ=255)
                                                                                                                                gCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGc                  <  1:129754/149‑1 (MQ=255)
                                                                                                                                  aTAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCgg                <  2:361101/149‑1 (MQ=255)
                                                                                                                                  aTAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCgg                >  1:450241/1‑149 (MQ=255)
                                                                                                                                  aTAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCAGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCgg                >  3:836500/1‑149 (MQ=255)
                                                                                                                                   tAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGc               >  1:443369/1‑149 (MQ=255)
                                                                                                                                   gagaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGAt                                                       >  2:881596/4‑109 (MQ=255)
                                                                                                                                   gagaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGAt                                                       <  1:881596/106‑1 (MQ=255)
                                                                                                                                     gaaaaGCCAAGCTGCGCGTTCGCTTTTCCCGTTCAGGCTGGCTTATTTGCGTTCGTGCGCATGCTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTGAAGATAGAGACTGAGGTTAGTCAGGCGAATGCCATGTTTTGCGGCaa             >  2:807167/2‑149 (MQ=255)
                                                                                                                                      aaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAAt            <  4:524743/149‑1 (MQ=255)
                                                                                                                                         tgCCAACCCGCAGGTTGGCTATTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGGTAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTc         <  3:602338/148‑1 (MQ=255)
                                                                                                                                         aGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTc         >  1:736287/1‑149 (MQ=255)
                                                                                                                                          gCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCa        >  4:872690/1‑149 (MQ=255)
                                                                                                                                          gCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCa        >  2:156346/1‑149 (MQ=255)
                                                                                                                                           ccAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTg                   <  4:710385/137‑1 (MQ=255)
                                                                                                                                           ccAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTg                   >  3:710385/1‑137 (MQ=255)
                                                                                                                                           ccAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCAc       <  1:652572/149‑1 (MQ=255)
                                                                                                                                             aaCCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATATCACGc     >  3:511054/1‑149 (MQ=255)
                                                                                                                                                ccGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCACGCTGa  >  2:285451/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCACGCTGA  >  NC_000913/710025‑710317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: