Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 786,613 C→A 9.7% A327A (GCC→GCA aroG → 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, phenylalanine repressible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913786,6130CA 9.7% 374.8 / 20.4 110A327A (GCC→GCAaroG3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, phenylalanine repressible
Reads supporting (aligned to +/- strand):  ref base C (44/55);  new base A (5/6);  total (49/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGC  >  NC_000913/786466‑786757
                                                                                                                                                   |                                                                                                                                                
gatggatgTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCTAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCt                                                                                                                                                 <  3:78934/149‑1 (MQ=255)
       gTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCAGGCATCgg                                                                                                                                          <  3:252961/149‑1 (MQ=255)
        tttGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCATAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGc                                                                                                                                         <  3:509341/149‑1 (MQ=255)
        tttGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCAGGCATCggt                                                                                                                                         <  2:696786/149‑2 (MQ=255)
           gtgCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTgg                                                                                                                                      <  3:70191/149‑1 (MQ=255)
            tgCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTggg                                                                                                                                     <  2:810800/149‑1 (MQ=255)
              cTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGaa                                                                                                                                   <  3:397738/149‑1 (MQ=255)
              cTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGaa                                                                                                                                   >  2:352401/1‑149 (MQ=255)
                 aCGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATGTGGTGGAAGGCAATCAGAGCCTCGCGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAgct                                                                                                                                >  2:610398/1‑147 (MQ=255)
                   gTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATAc                                                                                                                              <  2:336499/149‑1 (MQ=255)
                      tgtcagcagATTGCAGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGATAGCCATCTGGAGGAAGGCAATCAGAGCCTCGAGAGCGGGCAGCCGCTGGCCTATGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGa                                                                                                                           <  4:543307/146‑1 (MQ=255)
                         cagcagATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCt                                                                                                                                                 <  3:641978/124‑1 (MQ=255)
                         cagcagATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCt                                                                                                                                                 >  4:641978/1‑124 (MQ=255)
                         cagcagATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGc                                                                                                                        >  1:178841/1‑149 (MQ=255)
                         cagcagATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCAGGCATCggtgatg                                                                                                                                     >  1:37037/1‑131 (MQ=255)
                         cagcagATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCAGGCATCggtgatg                                                                                                                                     <  2:37037/136‑6 (MQ=255)
                           gcagATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGctc                                                                                                                      >  4:40363/1‑149 (MQ=255)
                            cagATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGctct                                                                                                                     <  3:264073/149‑1 (MQ=255)
                             agATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTg                                                                                                                    <  3:709865/149‑1 (MQ=255)
                              gATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGt                                                                                                                   >  1:362036/1‑149 (MQ=255)
                               aTTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGtt                                                                                                                  >  1:636033/1‑149 (MQ=255)
                                ttGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCAGGCATCggtga                                                                                                                                       <  3:641354/127‑4 (MQ=255)
                                ttGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCAGGCATCggtga                                                                                                                                       >  4:641354/1‑124 (MQ=255)
                                      gTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATAcc                                                                                                                             <  4:684981/131‑1 (MQ=255)
                                      gTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATAcc                                                                                                                             >  3:684981/1‑131 (MQ=255)
                                       tGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAAc                                                                                                          <  3:796381/149‑1 (MQ=255)
                                       tGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAAc                                                                                                          <  4:491805/149‑1 (MQ=255)
                                             aaaGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGa                                                                                                    <  2:126502/149‑1 (MQ=255)
                                               aGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAAt                                                                                                  <  3:505204/149‑1 (MQ=255)
                                                ggCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATg                                                                                                 <  1:31877/149‑1 (MQ=255)
                                                ggCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATg                                                                                                 <  2:337001/149‑1 (MQ=255)
                                                 gCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGc                                                                                                <  3:702119/149‑1 (MQ=255)
                                                   cATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTTGCGAATGCAg                                                                                              >  2:628229/1‑149 (MQ=255)
                                                   cATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAg                                                                                              >  2:766243/1‑149 (MQ=255)
                                                    attattGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGt                                                                                             >  4:666037/1‑149 (MQ=255)
                                                     ttattGGCGTGATGGTGGAAAGCCCTCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCCAATGCAGTa                                                                                            >  3:744637/1‑149 (MQ=255)
                                                          ggCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAgc                                                                                       <  2:49884/149‑1 (MQ=255)
                                                          ggCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAgc                                                                                       <  3:755068/149‑1 (MQ=255)
                                                                aTGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTcg                                                                                 <  1:766243/149‑1 (MQ=255)
                                                                   gtggAAAGCCATCTGGTGGAAGGGAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCgg                                                                              <  4:390309/149‑1 (MQ=255)
                                                                   gtggAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCgg                                                                              <  1:406836/149‑1 (MQ=255)
                                                                   gtggAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCAAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCgg                                                                              >  4:834717/1‑149 (MQ=255)
                                                                    tggAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCggg                                                                             >  1:844482/1‑149 (MQ=255)
                                                                           ccATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGtt                                                                      <  3:649285/149‑1 (MQ=255)
                                                                             aTCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTa                                                                    <  2:362036/149‑1 (MQ=255)
                                                                              tCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAg                                                                         >  3:71838/1‑143 (MQ=255)
                                                                              tCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAg                                                                         <  4:71838/143‑1 (MQ=255)
                                                                              tCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTaa                                                                   <  4:424175/149‑1 (MQ=255)
                                                                                tggtggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAAtt                                                                 >  1:846099/1‑149 (MQ=255)
                                                                                  gtggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGt                                                               >  4:734581/1‑149 (MQ=255)
                                                                                   tggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTc                                                              <  2:846099/149‑1 (MQ=255)
                                                                                   tggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTc                                                              >  2:454330/1‑149 (MQ=255)
                                                                                   tggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTc                                                              <  2:500124/149‑1 (MQ=255)
                                                                                   tggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTc                                                              <  1:352401/149‑1 (MQ=255)
                                                                                   tggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTc                                                              <  2:575984/149‑1 (MQ=255)
                                                                                    ggAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCg                                                             <  3:516446/149‑1 (MQ=255)
                                                                                        ggCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATg                                                         >  2:771012/1‑149 (MQ=255)
                                                                                        ggCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATg                                                         >  2:772649/1‑149 (MQ=255)
                                                                                           aaTCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATgcgc                                                      >  4:213125/1‑149 (MQ=255)
                                                                                                     tCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTg                                            >  2:137259/1‑149 (MQ=255)
                                                                                                     tCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTg                                            >  4:501657/1‑149 (MQ=255)
                                                                                                       tagagCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCATGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAg                                                                                              <  4:556350/96‑1 (MQ=255)
                                                                                                       gagaGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAg                                                                                              >  3:556350/1‑97 (MQ=255)
                                                                                                        agagCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCg                                         >  1:479929/1‑149 (MQ=255)
                                                                                                            cGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATc                                     <  3:213125/149‑1 (MQ=255)
                                                                                                             ggggAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATcc                                    <  1:628229/149‑1 (MQ=255)
                                                                                                               ggAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGa                                  <  2:178841/149‑1 (MQ=255)
                                                                                                               ggAGCCGCTGGCCTACGCTAAGAGCATCACCGATGCCTGCATCGGCTGGTAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTCTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGa                                  <  4:744637/149‑1 (MQ=255)
                                                                                                                       tGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATcac                          <  2:376907/149‑1 (MQ=255)
                                                                                                                           cTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACcaca                      >  1:21386/1‑149 (MQ=255)
                                                                                                                           cTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACcaca                      >  2:697932/1‑149 (MQ=255)
                                                                                                                             aCGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAgg                    <  1:437244/149‑1 (MQ=255)
                                                                                                                              tggTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCTAATGCAGTATAAGCGCGTAGCGGGTAAGGTTTAATTGTCGGATGCTCCATCAGAGTGGCGTATCCGATGAATCACCACAGGc                   <  2:639860/148‑1 (MQ=255)
                                                                                                                                gTAAGAGCATCACCTATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCaga                                               >  1:149498/1‑119 (MQ=255)
                                                                                                                                gTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCaga                                               <  4:239027/119‑1 (MQ=255)
                                                                                                                                gTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCaga                                               >  3:239027/1‑119 (MQ=255)
                                                                                                                                gTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCt                 <  3:40363/149‑1 (MQ=255)
                                                                                                                                gTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCt                 >  3:862453/1‑149 (MQ=255)
                                                                                                                                 tAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTg                >  1:783442/1‑149 (MQ=255)
                                                                                                                                  aaGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGt                                                                       <  3:355245/93‑1 (MQ=255)
                                                                                                                                  aaGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGt                                                                       >  4:355245/1‑93 (MQ=255)
                                                                                                                                  aaGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGa               <  1:654399/149‑1 (MQ=255)
                                                                                                                                   agagCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGAt              <  1:697932/149‑1 (MQ=255)
                                                                                                                                    gagCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATa             >  4:417640/1‑149 (MQ=255)
                                                                                                                                     agCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGc                                          <  4:226398/119‑1 (MQ=255)
                                                                                                                                     agCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGc                                          >  3:226398/1‑119 (MQ=255)
                                                                                                                                     agCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATaa            >  3:211550/1‑149 (MQ=255)
                                                                                                                                       cccagccGATGCAGGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGt          >  3:154001/6‑149 (MQ=255)
                                                                                                                                        aTCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGATTGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTc         <  3:834717/149‑1 (MQ=255)
                                                                                                                                        aTCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTaa                                                                   <  4:260555/91‑1 (MQ=255)
                                                                                                                                        aTCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTaa                                                                   >  3:260555/1‑91 (MQ=255)
                                                                                                                                        aTCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGa                                                           >  4:686565/1‑99 (MQ=255)
                                                                                                                                        aTCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGa                                                           <  3:686565/99‑1 (MQ=255)
                                                                                                                                         tCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTcg        >  3:794936/1‑149 (MQ=255)
                                                                                                                                         tCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTcg        >  3:744342/1‑149 (MQ=255)
                                                                                                                                          agccGATGCAGGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGc                                          >  3:755227/3‑114 (MQ=255)
                                                                                                                                          agccGATGCAGGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGc                                          <  4:755227/112‑1 (MQ=255)
                                                                                                                                           gccGATGCAGGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTcgcg      >  3:795676/2‑149 (MQ=255)
                                                                                                                                            ccGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCgcgc     >  4:311881/1‑149 (MQ=255)
                                                                                                                                               aTGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGc  >  3:199550/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GATGGATGTTTGTGCTGACGTTTGCCAGCAGATTGCCGGTGGCGAAAAGGCCATTATTGGCGTGATGGTGGAAAGCCATCTGGTGGAAGGCAATCAGAGCCTCGAGAGCGGGGAGCCGCTGGCCTACGGTAAGAGCATCACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGC  >  NC_000913/786466‑786757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: