Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 788,385 G→T 6.5% T151T (ACC→ACA) ‡ galM ← aldose 1‑epimerase; type‑1 mutarotase; galactose mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913788,3850GT 6.5% 307.5 / 10.1 93T151T (ACC→ACA) ‡galMaldose 1‑epimerase; type‑1 mutarotase; galactose mutarotase
Reads supporting (aligned to +/- strand):  ref base G (46/40);  new base T (3/3);  total (49/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

GCAGATATTCGTCCGCCAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCT  >  NC_000913/788237‑788533
                                                                                                                                                    |                                                                                                                                                    
gCAGATATTCGTCCGCCAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTAtcgtcg                                                                                                                                                      >  4:749780/1‑149 (MQ=255)
gCAGATATTCGTCCGCCAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTAtcgtcg                                                                                                                                                      <  3:308117/149‑1 (MQ=255)
      atTCGTCCGCCAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATTGTCGGTCAGa                                                                                                                                                >  3:109361/1‑149 (MQ=255)
      atTCGTCCGCCAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGa                                                                                                                                                <  2:831921/149‑1 (MQ=255)
            ccGCCAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCTGACCGACGataa                                                                                                                                          <  3:719799/149‑2 (MQ=255)
            ccGCCAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCACGCCGTCAAGATCGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCTATAAGTAATGGAGATACGGTTATCGTCTGACCGACGataa                                                                                                                                          <  2:276886/149‑2 (MQ=255)
             cGCCAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCTGACCGACGataac                                                                                                                                         <  3:15329/149‑3 (MQ=255)
              gCCAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTg                                                                                                                                        >  2:247557/1‑149 (MQ=255)
               ccAGAATCTGCAACTTGTGATTGCGCTCGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGCTCAGACGATATTGc                                                                                                                                       >  3:547715/1‑149 (MQ=255)
                cAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCa                                                                                                                                      <  1:823456/149‑1 (MQ=255)
                  gAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCAcc                                                                                                                                    >  4:489603/1‑149 (MQ=255)
                     tCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTc                                                                                                                                 <  3:323660/149‑1 (MQ=255)
                       tGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTcgc                                                                                                                               >  3:591681/1‑149 (MQ=255)
                       tGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTcgc                                                                                                                               <  4:718889/149‑1 (MQ=255)
                         cAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCgcgc                                                                                                                             <  2:652336/149‑1 (MQ=255)
                          aaCTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGTTTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGcc                                                                                                                            >  4:535463/1‑149 (MQ=255)
                             ttGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCgag                                                                                                                         >  4:82821/1‑149 (MQ=255)
                             ttGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCgag                                                                                                                         >  1:550815/1‑149 (MQ=255)
                              tgtgATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCgaga                                                                                                                        <  2:641888/149‑1 (MQ=255)
                                  aTTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTAc                                                                                                                    <  4:111692/149‑1 (MQ=255)
                                  aTTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTAc                                                                                                                    <  3:88172/149‑1 (MQ=255)
                                       gcACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCggg                                                                                                               >  3:298675/1‑149 (MQ=255)
                                         aCGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGaa                                                                                                             >  4:415952/1‑149 (MQ=255)
                                         aCGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGaa                                                                                                             >  3:708431/1‑149 (MQ=255)
                                         aCGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGaa                                                                                                             >  2:471707/1‑149 (MQ=255)
                                            tCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAAcc                                                                                                          <  4:591681/149‑1 (MQ=255)
                                              aGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCt                                                                                                        >  3:706541/1‑149 (MQ=255)
                                              aGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCt                                                                                                        >  3:575081/1‑149 (MQ=255)
                                               gACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCga                                                                                                                          >  4:24869/1‑130 (MQ=255)
                                               gACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCga                                                                                                                          <  3:24869/130‑1 (MQ=255)
                                                aCTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGa                                                                                                      >  3:419361/1‑149 (MQ=255)
                                                aCTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGa                                                                                                      <  4:708431/149‑1 (MQ=255)
                                                aCTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGa                                                                                                      <  4:706541/149‑1 (MQ=255)
                                                     tCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCAcc                                                                                                 >  2:731251/1‑149 (MQ=255)
                                                     tCGCCATCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATc                                                                                                    >  4:124450/1‑146 (MQ=255)
                                                     tCGCCATCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATc                                                                                                    <  3:124450/146‑1 (MQ=255)
                                                       gCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACcat                                                                                               >  4:208476/1‑149 (MQ=255)
                                                         cGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACcatca                                                                                             <  1:731251/149‑1 (MQ=255)
                                                          gTCAAGATTGAAATAGACGTGATTAGTAATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACcatcat                                                                                            >  2:588614/1‑149 (MQ=255)
                                                           tCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCatcatc                                                                                           >  4:101281/1‑149 (MQ=255)
                                                           tCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCatcatc                                                                                           >  1:251485/1‑149 (MQ=255)
                                                            cAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCt                                                                                          <  3:224550/149‑1 (MQ=255)
                                                            cAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCt                                                                                          <  1:186012/149‑1 (MQ=255)
                                                                    aaaTAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAg                                                                                  <  1:471707/149‑1 (MQ=255)
                                                                    aaaTAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAg                                                                                  <  3:101281/149‑1 (MQ=255)
                                                                    aaaTAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAg                                                                                  >  1:208097/1‑149 (MQ=255)
                                                                     aaTAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAgg                                                                                 <  4:716293/149‑1 (MQ=255)
                                                                         gACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCaa                                                                             >  4:279966/1‑149 (MQ=255)
                                                                         gACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCaa                                                                             >  3:856565/1‑149 (MQ=255)
                                                                          aCGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGAt                                                                                                     <  2:771832/124‑1 (MQ=255)
                                                                          aCGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATAGTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCATGGCaaa                                                                            <  2:550815/149‑1 (MQ=255)
                                                                          aCGGGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGAt                                                                                                     >  1:771832/1‑124 (MQ=255)
                                                                            gTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACa                                                                          >  4:636615/1‑149 (MQ=255)
                                                                            gTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACa                                                                          >  3:174395/1‑149 (MQ=255)
                                                                              gATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGc                                                                        <  1:185816/149‑1 (MQ=255)
                                                                              gATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGc                                                                        >  4:231384/1‑149 (MQ=255)
                                                                              gATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGc                                                                        <  1:429834/149‑1 (MQ=255)
                                                                              gATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGc                                                                        >  3:730768/1‑149 (MQ=255)
                                                                                ttAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCAc                                                                      <  1:841007/149‑1 (MQ=255)
                                                                                    tCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTTCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGa                                                                  >  2:419711/1‑149 (MQ=255)
                                                                                    acATATTCACCGGTCAAGGTTTATCAACTGTCGCGCGATAAGTAATGGAGATACGGTTATCGGCCGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGTCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGa                                                                  <  4:431134/148‑1 (MQ=255)
                                                                                      atatTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGaa                                                                                       <  1:799699/126‑1 (MQ=255)
                                                                                      atatTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGaa                                                                                       >  2:799699/1‑126 (MQ=255)
                                                                                        atTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGAt                                                              <  4:174395/149‑1 (MQ=255)
                                                                                         ttgaCCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATc                                                             <  4:816907/146‑1 (MQ=255)
                                                                                         ttCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAgg                                                                                 >  1:50659/1‑129 (MQ=255)
                                                                                         ttCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAgg                                                                                 <  2:50659/129‑1 (MQ=255)
                                                                                         ttCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATc                                                             <  4:265431/149‑1 (MQ=255)
                                                                                            aCCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGtt                                                          >  2:558915/1‑149 (MQ=255)
                                                                                            aCCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGtt                                                          >  3:2259/1‑149 (MQ=255)
                                                                                              cGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCt                                                        <  3:600128/149‑1 (MQ=255)
                                                                                              cGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCt                                                        <  4:856565/149‑1 (MQ=255)
                                                                                                ggCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTgg                                                      <  4:103472/149‑1 (MQ=255)
                                                                                                ggCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTgg                                                      <  2:532572/149‑1 (MQ=255)
                                                                                                 gCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGt                                                     >  2:361304/1‑149 (MQ=255)
                                                                                                        ttATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAAt                                              >  2:500037/1‑149 (MQ=255)
                                                                                                             aaCTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGcc                                         >  1:527258/1‑149 (MQ=255)
                                                                                                              aCTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCa                                        >  2:502058/1‑149 (MQ=255)
                                                                                                               cTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAg                                       >  4:510683/1‑149 (MQ=255)
                                                                                                               cTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAg                                       <  4:298675/149‑1 (MQ=255)
                                                                                                                       cgATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGTAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGttt                               <  4:880139/149‑1 (MQ=255)
                                                                                                                             gTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGaa                         <  2:158214/149‑1 (MQ=255)
                                                                                                                             gTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGaa                         >  3:249540/1‑149 (MQ=255)
                                                                                                                                aTGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAAccc                      >  4:452620/1‑149 (MQ=255)
                                                                                                                                   gagaTACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTc                   <  2:251485/149‑1 (MQ=255)
                                                                                                                                      ggtgcaatATCGTCTGACCGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCgg                >  1:314406/8‑149 (MQ=255)
                                                                                                                                       tACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGc               <  3:452620/149‑1 (MQ=255)
                                                                                                                                       gtgcaatATCGTCTGACCGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGc               >  3:3162/7‑149 (MQ=255)
                                                                                                                                        aCGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGt                             >  2:588269/1‑134 (MQ=255)
                                                                                                                                        aCGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGt                             <  1:588269/134‑1 (MQ=255)
                                                                                                                                        aCGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGcc              >  3:847094/1‑149 (MQ=255)
                                                                                                                                          ggTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGccc                                                                                                         <  1:569429/56‑1 (MQ=255)
                                                                                                                                          ggTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGccc                                                                                                         >  2:569429/1‑56 (MQ=255)
                                                                                                                                           gTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCccgc           >  1:618926/1‑149 (MQ=255)
                                                                                                                                           aatATCGTCTGACCGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACTATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCccgc           >  4:539906/3‑149 (MQ=255)
                                                                                                                                                 gtcgGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCa     >  3:598561/1‑149 (MQ=255)
                                                                                                                                                  tcgGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAg    >  1:491879/1‑149 (MQ=255)
                                                                                                                                                    ggTCAGACGATATTTCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCt  <  1:419711/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAGATATTCGTCCGCCAGAATCTGCAACTTGTGATTGCGCACGTCAGACTGCTCGCCGTCAAGATTGAAATAGACGTGATTAGTCATATTCACCGGGCAAGGTTTATCAACTGTGGCGCGATAAGTAATGGAGATACGGTTATCGTCGGTCAGACGATATTGCACCGTCGCGCCGAGATTACCCGGGAAGCCCTGATCACCATCATCTGAACTCAGGGCAAACAGCACCTGACGATCGTTCTGGTTCACAATCTGCCAGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCT  >  NC_000913/788237‑788533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: