Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,004,746 C→A 5.5% intergenic (+89/‑22) ycbF → / → pyrD putative periplasmic pilin chaperone/dihydro‑orotate oxidase, FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,004,7460CA 5.5% 287.5 / 4.9 85intergenic (+89/‑22)ycbF/pyrDputative periplasmic pilin chaperone/dihydro‑orotate oxidase, FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (35/45);  new base A (3/2);  total (38/47)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

TGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTG  >  NC_000913/1004598‑1004893
                                                                                                                                                    |                                                                                                                                                   
tGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTGAATCCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGcac                                                                                                                                                     >  4:773909/1‑149 (MQ=255)
tGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAAACTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGcac                                                                                                                                                     >  3:469005/1‑149 (MQ=255)
    gggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCg                                                                                                                                                 <  3:306018/149‑1 (MQ=255)
    gggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCg                                                                                                                                                 <  4:33374/149‑1 (MQ=255)
     ggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCgg                                                                                                                                                <  1:763418/149‑1 (MQ=255)
     ggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCgg                                                                                                                                                <  4:600376/149‑1 (MQ=255)
        gATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGaa                                                                                                                                             >  1:649865/1‑149 (MQ=255)
        gATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTGTGCAAACCggggg                                                                                                                                             <  2:161291/149‑3 (MQ=255)
         aTAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTGTGCAAACCgggggc                                                                                                                                            <  1:370651/149‑4 (MQ=255)
            aaTGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTTCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCa                                                                                                                                         >  2:293879/1‑149 (MQ=255)
            aaTGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCa                                                                                                                                         >  2:245285/1‑149 (MQ=255)
             aTGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGTGAATCCAg                                                                                                                                        <  4:242420/149‑1 (MQ=255)
             aTGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAg                                                                                                                                        <  3:689041/149‑1 (MQ=255)
             aTGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAg                                                                                                                                        <  1:245285/149‑1 (MQ=255)
               gATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGga                                                                                                                                      <  2:266338/149‑1 (MQ=255)
               gATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGga                                                                                                                                      <  4:418473/149‑1 (MQ=255)
                   aCGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGt                                                                                                                                  <  4:877771/149‑1 (MQ=255)
                     ggcggGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTc                                                                                                                                <  2:397843/149‑1 (MQ=255)
                      gcggGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAgg                                                                                                                                       >  1:751501/1‑141 (MQ=255)
                      gcggGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAgg                                                                                                                                       <  2:751501/141‑1 (MQ=255)
                      gcggGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCa                                                                                                                               <  1:689179/149‑1 (MQ=255)
                          gTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGta                                                                                                                           <  3:561659/149‑1 (MQ=255)
                                     agaagGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCttcg                                                                                                                >  3:460021/1‑149 (MQ=255)
                                      gaagGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCttcgt                                                                                                               <  1:20579/149‑1 (MQ=255)
                                       aagGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCttcgtt                                                                                                              >  2:48924/1‑149 (MQ=255)
                                       aagGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCttcgtt                                                                                                              >  4:576903/1‑149 (MQ=255)
                                        agGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCttcgttc                                                                                                             <  3:643932/149‑1 (MQ=255)
                                        agGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCttcgttc                                                                                                             <  2:851949/149‑1 (MQ=255)
                                        agGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCttcgttc                                                                                                             <  1:132341/149‑1 (MQ=255)
                                        agGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCttcgttc                                                                                                             <  1:20187/149‑1 (MQ=255)
                                          gAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTTCTACCCCTtcgttcgt                                                                                                           >  3:753040/1‑149 (MQ=255)
                                            gCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTaa                                                                                                         >  1:813682/1‑149 (MQ=255)
                                             cAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTaaa                                                                                                        <  3:219525/149‑1 (MQ=255)
                                             cAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTaaa                                                                                                        <  1:172172/149‑1 (MQ=255)
                                                  tCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCt                                                                                                   >  3:216391/1‑149 (MQ=255)
                                                      cccTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGCCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTc                                                                                               >  4:290752/1‑149 (MQ=255)
                                                        cTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCa                                                                                             >  2:188309/1‑149 (MQ=255)
                                                           aaaCAGGTTCGGAAAACGTTTGCGTTGTTTTTGCCGCAGGGCAATTCCCTTTTGGTCCGAACTCGCACATAATAAGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGCTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCt                                                                                          >  4:610493/1‑149 (MQ=255)
                                                               aGGTTCGGCAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGCTCCGAACTCGCTCATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGTGCTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGAt                                                                                      <  4:542457/149‑1 (MQ=255)
                                                                    cGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCaga                                                                                 <  3:679432/149‑1 (MQ=255)
                                                                     ggAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCagag                                                                                >  3:433095/1‑149 (MQ=255)
                                                                      gAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAgc                                                                               >  3:663535/1‑149 (MQ=255)
                                                                       aaaaCGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGtt                                                                                                                                 >  2:405199/1‑98 (MQ=255)
                                                                       aaaaCGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGtt                                                                                                                                 <  1:405199/98‑1 (MQ=255)
                                                                                gCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGa                                                                     <  3:386837/149‑1 (MQ=255)
                                                                                gCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGa                                                                     <  4:433095/149‑1 (MQ=255)
                                                                                 cGTTTTTTTTGCCGCAGGCCAATTCCCTTTTGGTCCGAACTCGCACAAAATACGCCCCCGGATTGCAAACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGGTCCAGAGCGCGCGCATAAg                                                                    >  2:71460/1‑149 (MQ=255)
                                                                                  gTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGt                                                                   >  1:364938/1‑149 (MQ=255)
                                                                                   ttttttttGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGtt                                                                  <  3:791261/149‑1 (MQ=255)
                                                                                        tttGCCGCAGGTCCATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGGTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACtt                                                             <  2:883378/149‑1 (MQ=255)
                                                                                              gCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTc                                                          >  1:758243/1‑146 (MQ=255)
                                                                                              gCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTc                                                          <  2:758243/146‑1 (MQ=255)
                                                                                               cAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCa                                                      >  3:699355/1‑149 (MQ=255)
                                                                                                  gTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAAtt                                                   <  1:293879/149‑1 (MQ=255)
                                                                                                     aaTTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACg                                                >  1:762497/1‑149 (MQ=255)
                                                                                                      aTTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGc                                               >  1:540882/1‑149 (MQ=255)
                                                                                                       ttCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTAc                                                               >  3:781790/1‑132 (MQ=255)
                                                                                                       ttCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTAc                                                               <  4:781790/132‑1 (MQ=255)
                                                                                                       ttCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGcc                                              >  1:161367/1‑149 (MQ=255)
                                                                                                       ttCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTCCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGcc                                              >  2:868721/1‑149 (MQ=255)
                                                                                                          ccTTTTGGTCCGAACTCGCACATAATACGCCCCTGGTTTGCACACCGGGAAGCTAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTa                                           <  4:595836/149‑1 (MQ=255)
                                                                                                          ccTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTa                                           <  1:71460/149‑1 (MQ=255)
                                                                                                           cTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTAt                                          <  3:282184/149‑1 (MQ=255)
                                                                                                           cTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTAt                                          >  3:20935/1‑149 (MQ=255)
                                                                                                               tGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGATTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCAGGAGTTTACTTTTCAGCAATTACGCCGTATTACa                                      <  2:438775/149‑1 (MQ=255)
                                                                                                               tGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACa                                      <  4:460021/149‑1 (MQ=255)
                                                                                                                   ccGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGaa                                  >  3:573991/1‑149 (MQ=255)
                                                                                                                    cGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAAc                                 >  1:290477/1‑149 (MQ=255)
                                                                                                                    cGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAAc                                 <  3:414950/149‑1 (MQ=255)
                                                                                                                    cGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGATCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACtt                                                             >  1:675049/1‑121 (MQ=255)
                                                                                                                    cGAACTCGCACATAATACGCCCCCGGTTTGAACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACtt                                                             <  2:675049/121‑1 (MQ=255)
                                                                                                                         tCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAg                                                                                                       <  4:85108/74‑1 (MQ=255)
                                                                                                                         tCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAg                                                                                                       >  3:85108/1‑74 (MQ=255)
                                                                                                                          cGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGtt                           <  3:85701/149‑1 (MQ=255)
                                                                                                                           gCACATAATACTCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCCTGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGttt                          >  4:581204/1‑149 (MQ=255)
                                                                                                                           gCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGttt                          >  3:276586/1‑149 (MQ=255)
                                                                                                                             acaTAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCa                                                         >  1:767969/1‑116 (MQ=255)
                                                                                                                             acaTAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCa                                                         <  2:767969/116‑1 (MQ=255)
                                                                                                                              caTAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGaa                       <  4:20935/149‑1 (MQ=255)
                                                                                                                              caTAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGaa                       <  2:741900/149‑1 (MQ=255)
                                                                                                                                taataCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGc                     <  4:216391/149‑1 (MQ=255)
                                                                                                                                 aataCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCa                    >  4:340701/1‑149 (MQ=255)
                                                                                                                                 aataCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCa                    >  3:765075/1‑149 (MQ=255)
                                                                                                                                  ataCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCAc                   <  1:48924/149‑1 (MQ=255)
                                                                                                                                    ggattcccGGTGTGCAAACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTg                 >  2:556064/6‑149 (MQ=255)
                                                                                                                                        tcccGGTGTGCAAACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGc             >  1:888591/2‑149 (MQ=255)
                                                                                                                                             gTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAg        >  2:315383/1‑149 (MQ=255)
                                                                                                                                                   acacCGGGAATCTAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTg  >  3:191907/1‑149 (MQ=255)
                                                                                                                                                   acacCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTg  >  1:161517/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTG  >  NC_000913/1004598‑1004893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑