Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,036,227 T→A 7.0% L153H (CTT→CAT) ‡ hyaD → hydrogenase 1 maturation protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,036,2270TA 7.0% 369.5 / 10.1 109L153H (CTT→CAT) ‡hyaDhydrogenase 1 maturation protease
Reads supporting (aligned to +/- strand):  ref base T (48/53);  new base A (4/4);  total (52/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 112 total aligned reads displayed.

TTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTG  >  NC_000913/1036079‑1036375
                                                                                                                                                    |                                                                                                                                                    
ttGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCt                                                                                                                                                      <  4:48759/149‑1 (MQ=255)
      cTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTtgctgc                                                                                                                                                <  3:421430/149‑1 (MQ=255)
         gCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTg                                                                                                                                             >  1:756916/1‑149 (MQ=255)
         gCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCAAGCTGCgcc                                                                                                                                             <  4:639816/149‑3 (MQ=255)
         gCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCAAGCTGCgcc                                                                                                                                             <  2:80395/149‑3 (MQ=255)
          cGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgg                                                                                                                                            <  4:735535/149‑1 (MQ=255)
           ggATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCGCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg                                                                                                                                           >  4:742305/1‑149 (MQ=255)
                tCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGGCTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAAtt                                                                                                                                      <  1:638631/149‑1 (MQ=255)
                    cgGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGc                                                                                                                                  <  1:157544/149‑1 (MQ=255)
                      gACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCg                                                                                                                                >  1:577350/1‑149 (MQ=255)
                         aTCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCaa                                                                                                                             >  2:765483/1‑149 (MQ=255)
                         aTCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCaa                                                                                                                             >  1:700858/1‑149 (MQ=255)
                                 gCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGCACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTaa                                                                                                                     <  1:576368/149‑1 (MQ=255)
                                 gCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTaa                                                                                                                     <  2:458530/149‑1 (MQ=255)
                                 gCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTaa                                                                                                                     >  1:163012/1‑149 (MQ=255)
                                  cacaTATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAAt                                                                                                                    <  1:258375/149‑1 (MQ=255)
                                  cacaTATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAAt                                                                                                                    <  1:609050/149‑1 (MQ=255)
                                  cacaTATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAAt                                                                                                                    >  3:823699/1‑149 (MQ=255)
                                        ttGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATcg                                                                                                              >  3:422699/1‑149 (MQ=255)
                                          gCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCAAGCTGCgccag                                                                                                                                           >  3:663414/1‑114 (MQ=255)
                                           cccTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCgcgc                                                                                                           >  4:402087/1‑149 (MQ=255)
                                            ccTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCt                                                                                                          >  3:363060/1‑149 (MQ=255)
                                             cTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTg                                                                                                         >  1:622072/1‑149 (MQ=255)
                                             cTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTg                                                                                                         >  2:794036/1‑149 (MQ=255)
                                              tcgtcgGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGt                                                                                                        >  2:41050/1‑149 (MQ=255)
                                              tcgtcgGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCAAGCTGCgccagc                                                                                                                                          >  2:868061/1‑110 (MQ=255)
                                              tcgtcgGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCAAGCTGCgccagc                                                                                                                                          <  1:868061/115‑6 (MQ=255)
                                                gtcgGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGtct                                                                                                      >  1:541670/1‑149 (MQ=255)
                                                   ggTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCaa                                                                                                   >  2:693555/1‑149 (MQ=255)
                                                   ggTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCaa                                                                                                   >  1:227125/1‑149 (MQ=255)
                                                    gTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAAt                                                                                                  <  4:486996/149‑1 (MQ=255)
                                                    gTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAAt                                                                                                  >  1:897595/1‑149 (MQ=255)
                                                    gTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCACAAt                                                                                                  >  3:275219/1‑149 (MQ=255)
                                                      cTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTa                                                                                                <  2:577350/149‑1 (MQ=255)
                                                        gCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATg                                                                                              >  3:597236/1‑149 (MQ=255)
                                                         cAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGa                                                                                             <  4:128932/149‑1 (MQ=255)
                                                         cAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGAGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGa                                                                                             <  2:647424/149‑1 (MQ=255)
                                                           aCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGAct                                                                                           >  3:517622/1‑149 (MQ=255)
                                                            cccGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActg                                                                                          >  2:568303/1‑149 (MQ=255)
                                                             ccGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAAtt                                                                                                                                      >  1:639788/1‑104 (MQ=255)
                                                             ccGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAAtt                                                                                                                                      <  2:639788/104‑1 (MQ=255)
                                                             ccGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgt                                                                                         <  2:158510/149‑1 (MQ=255)
                                                             ccGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgt                                                                                         <  3:443339/149‑1 (MQ=255)
                                                               gCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtct                                                                                       >  2:270136/1‑149 (MQ=255)
                                                               gCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtct                                                                                       <  2:727850/149‑1 (MQ=255)
                                                               gCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtct                                                                                       <  1:560257/149‑1 (MQ=255)
                                                                 aaTGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtctgt                                                                                     <  1:717017/149‑1 (MQ=255)
                                                                    gCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGa                                                                                  >  4:712074/1‑149 (MQ=255)
                                                                     cTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGAt                                                                                 <  2:269362/149‑1 (MQ=255)
                                                                     cTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGAt                                                                                 <  2:408381/149‑1 (MQ=255)
                                                                      tCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATg                                                                                >  1:755045/1‑149 (MQ=255)
                                                                            acTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAAt                                                                          <  3:402087/149‑1 (MQ=255)
                                                                            acTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAAt                                                                          <  2:857502/149‑1 (MQ=255)
                                                                             cTACGGCGGTAGCCTGAGCGAACTCGCACGGGAGCAACTGCCCGCAGCGGAACAGGCGGCGCTGACGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAGTTATGACTGGCTGTCGATGGAAAAtt                                                                         >  4:680170/1‑149 (MQ=255)
                                                                               aCGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTAc                                                                       <  4:753924/149‑1 (MQ=255)
                                                                                     gTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGc                                                                 <  4:77236/149‑1 (MQ=255)
                                                                                     gTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGc                                                                 <  1:765483/149‑1 (MQ=255)
                                                                                              gCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTg                                                        >  2:714388/1‑149 (MQ=255)
                                                                                                gAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGTCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTgcg                                                      <  2:756916/149‑1 (MQ=255)
                                                                                                 aaCTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTgcgc                                                     <  1:329192/149‑1 (MQ=255)
                                                                                                 aaCTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTgcgc                                                     >  1:580994/1‑149 (MQ=255)
                                                                                                    tGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAg                                                  <  1:316711/149‑1 (MQ=255)
                                                                                                     ggCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGt                                                 >  3:562685/1‑149 (MQ=255)
                                                                                                         cGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtct                                                                                       <  3:192175/107‑1 (MQ=255)
                                                                                                         cGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtct                                                                                       >  4:192175/1‑107 (MQ=255)
                                                                                                         cGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCg                                             <  1:281983/149‑1 (MQ=255)
                                                                                                           ggAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGa                                           <  1:891354/149‑1 (MQ=255)
                                                                                                             aGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATg                                         >  3:21766/1‑149 (MQ=255)
                                                                                                              gCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGa                                           >  4:422891/1‑146 (MQ=255)
                                                                                                              gCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGa                                           <  3:422891/146‑1 (MQ=255)
                                                                                                              gCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGa                                        >  4:268045/1‑149 (MQ=255)
                                                                                                              gCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGACAGTACCGGTTGa                                        >  1:131552/1‑149 (MQ=255)
                                                                                                              gCAACTGCCCGCTGCGGAACAGGCGGCGCTGGAGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAACATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGa                                        <  3:742305/149‑1 (MQ=255)
                                                                                                                   tGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTATGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGGTGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACag                                   >  4:593640/1‑149 (MQ=255)
                                                                                                                        gCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAggagga                              >  3:506970/1‑149 (MQ=255)
                                                                                                                        gCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAggagga                              <  4:119085/149‑1 (MQ=255)
                                                                                                                        gCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCACAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAggagga                              <  2:737603/149‑1 (MQ=255)
                                                                                                                           gCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCa                           <  2:700858/149‑1 (MQ=255)
                                                                                                                            cGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTAt                                                                                               <  1:633713/80‑1 (MQ=255)
                                                                                                                            cGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTAt                                                                                               >  2:633713/1‑80 (MQ=255)
                                                                                                                             ggAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAgg                         <  1:714388/149‑1 (MQ=255)
                                                                                                                              taaCAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCACGCTGTCTCAATTATGACTGTCTGTCAATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAggg                        <  4:655355/148‑1 (MQ=255)
                                                                                                                              gAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAggg                        <  2:790189/149‑1 (MQ=255)
                                                                                                                              gAACAGGCGGCGCTGGCGCAGCTTGCGGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAAGTATGTCTGTCTGTCGGTGGACAATTACGACGGCGCTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAggg                        <  2:858806/149‑1 (MQ=255)
                                                                                                                                aCAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGAt                      <  4:138226/149‑1 (MQ=255)
                                                                                                                                  aGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGa                    <  4:777367/149‑1 (MQ=255)
                                                                                                                                  aGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGa                    >  1:57501/1‑149 (MQ=255)
                                                                                                                                    gcggcgCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGTAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGa                    >  2:724905/1‑147 (MQ=255)
                                                                                                                                    gcggcgCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGa                    <  1:724905/147‑1 (MQ=255)
                                                                                                                                      ggcgCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTATCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCaa                <  4:256750/149‑1 (MQ=255)
                                                                                                                                       gcgcTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAAc               <  4:628332/149‑1 (MQ=255)
                                                                                                                                           ccacgcAGCAAGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGaca           >  2:424979/4‑149 (MQ=255)
                                                                                                                                           ccacgcAGCAAGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGaca           >  2:576449/4‑149 (MQ=255)
                                                                                                                                              cgcAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACTAAGGCGTTCGCTTGCGCCAGTACCGGAGGACACAGGAGGAGCAGGGATGAGCAACGACACGc        >  4:762015/1‑149 (MQ=255)
                                                                                                                                               gcAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAggagga                              <  2:56237/126‑1 (MQ=255)
                                                                                                                                               gcAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAggagga                              >  1:56237/1‑126 (MQ=255)
                                                                                                                                               gcAGCTTGCGGCGTGGTGAATTGTACCGCAACCGGCTAATGAATGGCACTGTCTCGATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGGGCAACGACACGcc       >  4:468186/1‑149 (MQ=255)
                                                                                                                                                   cTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCAttt   >  1:281878/1‑149 (MQ=255)
                                                                                                                                                    ttGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTg  >  4:656348/1‑149 (MQ=255)
                                                                                                                                                    ttGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTg  >  4:195099/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTG  >  NC_000913/1036079‑1036375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: