Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,036,228 T→A 7.4% L153L (CTT→CTA) ‡ hyaD → hydrogenase 1 maturation protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,036,2280TA 7.4% 366.6 / 14.3 108L153L (CTT→CTA) ‡hyaDhydrogenase 1 maturation protease
Reads supporting (aligned to +/- strand):  ref base T (48/52);  new base A (4/4);  total (52/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 111 total aligned reads displayed.

CTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTG  >  NC_000913/1036085‑1036375
                                                                                                                                               |                                                                                                                                                   
cTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTtgctgc                                                                                                                                                <  3:421430/149‑1 (MQ=255)
   gCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTg                                                                                                                                             >  1:756916/1‑149 (MQ=255)
   gCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTg                                                                                                                                             <  1:610828/149‑1 (MQ=255)
   gCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCAAGCTGCgcc                                                                                                                                             <  4:639816/149‑3 (MQ=255)
   gCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCAAGCTGCgcc                                                                                                                                             <  2:80395/149‑3 (MQ=255)
    cGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTgg                                                                                                                                            <  4:735535/149‑1 (MQ=255)
     ggATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCGCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg                                                                                                                                           >  4:742305/1‑149 (MQ=255)
          tCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGGCTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAAtt                                                                                                                                      <  1:638631/149‑1 (MQ=255)
              cgGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGc                                                                                                                                  <  1:157544/149‑1 (MQ=255)
                gACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCg                                                                                                                                >  1:577350/1‑149 (MQ=255)
                   aTCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCaa                                                                                                                             >  2:765483/1‑149 (MQ=255)
                   aTCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCaa                                                                                                                             >  3:138226/1‑149 (MQ=255)
                   aTCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCaa                                                                                                                             >  1:700858/1‑149 (MQ=255)
                         cAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCt                                                                                                                       >  1:727850/1‑149 (MQ=255)
                           gCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGCACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTaa                                                                                                                     <  1:576368/149‑1 (MQ=255)
                           gCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTaa                                                                                                                     <  2:458530/149‑1 (MQ=255)
                           gCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTaa                                                                                                                     >  1:163012/1‑149 (MQ=255)
                            cacaTATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAAt                                                                                                                    >  3:823699/1‑149 (MQ=255)
                            cacaTATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAAt                                                                                                                    <  1:609050/149‑1 (MQ=255)
                            cacaTATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAAt                                                                                                                    <  1:258375/149‑1 (MQ=255)
                                  ttGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATcg                                                                                                              >  3:422699/1‑149 (MQ=255)
                                    gCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCAAGCTGCgccag                                                                                                                                           <  4:663414/118‑5 (MQ=255)
                                     cccTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCgcgc                                                                                                           >  4:402087/1‑149 (MQ=255)
                                      ccTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCt                                                                                                          >  3:363060/1‑149 (MQ=255)
                                      ccTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCt                                                                                                          >  3:442690/1‑149 (MQ=255)
                                       cTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTg                                                                                                         >  1:622072/1‑149 (MQ=255)
                                        tcgtcgGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGt                                                                                                        >  2:41050/1‑149 (MQ=255)
                                        tcgtcgGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCAAGCTGCgccagc                                                                                                                                          >  2:868061/1‑110 (MQ=255)
                                        tcgtcgGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCAAGCTGCgccagc                                                                                                                                          <  1:868061/115‑6 (MQ=255)
                                             ggTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCaa                                                                                                   >  1:227125/1‑149 (MQ=255)
                                              gTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAAt                                                                                                  >  1:897595/1‑149 (MQ=255)
                                              gTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAAt                                                                                                  <  4:486996/149‑1 (MQ=255)
                                              gTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCACAAt                                                                                                  >  3:275219/1‑149 (MQ=255)
                                                cTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTa                                                                                                <  2:577350/149‑1 (MQ=255)
                                                  gCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATg                                                                                              >  3:597236/1‑149 (MQ=255)
                                                   cAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGa                                                                                             <  4:128932/149‑1 (MQ=255)
                                                   cAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGAGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGa                                                                                             <  2:647424/149‑1 (MQ=255)
                                                     aCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGAct                                                                                           >  3:517622/1‑149 (MQ=255)
                                                      cccGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActg                                                                                          >  2:568303/1‑149 (MQ=255)
                                                       ccGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAAtt                                                                                                                                      <  2:639788/104‑1 (MQ=255)
                                                       ccGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAAtt                                                                                                                                      >  1:639788/1‑104 (MQ=255)
                                                       ccGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgt                                                                                         <  3:443339/149‑1 (MQ=255)
                                                       ccGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgt                                                                                         <  2:158510/149‑1 (MQ=255)
                                                        cGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtc                                                                                        <  2:376314/149‑1 (MQ=255)
                                                         gCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtct                                                                                       <  1:560257/149‑1 (MQ=255)
                                                         gCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtct                                                                                       >  2:270136/1‑149 (MQ=255)
                                                         gCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtct                                                                                       <  2:727850/149‑1 (MQ=255)
                                                           aaTGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtctgt                                                                                     <  1:717017/149‑1 (MQ=255)
                                                              gCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGa                                                                                  >  4:712074/1‑149 (MQ=255)
                                                               cTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGAt                                                                                 <  2:408381/149‑1 (MQ=255)
                                                               cTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGAt                                                                                 <  2:269362/149‑1 (MQ=255)
                                                                tCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATg                                                                                >  1:755045/1‑149 (MQ=255)
                                                                      acTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAAt                                                                          <  2:857502/149‑1 (MQ=255)
                                                                       cTACGGCGGTAGCCTGAGCGAACTCGCACGGGAGCAACTGCCCGCAGCGGAACAGGCGGCGCTGACGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAGTTATGACTGGCTGTCGATGGAAAAtt                                                                         >  4:680170/1‑149 (MQ=255)
                                                                         aCGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTAc                                                                       <  4:753924/149‑1 (MQ=255)
                                                                               gTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGc                                                                 <  4:77236/149‑1 (MQ=255)
                                                                                        gCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTg                                                        >  2:714388/1‑149 (MQ=255)
                                                                                          gAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGTCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTgcg                                                      <  2:756916/149‑1 (MQ=255)
                                                                                           aaCTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTgcgc                                                     <  1:329192/149‑1 (MQ=255)
                                                                                           aaCTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTgcgc                                                     >  1:580994/1‑149 (MQ=255)
                                                                                              tGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAg                                                  <  1:316711/149‑1 (MQ=255)
                                                                                               ggCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGt                                                 >  3:562685/1‑149 (MQ=255)
                                                                                                  aCGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTAcc                                              >  1:332648/1‑149 (MQ=255)
                                                                                                   cGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtct                                                                                       >  4:192175/1‑107 (MQ=255)
                                                                                                   cGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGActgtct                                                                                       <  3:192175/107‑1 (MQ=255)
                                                                                                   cGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCg                                             <  1:281983/149‑1 (MQ=255)
                                                                                                     ggAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGa                                           <  1:891354/149‑1 (MQ=255)
                                                                                                       aGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATg                                         >  3:21766/1‑149 (MQ=255)
                                                                                                        gCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGa                                           <  3:422891/146‑1 (MQ=255)
                                                                                                        gCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGa                                           >  4:422891/1‑146 (MQ=255)
                                                                                                        gCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGACAGTACCGGTTGa                                        >  1:131552/1‑149 (MQ=255)
                                                                                                        gCAACTGCCCGCTGCGGAACAGGCGGCGCTGGAGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAACATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGa                                        <  3:742305/149‑1 (MQ=255)
                                                                                                             tGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTATGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGGTGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACag                                   >  4:593640/1‑149 (MQ=255)
                                                                                                                  gCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAggagga                              >  3:506970/1‑149 (MQ=255)
                                                                                                                  gCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAggagga                              <  4:119085/149‑1 (MQ=255)
                                                                                                                  gCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCACAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAggagga                              <  2:737603/149‑1 (MQ=255)
                                                                                                                     gCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCa                           <  2:700858/149‑1 (MQ=255)
                                                                                                                      cGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTAt                                                                                               >  2:633713/1‑80 (MQ=255)
                                                                                                                       ggAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAgg                         <  2:732128/149‑1 (MQ=255)
                                                                                                                       ggAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAgg                         <  1:714388/149‑1 (MQ=255)
                                                                                                                        taaCAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCACGCTGTCTCAATTATGACTGTCTGTCAATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAggg                        <  4:655355/148‑1 (MQ=255)
                                                                                                                        gAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAggg                        <  2:790189/149‑1 (MQ=255)
                                                                                                                        gAACAGGCGGCGCTGGCGCAGCTTGCGGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAAGTATGTCTGTCTGTCGGTGGACAATTACGACGGCGCTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAggg                        <  2:858806/149‑1 (MQ=255)
                                                                                                                         aaCAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGa                       >  3:855154/1‑149 (MQ=255)
                                                                                                                          aCAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGAt                      <  4:138226/149‑1 (MQ=255)
                                                                                                                           cAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATg                     <  2:594919/149‑1 (MQ=255)
                                                                                                                            aGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGa                    <  4:777367/149‑1 (MQ=255)
                                                                                                                            aGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGa                    >  1:57501/1‑149 (MQ=255)
                                                                                                                            aGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGa                    <  4:442690/149‑1 (MQ=255)
                                                                                                                              gcggcgCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGTAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGa                    >  2:724905/1‑147 (MQ=255)
                                                                                                                              gcggcgCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGa                    <  1:724905/147‑1 (MQ=255)
                                                                                                                                ggcgCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTATCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCaa                <  4:256750/149‑1 (MQ=255)
                                                                                                                                 gcgcTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAAc               <  4:628332/149‑1 (MQ=255)
                                                                                                                                     ccacgcAGCAAGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGaca           >  2:576449/4‑149 (MQ=255)
                                                                                                                                        cgcAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACTAAGGCGTTCGCTTGCGCCAGTACCGGAGGACACAGGAGGAGCAGGGATGAGCAACGACACGc        >  4:762015/1‑149 (MQ=255)
                                                                                                                                         gcAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAggagga                              <  2:56237/126‑1 (MQ=255)
                                                                                                                                         gcAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAggagga                              >  1:56237/1‑126 (MQ=255)
                                                                                                                                         gcAGCTTGCGGCGTGGTGAATTGTACCGCAACCGGCTAATGAATGGCACTGTCTCGATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGGGCAACGACACGcc       >  4:468186/1‑149 (MQ=255)
                                                                                                                                             cTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCAttt   >  1:281878/1‑149 (MQ=255)
                                                                                                                                              ttGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTg  >  4:195099/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGGGAATTGTGCCGCAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTG  >  NC_000913/1036085‑1036375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: