Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,147,548 T→G 6.3% intergenic (+8/‑73) rpmF → / → plsX 50S ribosomal subunit protein L32/putative phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,147,5480TG 6.3% 470.5 / 17.6 139intergenic (+8/‑73)rpmF/plsX50S ribosomal subunit protein L32/putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (56/73);  new base G (6/3);  total (62/77)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

AAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGC  >  NC_000913/1147400‑1147696
                                                                                                                                                    |                                                                                                                                                    
aaaCGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAt                                                                                                                                                      >  1:558040/1‑149 (MQ=255)
aaaCGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAt                                                                                                                                                      >  3:306881/1‑149 (MQ=255)
         aTGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtgat                                                                                                                                             <  1:215346/149‑1 (MQ=255)
         aTGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtgat                                                                                                                                             <  2:266884/149‑1 (MQ=255)
         aTGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAACTAATCACGCAGATGCGcgat                                                                                                                                             <  2:127648/149‑1 (MQ=255)
            cgtcgtTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGaa                                                                                                                                          <  4:215911/149‑1 (MQ=255)
                 ttCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTa                                                                                                                                     <  4:630261/149‑1 (MQ=255)
                 ttCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTa                                                                                                                                     >  3:737711/1‑149 (MQ=255)
                 ttCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTa                                                                                                                                     <  2:448340/149‑1 (MQ=255)
                   cccATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGt                                                                                                                                   <  2:170402/149‑1 (MQ=255)
                     cATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGa                                                                                                                                 >  4:384012/1‑149 (MQ=255)
                      aTGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAg                                                                                                                                >  1:530102/1‑149 (MQ=255)
                      aTGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAg                                                                                                                                >  1:754645/1‑149 (MQ=255)
                        gACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGa                                                                                                                              <  1:269024/149‑1 (MQ=255)
                        gACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGa                                                                                                                              <  4:804293/149‑1 (MQ=255)
                         aCGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGAt                                                                                                                             <  1:122588/149‑1 (MQ=255)
                           gcgcTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGAttt                                                                                                                           >  3:48293/1‑149 (MQ=255)
                            cgcTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGAtttt                                                                                                                          <  3:557025/149‑1 (MQ=255)
                                  ccGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAg                                                                                                                    <  3:143796/149‑1 (MQ=255)
                                  ccGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAg                                                                                                                    <  4:181667/149‑1 (MQ=255)
                                     cAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCa                                                                                                                 >  3:17888/1‑149 (MQ=255)
                                          aCCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTgg                                                                                                            <  3:177933/149‑1 (MQ=255)
                                          aCCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTgg                                                                                                            <  2:403293/149‑1 (MQ=255)
                                          aCCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTgg                                                                                                            <  2:294638/149‑1 (MQ=255)
                                           ccAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTggg                                                                                                           <  4:15039/149‑1 (MQ=255)
                                           ccAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTggg                                                                                                           <  2:754645/149‑1 (MQ=255)
                                                 tgtctgtAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGa                                                                                                     <  2:530102/149‑1 (MQ=255)
                                                 tgtctgtAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGa                                                                                                     <  3:792562/149‑1 (MQ=255)
                                                         gACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCg                                                                                             >  4:372501/1‑149 (MQ=255)
                                                          aCAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCgg                                                                                            >  3:795447/1‑149 (MQ=255)
                                                          aCAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCgg                                                                                            >  2:387195/1‑149 (MQ=255)
                                                            aaaaCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtgatg                                                                                                                                            >  4:504071/1‑99 (MQ=255)
                                                            aaaaCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtgatg                                                                                                                                            <  3:504071/99‑1 (MQ=255)
                                                            aaaaCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGggc                                                                                          >  2:1372/1‑149 (MQ=255)
                                                             aaaCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGggcg                                                                                         >  1:268325/1‑149 (MQ=255)
                                                                 ttCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcga                                                                                     >  1:40152/1‑149 (MQ=255)
                                                                  tCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcgac                                                                                    <  3:136706/149‑1 (MQ=255)
                                                                  tCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcgac                                                                                    <  4:561955/149‑1 (MQ=255)
                                                                     ggTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGAt                                                                                 <  2:239993/149‑1 (MQ=255)
                                                                       tGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATAc                                                                               >  3:394980/1‑149 (MQ=255)
                                                                        gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGt                                                                                                                                                >  3:447086/1‑83 (MQ=255)
                                                                        gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGt                                                                                                                                                <  4:447086/83‑1 (MQ=255)
                                                                        gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGTTGCCTGGGGaaa                                                                                                       <  3:639899/124‑1 (MQ=255)
                                                                        gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGTTGCCTGGGGaaa                                                                                                       >  4:639899/1‑124 (MQ=255)
                                                                        gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATAcc                                                                              >  4:433360/1‑149 (MQ=255)
                                                                        gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATAcc                                                                              >  1:110776/1‑149 (MQ=255)
                                                                        gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATAcc                                                                              >  4:734949/1‑149 (MQ=255)
                                                                        gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATAcc                                                                              <  3:433360/149‑1 (MQ=255)
                                                                         aaaaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCaaa                                                                                                >  4:555298/1‑130 (MQ=255)
                                                                          aaaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCtt                                                                            <  2:786710/149‑1 (MQ=255)
                                                                          aaaaCACCTGCCTCACCACAACACCGCCGACGGTTACTTCCGCGGCCGCAAAGTCATCGCTAAGTAATCACCCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGGAACTTGGGAAAAAACAAACCGGGCGGCGACGATACCtt                                                                            <  4:394980/149‑1 (MQ=255)
                                                                            aaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGt                                                                                                                                   <  1:727573/92‑1 (MQ=255)
                                                                                 cTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGt                                                                     >  1:278837/1‑149 (MQ=255)
                                                                                  tgagTCACCACATCACTGCCGACGGTTACTACCGCGGCAGCAAGTTCATCGCTAAGTAATCACGCACCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGTAAACTGGGGAAAGACCAAACCGGGCGGAGACGATCCCTTGACACGTc                                                                    <  2:726888/146‑1 (MQ=255)
                                                                                  tGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTc                                                                    <  4:306881/149‑1 (MQ=255)
                                                                                        accacATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAAccc                                                              >  3:731878/1‑149 (MQ=255)
                                                                                         ccacATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCt                                                             >  4:850097/1‑149 (MQ=255)
                                                                                         ccacATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCt                                                             >  4:531766/1‑149 (MQ=255)
                                                                                         ccacATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCt                                                             >  2:455428/1‑149 (MQ=255)
                                                                                          cacaTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTg                                                            <  2:303266/149‑1 (MQ=255)
                                                                                          cacaTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTg                                                            >  2:717986/1‑149 (MQ=255)
                                                                                          cacaTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTCAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTg                                                            >  2:162557/1‑149 (MQ=255)
                                                                                             aTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCg                                                         >  3:351603/1‑149 (MQ=255)
                                                                                             aTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCg                                                         >  3:715726/1‑149 (MQ=255)
                                                                                              tCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGt                                                        >  3:244966/1‑149 (MQ=255)
                                                                                               cACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGtt                                                       <  3:820350/149‑1 (MQ=255)
                                                                                                   gCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGAt                                                   <  2:332006/149‑1 (MQ=255)
                                                                                                       aCGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCa                                               <  4:351603/149‑1 (MQ=255)
                                                                                                       aCGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCa                                               <  2:558040/149‑1 (MQ=255)
                                                                                                          gTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATgg                                            <  3:706991/149‑1 (MQ=255)
                                                                                                            tactacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGa                                          >  1:853816/1‑149 (MQ=255)
                                                                                                             actacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGGTTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAg                                         <  2:268325/149‑1 (MQ=255)
                                                                                                               tacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAggg                                       >  4:581090/1‑149 (MQ=255)
                                                                                                               tacCGCGGCCGCAAGGGCATCGCTAAGTAATCACGCATCCGCGTAATGAAGCGTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGGCGATACCTTGACACCTCTATCCCTGGCGTGAGATGTCATGGGAggg                                       <  4:580007/149‑1 (MQ=255)
                                                                                                                 ccGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGa                                     >  2:726110/1‑149 (MQ=255)
                                                                                                                  cgcgGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGAt                                    >  1:363443/1‑149 (MQ=255)
                                                                                                                     ggCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGAtttt                                 <  1:455428/149‑1 (MQ=255)
                                                                                                                     ggCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGAtttt                                 <  4:679325/149‑1 (MQ=255)
                                                                                                                       ccGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTgg                               <  1:1372/149‑1 (MQ=255)
                                                                                                                         gCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGCGGGGATTTTGGcc                             >  2:171079/1‑149 (MQ=255)
                                                                                                                           aaGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCt                           <  4:62666/149‑1 (MQ=255)
                                                                                                                               tCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCACAGGCAACTgg                                                                                                            <  3:604550/64‑1 (MQ=255)
                                                                                                                                  tCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTgg                                                                                                            <  4:703324/61‑1 (MQ=255)
                                                                                                                                  tCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTgg                                                                                                            >  3:703324/1‑61 (MQ=255)
                                                                                                                                      tAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGt                >  2:520104/1‑149 (MQ=255)
                                                                                                                                        aGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGc              <  1:35070/149‑1 (MQ=255)
                                                                                                                                        aGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGc              <  4:778395/149‑1 (MQ=255)
                                                                                                                                        aGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGc              <  1:673956/149‑1 (MQ=255)
                                                                                                                                         ttcaTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGAt                                                   >  3:562989/4‑111 (MQ=255)
                                                                                                                                         ttcaTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGAt                                                   <  4:562989/108‑1 (MQ=255)
                                                                                                                                         ttcaTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGcc             >  1:534052/4‑149 (MQ=255)
                                                                                                                                         ttcaTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGcc             >  1:880412/4‑149 (MQ=255)
                                                                                                                                         ttcaTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGcc             >  2:612922/4‑149 (MQ=255)
                                                                                                                                         ttcaTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGcc             >  1:691873/4‑149 (MQ=255)
                                                                                                                                          tAATCACTCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCt            >  2:477391/1‑149 (MQ=255)
                                                                                                                                            aTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGtt                                                       >  1:203504/1‑104 (MQ=255)
                                                                                                                                            aTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGtt                                                       <  2:203504/104‑1 (MQ=255)
                                                                                                                                                 gCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCAt     >  3:843283/1‑149 (MQ=255)
                                                                                                                                                  cATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCAtt    <  3:598336/149‑1 (MQ=255)
                                                                                                                                                    tCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGc  <  1:717986/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGC  >  NC_000913/1147400‑1147696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: