Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,151,873 T→A 7.4% intergenic (+22/‑66) acpP → / → fabF acyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,151,8730TA 7.4% 274.8 / 8.9 83intergenic (+22/‑66)acpP/fabFacyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base T (35/41);  new base A (3/3);  total (38/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

TCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGC  >  NC_000913/1151725‑1152018
                                                                                                                                                    |                                                                                                                                                 
tctTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCATGCGGTCGt                                                                                                                                                   >  2:734676/1‑149 (MQ=255)
tctTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGt                                                                                                                                                   >  3:22598/1‑149 (MQ=255)
 ctTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGtt                                                                                                                                                  <  4:354101/149‑1 (MQ=255)
 ctTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGtt                                                                                                                                                  <  1:586380/149‑1 (MQ=255)
  ttGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTc                                                                                                                                                 <  3:470011/149‑1 (MQ=255)
  ttGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTc                                                                                                                                                 <  1:8791/149‑1 (MQ=255)
  ttGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTc                                                                                                                                                 <  3:80259/149‑1 (MQ=255)
     acacCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGAc                                                                                                                                              <  1:741967/149‑1 (MQ=255)
       acCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCg                                                                                                                                            >  2:239691/1‑149 (MQ=255)
       acCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCg                                                                                                                                            >  3:846967/1‑149 (MQ=255)
         cGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGcc                                                                                                                                          <  3:713999/149‑1 (MQ=255)
           ttGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTg                                                                                                                                        >  2:797454/1‑149 (MQ=255)
           ttGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGAACGACCGCCTg                                                                                                                                        <  2:815310/149‑1 (MQ=255)
             gAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGAACGACCGCCTgga                                                                                                                                      <  2:774974/149‑3 (MQ=255)
                cTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGttt                                                                                                                                   >  4:329726/1‑149 (MQ=255)
                   gTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTAt                                                                                                                                <  3:275579/149‑1 (MQ=255)
                     aaTGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCt                                                                                                                              <  3:296269/149‑1 (MQ=255)
                      aTGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCtt                                                                                                                             >  3:585431/1‑149 (MQ=255)
                       tGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCttt                                                                                                                            >  2:531274/1‑149 (MQ=255)
                       tGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCttt                                                                                                                            <  3:718590/149‑1 (MQ=255)
                         gCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCttttt                                                                                                                          <  1:794227/149‑1 (MQ=255)
                               gaagaaGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCGAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCa                                                                                                                    <  3:773400/149‑1 (MQ=255)
                                  gaagaGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTa                                                                                                                 >  3:618344/1‑149 (MQ=255)
                                   aagaGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAg                                                                                                                >  1:12705/1‑149 (MQ=255)
                                     gagTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGAACGACCGCCt                                                                                                                                         >  1:357430/1‑122 (MQ=255)
                                     gagTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGAACGACCGCCt                                                                                                                                         <  2:357430/122‑1 (MQ=255)
                                        tttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCa                                                                                                           >  1:825080/1‑149 (MQ=255)
                                             tctcgagaTTCCGGACGAAGAAGCTGACAAAATCCCCACCGTTCAGGCTGCCATTGATTACATCAACGGCCATCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCAGTAGAATCAttttt                                                                                                      <  4:763831/145‑1 (MQ=255)
                                              aCTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAtttttt                                                                                                     <  1:84373/149‑1 (MQ=255)
                                               cTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtc                                                                                                    <  4:618344/149‑1 (MQ=255)
                                               cTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtc                                                                                                    <  1:134833/149‑1 (MQ=255)
                                               cTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtc                                                                                                    >  2:372472/1‑149 (MQ=255)
                                                tGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtcc                                                                                                   >  4:661402/1‑149 (MQ=255)
                                                tGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtcc                                                                                                   >  1:295308/1‑149 (MQ=255)
                                                tGAGATTCCGGACGAAGAAGCCGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtcc                                                                                                   >  1:256740/1‑149 (MQ=255)
                                                 gagaTTCCGGAGGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAttttttaga                                                                                                  >  3:763831/1‑146 (MQ=255)
                                                      tCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTccctccct                                                                                             <  1:835652/149‑1 (MQ=255)
                                                       ccGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTg                                                                                            <  3:565744/149‑1 (MQ=255)
                                                          gACGAAGAACCTGATTCAACCACCCCCGTACAGGCTGCCATTGATTACATCACCGGCCACCAGGCGCAAGTAAACATCTCCAGGCGGTCGCTCGACCGCCTGAGTTCTATCTTTTTGTCCCACTAGAATCATTTTTTGCCTCCCTggag                                                                                         <  3:476591/149‑1 (MQ=255)
                                                                   gCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgt                                                                                <  2:880812/149‑1 (MQ=255)
                                                                     tGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgtgt                                                                              >  1:584083/1‑149 (MQ=255)
                                                                     tGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgtgt                                                                              >  2:877024/1‑149 (MQ=255)
                                                                     tGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgtgt                                                                              >  3:316961/1‑149 (MQ=255)
                                                                     tGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgt                                                                                <  3:81282/147‑1 (MQ=255)
                                                                     tGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCGTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgt                                                                                >  4:81282/1‑147 (MQ=255)
                                                                     tGAGAAAATCACCACCGTTCAGGATGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgtgt                                                                              >  2:634970/1‑149 (MQ=255)
                                                                       agaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCt                                                                            <  3:661402/149‑1 (MQ=255)
                                                                         aaaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTaa                                                                          >  2:406003/1‑149 (MQ=255)
                                                                                ccaccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTccctccct                                                                                             <  4:787886/123‑1 (MQ=255)
                                                                                ccaccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTccctccct                                                                                             >  3:787886/1‑123 (MQ=255)
                                                                                  accGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCgtgt                                                                 >  1:608173/1‑149 (MQ=255)
                                                                                  accGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCgtgt                                                                 >  2:652316/1‑149 (MQ=255)
                                                                                   ccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtccctccc                                                                                              >  3:422067/1‑119 (MQ=255)
                                                                                   ccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtccctccc                                                                                              <  4:422067/119‑1 (MQ=255)
                                                                                      ttCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGtt                                                             <  1:372472/149‑1 (MQ=255)
                                                                                        cAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTtgt                                                           <  1:366097/149‑1 (MQ=255)
                                                                                         aGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTtgtg                                                          >  4:172860/1‑149 (MQ=255)
                                                                                               tcATTGATTACATGAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATGTTATTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGa                                                    >  4:572372/2‑149 (MQ=255)
                                                                                               ccATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAtt                                                                                                         <  2:242354/96‑1 (MQ=255)
                                                                                               ccATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAtt                                                                                                         >  1:242354/1‑96 (MQ=255)
                                                                                                     aTTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGc                                              >  4:385392/1‑149 (MQ=255)
                                                                                                        aCATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtg                                           >  2:122215/1‑149 (MQ=255)
                                                                                                          aTCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgtt                                         <  2:608173/149‑1 (MQ=255)
                                                                                                          aTCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgtt                                         <  1:531274/149‑1 (MQ=255)
                                                                                                          aTCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgtt                                         <  1:406003/149‑1 (MQ=255)
                                                                                                            cAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgttgt                                       <  3:145667/149‑1 (MQ=255)
                                                                                                            cAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgttgt                                       >  4:111303/1‑149 (MQ=255)
                                                                                                             aaCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtc                                      <  3:572372/149‑1 (MQ=255)
                                                                                                              aCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtct                                     <  2:281150/149‑1 (MQ=255)
                                                                                                              aCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtct                                     >  2:504956/1‑149 (MQ=255)
                                                                                                              aCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtct                                     >  2:602308/1‑149 (MQ=255)
                                                                                                                     ccaGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCg                              <  4:444539/149‑1 (MQ=255)
                                                                                                                         gCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCaa                          >  2:749663/1‑149 (MQ=255)
                                                                                                                         gCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCaa                          <  1:795276/149‑1 (MQ=255)
                                                                                                                                tGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTa                   <  1:122215/149‑1 (MQ=255)
                                                                                                                                  aaCATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTaga                 <  4:311202/149‑1 (MQ=255)
                                                                                                                                  aaCATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTaga                 <  4:74420/149‑1 (MQ=255)
                                                                                                                                  aaCATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTaga                 <  2:797412/149‑1 (MQ=255)
                                                                                                                                      aactcAGGCGGTCGAACGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCt             >  2:728688/5‑149 (MQ=255)
                                                                                                                                       actcAGGCGGTCGAACGACCGCCTGGGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTa            >  3:767389/4‑149 (MQ=255)
                                                                                                                                        tcCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTAc           >  2:133948/1‑149 (MQ=255)
                                                                                                                                          cAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCt         <  3:629043/149‑1 (MQ=255)
                                                                                                                                          cAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCt         <  4:585431/149‑1 (MQ=255)
                                                                                                                                                gTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAg   <  2:151122/149‑1 (MQ=255)
                                                                                                                                                 tCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGc  <  1:239691/149‑1 (MQ=255)
                                                                                                                                                   gTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGaa                                                                                                              <  2:160359/39‑1 (MQ=255)
                                                                                                                                                   gTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGaa                                                                                                              >  1:160359/1‑39 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCTTGACACCGTTGAGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGC  >  NC_000913/1151725‑1152018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: