Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,153,219 G→A 8.7% intergenic (+39/‑81) fabF → / → pabC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,153,2190GA 8.7% 349.9 / 16.3 115intergenic (+39/‑81)fabF/pabC3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base G (53/51);  new base A (2/8);  total (56/59)
Fisher's exact test for biased strand distribution p-value = 9.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 117 total aligned reads displayed.

GGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGA  >  NC_000913/1153072‑1153367
                                                                                                                                                   |                                                                                                                                                    
ggATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGc                                                                                                                                                     >  1:657050/1‑149 (MQ=255)
ggATTTCGTACCGAACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTTTGTGTAACTCCTTCGGCTTCGGAGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGc                                                                                                                                                     >  3:588424/1‑149 (MQ=255)
  aTTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCt                                                                                                                                                   <  1:311594/149‑1 (MQ=255)
   tttCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCtt                                                                                                                                                  <  1:886348/149‑1 (MQ=255)
    ttCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCAAGCGGACCttt                                                                                                                                                 <  1:847440/149‑1 (MQ=255)
    ttCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTGCGGCTTCGGTGGCGCTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCAAGCGGACCttt                                                                                                                                                 <  2:681872/149‑1 (MQ=255)
    ttCGTACCACACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCAAGCGGACCttt                                                                                                                                                 <  4:458460/149‑1 (MQ=255)
      cGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGATTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCttttt                                                                                                                                               >  4:522126/1‑149 (MQ=255)
         aCCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCt                                                                                                                                            >  2:669142/1‑149 (MQ=255)
          ccGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCtt                                                                                                                                           <  3:551988/149‑1 (MQ=255)
           cGCACGAAGAGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTCATGGTTCTATGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCAAGCGGACCttttataagg                                                                                                                                          <  4:208400/149‑7 (MQ=255)
            gCACGAAGCGCGTCAGGTTAGCGGAATGGAATACCCTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACGCTTATAAAAGGCCCGCAAGCGGACCtgttataaggg                                                                                                                                         <  2:426367/149‑8 (MQ=255)
            gCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGCCCGCAAGCGGACCttttataaggg                                                                                                                                         <  2:831764/149‑8 (MQ=255)
             cACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGc                                                                                                                                        <  2:737193/149‑1 (MQ=255)
                 aaGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCtttt                                                                                                                                    <  2:380367/149‑1 (MQ=255)
                  aGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTa                                                                                                                                   <  3:593225/149‑1 (MQ=255)
                  aGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTa                                                                                                                                   <  2:94216/149‑1 (MQ=255)
                  aGCGCGTCAGGTTACCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTa                                                                                                                                   <  1:118520/149‑1 (MQ=255)
                     gcgTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTc                                                                                                                                <  4:496180/149‑1 (MQ=255)
                         cAGGTTAGCGGAAAGGGATACACTATGTGCAACTCCTTCGGCTTTGGTGGAACTAATGGTACTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCGGAc                                                                                                                            <  4:250478/149‑1 (MQ=255)
                                   gaatggaatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTa                                                                                                                  <  3:222771/149‑1 (MQ=255)
                                        gaatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGaa                                                                                                             <  1:649222/149‑1 (MQ=255)
                                        gaatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGaa                                                                                                             >  2:366239/1‑149 (MQ=255)
                                         aatACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAAc                                                                                                            >  3:875454/1‑149 (MQ=255)
                                             cacTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACAtgc                                                                                                        <  1:458478/149‑1 (MQ=255)
                                               ctctGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACAtgctg                                                                                                      >  1:520418/1‑149 (MQ=255)
                                                tctGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACAtgctgc                                                                                                     >  2:68110/1‑149 (MQ=255)
                                                tctGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACAtgctgc                                                                                                     <  2:520418/149‑1 (MQ=255)
                                                    tgtAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCcaca                                                                                                 >  1:163832/1‑149 (MQ=255)
                                                          tCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACa                                                                                           >  3:875996/1‑149 (MQ=255)
                                                          tCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACa                                                                                           >  2:270777/1‑149 (MQ=255)
                                                            cttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAAt                                                                                         >  1:180585/1‑149 (MQ=255)
                                                            cttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAAt                                                                                         >  4:74133/1‑149 (MQ=255)
                                                             ttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAAtt                                                                                        >  4:659397/1‑149 (MQ=255)
                                                             ttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAAtt                                                                                        >  2:803582/1‑149 (MQ=255)
                                                             ttcggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAAtt                                                                                        >  1:641219/1‑149 (MQ=255)
                                                                ggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtctc                                                                                     >  3:396969/1‑149 (MQ=255)
                                                                ggcttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtctc                                                                                     <  1:533203/149‑1 (MQ=255)
                                                                ggctgcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGTCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtctc                                                                                     <  3:312939/149‑1 (MQ=255)
                                                                   ttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCg                                                                                                                    >  2:415683/1‑115 (MQ=255)
                                                                   ttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCg                                                                                                                    <  1:415683/115‑1 (MQ=255)
                                                                   ttcggTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGa                                                                                  <  2:562571/149‑1 (MQ=255)
                                                                        tGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAgg                                                                             <  2:383685/149‑1 (MQ=255)
                                                                            aCTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGcc                                                                         <  1:233711/149‑1 (MQ=255)
                                                                              tAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGCCTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtct                                                                                      <  2:19470/134‑1 (MQ=255)
                                                                              tAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATtct                                                                                      >  1:19470/1‑134 (MQ=255)
                                                                                   gTTCTTTGATCTTTGAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGt                                                                  >  3:586199/1‑149 (MQ=255)
                                                                                   gTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCTACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGt                                                                  >  3:50972/1‑149 (MQ=255)
                                                                                   gTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGt                                                                  >  3:381128/1‑149 (MQ=255)
                                                                                   gTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGt                                                                  >  4:30396/1‑149 (MQ=255)
                                                                                   gTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGt                                                                  >  1:291271/1‑149 (MQ=255)
                                                                                    ttCTTTGGTCTTGAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCTGCTTGCGGTCCTTGTTTCTTAGCTTGTATTCCGTCTTGTTCCGTAGTGAGCATGCTGCCACACTAACAATCCTCTGCTATCGAGCCGATATGtt                                                                 >  4:844996/1‑149 (MQ=255)
                                                                                       tttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTTCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                              >  2:838817/1‑149 (MQ=255)
                                                                                       tttGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCtt                                                              >  2:353853/1‑149 (MQ=255)
                                                                                          gATCTTTAAAAAGATCTAAGTTGTCATTTTCCTCCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCttaat                                                           <  2:268392/149‑1 (MQ=255)
                                                                                           aTCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCttaatt                                                          >  3:843078/1‑149 (MQ=255)
                                                                                            tCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATTCTGCCACACTAACAATTCTCTGTTAAGGAGCCGGTATGTTCttaatta                                                         >  2:471338/1‑149 (MQ=255)
                                                                                            tCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGAAGCCGGTATGTTCttaatta                                                         >  3:327991/1‑149 (MQ=255)
                                                                                              tttAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAAc                                                       <  4:381128/149‑1 (MQ=255)
                                                                                              tttAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAAc                                                       >  3:275582/1‑149 (MQ=255)
                                                                                              tttAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAAc                                                       <  2:180585/149‑1 (MQ=255)
                                                                                                  aaaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAAtt                                                                                        >  2:545666/1‑112 (MQ=255)
                                                                                                  aaaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAAtt                                                                                        <  1:545666/112‑1 (MQ=255)
                                                                                                   aaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGtt                                                                 <  4:519684/134‑1 (MQ=255)
                                                                                                   aaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGtt                                                                 >  3:519684/1‑134 (MQ=255)
                                                                                                   aaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCa                                                  >  1:29698/1‑149 (MQ=255)
                                                                                                   aaaGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCa                                                  <  4:791314/149‑1 (MQ=255)
                                                                                                       aTCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATaa                                               <  3:655030/148‑1 (MQ=255)
                                                                                                       aTCTAAGTTGTCATTATCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATaa                                               >  4:655030/1‑148 (MQ=255)
                                                                                                        tCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGc                                             >  1:116175/1‑149 (MQ=255)
                                                                                                         cTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCa                                            <  1:270777/149‑1 (MQ=255)
                                                                                                          tAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAg                                           <  1:669142/149‑1 (MQ=255)
                                                                                                          tAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGACCCGGTATGTTCTTAATTAACGGTCATAAGCAg                                           >  3:260708/1‑149 (MQ=255)
                                                                                                              ttGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAAt                                       >  1:856086/1‑149 (MQ=255)
                                                                                                              ttGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATCAACGGTCATAAGCAGGAAt                                       >  4:146599/1‑149 (MQ=255)
                                                                                                               tGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATc                                      <  4:588424/149‑1 (MQ=255)
                                                                                                                 tCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGc                                    <  4:535865/149‑1 (MQ=255)
                                                                                                                    ttttCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTgg                                 <  3:522126/149‑1 (MQ=255)
                                                                                                                    tattCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTgg                                 >  2:712074/3‑149 (MQ=255)
                                                                                                                      ttCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCa                               >  2:660239/1‑149 (MQ=255)
                                                                                                                         cACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTa                            >  4:301471/1‑149 (MQ=255)
                                                                                                                          aCCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTaa                           >  4:275218/1‑149 (MQ=255)
                                                                                                                          aCCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTaa                           >  4:618054/1‑149 (MQ=255)
                                                                                                                          aCCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTaa                           >  1:625100/1‑149 (MQ=255)
                                                                                                                            taagaaaaaaGGCCCGCAAGCGGACCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGc                         >  2:149840/7‑149 (MQ=255)
                                                                                                                            ccTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGc                         >  1:622783/1‑149 (MQ=255)
                                                                                                                             cTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCg                        >  4:489736/1‑149 (MQ=255)
                                                                                                                                 taAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCg                    <  4:477044/149‑1 (MQ=255)
                                                                                                                                 taAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATCAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCg                    <  3:146599/149‑1 (MQ=255)
                                                                                                                                  aaaaGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCgg                   <  4:73430/149‑1 (MQ=255)
                                                                                                                                  aaaaGGCCCGCAAGCGGACCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCgg                   >  3:58749/1‑149 (MQ=255)
                                                                                                                                     aGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCa                >  4:836666/1‑149 (MQ=255)
                                                                                                                                       gTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAAc              <  3:74133/149‑1 (MQ=255)
                                                                                                                                       gTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAAc              <  1:366239/149‑1 (MQ=255)
                                                                                                                                       gTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAAc              <  4:657745/149‑1 (MQ=255)
                                                                                                                                          cGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCa           <  4:356767/149‑1 (MQ=255)
                                                                                                                                              tGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGttt       <  4:192998/149‑1 (MQ=255)
                                                                                                                                               gCGGACCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTg      <  1:149840/149‑1 (MQ=255)
                                                                                                                                                   gCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGa  <  1:300209/149‑1 (MQ=255)
                                                                                                                                                   gCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGa  >  3:344243/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTAAGTTGTCATTTTCCACCCTTATAAAAGGTCCGCTTGCGGGCCTTTTTTCTTAGCTTTTATTCCGACTTGTTCCGTAGTGAACATGCTGCCACACTAACAATTCTCTGATAAGGAGCCGGTATGTTCTTAATTAACGGTCATAAGCAGGAATCGCTGGCAGTAAGCGATCGGGCAACGCAGTTTGGTGA  >  NC_000913/1153072‑1153367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: