Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,603,724 A→C 7.3% T236P (ACC→CCC)  lsrC → autoinducer 2 import system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,603,7240AC 7.3% 344.0 / 19.5 109T236P (ACC→CCC) lsrCautoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand):  ref base A (44/57);  new base C (2/6);  total (46/63)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-02

Only 100 of 111 total aligned reads displayed.

TTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATT  >  NC_000913/1603576‑1603870
                                                                                                                                                    |                                                                                                                                                  
ttATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGa                                                                                                                                                    <  2:1872/149‑1 (MQ=255)
ttATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGa                                                                                                                                                    <  1:508665/149‑1 (MQ=255)
 tATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGAc                                                                                                                                                   <  2:411814/149‑1 (MQ=255)
  aTGCCACGGGCGATAATTTACAGAGCGCTCGTCAACTGGGCGTTAGTACTGAAGCCATTCGCAGTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGAGTGGTTTTATCCCCAACCAGAcc                                                                                                                                                  >  2:139788/1‑149 (MQ=255)
     ccACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGt                                                                                                                                               >  3:618184/1‑149 (MQ=255)
             gATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTTGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCt                                                                                                                                       >  4:511071/1‑149 (MQ=255)
             gATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCt                                                                                                                                       <  3:446038/149‑1 (MQ=255)
              aTAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTTGTTGAACGGCTGCATGGCGGCACTGGCGGGAAGTGTGTTTGCTTCGCAGATTGGTTTTAGTCCCAACCAGCCCGGTACCGGTCTg                                                                                                                                      <  2:470465/149‑1 (MQ=255)
              aTAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTg                                                                                                                                      <  3:66802/149‑1 (MQ=255)
               tAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTgg                                                                                                                                     <  2:797382/149‑1 (MQ=255)
               tAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTgg                                                                                                                                     >  4:289012/1‑149 (MQ=255)
               tAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTgg                                                                                                                                     <  4:564745/149‑1 (MQ=255)
                aaTTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGga                                                                                                                                    <  1:198892/149‑1 (MQ=255)
                aaTTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGga                                                                                                                                    <  3:606415/149‑1 (MQ=255)
                aaTTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGga                                                                                                                                    <  2:88016/149‑1 (MQ=255)
                aaTTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGga                                                                                                                                    <  2:749231/149‑1 (MQ=255)
                  tttACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGgaga                                                                                                                                  <  4:728384/149‑1 (MQ=255)
                    tACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTggttggg                                                                                                                                <  3:660252/149‑6 (MQ=255)
                     aCAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTggttgggg                                                                                                                               <  4:390033/149‑7 (MQ=255)
                       aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                             <  2:349148/149‑1 (MQ=255)
                       aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                             <  4:618184/149‑1 (MQ=255)
                       aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                             <  3:419057/149‑1 (MQ=255)
                       aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                             >  3:681536/1‑149 (MQ=255)
                       aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                             <  4:757239/149‑1 (MQ=255)
                       aGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                             <  4:222713/149‑1 (MQ=255)
                       aGGGCGCTCGTCAACTGGGCGTTCGTACCGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGAGTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                             <  4:238471/149‑1 (MQ=255)
                           cgcTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAAt                                                                                                                        <  2:328725/149‑1 (MQ=255)
                                gTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCa                                                                                                                    >  3:306963/1‑149 (MQ=255)
                                 tCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAg                                                                                                                   >  3:664936/1‑149 (MQ=255)
                                  cAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGc                                                                                                                  <  1:139788/149‑1 (MQ=255)
                                    aCTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAAGGGCTGCATGGCGTCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCt                                                                                                                >  4:647459/1‑149 (MQ=255)
                                      tGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                             <  2:628231/134‑1 (MQ=255)
                                      tGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGaaa                                                                                                                             >  1:628231/1‑134 (MQ=255)
                                      tGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGc                                                                                                              >  1:113588/1‑149 (MQ=255)
                                      tGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGc                                                                                                              <  2:83595/149‑1 (MQ=255)
                                         gCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCACCCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTg                                                                                                           <  2:192852/149‑1 (MQ=255)
                                         gCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTg                                                                                                           >  4:420346/1‑149 (MQ=255)
                                              cGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTggg                                                                                                      <  3:410206/149‑1 (MQ=255)
                                                    gAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCAt                                                                                                <  3:647459/149‑1 (MQ=255)
                                                     aaGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCAtt                                                                                               >  2:833327/1‑149 (MQ=255)
                                                       gCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGTGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTa                                                                                              >  4:146590/1‑148 (MQ=255)
                                                       gCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTa                                                                                              <  3:146590/148‑1 (MQ=255)
                                                          aTTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGttt                                                                                          >  2:354019/1‑149 (MQ=255)
                                                          aTTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGttt                                                                                          >  3:781425/1‑149 (MQ=255)
                                                           ttCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTg                                                                                         >  1:614694/1‑149 (MQ=255)
                                                              gCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTc                                                                                      >  2:436824/1‑149 (MQ=255)
                                                                aTTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGTATTAGTTTGCTCgg                                                                                    <  3:68028/149‑1 (MQ=255)
                                                                 aaagaataTTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCggt                                                                                   <  2:254140/142‑1 (MQ=255)
                                                                    tgGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCggtggt                                                                                >  3:520323/1‑149 (MQ=255)
                                                                          tttCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGt                                                                          <  3:511071/149‑1 (MQ=255)
                                                                           ttCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTg                                                                         >  1:811112/1‑149 (MQ=255)
                                                                            tCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGTGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGGTCCGGTGc                                                                        >  4:68078/1‑149 (MQ=255)
                                                                             cGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCg                                                                       <  1:607848/149‑1 (MQ=255)
                                                                              gTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTAGGTGGTTCCGGTGCGa                                                                      >  3:561147/1‑149 (MQ=255)
                                                                                tGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATc                                                                    <  1:541772/149‑1 (MQ=255)
                                                                                  aaCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTggt                                                                                                                                    <  2:604381/83‑2 (MQ=255)
                                                                                  aaCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGCCCGGTACCGGTCTggt                                                                                                                                    >  1:604381/1‑82 (MQ=255)
                                                                                  aaCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCAt                                                                  <  1:163449/149‑1 (MQ=255)
                                                                                   aCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCAtt                                                                 <  2:787355/149‑1 (MQ=255)
                                                                                    cGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTg                                                                <  1:248236/149‑1 (MQ=255)
                                                                                           aTGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAg                                                                                                                   <  1:19426/91‑1 (MQ=255)
                                                                                           aTGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGt                                                         >  1:567263/1‑149 (MQ=255)
                                                                                            tGGCGGCAATGGCGGTGATTGTGTTTGTTTCGCAGACTGGATTTATCCCCAACCAGACCGGTACCGGGCCGGGGATTAAAGCAATTGCAGCCAGCGTCCTCCGCGGAACTCGTTTGCTCGGTGGATCCGGTGCGAACATCGGTGCCGTa                                                        >  2:132084/1‑149 (MQ=255)
                                                                                                      ggCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCAt                                              <  4:156536/149‑1 (MQ=255)
                                                                                                      ggCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCAt                                              <  1:499755/149‑1 (MQ=255)
                                                                                                         gggAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGt                                           >  3:135395/1‑149 (MQ=255)
                                                                                                         gggAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGt                                           >  1:426322/1‑149 (MQ=255)
                                                                                                               tgtgTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGa                                     <  3:289012/149‑1 (MQ=255)
                                                                                                               tgtgTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCAGTGCAATCATTGGTGCGGTACTCGGCGCATGGTTCCTGa                                     <  4:51043/149‑1 (MQ=255)
                                                                                                                    ttGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAg                                <  2:541491/149‑1 (MQ=255)
                                                                                                                         tCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGa                           >  2:97658/1‑149 (MQ=255)
                                                                                                                           gCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATa                         <  4:781425/149‑1 (MQ=255)
                                                                                                                              gATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGc                                                                                        <  2:870758/83‑1 (MQ=255)
                                                                                                                              gATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGc                                                                                        >  1:870758/1‑83 (MQ=255)
                                                                                                                              gATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCg                      >  4:529759/1‑149 (MQ=255)
                                                                                                                               aTTGGTTTTATCCCCAACCTGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTgg                                                                                                       >  4:748515/1‑67 (MQ=255)
                                                                                                                               aTTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTgg                                                                                                       <  3:748515/67‑1 (MQ=255)
                                                                                                                                ttGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTa                    <  1:801604/149‑1 (MQ=255)
                                                                                                                                   gTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTg                 <  4:746815/149‑1 (MQ=255)
                                                                                                                                    ttttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGgc                                                  <  4:783799/115‑1 (MQ=255)
                                                                                                                                    ttttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGgc                                                  >  3:783799/1‑115 (MQ=255)
                                                                                                                                    ttttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                >  2:211208/1‑149 (MQ=255)
                                                                                                                                    ttttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                >  3:524936/1‑149 (MQ=255)
                                                                                                                                    ttttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                >  4:161278/1‑149 (MQ=255)
                                                                                                                                    ttttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                >  4:505501/1‑149 (MQ=255)
                                                                                                                                    ttttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                >  2:676015/1‑149 (MQ=255)
                                                                                                                                      ttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTg              <  3:459325/149‑1 (MQ=255)
                                                                                                                                        aTCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGc             <  1:851191/148‑1 (MQ=255)
                                                                                                                                        aTCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGc             >  2:851191/1‑148 (MQ=255)
                                                                                                                                           cccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGCTCATTGGTGCGGTACTCGGCGCATGGTTCCTGACTCAGATCGATAGCGTACTGGTCCTGtt         <  4:561147/149‑1 (MQ=255)
                                                                                                                                           cccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt         <  3:211774/149‑1 (MQ=255)
                                                                                                                                           cccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt         >  3:459341/1‑149 (MQ=255)
                                                                                                                                            ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTcc                                        >  2:193212/1‑117 (MQ=255)
                                                                                                                                            ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTcc                                        <  1:193212/117‑1 (MQ=255)
                                                                                                                                            ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTg        >  1:755863/1‑149 (MQ=255)
                                                                                                                                            ccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTg        <  1:354019/149‑1 (MQ=255)
                                                                                                                                            atctccAGCCCGGTACCGGTCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTg        >  1:163925/5‑149 (MQ=255)
                                                                                                                                             cAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTgc       <  4:483888/149‑1 (MQ=255)
                                                                                                                                             cAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTgc       <  2:811112/149‑1 (MQ=255)
                                                                                                                                                  aGACCGGTATCGGGCTGGAGATTGAAGCAGTTGCAGTCTGCGTGCTGGGCGGCCTTAGGTTTCTCTGTGGTTCCGGTTCGTTCATTGGGGCGGTATTCGGCCCATGGTTCCTGACGCAGATCGTTAGAGTAGTGGTGCGGTTgacaatt  >  4:220836/1‑143 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTATGCCACGGGCGATAATTTACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATT  >  NC_000913/1603576‑1603870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: