Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,608,704 A→G 14.0% S107P (TCG→CCG)  yneE ← bestrophin family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,608,7040AG14.0% 222.7 / 23.0 84S107P (TCG→CCG) yneEbestrophin family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (37/35);  new base G (2/10);  total (39/45)
Fisher's exact test for biased strand distribution p-value = 3.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTG  >  NC_000913/1608558‑1608852
                                                                                                                                                  |                                                                                                                                                    
tGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAAt                                                                                                                                                    >  1:250333/1‑149 (MQ=255)
      gTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCa                                                                                                                                              >  2:485185/1‑149 (MQ=255)
       ttCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaa                                                                                                                                             <  1:657753/149‑1 (MQ=255)
        tCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaag                                                                                                                                            <  3:302110/149‑2 (MQ=255)
        tCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaag                                                                                                                                            <  4:298502/149‑2 (MQ=255)
         cGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagt                                                                                                                                           <  2:570849/149‑3 (MQ=255)
         cGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagt                                                                                                                                           <  1:341476/149‑3 (MQ=255)
         cGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagt                                                                                                                                           <  1:210569/149‑3 (MQ=255)
          gAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagt                                                                                                                                           >  3:324768/1‑146 (MQ=255)
          gAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagt                                                                                                                                           <  4:324768/148‑3 (MQ=255)
          gAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAGTCCGGCAAt                                                                                                                                            <  1:560171/147‑1 (MQ=255)
          gAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAGTCCGGCAAt                                                                                                                                            >  2:560171/1‑147 (MQ=255)
           aaGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCCGCCGGGCAAACCCCCTTTCACTTGCCGGATCCGGCAATGTc                                                                                                                                         <  4:601160/149‑1 (MQ=255)
           aaGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagtgt                                                                                                                                         <  2:315106/149‑5 (MQ=255)
             gCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagtgtaa                                                                                                                                       <  2:15559/149‑7 (MQ=255)
             gCCAGTACACGATGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGt                                                                                                                                       >  1:772083/1‑149 (MQ=255)
                 gTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGttttt                                                                                                                                   >  2:741099/1‑149 (MQ=255)
                 gTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGttttt                                                                                                                                   <  2:792334/149‑1 (MQ=255)
                   acacGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTAc                                                                                                                                 <  2:250333/149‑1 (MQ=255)
                    cacGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTAcc                                                                                                                                >  1:483607/1‑149 (MQ=255)
                       gCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTcg                                                                                                                             >  2:464873/1‑149 (MQ=255)
                         tGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTcgcg                                                                                                                           >  4:151359/1‑149 (MQ=255)
                             gATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGt                                                                                                                       >  2:691960/1‑149 (MQ=255)
                             gATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGt                                                                                                                       >  2:305129/1‑149 (MQ=255)
                              aTCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTa                                                                                                                      >  4:89471/1‑149 (MQ=255)
                                 ttCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACg                                                                                                                   >  4:671665/1‑149 (MQ=255)
                                  tCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATTCGGCAATGTCGTTTTTACCTCCCGCAGTAACGa                                                                                                                  <  2:168807/149‑1 (MQ=255)
                                        ttGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGtttt                                                                                                                                    <  3:800751/125‑1 (MQ=255)
                                        ttGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGtttt                                                                                                                                    >  4:800751/1‑125 (MQ=255)
                                        ttGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGa                                                                                                            >  4:172595/1‑149 (MQ=255)
                                          gagaTAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTc                                                                                                                                         <  3:482664/118‑1 (MQ=255)
                                          gagaTAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTc                                                                                                                                         >  4:482664/1‑118 (MQ=255)
                                            gaTAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCa                                                                                                        >  4:13066/1‑149 (MQ=255)
                                              tAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAat                                                                                                      <  3:671665/149‑1 (MQ=255)
                                                  gAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAatcatc                                                                                                  <  2:483607/149‑1 (MQ=255)
                                                    gCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCaa                                                                                                <  3:83221/149‑1 (MQ=255)
                                                    gCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCaa                                                                                                <  4:631906/149‑1 (MQ=255)
                                                     ccAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAAGCAATCATCAAc                                                                                               >  4:686924/1‑149 (MQ=255)
                                                      cAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACt                                                                                              >  2:146054/1‑149 (MQ=255)
                                                       aGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTg                                                                                             >  4:506404/1‑149 (MQ=255)
                                                          aCTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGccc                                                                                          >  2:142901/1‑149 (MQ=255)
                                                          aCTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGccc                                                                                          <  1:142901/149‑1 (MQ=255)
                                                               cGCCTGTGTCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCaaa                                                                                     <  2:772083/149‑1 (MQ=255)
                                                                     tgGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCaa                                                                                                       <  3:265908/125‑1 (MQ=255)
                                                                     tgGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCaa                                                                                                       >  4:265908/1‑125 (MQ=255)
                                                                     tgGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTc                                                                               <  1:271578/149‑1 (MQ=255)
                                                                     tgGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTc                                                                               <  4:578753/149‑1 (MQ=255)
                                                                     tgGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTc                                                                               <  1:574606/149‑1 (MQ=255)
                                                                     tgGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTc                                                                               <  3:873098/149‑1 (MQ=255)
                                                                          gTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCt                                                                          <  3:168023/149‑1 (MQ=255)
                                                                           tttGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCGGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCtt                                                                         >  3:727329/1‑149 (MQ=255)
                                                                              gCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCaa                                                                      >  2:234801/1‑149 (MQ=255)
                                                                                     gTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTATTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCAAAAGTTTTCGCGCTTCAACGTAACg                                                               >  2:837157/1‑149 (MQ=255)
                                                                                      tCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACCCTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACgg                                                              <  2:210984/149‑1 (MQ=255)
                                                                                      tCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACgg                                                              <  1:685384/149‑1 (MQ=255)
                                                                                        aTGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGc                                                            <  1:217639/149‑1 (MQ=255)
                                                                                        aTGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGc                                                            <  3:344939/149‑1 (MQ=255)
                                                                                            gTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTAc                                                        >  2:373900/1‑149 (MQ=255)
                                                                                            gTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTAc                                                        >  2:269909/1‑149 (MQ=255)
                                                                                             tAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTAcc                                                       <  1:146054/149‑1 (MQ=255)
                                                                                                      ggCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCattat                                              >  4:549792/1‑149 (MQ=255)
                                                                                                        cGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCaaa                                                                                     <  3:154336/108‑1 (MQ=255)
                                                                                                        cGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCaaa                                                                                     >  4:154336/1‑108 (MQ=255)
                                                                                                         gAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTAc                                           >  2:618180/1‑149 (MQ=255)
                                                                                                          aaaGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACg                                          >  1:292202/1‑149 (MQ=255)
                                                                                                                 tttGCAGCCGGGCAAACTCCCTTACGCTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCa                                   >  2:5085/1‑149 (MQ=255)
                                                                                                                 tttGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCa                                   >  3:832161/1‑149 (MQ=255)
                                                                                                                  ttGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCaa                                  <  4:832161/149‑1 (MQ=255)
                                                                                                                         cGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAat                           <  1:687046/149‑1 (MQ=255)
                                                                                                                            gCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggc                        <  3:36610/149‑1 (MQ=255)
                                                                                                                            gCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggc                        <  3:13066/149‑1 (MQ=255)
                                                                                                                              aaaCTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACTCGGCATTATTACGAAAACCAAGAAAAatggcga                      <  2:17014/149‑1 (MQ=255)
                                                                                                                               aaCTCCCTTACACTTGCCGAATCCGGTAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggcgat                     <  1:719204/149‑1 (MQ=255)
                                                                                                                                  tCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggcgatggc                  <  2:168329/149‑1 (MQ=255)
                                                                                                                                  tCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggcgatggc                  >  3:361945/1‑149 (MQ=255)
                                                                                                                                  tCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggcgatggc                  <  1:464873/149‑1 (MQ=255)
                                                                                                                                    ccTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggc                        <  2:569923/141‑1 (MQ=255)
                                                                                                                                    ccTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggc                        >  1:569923/1‑141 (MQ=255)
                                                                                                                                         cacTTGCCGAATCCGGCAATTTCGTTTTTACCTCGCGCAGTAACGACCGGGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCg           <  3:625101/149‑1 (MQ=255)
                                                                                                                                          acTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggc                        <  3:417966/135‑1 (MQ=255)
                                                                                                                                          acTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatggc                        >  4:417966/1‑135 (MQ=255)
                                                                                                                                          acTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCga          >  2:119303/1‑149 (MQ=255)
                                                                                                                                          acTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCga          >  3:532907/1‑149 (MQ=255)
                                                                                                                                            ttGCCGGATTCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCgaga        >  3:727267/1‑149 (MQ=255)
                                                                                                                                                  aaTCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTg  >  3:761541/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTG  >  NC_000913/1608558‑1608852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: