Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,666,470	C→A	5.7%	L386I (CTA→ATA)	clcB →	H(+)/Cl(‑) exchange transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,666,470	0	C	A	5.7%	232.6 / 2.6	67	L386I (CTA→ATA)	clcB	H(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base C (26/37);  new base A (2/2);  total (28/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

TGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATAA  >  NC_000913/1666322‑1666617                                                                                                                                                     |                                                                                                                                                    tGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGc                                                                                                                                                     <  3:344959/149‑1 (MQ=255) tGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGc                                                                                                                                                     <  3:8481/149‑1 (MQ=255)         gCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTcccc                                                                                                                                             <  3:827397/149‑1 (MQ=255)           cTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCgg                                                                                                                                           <  3:696052/149‑1 (MQ=255)            tGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGt                                                                                                                                          >  4:508628/1‑149 (MQ=255)                 ttATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTatt                                                                                                                                     <  1:582810/149‑1 (MQ=255)                    tGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgat                                                                                                                                  >  3:101479/1‑149 (MQ=255)                       ttCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGc                                                                                                                               <  4:330620/149‑1 (MQ=255)                         cccTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCt                                                                                                                             >  3:223104/1‑149 (MQ=255)                         cccTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCt                                                                                                                             <  3:417298/149‑1 (MQ=255)                            tGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCg                                                                                                                          >  3:765712/1‑149 (MQ=255)                               tGGCGAAGAAATTACACTTTTACTCGGATTGTCGGGGATGGCGACACTGTTGGCGCCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTaa                                                                                                                       <  2:315713/149‑1 (MQ=255)                                          ttACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTa                                                                                                            >  2:332595/1‑149 (MQ=255)                                           tACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTaa                                                                                                           >  3:875125/1‑149 (MQ=255)                                            acacTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAAt                                                                                                          <  4:875125/149‑1 (MQ=255)                                               cTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTc                                                                                                       <  4:439724/149‑1 (MQ=255)                                                ttttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTat                                                                                                                                      <  2:797864/117‑1 (MQ=255)                                                ttttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTat                                                                                                                                      >  1:797864/1‑117 (MQ=255)                                                ttttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcg                                                                                                      <  2:716246/149‑1 (MQ=255)                                                ttttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcg                                                                                                      >  4:29771/1‑149 (MQ=255)                                                 tttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgc                                                                                                     <  4:723952/149‑1 (MQ=255)                                                 tttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgc                                                                                                     <  2:509510/149‑1 (MQ=255)                                                 tttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgc                                                                                                     <  4:343757/149‑1 (MQ=255)                                                 tttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgc                                                                                                     <  3:508628/149‑1 (MQ=255)                                                      tCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACg                                                                                                >  2:283782/1‑149 (MQ=255)                                                               ccGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGt                                                                                       >  4:550877/1‑149 (MQ=255)                                                                cGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTg                                                                                      >  1:301455/1‑149 (MQ=255)                                                                 gggATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGa                                                                                     <  3:42915/149‑1 (MQ=255)                                                                 gggATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGa                                                                                     <  4:209933/149‑1 (MQ=255)                                                                 gggATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGa                                                                                     <  3:207201/149‑1 (MQ=255)                                                                 gggATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGa                                                                                     <  1:200075/149‑1 (MQ=255)                                                                  ggATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGAc                                                                                    >  2:75465/1‑149 (MQ=255)                                                                   gATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGAct                                                                                   <  1:378063/149‑1 (MQ=255)                                                                         gACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatct                                                                             >  3:860555/1‑149 (MQ=255)                                                                         gACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatct                                                                             >  3:469025/1‑149 (MQ=255)                                                                          acacTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatcta                                                                            <  3:443703/149‑1 (MQ=255)                                                                           cacTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTAc                                                                           <  3:671160/149‑1 (MQ=255)                                                                                   gcggcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGca                                                                   >  3:762281/1‑149 (MQ=255)                                                                                      gcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATTTTCCGCCAGCacac                                                                <  3:66591/149‑1 (MQ=255)                                                                                      gcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac                                                                <  4:706448/149‑1 (MQ=255)                                                                                        gACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCATGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTAGCTACCGCCAGCACACTg                                                              >  2:251348/1‑149 (MQ=255)                                                                                            acgcacgcGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCATGACTCTATCTACCGCCAGCACACTGCgca                                                          <  3:851190/149‑1 (MQ=255)                                                                                                 cgcgCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATa                                                     <  1:534404/149‑1 (MQ=255)                                                                                                 cgcgCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATa                                                     <  3:29771/149‑1 (MQ=255)                                                                                                    gcCGACTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCATCTACTCCCCGGTTTATTGATTGCCTGCGTATTTGGGTCCGTAATTTCGCGGACGTTACACCGTGACTCTATATACCGCCAGCACACTGCGCAGCATAggt                                                  >  4:496911/1‑147 (MQ=255)                                                                                                          tATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCgg                                            <  1:332595/149‑1 (MQ=255)                                                                                                            tGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGAt                                          <  1:283782/149‑1 (MQ=255)                                                                                                            tGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGAt                                          <  2:708551/149‑1 (MQ=255)                                                                                                            tGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGAt                                          <  2:22972/149‑1 (MQ=255)                                                                                                              tctaCGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGCCGGTAGTTTCGCGGACGTTACAACGTGACTCTATCTACATCCAGCACACCGCGCAGCATATGTAAGAGGATGt                                        >  2:298934/4‑149 (MQ=255)                                                                                                              tCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGt                                        <  2:117508/149‑1 (MQ=255)                                                                                                                  cGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTg                                    <  2:646505/149‑1 (MQ=255)                                                                                                                      gatgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGcccc                                >  1:480198/1‑149 (MQ=255)                                                                                                                      gatgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGcccc                                >  3:146917/1‑149 (MQ=255)                                                                                                                        tgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAg                              >  2:270716/1‑149 (MQ=255)                                                                                                                           tatGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTc                           >  2:142593/1‑149 (MQ=255)                                                                                                                             tgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCAc                         >  4:8526/1‑149 (MQ=255)                                                                                                                             tgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCAc                         >  2:463798/1‑149 (MQ=255)                                                                                                                                  aaTGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGt                    >  3:315341/1‑149 (MQ=255)                                                                                                                                  aaTGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGt                    <  4:223104/149‑1 (MQ=255)                                                                                                                                  aaTGACCGGGGAGTATCAGCTACGCCCCGGTTTATTGATTGCCTTCGTAATTGCGTCGGTAATTTCGCGGACGTGACACCGTGTCTCTATCTTCCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGGATTGCCCCAGTTCACGCTGt                    <  2:37971/149‑1 (MQ=255)                                                                                                                                   aTGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGtt                   <  4:765712/149‑1 (MQ=255)                                                                                                                                       ccGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGgc               <  4:326618/149‑1 (MQ=255)                                                                                                                                       ccGAGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGCAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCACCAGTTCACGCTGTTCGgc               <  4:25946/149‑1 (MQ=255)                                                                                                                                        cGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGgcg              >  3:617079/1‑149 (MQ=255)                                                                                                                                         ggggAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGgcgc             >  3:390854/1‑149 (MQ=255)                                                                                                                                            gAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGcgcgcg          <  3:550877/149‑1 (MQ=255)                                                                                                                                               tATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCa       >  1:503714/1‑149 (MQ=255)                                                                                                                                                    gCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATaa  <  4:762281/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATAA  >  NC_000913/1666322‑1666617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑