Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,679,400 G→C 9.2% intergenic (+29/‑157) ydgH → / → ydgI DUF1471 family periplasmic protein/putative arginine/ornithine antiporter transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,679,4000GC 9.2% 295.4 / 18.3 89intergenic (+29/‑157)ydgH/ydgIDUF1471 family periplasmic protein/putative arginine/ornithine antiporter transporter
Reads supporting (aligned to +/- strand):  ref base G (39/41);  new base C (3/5);  total (42/47)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01

AAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATC  >  NC_000913/1679253‑1679545
                                                                                                                                                   |                                                                                                                                                 
aaGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGTTGCTAAGTATTACCACATCAACCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGa                                                                                                                                                  <  2:142486/149‑1 (MQ=255)
 aGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGAt                                                                                                                                                 <  1:76141/149‑1 (MQ=255)
   tctcCTATCAGGTTGCGCAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAATGCGACTTGATgc                                                                                                                                               >  2:755964/1‑149 (MQ=255)
   tctcCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATgc                                                                                                                                               >  1:611416/1‑149 (MQ=255)
    gcccTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATgcc                                                                                                                                              <  2:58936/147‑1 (MQ=255)
    ctcATATCGGGTTGCAAAGCGTGCCGCGAAAAAAGGTGCTAAGTATTACCACATAACCCACCAGCAGCAGGCACGTGGTAATAATATGACCGTCAGCGCAGATCTGTATAAATCACTATTCAGTTACTCCTATCAGGCGGCTTAATgcc                                                                                                                                              <  4:381896/149‑1 (MQ=255)
     tcCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATgccg                                                                                                                                             >  1:473283/1‑149 (MQ=255)
     tcCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATgccg                                                                                                                                             >  1:525243/1‑149 (MQ=255)
         aTCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCtt                                                                                                                                         <  4:631845/149‑1 (MQ=255)
          tCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCttt                                                                                                                                        >  1:350736/1‑149 (MQ=255)
           cAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCACGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAACGCGGCTTGATGCCGCCTTTc                                                                                                                                       <  4:839154/149‑1 (MQ=255)
            aGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCATCAAGCCGCCttata                                                                                                                                      <  3:764088/149‑4 (MQ=255)
            aGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCATCAAGCCGCCttata                                                                                                                                      <  4:438144/149‑4 (MQ=255)
                   gAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCAttttt                                                                                                                               >  4:340589/1‑149 (MQ=255)
                    aaaCGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTa                                                                                                                              <  3:295171/149‑1 (MQ=255)
                     aaCGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAg                                                                                                                             <  1:679451/149‑1 (MQ=255)
                     aaCGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAg                                                                                                                             <  2:281145/149‑1 (MQ=255)
                       cGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTc                                                                                                                           <  1:238233/149‑1 (MQ=255)
                        gTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCGGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATATATAACTATTCAGTTACGCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTATAGTca                                                                                                                          >  1:856324/1‑149 (MQ=255)
                            cgcgAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGCCCGTGAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACaaa                                                                                                                      <  3:834228/149‑1 (MQ=255)
                              cgAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAAtt                                                                                                                    <  3:832573/149‑1 (MQ=255)
                               gaagaaAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAAttt                                                                                                                   <  2:694108/149‑1 (MQ=255)
                                          cTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCa                                                                                                        >  3:698322/1‑149 (MQ=255)
                                           tAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCAt                                                                                                       <  3:848281/149‑1 (MQ=255)
                                              gTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgc                                                                                                    >  1:596931/1‑149 (MQ=255)
                                              gTATTACCACATCACCCGCCAGTGACAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCATCAAGCCGCCttata                                                                                                                                      <  3:173847/115‑4 (MQ=255)
                                              gTATTACCACATCACCCGCCAGTGACAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCATCAAGCCGCCttata                                                                                                                                      >  4:173847/1‑112 (MQ=255)
                                               tATTACCACATCACCCGCCAGTAGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGTCCATtgca                                                                                                   <  4:643989/149‑1 (MQ=255)
                                                  tACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgcatgc                                                                                                >  4:468270/1‑149 (MQ=255)
                                                    ccACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAg                                                                                              >  3:386018/1‑149 (MQ=255)
                                                    ccACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAg                                                                                              >  3:404145/1‑149 (MQ=255)
                                                    ccACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAg                                                                                              >  4:182019/1‑149 (MQ=255)
                                                     cacaTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTa                                                                                                                              >  3:865084/1‑116 (MQ=255)
                                                     cacaTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTa                                                                                                                              <  4:865084/116‑1 (MQ=255)
                                                      acaTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCATCAAGCCGCCtta                                                                                                                                        <  1:238878/105‑2 (MQ=255)
                                                      acaTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCATCAAGCCGCCtta                                                                                                                                        >  2:238878/1‑104 (MQ=255)
                                                        aTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGacac                                                                                          <  4:270819/149‑1 (MQ=255)
                                                           aCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACAtc                                                                                       <  3:823747/149‑1 (MQ=255)
                                                                ccAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTc                                                                                  <  2:473283/149‑1 (MQ=255)
                                                                     ggCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTaaa                                                                             >  4:126075/1‑149 (MQ=255)
                                                                         ggAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATcc                                                                         <  3:468270/149‑1 (MQ=255)
                                                                           aaCGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCcg                                                                       <  3:217027/149‑1 (MQ=255)
                                                                            aCGTGGTATTAACCTAACCGTACGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAACGCGGCTTGATGCCGCCTTTCGCATTTTTTGTCACAAATTTCCCCGCGGCCATTGCATGCAGACACATCTCTTCGTAAAGTCCcgc                                                                      <  2:513336/149‑1 (MQ=255)
                                                                                   aataaCCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGAt                                                                                                                                                 >  3:240795/1‑67 (MQ=255)
                                                                                   aataaCCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGAt                                                                                                                                                 <  4:240795/67‑1 (MQ=255)
                                                                                   aataaCCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAg                                                               >  3:861886/1‑149 (MQ=255)
                                                                                      aaCCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTgg                                                            >  4:440746/1‑149 (MQ=255)
                                                                                          tGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCaaa                                                        >  2:810241/1‑149 (MQ=255)
                                                                                          tGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCaaa                                                        <  1:535553/149‑1 (MQ=255)
                                                                                               gTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCa                                                   >  4:881918/1‑149 (MQ=255)
                                                                                                     gcAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTa                                             >  1:402480/1‑149 (MQ=255)
                                                                                                      cAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTAt                                            <  3:780706/149‑1 (MQ=255)
                                                                                                       aGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATg                                           >  4:708687/1‑149 (MQ=255)
                                                                                                          tCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGt                                        <  1:810241/149‑1 (MQ=255)
                                                                                                            tGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGttt                                      >  4:461370/1‑149 (MQ=255)
                                                                                                                 aaataaCTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGccc                                 >  3:672050/1‑149 (MQ=255)
                                                                                                                  aataaCTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCt                                >  1:448030/1‑149 (MQ=255)
                                                                                                                    taaCTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGa                                                                                             <  3:704041/86‑1 (MQ=255)
                                                                                                                    taaCTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGa                                                                                             >  4:704041/1‑86 (MQ=255)
                                                                                                                    taaCTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTaa                              >  4:536960/1‑149 (MQ=255)
                                                                                                                      aCTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAata                            <  3:527023/149‑1 (MQ=255)
                                                                                                                        tATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAatat                           <  1:262239/148‑1 (MQ=255)
                                                                                                                        tATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAatat                           >  2:262239/1‑148 (MQ=255)
                                                                                                                        tATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtatt                          >  3:715902/1‑149 (MQ=255)
                                                                                                                        tATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtatt                          >  4:78602/1‑149 (MQ=255)
                                                                                                                        tATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtatt                          >  2:532165/1‑149 (MQ=255)
                                                                                                                          ttCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACACATTTCCCCGCGACCATTGCATGCAGACGCATCTCTTCGTAAAATCCCGAGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCTTAAtattta                        <  2:856324/149‑1 (MQ=255)
                                                                                                                          ttCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtattta                        <  3:340589/149‑1 (MQ=255)
                                                                                                                          ttCACTTACTCCTATAAGTCGGCTTGATGCCAGCTTTCGCATTTATGGTCACAAATTTCCCTGCGACCATTGCAGGCAGACACTTCTCGTCGTAAAAGCCCGCGCTTCAGTGGCAAACATCATTCTTATGCGTTTTGCCCTAAtattaa                        <  2:24233/149‑3 (MQ=255)
                                                                                                                           tCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtatttat                       <  1:574299/149‑1 (MQ=255)
                                                                                                                           tCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtatttat                       <  2:816582/149‑1 (MQ=255)
                                                                                                                           tCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAAGCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtatttat                       <  2:121422/149‑1 (MQ=255)
                                                                                                                             aGTTACTCCTATAAGGCGGCTTGATGCCGCCTGTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTTttc                     >  4:75281/1‑149 (MQ=255)
                                                                                                                              gTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTAttct                    >  3:36613/1‑149 (MQ=255)
                                                                                                                               ttACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTAttctt                   <  3:461370/149‑1 (MQ=255)
                                                                                                                                tACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTAttcttc                  <  2:448030/149‑1 (MQ=255)
                                                                                                                                 aCTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATtcttct                 <  1:291903/149‑1 (MQ=255)
                                                                                                                                 aCTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATtcgtct                 <  1:771327/149‑1 (MQ=255)
                                                                                                                                  cTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTcttctt                <  4:117338/149‑1 (MQ=255)
                                                                                                                                   tCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTa               >  2:576403/1‑149 (MQ=255)
                                                                                                                                     cTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATc             >  3:818301/1‑149 (MQ=255)
                                                                                                                                     cTATAAGGCGGCTTGATGCCGCCTTTCGCATCTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCGCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATATATTCTTCTTATc             >  2:499551/1‑149 (MQ=255)
                                                                                                                                      tataAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCa            >  2:585826/1‑149 (MQ=255)
                                                                                                                                       gaaaGGCGGCATCAAGCCGCCTTTCGCATTTTTCGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtatttatt                      >  3:751139/3‑138 (MQ=255)
                                                                                                                                       gaaaGGCGGCATCAAGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtatttatt                      <  4:751139/136‑1 (MQ=255)
                                                                                                                                             cggcTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTa     <  1:298441/149‑1 (MQ=255)
                                                                                                                                              ggcTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTaa    >  3:815033/1‑149 (MQ=255)
                                                                                                                                              ggcTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTaa    >  2:486080/1‑149 (MQ=255)
                                                                                                                                                cTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATc  <  4:861886/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATC  >  NC_000913/1679253‑1679545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: