Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,944,240 T→G 5.6% intergenic (+41/+106) znuB → / ← ruvB zinc ABC transporter permease/ATP‑dependent DNA helicase, component of RuvABC resolvasome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,944,2400TG 5.6% 250.0 / 4.4 71intergenic (+41/+106)znuB/ruvBzinc ABC transporter permease/ATP‑dependent DNA helicase, component of RuvABC resolvasome
Reads supporting (aligned to +/- strand):  ref base T (33/34);  new base G (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCC  >  NC_000913/1944093‑1944388
                                                                                                                                                   |                                                                                                                                                    
gCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCtt                                                                                                                                                     <  1:12369/149‑1 (MQ=255)
gCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCtt                                                                                                                                                     <  4:676212/149‑1 (MQ=255)
gCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCtt                                                                                                                                                     <  2:60355/149‑1 (MQ=255)
  ggTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTAt                                                                                                                                                   <  3:685249/149‑1 (MQ=255)
    tttAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATcc                                                                                                                                                 <  2:562531/149‑1 (MQ=255)
       aaCCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCGCTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGAc                                                                                                                                              <  4:146368/149‑1 (MQ=255)
          cTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTa                                                                                                                                           >  1:593162/1‑149 (MQ=255)
          cTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTa                                                                                                                                           >  2:466871/1‑149 (MQ=255)
           ttttCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAcaaat                                                                                                                                          <  2:635656/149‑5 (MQ=255)
                 gcgGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCg                                                                                                                                    >  3:775651/1‑149 (MQ=255)
                 gcgGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCg                                                                                                                                    >  4:481217/1‑149 (MQ=255)
                  cgGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCgg                                                                                                                                   <  1:509452/149‑1 (MQ=255)
                     tttACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTAt                                                                                                                                <  2:326713/149‑1 (MQ=255)
                         cGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGCGCGCCTTATCCGTCCTACGGTTCGGTATctct                                                                                                                            <  1:26901/149‑1 (MQ=255)
                         cGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATctct                                                                                                                            <  3:688207/149‑1 (MQ=255)
                         cGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATctct                                                                                                                            <  3:481217/149‑1 (MQ=255)
                          gATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTg                                                                                                                           >  3:331030/1‑149 (MQ=255)
                           aTACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTgg                                                                                                                          <  4:435029/149‑1 (MQ=255)
                           aTACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTgg                                                                                                                          <  4:825471/149‑1 (MQ=255)
                             aCGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTa                                                                                                                        <  2:796965/149‑1 (MQ=255)
                              cGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATATGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAg                                                                                                                       >  1:641238/1‑149 (MQ=255)
                                  ggcggGTCCGACGGTGAAGATATATGCGGCACCGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTACCGCTCAACACATTTGTCTGATGCGGCGCCAGCGCCTTAACCGACCTACGGTTCGGTCTCTCTCGTAGTCCt                                                                                                                   <  4:411814/149‑1 (MQ=255)
                                    cggGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTgg                                                                                                                 <  4:649409/149‑1 (MQ=255)
                                       gTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTaa                                                                                                              <  2:308596/149‑1 (MQ=255)
                                       gTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTaa                                                                                                              <  2:427732/149‑1 (MQ=255)
                                       gTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTaa                                                                                                              >  1:308596/1‑149 (MQ=255)
                                         ccGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGa                                                                                                            <  4:340261/149‑1 (MQ=255)
                                         ccGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGATAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGa                                                                                                            <  3:29041/149‑1 (MQ=255)
                                          cGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGCTGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGAc                                                                                                           <  3:754793/149‑1 (MQ=255)
                                          cGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGAc                                                                                                           <  4:775651/149‑1 (MQ=255)
                                           gTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGAcg                                                                                                          >  1:203440/1‑149 (MQ=255)
                                                 ggtgCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACa                                                                                                    <  3:861872/149‑1 (MQ=255)
                                                     cTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGt                                                                                                <  3:782393/149‑1 (MQ=255)
                                                      tATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTc                                                                                               <  1:106051/149‑1 (MQ=255)
                                                      tATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTc                                                                                               >  2:177330/1‑149 (MQ=255)
                                                       aTGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCg                                                                                              >  3:440989/1‑149 (MQ=255)
                                                               cACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGc                                                                                      <  3:874185/149‑1 (MQ=255)
                                                                aCTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCa                                                                                     >  1:339567/1‑149 (MQ=255)
                                                                    ttatttatTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATAtt                                                                                 <  2:339567/149‑1 (MQ=255)
                                                                           ttCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTg                                                                          <  4:440989/149‑1 (MQ=255)
                                                                              tcAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCg                                                                       >  2:724520/1‑149 (MQ=255)
                                                                              tcAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCg                                                                       >  1:835111/1‑149 (MQ=255)
                                                                                 gTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGAt                                                                    >  2:832564/1‑149 (MQ=255)
                                                                                    tgatgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcg                                                                 >  3:304087/1‑149 (MQ=255)
                                                                                           aaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCgcgag                                                          >  4:669385/1‑149 (MQ=255)
                                                                                             aGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCgcgagcg                                                        >  4:699241/1‑149 (MQ=255)
                                                                                             aGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCgcgagcg                                                        >  4:684143/1‑149 (MQ=255)
                                                                                               cAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGAc                                                      <  2:407552/149‑1 (MQ=255)
                                                                                                   ccAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAAt                                                  >  1:489801/1‑149 (MQ=255)
                                                                                                    cAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATc                                                 >  2:584707/1‑149 (MQ=255)
                                                                                                      gCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCg                                               >  2:435407/1‑149 (MQ=255)
                                                                                                       cTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGa                                              <  4:304087/149‑1 (MQ=255)
                                                                                                         aaTCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACt                                            >  2:230144/1‑149 (MQ=255)
                                                                                                         aaTCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACt                                            >  1:253505/1‑149 (MQ=255)
                                                                                                            cTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTAc                                         <  3:601827/149‑1 (MQ=255)
                                                                                                             tATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACg                                        <  2:303972/149‑1 (MQ=255)
                                                                                                             tATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACg                                        >  3:187562/1‑149 (MQ=255)
                                                                                                                       cacaTTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTgg                              >  2:49300/1‑149 (MQ=255)
                                                                                                                        acaTTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTggc                             >  3:464768/1‑149 (MQ=255)
                                                                                                                          aTTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTggcgg                           >  4:110261/1‑149 (MQ=255)
                                                                                                                               tCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTa                      <  2:203440/149‑1 (MQ=255)
                                                                                                                               tCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTa                      >  1:504772/1‑149 (MQ=255)
                                                                                                                                   ataaggcgCTCGCGCCGCATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGcc                  >  4:538607/5‑149 (MQ=39)
                                                                                                                                      cggcgCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCaaa               <  1:466871/149‑1 (MQ=255)
                                                                                                                                       ggcgCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAg              >  2:493876/1‑149 (MQ=255)
                                                                                                                                          gcgAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGa           <  3:202625/149‑1 (MQ=255)
                                                                                                                                           cgAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGAt          <  4:393108/149‑1 (MQ=255)
                                                                                                                                            tcgcgcCGCATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGAtt         >  4:696653/3‑149 (MQ=255)
                                                                                                                                            gAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGAtt         >  2:453343/1‑149 (MQ=255)
                                                                                                                                            gAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGAtt         <  2:504772/149‑1 (MQ=255)
                                                                                                                                            gAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGAtt         <  3:475794/149‑1 (MQ=255)
                                                                                                                                             aGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTc        >  1:451698/1‑149 (MQ=255)
                                                                                                                                                  cTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGc   >  4:40656/1‑149 (MQ=255)
                                                                                                                                                  cTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGc   >  4:787275/1‑149 (MQ=255)
                                                                                                                                                   ttATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGcc  >  1:95560/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCC  >  NC_000913/1944093‑1944388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: