Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,954,444 A→C 5.7% intergenic (+31/+134) cmoB → / ← torZ tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent/trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,954,4440AC 5.7% 306.1 / 9.2 88intergenic (+31/+134)cmoB/torZtRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent/trimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (41/42);  new base C (2/3);  total (43/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01

ACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGTCTTACGCCTGAACA  >  NC_000913/1954297‑1954590
                                                                                                                                                   |                                                                                                                                                  
aCCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGaa                                                                                                                                                   >  3:455035/1‑149 (MQ=255)
 ccGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAAc                                                                                                                                                  >  4:238947/1‑149 (MQ=255)
 ccGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAAc                                                                                                                                                  <  4:163865/149‑1 (MQ=255)
      tggatggTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATAAGGCGTTCACGccgc                                                                                                                                             >  1:720371/1‑147 (MQ=255)
         atggTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATAAGGCGTTCACGccgcatc                                                                                                                                          <  4:30207/149‑6 (MQ=255)
         atggTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATAAGGCGTTCACGccgcatc                                                                                                                                          <  2:720371/149‑6 (MQ=255)
              tACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATAAGGCGTTCACGCCGCATCCGGca                                                                                                                                     <  2:320540/149‑2 (MQ=255)
               aCCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                                                                                                    >  3:687791/1‑149 (MQ=255)
                         tGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCtt                                                                                                                          <  1:871358/149‑1 (MQ=255)
                         tGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCtt                                                                                                                          <  4:624080/149‑1 (MQ=255)
                         tGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCtt                                                                                                                          <  3:238947/149‑1 (MQ=255)
                         tGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCtt                                                                                                                          >  1:738331/1‑149 (MQ=255)
                           gCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGc                                                                                                                        >  3:249159/1‑149 (MQ=255)
                           gCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGc                                                                                                                        <  4:826628/149‑1 (MQ=255)
                           gCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGc                                                                                                                        <  4:320394/149‑1 (MQ=255)
                           gCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGGATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGc                                                                                                                        >  3:635154/1‑149 (MQ=255)
                                tttCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt                                                                                                                   >  2:77864/1‑149 (MQ=255)
                                 ttCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTc                                                                                                                  <  4:77435/149‑1 (MQ=255)
                                   ccTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa                                                                                                                <  4:139529/149‑1 (MQ=255)
                                   ccTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa                                                                                                                <  4:760747/149‑1 (MQ=255)
                                   ccTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa                                                                                                                <  2:723933/149‑1 (MQ=255)
                                        aCCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAatatat                                                                                                           <  2:101109/149‑1 (MQ=255)
                                         cccACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATAtt                                                                                                          <  1:125206/149‑1 (MQ=255)
                                          ccACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTg                                                                                                         >  2:373594/1‑149 (MQ=255)
                                            acaTGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCa                                                                                                       <  4:465432/149‑1 (MQ=255)
                                            acaTGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCa                                                                                                       >  1:164403/1‑149 (MQ=255)
                                             caTGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAg                                                                                                      <  3:164774/149‑1 (MQ=255)
                                              aTGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg                                                                                                     >  2:592963/1‑149 (MQ=255)
                                              aTGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGCGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg                                                                                                     <  3:94297/149‑1 (MQ=255)
                                                gATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGa                                                                                                   >  3:777632/1‑149 (MQ=255)
                                                  tCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACt                                                                                                 >  4:521656/1‑149 (MQ=255)
                                                  tCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGAAAAGGTCTGGTATTACTGCCGGATGCAGCGTGAACACCTTATCCGGCCTACCAAACATTGCTAATTCAATATATTGCAGGGACt                                                                                                 >  2:824369/1‑149 (MQ=39)
                                                   ccGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTa                                                                                                >  1:191894/1‑149 (MQ=255)
                                                       gTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTa                                                                                            <  2:158372/149‑1 (MQ=255)
                                                         aaaaCGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAgg                                                                                          <  4:498042/149‑1 (MQ=255)
                                                           aaCGGTGGAAGGTTATCCGGCACCTCAACGCGCGTTGCTGATGGCGCGCAAGCCGTAGAGGTCTGGCATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGcc                                                                                        >  4:196341/1‑149 (MQ=37)
                                                           aaCGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGcc                                                                                        >  3:27689/1‑149 (MQ=255)
                                                           aaCGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGcc                                                                                        <  3:521656/149‑1 (MQ=255)
                                                            aCGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt                                                                                       >  4:825400/1‑149 (MQ=255)
                                                            aCGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt                                                                                       >  3:534238/1‑149 (MQ=255)
                                                              ggTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGa                                                                                     <  3:405891/149‑1 (MQ=255)
                                                               gTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGAt                                                                                    <  4:151264/149‑1 (MQ=255)
                                                                  gAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGt                                                                                             >  4:36098/1‑137 (MQ=255)
                                                                  gAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGt                                                                                             >  4:327244/1‑137 (MQ=255)
                                                                  gAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGt                                                                                             <  3:327244/137‑1 (MQ=255)
                                                                  gAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGt                                                                                             <  3:36098/137‑1 (MQ=255)
                                                                  gAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg                                                                                 <  4:27689/149‑1 (MQ=255)
                                                                      gTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATa                                                                             <  2:100590/149‑1 (MQ=255)
                                                                       ttATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAg                                                                            <  1:281028/149‑1 (MQ=255)
                                                                         aTCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                                                                          >  3:87994/1‑149 (MQ=255)
                                                                                  cTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCa                                                                 >  4:619698/1‑149 (MQ=255)
                                                                                  cTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCa                                                                 >  1:79777/1‑149 (MQ=255)
                                                                                   tAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAg                                                                >  3:10237/1‑149 (MQ=255)
                                                                                          gcgGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACg                                                         >  4:594253/1‑149 (MQ=255)
                                                                                             gTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGttt                                                      <  3:600276/149‑1 (MQ=255)
                                                                                              tGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTg                                                     >  1:327965/1‑149 (MQ=255)
                                                                                                cTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCa                                                   <  3:619698/149‑1 (MQ=255)
                                                                                                  gATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATa                                                 >  3:15255/1‑149 (MQ=255)
                                                                                                   aTTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATaa                                                >  4:550490/1‑149 (MQ=255)
                                                                                                    ttGCGCGCAAATTGTAAATGTTTGTTATTACTGCAGGATGAGGGGTTAACGCTTTTTTCGGCGTACAAGACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGGTAATGAGGCAGCTTTACGTGTGCACAAc                                               <  2:600905/149‑1 (MQ=16)
                                                                                                        gcgcAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCa                                           <  2:2542/149‑1 (MQ=255)
                                                                                                        gcgcAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCa                                           <  2:335193/149‑1 (MQ=255)
                                                                                                        gcgcAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCa                                           >  3:522807/1‑149 (MQ=255)
                                                                                                         cgcAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAg                                          >  1:455084/1‑149 (MQ=255)
                                                                                                           cAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                      >  1:152405/1‑55 (MQ=255)
                                                                                                           cAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                      <  2:152405/55‑1 (MQ=255)
                                                                                                             aGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGcc                                      <  4:534238/149‑1 (MQ=255)
                                                                                                               ccGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCg                                    >  3:478978/1‑149 (MQ=255)
                                                                                                                  tAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGttt                                 <  4:442285/149‑1 (MQ=255)
                                                                                                                     aGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCg                              <  3:196341/149‑1 (MQ=255)
                                                                                                                     aGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCg                              <  4:687791/149‑1 (MQ=255)
                                                                                                                     aGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCg                              >  4:622524/1‑149 (MQ=255)
                                                                                                                      ggTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCgg                             <  4:777632/149‑1 (MQ=255)
                                                                                                                       gTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCggg                            <  3:594253/149‑1 (MQ=255)
                                                                                                                        tCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGgc                           >  2:676706/1‑149 (MQ=255)
                                                                                                                          tGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGgcgc                         >  2:363697/1‑149 (MQ=255)
                                                                                                                             tATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGGAAACGGGCGCtga                      <  1:881618/149‑3 (MQ=255)
                                                                                                                               ttACTGCCGGATGCGGCGTGAACACCTGATCCGGCCTACAAAACCTTGCTAATTCGATATGTTGCAGGGACTAGGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTc                    >  2:43907/1‑149 (MQ=25)
                                                                                                                                   tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGt                <  4:249159/149‑1 (MQ=255)
                                                                                                                                   tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGt                >  2:680940/1‑149 (MQ=255)
                                                                                                                                   tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGt                >  1:764186/1‑149 (MQ=255)
                                                                                                                                          taaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGACTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGTCGCTATTCACGTCTTACGc         >  2:810466/4‑149 (MQ=25)
                                                                                                                                          tGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGTCTTACGc         <  4:87994/149‑1 (MQ=255)
                                                                                                                                             ggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAAc                                               >  2:281728/1‑108 (MQ=255)
                                                                                                                                             ggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAAc                                               <  1:281728/108‑1 (MQ=255)
                                                                                                                                               cgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGTCTTACGCCTGaa    <  4:455035/149‑1 (MQ=255)
                                                                                                                                                gTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGTCTTACGCCTGAAc   >  3:299664/1‑149 (MQ=255)
                                                                                                                                                gTGAACGCCCTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCTGGGCGCTATTCACGTCTTACGCCTGaa  >  4:759668/1‑148 (MQ=255)
                                                                                                                                                 tGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGTCTTACGCCTGAACa  >  3:148379/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGTCTTACGCCTGAACA  >  NC_000913/1954297‑1954590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: