Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,981,815 T→A 5.7% R191R (CGA→CGT)  otsB ← trehalose‑6‑phosphate phosphatase, biosynthetic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,981,8150TA 5.7% 305.3 / 7.5 88R191R (CGA→CGT) otsBtrehalose‑6‑phosphate phosphatase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base T (36/47);  new base A (3/2);  total (39/49)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

AAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAA  >  NC_000913/1981668‑1981961
                                                                                                                                                   |                                                                                                                                                  
aaGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTc                                                                                                                                                   >  1:239524/1‑149 (MQ=255)
   ccAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGcc                                                                                                                                                <  3:161689/149‑1 (MQ=255)
   ccAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGcc                                                                                                                                                >  1:793736/1‑149 (MQ=255)
        tCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgtat                                                                                                                                           <  1:675992/149‑4 (MQ=255)
           aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg                                                                                                                                        >  1:252678/1‑149 (MQ=255)
           aGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAg                                                                                                                                        <  3:454292/149‑1 (MQ=255)
             aCATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAggg                                                                                                                                      <  4:447462/149‑1 (MQ=255)
             aCATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAggg                                                                                                                                      <  3:262104/149‑1 (MQ=255)
               aTCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAg                                                                                                                                    >  1:519819/1‑149 (MQ=255)
                tCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGc                                                                                                                                   >  3:129053/1‑149 (MQ=255)
                 ccGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCt                                                                                                                                  >  3:577331/1‑149 (MQ=255)
                       cacCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGc                                                                                                                            <  4:186821/149‑1 (MQ=255)
                          ccGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATa                                                                                                                         >  1:245187/1‑149 (MQ=255)
                          ccGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATa                                                                                                                         >  1:741237/1‑149 (MQ=255)
                             ccAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa                                                                                                                      <  1:860888/149‑1 (MQ=255)
                             ccAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa                                                                                                                      <  1:453111/149‑1 (MQ=255)
                             ccAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa                                                                                                                      <  3:209132/149‑1 (MQ=255)
                             ccAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa                                                                                                                      <  2:301571/149‑1 (MQ=255)
                               aGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGc                                                                                                                    <  1:318167/149‑1 (MQ=255)
                                gTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCt                                                                                                                   <  1:659692/149‑1 (MQ=255)
                                gTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCt                                                                                                                   <  2:884840/149‑1 (MQ=255)
                                 tCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTg                                                                                                                  >  2:599529/1‑149 (MQ=255)
                                     cATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAAt                                                                                                              <  1:690107/149‑1 (MQ=255)
                                         atgCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGcc                                                                                                          <  1:283528/149‑1 (MQ=255)
                                                   tGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCAACGGTAAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTAc                                                                                                <  3:833527/149‑1 (MQ=255)
                                                   tGCACCTGTGCCAATTTTTACTGACATTCCGCCAAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTAc                                                                                                <  1:500287/149‑1 (MQ=255)
                                                    gCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACt                                                                                               <  3:843391/149‑1 (MQ=255)
                                                      aCCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCt                                                                                                                              <  3:507506/116‑1 (MQ=255)
                                                      aCCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTgg                                                                                             >  3:543893/1‑149 (MQ=255)
                                                      aCCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGATAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCt                                                                                                                              >  4:507506/1‑116 (MQ=255)
                                                        cTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTa                                                                                           >  2:114230/1‑149 (MQ=255)
                                                        cTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTa                                                                                           <  3:869554/149‑1 (MQ=255)
                                                        cTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgta                                                                                                                                            <  3:671481/100‑3 (MQ=255)
                                                        cTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgta                                                                                                                                            >  4:671481/1‑98 (MQ=255)
                                                         tgtgCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTAc                                                                                          <  3:27054/149‑1 (MQ=255)
                                                               aaTTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCg                                                                                    <  1:599529/149‑1 (MQ=255)
                                                               aaTTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTCTACCGGTACCTCTCg                                                                                    <  3:515021/149‑1 (MQ=255)
                                                                aTTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCgg                                                                                   <  2:537926/149‑1 (MQ=255)
                                                                 tttttACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGt                                                                                  <  4:45345/149‑1 (MQ=255)
                                                                    ttACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGAAAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTg                                                                               <  2:718047/149‑1 (MQ=255)
                                                                     tACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGa                                                                              <  4:74130/149‑1 (MQ=255)
                                                                      aCTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAt                                                                             <  3:867472/149‑1 (MQ=255)
                                                                      aCTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAt                                                                             <  3:92570/149‑1 (MQ=255)
                                                                      aCTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAt                                                                             <  1:749104/149‑1 (MQ=255)
                                                                      aCTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTCACTGGTACCTCTCGGTTTGAt                                                                             >  3:522492/1‑149 (MQ=255)
                                                                        tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtct                                                                           >  1:37604/1‑149 (MQ=255)
                                                                        tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtct                                                                           >  3:605196/1‑149 (MQ=255)
                                                                        tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtct                                                                           >  1:752641/1‑149 (MQ=255)
                                                                        tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtct                                                                           >  3:784628/1‑149 (MQ=255)
                                                                        tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtct                                                                           >  1:548207/1‑149 (MQ=255)
                                                                         gACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtctc                                                                          >  2:225894/1‑149 (MQ=255)
                                                                         gACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtctc                                                                          <  3:727753/149‑1 (MQ=255)
                                                                         gACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtctc                                                                          <  2:274131/149‑1 (MQ=255)
                                                                           cATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGa                                                                        <  4:325193/149‑1 (MQ=255)
                                                                           cATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGa                                                                        <  2:648195/149‑1 (MQ=255)
                                                                              tCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGacaa                                                                     >  3:442782/1‑149 (MQ=255)
                                                                                ttcgcAGTCGTTAACCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGacaaca                                                                   >  3:654574/5‑149 (MQ=255)
                                                                                   ccAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAacacac                                                                <  2:458583/149‑1 (MQ=255)
                                                                                     aGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACtt                                                              >  1:51617/1‑149 (MQ=255)
                                                                                           ttAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCctg                                                        >  4:187093/1‑149 (MQ=255)
                                                                                             aaCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCctgct                                                      <  2:548207/149‑1 (MQ=255)
                                                                                                 aCTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTaa                                                  <  4:543893/149‑1 (MQ=255)
                                                                                                   tGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACg                                                >  4:658953/1‑149 (MQ=255)
                                                                                                      gAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCa                                             >  3:158428/1‑149 (MQ=255)
                                                                                                          ccAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTtg                                         >  1:764435/1‑149 (MQ=255)
                                                                                                           cAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTtgt                                        <  2:793736/149‑1 (MQ=255)
                                                                                                            aGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTtgtg                                       <  4:129053/149‑1 (MQ=255)
                                                                                                             gATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTgg                                      <  2:253425/149‑1 (MQ=255)
                                                                                                                tCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCa                                   <  4:577331/149‑1 (MQ=255)
                                                                                                                  aTCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGa                                 >  2:255329/1‑149 (MQ=255)
                                                                                                                    cGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATc                               >  2:519797/1‑149 (MQ=255)
                                                                                                                     ggTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCt                              >  4:225461/1‑149 (MQ=255)
                                                                                                                       ttAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTAc                                                                                          <  4:835458/87‑1 (MQ=255)
                                                                                                                       ttAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTAc                                                                                          >  3:835458/1‑87 (MQ=255)
                                                                                                                       ttAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTCCTGTAACGCCATTTGTGGCCAGATCTGa                            <  4:516666/149‑1 (MQ=255)
                                                                                                                           atcatcGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTaa                        >  3:145767/1‑149 (MQ=255)
                                                                                                                           atcatcGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTaa                        <  2:75186/149‑1 (MQ=255)
                                                                                                                           atcatcGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTaa                        <  2:675973/149‑1 (MQ=255)
                                                                                                                              atcGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATAc                     >  4:422113/1‑149 (MQ=255)
                                                                                                                                   ccAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTgc                >  4:418884/1‑149 (MQ=255)
                                                                                                                                     aGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTgcgc              >  3:326146/1‑149 (MQ=255)
                                                                                                                                      ctttatcGGGCGAACGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGCCCAGATCTGAGTAATACGTTGCGCt             >  1:6655/7‑149 (MQ=255)
                                                                                                                                       tttatcGGGCGAACGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTa            >  3:595835/6‑149 (MQ=255)
                                                                                                                                         aTACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAAt          >  2:823595/1‑149 (MQ=255)
                                                                                                                                          tACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATg         <  4:145767/149‑1 (MQ=255)
                                                                                                                                            cGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTc       <  2:252678/149‑1 (MQ=255)
                                                                                                                                              ggCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTAGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCAt     >  3:615586/1‑149 (MQ=255)
                                                                                                                                                 gTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTaa  <  4:504272/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAA  >  NC_000913/1981668‑1981961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑