Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,025,579 T→C 6.1% S23S (AGT→AGC) ‡ yedP → putative mannosyl‑3‑phosphoglycerate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,025,5790TC 6.1% 210.1 / 3.5 65S23S (AGT→AGC) ‡yedPputative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (34/27);  new base C (2/2);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

GGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATG  >  NC_000913/2025431‑2025727
                                                                                                                                                    |                                                                                                                                                    
gggATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGt                                                                                                                                                      >  1:177348/1‑149 (MQ=255)
  gATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTa                                                                                                                                                    <  3:460308/149‑1 (MQ=255)
      gCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAACTATGAc                                                                                                                                                <  4:451997/149‑1 (MQ=255)
       cTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACt                                                                                                                                               <  1:375264/149‑1 (MQ=255)
        tCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCTACTATGTCTTCAATTCAACTACCACTACTGGTTTTTCGCGATCTTGATGGCACCCTGCTGGACACTCACAGTTATGACtt                                                                                                                                              >  4:572688/1‑148 (MQ=255)
        tCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTg                                                                                                                                              >  3:240393/1‑149 (MQ=255)
          ccGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGCCAGTCATAACTATGACTgtc                                                                                                                                            <  2:669460/149‑3 (MQ=255)
          ccGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGc                                                                                                                                            >  4:210822/1‑149 (MQ=255)
                   tcatcTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAg                                                                                                                                   >  4:630316/1‑149 (MQ=255)
                   tcatcTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAg                                                                                                                                   >  1:238278/1‑149 (MQ=255)
                        tttGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGccccc                                                                                                                              >  4:620824/1‑149 (MQ=255)
                        tttGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGccccc                                                                                                                              <  1:410456/149‑1 (MQ=255)
                        tttGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGccccc                                                                                                                              <  2:668464/149‑1 (MQ=255)
                         ttGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCt                                                                                                                             <  3:784954/149‑1 (MQ=255)
                             tAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCt                                                                                                                         <  3:459987/149‑1 (MQ=255)
                               aTTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCa                                                                                                                       >  4:52606/1‑149 (MQ=255)
                                tttGTGCGGTTTTGAGAATCATCCTGGTAAGAGGTGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCAc                                                                                                                      >  2:438773/1‑149 (MQ=255)
                                     gCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGtt                                                                                                                 <  1:699695/149‑1 (MQ=255)
                                        gTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCCCTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTAc                                                                                                              <  1:460477/149‑1 (MQ=255)
                                        gTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTAc                                                                                                              <  3:514195/149‑1 (MQ=255)
                                             gagaATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGaa                                                                                                         <  1:594895/149‑1 (MQ=255)
                                                aaTCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCa                                                                                                      >  3:54685/1‑149 (MQ=255)
                                                       cTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTc                                                                                               >  2:857146/1‑149 (MQ=255)
                                                               aggagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCAtt                                                                                       >  2:614373/1‑149 (MQ=255)
                                                                 gagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATtct                                                                                     <  4:410053/149‑1 (MQ=255)
                                                                    tACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTg                                                                                  <  4:366771/149‑1 (MQ=255)
                                                                      cTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTa                                                                                <  1:796676/149‑1 (MQ=255)
                                                                         cgcgACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCa                                                                             <  3:559008/149‑1 (MQ=255)
                                                                              cTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTaaa                                                                        >  1:898044/1‑149 (MQ=255)
                                                                               tATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTaaaa                                                                       <  2:752551/149‑1 (MQ=255)
                                                                               tATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTaaaa                                                                       >  3:470418/1‑149 (MQ=255)
                                                                               tATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTaaaa                                                                       >  1:505618/1‑149 (MQ=255)
                                                                                aTGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAAc                                                                      <  4:480914/149‑1 (MQ=255)
                                                                                  gTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACAt                                                                    <  3:804406/149‑1 (MQ=255)
                                                                                    tttCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCAACCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCa                                                                  <  3:210822/149‑1 (MQ=255)
                                                                                     ttcaattcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCa                                                                             <  4:502995/137‑1 (MQ=255)
                                                                                     ttcaattcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCa                                                                             >  3:502995/1‑137 (MQ=255)
                                                                                          ttcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGaa                                                            >  3:703486/1‑149 (MQ=255)
                                                                                             aacaacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTa                                                                                <  3:244724/126‑1 (MQ=255)
                                                                                             aacaacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTa                                                                                >  4:244724/1‑126 (MQ=255)
                                                                                               caacCACTACTCGTTTTTAGCGATCTTGATGGCACCCTGCTGAACAGTCATAGTTATGACTGGCAACCGGCTGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCt                                                       <  3:572688/149‑1 (MQ=255)
                                                                                                   cACTACTGGTTTGTAGCGATCTTGATGGCACCGTTCTGCACAGTCATAGTTATGACTCGCAACCGGCAGCCCCCTGGCTCAACCGTTTACGCGAAGAAAATGTTCCCCTCATTGTCCGTAGCAGTAAAACATCAGCGGAAATGCTGTat                                                   >  2:647334/1‑148 (MQ=255)
                                                                                                     ctactGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGTCCCCTGGCTCACCCGTTTCAGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGCAAAACATCAGCGGAAATGCTATACtt                                                 <  4:753200/149‑1 (MQ=255)
                                                                                                         tGGTTTTTAGCGATCTTGAAGGCACCCTGCTGGACAGTCATAGTTATGACTGCCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCaa                                             >  4:749265/1‑149 (MQ=255)
                                                                                                          ggTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCaaa                                            >  3:675317/1‑149 (MQ=255)
                                                                                                            tttttAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCaaaaa                                          >  4:47406/1‑149 (MQ=255)
                                                                                                            tttttAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCaaaaa                                          >  1:171964/1‑149 (MQ=255)
                                                                                                                 aGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGtt                                     <  2:177348/149‑1 (MQ=255)
                                                                                                                  gCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTg                                    <  4:526719/149‑1 (MQ=255)
                                                                                                                   cGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgg                                   <  2:292303/149‑1 (MQ=255)
                                                                                                                     aTCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGtt                                                                                                >  1:440259/1‑86 (MQ=255)
                                                                                                                     aTCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGtt                                                                                                <  2:440259/86‑1 (MQ=255)
                                                                                                                     aTCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgggg                                 >  3:90138/1‑149 (MQ=255)
                                                                                                                        ttGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTa                              >  3:507286/1‑149 (MQ=255)
                                                                                                                           aTGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACaa                           >  2:674451/1‑149 (MQ=255)
                                                                                                                            tGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAATTGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAg                          >  4:74316/1‑149 (MQ=255)
                                                                                                                            tGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAg                          >  1:535849/1‑149 (MQ=255)
                                                                                                                                 cccTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTa                     >  2:348686/1‑149 (MQ=255)
                                                                                                                                       tgGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTg               >  4:482505/1‑149 (MQ=255)
                                                                                                                                       tgGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTg               >  2:157263/1‑149 (MQ=255)
                                                                                                                                          ccAGTCATAACTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGAtt            >  1:109266/2‑149 (MQ=255)
                                                                                                                                          ccAGTCATAACTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGAtt            >  2:69137/2‑149 (MQ=255)
                                                                                                                                            agccATAGTTATGACTGGCAACCGACAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGc          >  4:156251/4‑149 (MQ=255)
                                                                                                                                              tCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAACCATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCg        >  4:298865/1‑149 (MQ=255)
                                                                                                                                              tCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCaaaaaa                                         <  4:538175/116‑1 (MQ=255)
                                                                                                                                              tCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCaaaaaa                                         >  3:538175/1‑116 (MQ=255)
                                                                                                                                                    ttATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATg  >  4:264038/1‑149 (MQ=255)
                                                                                                                                                    ttATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATg  <  3:787823/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGGATCGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATG  >  NC_000913/2025431‑2025727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: